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    • 62. 发明公开
    • OIL DEGUMMING METHODS
    • 油脱胶方法
    • EP2488639A1
    • 2012-08-22
    • EP10765555.7
    • 2010-10-08
    • Bunge Oils, Inc
    • DAYTON, Christopher, L., G.GALHARDO, Flavio, Da SilvaBARTON, NelsonHITCHMAN, TimLYON, JonathanO'DONOGHUE, EileenWALL, Mark, A.
    • C12N9/88C11B3/00C11B3/02C12P7/64C12P7/18C10L1/02
    • C11B3/003C10G2300/1011C10L1/026C11B3/04C12N9/88C12P7/18C12P7/6445C12P7/6481C12Y406/01013Y02E50/13Y02P20/52Y02P30/20
    • In alternative embodiments, the invention provides phosphatidylinositol-specific phospholipase C (PI-PLC) enzymes, nucleic acids encoding them, antibodies that bind specifically to them, and methods for making and using them. Industrial methods and products comprising use of these phospholipases are also provided. In certain embodiments, provided herein are methods for hydration of non hydratable phospholipids (NHPs) within a lipid matrix. The methods enable migration of NHPs to an oil-water interface thereby allowing the NHPs to be reacted and/or removed from the lipids. In certain embodiments, provided is a method for removing NHPs, hydratable phospholipids, and lecithins from vegetable oils to produce a degummed oil or fat product that can be used for food production and/or non-food applications. In certain embodiments, provided herein are methods for hydration of NHPs followed by enzymatic treatment and removal of various phospholipids and lecithins. The methods provided herein can be practiced on either crude or water-degummed oils.
    • 在替代实施方案中,本发明提供磷脂酰肌醇特异性磷脂酶C(PI-PLC)酶,编码它们的核酸,特异性结合它们的抗体,以及制备和使用它们的方法。 还提供了包含使用这些磷脂酶的工业方法和产品。 在某些实施方案中,本文提供了用于在脂质基质内水合不可水合磷脂(NHP)的方法。 该方法使得NHPs能够迁移到油 - 水界面,从而允许NHP与脂质反应和/或去除。 在某些实施方案中,提供了从植物油中除去NHP,可水合磷脂和卵磷脂以产生可用于食品生产和/或非食品应用的脱胶油或脂肪产品的方法。 在某些实施方案中,本文提供了用于NHP水合的方法,随后是酶处理和去除各种磷脂和卵磷脂。 本文提供的方法可以在原油或水脱胶油中实施。