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    • 69. 发明申请
    • CONJUGATE HAVING CLEAVABLE LINKING AGENT
    • 具有可清洗连接剂的连接
    • WO2010135330A1
    • 2010-11-25
    • PCT/US2010/035265
    • 2010-05-18
    • ABBOTT LABORATORIESKONRATH, John G.MOORE, Jeffrey A.HIMMELSBACH, Richard J.
    • KONRATH, John G.MOORE, Jeffrey A.HIMMELSBACH, Richard J.
    • G01N33/532G01N33/543G01N33/58
    • G01N33/54333G01N33/582
    • A method and reagent that can be used to eliminate the signal caused by non-specific binding of a labeled conjugate, e.g., a specific binding member attached to a label, to a solid phase, e.g., a magnetic microparticle. The method and the reagent involve the use of a cleavable linking agent to link the label to the specific binding member that specifically binds to the analyte. The use of a cleavable linking agent would allow the release of the label from the specific binding member from the complex comprising the magnetic microparticle, analyte, and labeled conjugate into solution. After the release of the label, the magnetic microparticles having any label non-specifically bound thereto, are removed from the reaction mixture. Only the label, e.g., acridinium, from the labeled conjugate would remain in the elution well. The conjugate that is non-specifically bound through interaction between the label and the solid phase, e.g., a magnetic particle, would remain bound to the solid phase, and would subsequently be removed from the elution well when the solid phase is removed from the elution well and transferred to another well the introduction of additional reagent(s).
    • 可用于消除由标记的缀合物(例如附着于标记物的特异性结合构件)与固相例如磁性微粒的非特异性结合引起的信号的方法和试剂。 该方法和试剂包括使用可切割的连接剂将标记物与特异性结合分析物结合的特异性结合成员连接。 使用可切割交联剂将允许从包含磁性微粒,分析物和标记的缀合物的络合物中将标记从特异性结合成员释放到溶液中。 剥离标签后,从反应混合物中除去具有非特异性结合的标记的磁性微粒。 只有来自标记的缀合物的标记(例如吖啶鎓)将保留在洗脱井中。 通过标记和固相(例如磁性颗粒)之间的相互作用非特异性结合的缀合物将保持与固相结合,并且随后在从洗脱液中除去固相时从洗脱孔中除去 并转移到另一个井,引入额外的试剂。