专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

51. 发明授权

US08648298B2 Excitation of ions in ICR mass spectrometers 有权
标题翻译：在ICR质谱仪中激发离子
公开(公告)号：US08648298B2
公开(公告)日：2014-02-11
申请号：US12633421
申请日：2009-12-08
申请人： Jochen Franzen
发明人： Jochen Franzen
IPC分类号： H01J49/00
CPC分类号： H01J49/38
摘要： In an ion cyclotron resonance mass spectrometer ions are excited into cyclotron orbits by an alternating current excitation signal having a nonlinear function of the excitation frequency vs. time in a “chirp.” Such an excitation signal produces transients which have no pronounced beats, even if mixtures of many ion species, all having the same mass differences, are present. The dynamic measuring range for the image currents can thus be better utilized. In particular, sum spectra of specified quality can be generated from a significantly smaller number of individual transients, and thus in a significantly shorter measuring time.
摘要翻译：在离子回旋共振质谱仪中，通过在“啁啾声”中具有激发频率对时间的非线性函数的交流激励信号将离子激发到回旋加速器轨道。这种激发信号产生没有显着节拍的瞬变，即使存在所有具有相同质量差异的许多离子物质的混合物。因此可以更好地利用图像电流的动态测量范围。特别地，可以从显着较小数量的单个瞬变产生特定质量的和光谱，并且因此在显着较短的测量时间内产生。

52. 发明授权

US08624180B2 Resolution enhancement for ion mobility spectrometers 有权
标题翻译：离子迁移谱仪的分辨率提升
公开(公告)号：US08624180B2
公开(公告)日：2014-01-07
申请号：US13094146
申请日：2011-04-26
申请人： Melvin Andrew Park , Jochen Franzen
发明人： Melvin Andrew Park , Jochen Franzen
IPC分类号： H01J49/00
CPC分类号： G01N27/626
摘要： In an ion mobility spectrometer in which a gas pushes ions along a spectrometer axis against and over an electrical field barrier, the electric field barrier is generated with a plateau of slightly increasing height along the axis of the spectrometer. Alternately, the electric filed barrier may have a plateau with constant height, but the gas flow decreases in velocity along the axis of the spectrometer in the vicinity of the plateau.
摘要翻译：在离子迁移谱仪中，其中气体沿着光谱仪轴线推动离子抵抗和超过电场屏障，电场屏障是沿着光谱仪轴线的高度稍微增加的高原产生的。或者，电场屏障可以具有恒定高度的平台，但气流在平台附近沿着光谱仪的轴线的速度减小。

53. 发明授权

US08605968B2 Determination of tissue states by imaging mass spectrometry 有权
标题翻译：通过成像质谱法测定组织状态
公开(公告)号：US08605968B2
公开(公告)日：2013-12-10
申请号：US13028676
申请日：2011-02-16
申请人： Fedor Alexandrov
发明人： Fedor Alexandrov
IPC分类号： G06K9/00
CPC分类号： H01J49/0036 , G06K9/0014 , G06K9/6209 , G06T5/002 , G06T7/0012 , G06T2207/10056 , G06T2207/20076 , G06T2207/20192 , G06T2207/30024
摘要： Spatially resolved tissue states (status image) are determined from spectrally resolved mass spectra of a tissue section by (a) acquiring a plurality of spatially resolved mass spectra of the tissue section, (b) generating at least two mass images from the spatially resolved mass spectra, (c) smoothing the mass images using an edge-preserving smoothing algorithm and (d) calculating a status image from the smoothed mass images by means of a classification algorithm derived from mathematical statistics.
摘要翻译：空间分辨的组织状态（状态图像）通过（a）获取组织部分的多个空间分辨质谱来确定组织切片的光谱解析质谱图，（b）从空间分辨质量产生至少两个质量图像光谱，（c）使用边缘保持平滑算法平滑质量图像，以及（d）通过从数学统计导出的分类算法从平滑质量图像计算状态图像。

54. 发明授权

US08604426B2 Radical ions for electron transfer dissociation 有权
标题翻译：用于电子转移离解的自由基离子
公开(公告)号：US08604426B2
公开(公告)日：2013-12-10
申请号：US13772701
申请日：2013-02-21
申请人： Bruker Daltonik GmbH
发明人： Ralf Hartmer
IPC分类号： H01J49/10 , G01N33/68 , B01D59/44
CPC分类号： G01N33/6848 , H01J49/0072
摘要： Radical anions for use in the fragmentation of positively charged biopolymer ions by means of electron transfer are produced from substances previously unknown for use as ETD production substances. The inventive substances produce radical anions that lead to electron transfer dissociations with a high yield of fragment ions. The substances have high volatility that allows them to be kept in unheated containers outside the vacuum system and transported into the vacuum system to an in vacuum electron attachment ion source via unheated lines and low molecular weights that allow the measurement of even very light fragment ions. In one embodiment, a suitable substance is 1-3-5-7-cyclooctatetraene.
摘要翻译：用于通过电子转移使带正电荷的生物聚合物离子分解的自由基阴离子由以前未知的用作ETD生产物质的物质产生。本发明的物质产生导致电子转移离解的自由基阴离子，具有高产率的碎片离子。这些物质具有高挥发性，使其能够保持在真空系统外部的未加热的容器中，并通过未加热的管线和低分子量输送到真空电子附着离子源，允许测量甚至非常轻的碎片离子。在一个实施方案中，合适的物质是1-3-5-7-环辛四烯。

55. 发明申请

US20130302847A1 METHOD AND SAMPLE SUPPORT TO ASSIST THE MANUAL PREPARATION OF SAMPLES FOR IONIZATION WITH MATRIX-ASSISTED LASER DESORPTION 审中-公开
标题翻译：方法和样品支持，以协助手工制备用于基质辅助激光解吸的离子
公开(公告)号：US20130302847A1
公开(公告)日：2013-11-14
申请号：US13990582
申请日：2011-11-23
申请人： Guido Mix
发明人： Guido Mix
IPC分类号： G01N1/28
CPC分类号： G01N1/28 , B01J2219/00682 , B01J2219/00684 , B01J2219/00693 , B01J2219/00695 , B01J2219/00698 , B01J2219/00704 , B01L9/523 , B01L9/56 , B01L2200/0621 , B01L2300/02 , B01L2300/0627 , B01L2300/0829 , H01J49/0413 , H01J49/0418
摘要： The invention relates to a method to assist with the manual preparation of a sample support for ionization with matrix-assisted laser desorption where a sample support with sample sites is provided, a selected sample site is highlighted in a way which can be perceived by the human eye at least with respect to neighboring, not selected sample sites, a sample is manually deposited on the selected and highlighted sample site, and the deposition state of at least the selected and highlighted sample site is determined. The method enables the sample preparation and sample analysis to be made more efficient and more certain.
摘要翻译：本发明涉及一种帮助手动制备用基质辅助激光解吸的电离样品支架的方法，其中提供了样品位点的样品支架，选定的样品位点以可被人感知的方式突出显示至少相对于相邻的未选择的样品位置，将样品手动沉积在所选择和突出显示的样品位点上，并且确定至少所选择和突出显示的样品位点的沉积状态。该方法使样品制备和样品分析更有效率和更确定。

56. 发明授权

US08581179B2 Protein sequencing with MALDI mass spectrometry 有权
标题翻译：用MALDI质谱法进行蛋白质测序
公开(公告)号：US08581179B2
公开(公告)日：2013-11-12
申请号：US12708010
申请日：2010-02-18
申请人： Jochen Franzen
发明人： Jochen Franzen
IPC分类号： H01J49/40 , H01J49/04
CPC分类号： H01J49/164 , G01N33/6851 , H01J49/0045
摘要： In a mass spectrometer, sample ions are produced by using matrix assisted laser desorption with a matrix substance that supports spontaneous, non-ergodic ISD fragmentation and a laser light source with nanosecond light pulses and a multiple spot beam profile. A plurality of individual time-of-flight spectra are recorded from the resulting ions in such a way that amplification of ion signals in the mass spectrometer detector is initially reduced so that only ions with masses near a mass range limit are initially recorded. During the repeated acquisitions of the individual time-of-flight spectra, both the detector amplification and the mass range limit are increased. By these methods, it is possible to evaluate c and z fragment ions in lower mass ranges and to directly read N-terminal sequences from near terminus up to 80 amino acids and beyond, and C-terminal sequences up to more than 60 amino acids.
摘要翻译：在质谱仪中，通过使用支持自发的，非遍历的ISD碎片的基质物质和具有纳秒光脉冲的激光光源和多点光束轮廓的矩阵辅助激光解吸来产生样品离子。从所得到的离子记录多个单独的飞行时间频谱，使得质谱仪检测器中的离子信号的放大初始地减少，使得只有最初记录质量接近质量范围极限的离子。在重复采集单个飞行时间光谱期间，检测器放大和质量范围极限都增加。通过这些方法，可以评估较低质量范围内的c和z片段离子，并直接从近端直到80个氨基酸及其以外的N末端序列，以及直到超过60个氨基酸的C-末端序列。

57. 发明授权

US08580535B2 Mass spectrometric measurement of microbial resistances 有权
公开(公告)号：US08580535B2
公开(公告)日：2013-11-12
申请号：US13523167
申请日：2012-06-14
申请人： Vadim Markovich Govorun , Jochen Franzen
发明人： Vadim Markovich Govorun , Jochen Franzen
IPC分类号： C12Q1/04
CPC分类号： G01N33/6851 , C12Q1/18
摘要： Microorganisms, particularly bacteria, are identified and characterized on the basis of a mass spectrometric measurement of their protein profiles with ionization by matrix-assisted laser desorption. In order to measure the microbial resistance to antibiotics, the protein profiles of microorganisms are measured after cultivation for a short time duration in nutrient media containing the antibiotics.

58. 发明授权

US08569010B2 Mass spectrometric diagnosis of septicemia 有权
标题翻译：败血病的质谱诊断
公开(公告)号：US08569010B2
公开(公告)日：2013-10-29
申请号：US13322363
申请日：2010-07-14
申请人： Thomas Maier
发明人： Thomas Maier
IPC分类号： C12Q1/04 , C12Q1/24 , C12Q1/10 , A01N1/02 , C12Q1/00 , C12Q1/02 , C12Q1/14
CPC分类号： C12Q1/24 , C12Q1/04 , G01N33/6851 , G01N2560/00 , G01N2570/00 , G01N2800/26
摘要： The invention mainly relates to the mass spectrometric identification of pathogens in blood cultures from blood-stream infections (septicemia). The invention provides a method with which microbial pathogens can be separated in purified form from blood after a relatively brief cultivation in a blood culture flask, without any interfering human proteins or any residual fractions of blood particles such as erythrocytes and leukocytes, and can be directly identified by mass spectrometric measurement of their protein profiles. The method is based on the use of relatively strong tensides to destroy the blood particles by dissolving the weak cell membranes and most of the internal structures of the blood particles; in spite of the fact that tensides are regarded as strong ionization inhibitors in MALDI and other ionization processes required for mass spectrometric measurements. This method allows unknown pathogens to be obtained in their pure form by centrifuging or filtration and to be identified on the taxonomic level of species or subspecies. Problems with DNA from high levels of leukocytes can be resolved by special measures. After sufficient cultivation, the identification in a mass spectrometric laboratory takes only half an hour.
摘要翻译：本发明主要涉及血流感染（败血病）血液培养物中病原体的质谱鉴定。本发明提供了一种在血培养瓶中比较短暂的培养后，将微生物病原体与血液分离的方法，其中没有任何干扰的人类蛋白质或血液颗粒如红细胞和白细胞的任何残留部分，并且可直接通过其蛋白质谱的质谱测定来鉴定。该方法基于使用相对较强的表面活性剂通过溶解弱细胞膜和血液颗粒的大部分内部结构来破坏血液颗粒; 尽管在MALDI和质谱测量所需的其他电离过程中，表面活性剂被认为是强电离抑制剂。该方法允许通过离心或过滤以其纯形式获得未知的病原体，并根据物种或亚种的分类级别进行鉴定。通过特殊措施可以解决高水平白细胞DNA的问题。在充分培养后，质谱实验室的鉴定只需半小时。

59. 发明授权

US08513599B2 Guiding spray droplets into an inlet capillary of a mass spectrometer 有权
标题翻译：引导喷雾液滴进入质谱仪的入口毛细管
公开(公告)号：US08513599B2
公开(公告)日：2013-08-20
申请号：US12857161
申请日：2010-08-16
申请人： Jochen Franzen , Claus Köster
发明人： Jochen Franzen , Claus Köster
IPC分类号： H01J49/26
CPC分类号： H01J49/062 , H01J49/0445
摘要： Charged droplets are guided along a defined path from a droplet source to a droplet sink. A focusing pseudopotential distribution generated by audio frequencies on electrodes of a guiding device guide the charged droplets from the droplet source to the droplet sink with low loss. The droplets can be driven along the droplet guide by a gas flow, an axial electric field or a combination of both. For example, charged droplets from a spray capillary of an electrospray ion source at atmospheric pressure may be introduced into the inlet capillary leading to the vacuum system of ion analyzers, a procedure similar to that used up to now in nanoelectrospraying, but with substantially higher flow rates. In the guiding device, the droplets can be manipulated in different ways, for example evaporated down to a desired size. The introduction of small droplets into gas-aspirating capillaries is of interest because it is possible to keep the droplets on axis by Bernoulli focusing and to guide them in large quantities and with low loss through the capillary. The ability to guide the droplets makes it also possible to install a segmented inlet capillary with intermediate pumping, which allows pumping capacity to be saved. Advantageously, the sensitivity of ion analyzers such as mass spectrometers or ion mobility spectrometers by at least one order of magnitude.
摘要翻译：带电液滴沿着从液滴源到液滴槽的限定路径被引导。由引导装置的电极上的音频产生的聚焦伪势分布将带电液滴从液滴源引导到具有低损耗的液滴槽。液滴可以通过气流，轴向电场或两者的组合沿液滴引导件驱动。例如，在大气压下来自电喷雾离子源的喷雾毛细管的带电液滴可以引入入口毛细管中，导入离子分析仪的真空系统，该方法与迄今为止在纳电电喷雾中使用的方法类似，但具有显着更高的流动价格。在引导装置中，液滴可以以不同的方式进行操作，例如蒸发到所需尺寸。将小液滴引入吸气毛细管是令人感兴趣的，因为可以通过伯努利聚焦将液滴保持在轴上，并且通过毛细管大量引导并以低损耗引导。引导液滴的能力使得还可以安装具有中间泵送的分段入口毛细管，这允许节省泵送能力。有利地，诸如质谱仪或离子迁移谱仪的离子分析仪的灵敏度至少有一个数量级。

60. 发明授权

US08481928B2 Introduction of ions into mass spectrometers through laval nozzles 有权
标题翻译：离子通过拉瓦尔喷嘴引入质谱仪
公开(公告)号：US08481928B2
公开(公告)日：2013-07-09
申请号：US12862299
申请日：2010-08-24
申请人： Jochen Franzen
发明人： Jochen Franzen
IPC分类号： H01J49/26
CPC分类号： H01J49/0422
摘要： Ions entrained in a gas are transported into the vacuum system of an ion user, such as a mass spectrometer, from an ion source located outside the vacuum. The gas and ions pass through a nozzle that connects the ion source to the vacuum system and is shaped to form a supersonic gas jet in a first vacuum chamber of the vacuum system. In the first vacuum chamber, ions entrained in the supersonic gas jet are extracted electrically or magnetically and are collected, for example, by an RF ion funnel and transmitted to the ion user. The supersonic gas jet travels on and, after passing through the first vacuum chamber, the supersonic gas jet is directed into a separate pump chamber out of which the gas is pumped.
摘要翻译：携带在气体中的离子从位于真空外部的离子源转移到离子用户（例如质谱仪）的真空系统中。气体和离子通过将离子源连接到真空系统的喷嘴，并被成形为在真空系统的第一真空室中形成超音速气体射流。在第一真空室中，夹带在超声波气体射流中的离子被电或磁性地提取，并且例如通过RF离子漏斗收集并传输给离子用户。超音速气体射流在通过第一真空室之后行进，超音速气体射流被引导到单独的泵室中，气体被泵送。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式