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    • 55. 发明授权
    • Methods of conferring ppo-inhibiting herbicide resistance to plants by gene manipulation
    • 通过基因操作赋予ppo抑制除草剂对植物抗性的方法
    • US07586023B1
    • 2009-09-08
    • US09331723
    • 1996-12-27
    • John E. BoyntonNicholas W. GillhamBarbara L. Randolph-AndersonFumiharu IshigeRyo Sato
    • John E. BoyntonNicholas W. GillhamBarbara L. Randolph-AndersonFumiharu IshigeRyo Sato
    • C12N5/10C12N15/82C07H21/04
    • C12N9/001C12N15/8274C12Q1/18
    • The present invention provides methods to confer resistance to protoporphyrinogen-inhibiting herbicides onto crop plants. Resistance is conferred by genetically engineering the plants to express cloned DNA encoding a protoporphyrinogen oxidase resistant to porphyric herbicides. If such resistant crop plants are cultivated, utilization of these herbicides on fields of these crop plants becomes feasible. This should allow for simpler and more effective weed management, and increase the value of these herbicides for agricultural use. Furthermore, the present invention provides plants, algae, plant cells, and algal cells which have been made resistant to protoporphyrinogen oxidase-inhibiting herbicides by the subject methods using a herbicide-resistant protoporphyrinogen oxidase gene that has been prepared by genetic engineering methods. In addition, the present invention provides methods to evaluate the inhibitory effects of test compounds on protoporphyrinogen oxidase activity, as well as methods to identify protoporphyrinogen oxidase inhibitors among test compounds. Preferred cloned DNA fragments encoding protoporphyrinogen oxidase enzymes resistant to porphyric herbicides are also described.
    • 本发明提供了赋予作物植物抗原卟啉原抑制性除草剂抗性的方法。 通过遗传工程植物表达抗性,以表达编码对卟啉除草剂有抗性的原卟啉原氧化酶的克隆DNA。 如果栽培这种抗性作物,这些除草剂在这些作物田间的利用变得可行。 这样可以使更简单,更有效的杂草管理,并增加这些用于农业的除草剂的价值。 此外,本发明通过使用已经通过遗传工程方法制备的除草剂抗原原卟啉原氧化酶基因的本发明方法提供已经对原卟啉原氧化酶抑制性除草剂具有抗性的植物,藻类,植物细胞和藻细胞。 此外,本发明提供了评估测试化合物对原卟啉原氧化酶活性的抑制作用的方法以及在测试化合物中鉴定原卟啉原氧化酶抑制剂的方法。 还描述了编码对卟啉除草剂有抗性的原卟啉原氧化酶的优选的克隆DNA片段。