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    • 53. 发明公开
    • 호열성 및 호산성 아라비노스 이성화효소 및 그를 이용한 타가토스의 제조방법
    • 可疑和酸性阿拉伯糖异构体及其制备标签的方法
    • KR1020040058544A
    • 2004-07-05
    • KR1020020084822
    • 2002-12-27
    • 변유량
    • 이상재이동우김병찬최은아홍영호김성보변유량
    • C12N9/90
    • C12N9/90C12N15/70C12P19/02C12Y503/01003
    • PURPOSE: Thermostable and acidophilic arabinose isomerase and a process for preparing tagatose using the same are provided, which recombinant arabinose isomerase has improved acid and heat resistance, prepares tagatose in higher yield under acid condition, and minimize preparation of by-products when tagatose is prepared under acid condition. CONSTITUTION: The thermostable and acidophilic arabinose isomerase has the following properties: (i) optimal temperature of 50 to 80 deg.C, (ii) optimal pH of 5.0 o 7.0, (iii) molecular weight of 56 kDa, and (iv) 50% or more activity at pH 5, wherein the arabinose isomerase is isolated from Alicyclobacillus acidocaldarius. The process for preparing tagatose comprises the steps of: constructing a vector containing the nucleotide sequence set forth in SEQ ID NO: 1 encoding the arabinose isomerase having the amino acid sequence set forth in SEQ ID NO: 2; transforming a host cell with the vector to produce a transformant; culturing the transformant to produce arabinose isomerase; and contacting the arabinose isomerase with galactose at pH 4.0 to 7.0 and 40 to 85 deg.C.
    • 目的:提供热稳定性和嗜酸性阿拉伯糖异构酶和使用其制备塔格糖的方法,该重组阿拉伯糖异构酶具有改善的耐酸和耐热性,在酸性条件下以更高的产率制备塔格糖,并且在制备塔格糖时最小化副产物的制备 在酸性条件下。 构成:热稳定和耐酸性的阿拉伯糖异构酶具有以下性质:(i)最佳温度为50至80摄氏度,(ii)最佳pH为5.0至7.0,(iii)分子量为56kDa,(iv)为50 在pH5下具有%或更多的活性,其中阿拉伯糖异构酶从酸性脂环酸杆菌中分离出来。 制备塔格糖的方法包括以下步骤:构建含有编码具有SEQ ID NO:2所示氨基酸序列的阿拉伯糖异构酶的SEQ ID NO:1所示的核苷酸序列的载体; 用载体转化宿主细胞以产生转化体; 培养转化体以产生阿拉伯糖异构酶; 并将阿拉伯糖异构酶与pH 4.0至7.0和40至85℃的半乳糖接触。