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    • 70. 发明申请
    • RECOMBINANT MATRIX PROTEIN OF NIPAH VIRUS
    • NIPAH病毒的重组基质蛋白
    • WO2011056060A3
    • 2011-12-15
    • PCT/MY2010000211
    • 2010-10-19
    • UNIV PUTRA MALAYSIATAN WEN SIANGSUBRAMANIAM SENTHIL KUMARTEY BENG TIHAMID MUHAJIR
    • TAN WEN SIANGSUBRAMANIAM SENTHIL KUMARTEY BENG TIHAMID MUHAJIR
    • C07K14/115A61K39/155C12N15/45C12N15/63C12Q1/70
    • C07K16/1027A61K39/00C07K14/005C12N2760/18222
    • The present invention provides a recombinant matrix protein of Nipah virus containing matrix protein of Nipah virus which includes at least one His tag and myc epitope. Further, the present invention also provides a recombinant plasmid including recombinant matrix protein of Nipah virus. Also provided is a method for producing recombinant matrix protein of Nipah virus which includes the steps of (a) culturing expression host containing expression vector of recombinant matrix protein in a medium which allows growth of the expression host, (b) allowing the recombinant matrix protein to be expressed in the expression host, (c) harvesting the expression host from step (b) from the medium, (d) disrupting the expression host harvested from step (c) in a medium for sonication purposes and (e) purifying the recombinant matrix protein obtained from step (d). Also provided is a method for purifying recombinant matrix protein of Nipah virus which includes the steps of (a) harvesting and lysing recombinant expression host containing recombinant matrix protein cells, (b) purifying the recombinant matrix protein using nickel affinity column chromatography, (c) dialyzing the purified recombinant matrix protein from step (b) with buffer solution, (d) fractionating the purified recombinant matrix protein from step (c) using centrifugation and (e) collecting and pooling the fractionated recombinant matrix protein obtained from step (d) concentration of protein. There is also provided a pharmaceutical composition comprising a therapeutically effective amount of recombinant matrix protein of Nipah virus. Also provided is a diagnostic reagent comprising an effective amount of recombinant matrix protein matrix protein of Nipah virus.
    • 本发明提供了含有至少一个His标签和myc表位的Nipah病毒基质蛋白的Nipah病毒重组基质蛋白。 此外,本发明还提供了包含Nipah病毒的重组基质蛋白的重组质粒。 还提供了生产Nipah病毒重组基质蛋白的方法,其包括以下步骤:(a)在允许表达宿主生长的培养基中培养包含重组基质蛋白表达载体的表达宿主,(b)使重组基质蛋白 (c)从培养基中收获来自步骤(b)的表达宿主,(d)在培养基中破碎步骤(c)中收获的表达宿主以达到超声处理目的,和(e)纯化重组体 从步骤(d)获得的基质蛋白。 本发明还提供了纯化Nipah病毒重组基质蛋白的方法,其包括以下步骤:(a)收获并裂解含重组基质蛋白细胞的重组表达宿主,(b)使用镍亲和柱层析纯化重组基质蛋白,(c) (d)使用离心分离来自步骤(c)的纯化的重组基质蛋白,和(e)收集并合并步骤(d)获得的分级重组基质蛋白 的蛋白质。 还提供了包含治疗有效量的尼帕病毒重组基质蛋白的药物组合物。 还提供了包含有效量的Nipah病毒的重组基质蛋白基质蛋白的诊断试剂。