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51. 发明授权

US3957582A Purification of urokinase 失效
标题翻译：尿激酶的纯化
公开(公告)号：US3957582A
公开(公告)日：1976-05-18
申请号：US525334
申请日：1974-11-20
申请人： Gene Alan Stried , Peter George Sesin
发明人： Gene Alan Stried , Peter George Sesin
IPC分类号： C12N9/72 , C07G7/026
CPC分类号： C12N9/6462 , C12Y304/21073 , Y10S435/815
摘要： This invention relates to the purification of urokinase and more particularly to a method of obtaining urokinase of high potency free of clot-promoting substances such as thromboplastin by treatment of impure aqueous solutions thereof with a modified, cross-linked dextran gel.
摘要翻译：本发明涉及尿激酶的纯化，更具体地说，涉及一种通过用修饰的交联葡聚糖凝胶处理其不纯的水溶液而获得没有凝血促进物质如凝血激酶的高效能的尿激酶的方法。

52. 发明授权

US3930945A Urokinase production 失效
公开(公告)号：US3930945A
公开(公告)日：1976-01-06
申请号：US564966
申请日：1975-03-31
申请人： L. James Lewis
发明人： L. James Lewis
IPC分类号： C12N9/72 , C12K9/00 , C07G7/026
CPC分类号： C12N9/6462 , C12Y304/21073
摘要： The addition of specified amounts of glycine to the production medium for urokinase using live cells increases the production of urokinase by 50 - 600%.

53. 发明授权

US3753860A Preservation of bovine stomachs for rennet extraction 失效
标题翻译：保存用于网络提取的牛血清
公开(公告)号：US3753860A
公开(公告)日：1973-08-21
申请号：US3753860D
申请日：1972-03-02
申请人： PFIZER
发明人： DARDAS R
IPC分类号： C12N9/64 , C07G7/026
CPC分类号： C12N9/6481
摘要： HIGH RECOVERY YIELDS OF RENNER ARE OBTAINED FROM CALF AND ADULT BOVINE STOMACHS PRESERVED BY DIPPING THE STOMACHS IN A 1 TO 10% W./V. PROPYLENE GLYCOL SOLUTION OF 1:1 MIXTURE OF THE METHYL AND PROPYL ESTERS OF PHYDROXYBENZOIC ACID, AND STORING UNDER REFRIGERATION.

54. 发明授权

US3723252A Method for removing pyrogenic substances out of urokinase 失效
标题翻译：从尿素酶中除去有害物质的方法
公开(公告)号：US3723252A
公开(公告)日：1973-03-27
申请号：US3723252D
申请日：1971-08-23
申请人： MOCHIDA PHARM CO LTD
发明人： OGAWA N , KOJI N , MURAYAMA Y
IPC分类号： C12N9/72 , C07G7/026
CPC分类号： C12N9/6462 , C12Y304/21073 , Y10S435/815
摘要： METHOD OF MANUFACTURING UROKINASE FREE FROM PYROGENIC SUBSTANCES BY CONTACTING CRUDE UROKINASE WITH WEAKLY ACIDIC ION EXCHANGER AT AN ELECTROLYTE CONCENTRATION AND PH VALUE AT WHICH SAID UROKINASE IS PREFERENTIALLY ADSORBED BUT PYROGENIC SUBSTANCES ARE NOT.

55. 发明授权

US3650903A Method of production of fibrinolytic material 失效
标题翻译：纤维素材料的生产方法
公开(公告)号：US3650903A
公开(公告)日：1972-03-21
申请号：US3650903D
申请日：1969-07-15
申请人： RAND LAB INC
发明人： SLOANE NATHAN H
IPC分类号： A61K35/22 , C12N9/72 , C07G7/026
CPC分类号： C12N9/6462 , A61K35/22 , C12Y304/21073 , Y10S435/815
摘要： A fibrinolytic material comprising urokinase in highly concentrated form is produced by admixing with human urine an equine adsorbant prepared from an insoluble precipitate which forms in pregnant mare''s urine. This insoluble residue extracts and adsorbs urokinase from human urine thereby permitting its recovery, in concentrated form, in a single step. The urokinase is then eluted from the adsorbant, collected, and purified for use in humans or animals to dissolve blood clots and/or prevent their formation, and for use as a blood thinning agent.
摘要翻译：包含高度浓缩形式的尿激酶的纤维蛋白溶解材料通过与人尿混合，由在孕妇母体尿中形成的不溶性沉淀制备的马吸附剂来制备。这种不溶性残留物从人尿中提取和吸附尿激酶，从而允许其以浓缩形式在单一步骤中恢复。然后从吸附剂中洗脱尿激酶，收集并纯化，以用于人或动物以溶解血块和/或防止其形成，并用作血液稀释剂。

56. 发明授权

US3616231A Process for the production of uricase 失效
标题翻译：生产尿素酶的方法
公开(公告)号：US3616231A
公开(公告)日：1971-10-26
申请号：US3616231D
申请日：1968-11-14
申请人： BOEHRINGER MANNHEIM GMBH
发明人： BERGMEYER HANS ULRICH , MOLLERING HANS
IPC分类号： C12N9/06 , C07G7/026
CPC分类号： C12N9/0046 , Y10S435/815
摘要： Process for isolating uricase from animal tissue such as liver, kidney and spleen comprising extracting the water-insoluble fraction of such a tissue homogenate with an alkaline salt solution, treating the extract containing the uricase with kieselguhr (diatomite), separating the kieselguhr having the uricase adsorbed thereon from the remaining solution, eluting the separated kieselguhr with an alkaline acetone solution, precipitating the uricase in the eluate with ammonium sulfate and purifying the recovered precipitate by washing the same at least once with water or water containing a minor amount of a salt.

57. 发明授权

US4190708A Production of urokinase 失效
标题翻译：生产尿激酶
公开(公告)号：US4190708A
公开(公告)日：1980-02-26
申请号：US909137
申请日：1978-05-24
申请人： Mau-Jung Kuo , Margaret J. Reents , Joseph Feder
发明人： Mau-Jung Kuo , Margaret J. Reents , Joseph Feder
IPC分类号： C12N5/02 , C12N9/72 , C12R1/91 , C07G7/026
CPC分类号： C12N9/6462 , C12Y304/21073
摘要： The yield of urokinase produced by cell culture of kidney cells is improved by incorporating in the cell culture medium an elevated level of phenylalanine.
摘要翻译：通过在细胞培养基中掺入升高水平的苯丙氨酸来改善肾细胞细胞培养产生的尿激酶的产量。

58. 发明授权

US4172763A Preparation of high purity xanthine oxidase from bovine milk 失效
标题翻译：从牛奶制备高纯度黄嘌呤氧化酶
公开(公告)号：US4172763A
公开(公告)日：1979-10-30
申请号：US806736
申请日：1977-06-15
申请人： John P. Zikakis
发明人： John P. Zikakis
IPC分类号： C12N9/04 , C07G7/026
CPC分类号： C12N9/0006 , C12Y117/03002 , Y10S435/803
摘要： Bovine milk xanthine oxidase, referred to herein as XO, is isolated and purified from raw whole milk by a streamline method without the use of proteolytic and lipolytic enzymes, butanol, or other organic solvents. Sodium salicylate, ethylenediaminetetraacetate (EDTA), and a 0.2 M phosphate buffer are added to fresh milk. After incubation at 40.degree.-45.degree. C. for 105 min., the mixture is adjusted to 1-2% with Triton X-100 and allowed to incubate for 15 min. The mixture is cooled to 4.degree. C., followed by a 2-step fractionation of the proteins with ammonium sulfate. The crude enzyme is isolated as a red-brown precipitate which is dissolved in 0.1 M Tris/CaCL.sub.2 buffer and stored for from 12 to 168 hours at -20.degree. C. The isolated enzyme is purified by column chromatography (Sephadex G-75, Sephacryl S-200, Sepharose 6B, and Sephadex G-75). The final stage of purification is accomplished by passing the enzyme preparation through a DEAE-Sephadex A-50 column in a continuous linear salt gradient from 0.005 M to 0.1 M pyrophosphate buffer.The purified enzyme is not denatured and has, on the average, a constant E.sub.280 /E.sub.450 ratio of 4.1, and one symmetric peak by gel chromatography. Analysis by polyacrylamide disc gel electrophoresis demonstrates a single band, while commercially available XO shows 7-14 bands, only one of which contains XO activity (the rest being impurities). The average yield of the final product is about 21% which is 110% higher than the yield of less pure XO obtained using the best prior art.
摘要翻译：牛奶黄嘌呤氧化酶（本文称为XO）通过流线法从原始全脂奶中分离和纯化，而不使用蛋白水解和脂肪分解酶，丁醇或其它有机溶剂。将水杨酸钠，乙二胺四乙酸（EDTA）和0.2M磷酸盐缓冲液加入新鲜的牛奶中。在40°-45℃温育105分钟后，用Triton X-100将混合物调节至1-2％，使其孵育15分钟。将混合物冷却至4℃，随后用硫酸铵分级分离蛋白质。分离粗酶作为红棕色沉淀物，将其溶于0.1M Tris / CaCL 2缓冲液中，在-20℃保存12至168小时。分离的酶通过柱色谱法（Sephadex G-75，Sephacryl S-200，Sepharose 6B和Sephadex G-75）。纯化的最后阶段是通过使酶制剂通过DEAE-Sephadex A-50柱以0.005M至0.1M焦磷酸盐缓冲液的连续线性盐梯度进行。

59. 发明授权

US4161424A Novel endonuclease and process for production thereof 失效
标题翻译：新型内切核酸酶及其制备方法
公开(公告)号：US4161424A
公开(公告)日：1979-07-17
申请号：US816541
申请日：1977-07-18
申请人： Tadahiko Ando , Takehiko Shibata , Shukuko Ikawa , Cholung Kim
发明人： Tadahiko Ando , Takehiko Shibata , Shukuko Ikawa , Cholung Kim
IPC分类号： C12N9/16 , C12N9/22 , C07G7/026 , C12D13/06
CPC分类号： C12N9/22
摘要： The present invention relates to novel enzymes which decompose nucleic acid and are endowed with a substrate specificity recognizing specific biological deoxyribonucleic acid and cleaving specific bonds thereof to produce fragments of deoxyribonucleic acid of specific sizes and which are obtained by culturing microorganisms belonging to genus Bacillus and purifying the cell free extract thereof by such treatment as streptomycin treatment, ammonium sulfate precipitation, ion exchange chromatography, gel filtration or combination of these processes.
摘要翻译：本发明涉及分解核酸并具有识别特异性生物脱氧核糖核酸的底物特异性并切割其特异性键的新型酶，其产生特异性大小的脱氧核糖核酸片段，其通过培养属于芽孢杆菌属的微生物和纯化其通过如链霉素处理，硫酸铵沉淀，离子交换层析，凝胶过滤或这些方法的组合的处理的无细胞提取物。

60. 发明授权

US4137127A Process for the preparation of thrombin-like enzymes from snake venoms 失效
标题翻译：从蛇毒中制备凝血酶样酶的方法
公开(公告)号：US4137127A
公开(公告)日：1979-01-30
申请号：US819473
申请日：1977-07-27
申请人： Kurt F. Stocker
发明人： Kurt F. Stocker
IPC分类号： C12N9/74 , A61K35/58 , A61K38/00 , A61K38/46 , A61K38/48 , C12N9/64 , C12Q1/56 , G01N33/68 , C07G7/026
CPC分类号： C12Q1/56 , C12N9/6418 , A61K38/00
摘要： A process for the preparation of thrombin-like proteolytic enzymes from snake venoms or fractions thereof comprising contacting the snake venom or fraction thereof with heparin insolubilized by reaction with a water-insoluble carrier in order to bind the thrombin-like enzyme to the insolubilized heparin, removing the undesirable accompanying substances of the snake venom and splitting the thrombin-like enzyme from the insolubilized heparin.
摘要翻译：一种从蛇毒或其馏分制备凝血酶样蛋白水解酶的方法，包括使蛇毒或其部分与不溶于水不溶性载体的肝素接触，以将凝血酶样酶与不溶性肝素结合，除去蛇毒的不良伴随物质并从不溶性肝素中分离凝血酶样酶。

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