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51. 发明专利

CA2425956C NOVEL GLYPHOSATE N-ACETYLTRANSFERASE (GAT) GENES 未知
公开(公告)号：CA2425956C
公开(公告)日：2014-12-23
申请号：CA2425956
申请日：2001-10-29
申请人： MAXYGEN INC , PIONEER HI BRED INT , DU PONT
发明人： CASTLE LINDA A , SIEHL DAN , GIVER LORRAINE J , MINSHULL JEREMY , IVY CHRISTINA , CHEN YONG HONG , DUCK NICHOLAS B , MCCUTCHEN BILLY F , KEMBLE ROGER , PATTEN PHILLIP A
IPC分类号： A01H5/00 , C12N15/54 , C12N5/10 , C12N9/10 , C12N15/09 , C12N15/82
摘要： Novel proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are novel polynucleotides capable of encoding these proteins, compositions that include one or more of these novel proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these novel compounds, diversification methods involving the novel compounds, and methods of using the compounds. Some of the novel methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.

52. 发明专利

CA2832069A1 AGROBACTERIUM-MEDIATED TRANSFORMATION OF DICOT PLANTS 未知
公开(公告)号：CA2832069A1
公开(公告)日：2012-10-11
申请号：CA2832069
申请日：2012-04-03
申请人： PIONEER HI BRED INT , DU PONT
发明人： CHO HYEON-JE , CASTLE LINDA A , KLEIN THEODORE MITCHELL , MINH TAI , MOY YORK
IPC分类号： C12N15/87 , A01H4/00 , A01H5/00 , C12N1/20 , C12N5/04 , C12N5/10 , C12N15/82
摘要： Compositions and methods are provided for the efficient transformation of a dicot plant. More particularly, compositions and methods of the present invention find use in agriculture for Agrobacterium-mediated transformation of a dicotyledonous plant. The compositions include cultivation media comprising high concentrations of sucrose and glucose. The cultivation media find use in methods directed to Agrobacterium-mediated transformation of a dicot plant with a gene of interest. In this manner, any gene of interest can be introduced into a dicot plant with high transformation efficiency and reduced tissue necrosis.

53. 发明专利

CA2805937A1 CHIMERIC PROMOTERS AND METHODS OF USE 未知
公开(公告)号：CA2805937A1
公开(公告)日：2012-02-16
申请号：CA2805937
申请日：2011-08-12
申请人： PIONEER HI BRED INT , DU PONT
发明人： TAO YUMIN , ALBERT HENRIK , CASTLE LINDA A , LU JIAN
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10 , C12N9/02 , C12N15/29
摘要： Compositions and methods comprising promoters from the 4- hydroxyphenylpyruvate dioxygenase (HPPD) gene and active variants and fragments thereof, as well as chimeric promoters employing regulatory regions of the HPPD promoters are provided. Further provided are expression cassettes and plants comprising the various promoters disclosed herein operably linked to a polynucleotide of interest. Methods employing the various promoters described herein to modulate the expression of polynucleotides of interest are further provided.

54. 发明专利

AU2006283504B2 Compositions providing tolerance to multiple herbicides and methods of use thereof 未知
公开(公告)号：AU2006283504B2
公开(公告)日：2011-08-25
申请号：AU2006283504
申请日：2006-08-22
申请人： DU PONT , PIONEER HI BRED INT
发明人： HEGSTAD JEFFREY M , HECKERT MATTHEW J , SAUNDERS DAVID W , CLAUS JON S , GUIDA JR ANTHONY D , YORK MOY , MEHRE WAYNE J , OLSON PAUL D , VOGT MARK D , LU ALBERT L , WONG JAMES F H , WILKINSON JACK Q , HYEON-JE CHO , CASTLE LINDA A , HAZEL CHRISTINE B , CHICOINE TIMOTHY K , FRED MCCUTCHEN BILLY , GREEN JERRY M , DONGLONG LIU , PEEPLES KENNETH A , HUTCHISON JAMES M
IPC分类号： C12N15/82 , A63B20060101 , A63B69/00 , A63B69/36 , A63B71/06
摘要： Methods and compositions are provided related to improved plants that are tolerant to more than one herbicide. Particularly, the invention provides plants that are tolerant of glyphosate and are tolerant to at least one ALS inhibitor, and methods of use thereof. The glyphosate/ALS inhibitor-tolerant plants comprise a polynucleotide that encodes a polypeptide that confers tolerance to glyphosate and a polynucleotide that encodes an ALS inhibitor-tolerant polypeptide. In specific embodiments, a plant of the invention expresses a GAT polypeptide and an HRA polypeptide. Methods to control weeds, improve plant yield, and increase transformation efficiencies are provided.

55. 发明专利

BRPI0615088A2 未知
公开(公告)号：BRPI0615088A2
公开(公告)日：2009-07-14
申请号：BRPI0615088
申请日：2006-08-22
申请人： PIONEER HI BRED INT , DU PONT
发明人： MCCUTCHEN BILLY FRED , CASTLE LINDA A , CHICOINE TIMOTHY K , CHO HYEON-JE , CLAUS JON S , GREEN JERRY M , GUIDA ANTHONY D JR , HAZEL CHRISTINE B , HECKERT MATTHEW J , HEGSTAD JEFFREY M , HUTCHISON JAMES M , LIU DONGLONG , LU ALBERT L , MEHRE WAYNE J , MOY YORK , OLSON PAUL D , PEEPLES KENNETH A , SAUNDERS DAVID W , VOGT MARK D , WILKINSON JACK Q , WONG JAMES F H
IPC分类号： C12N15/82 , A63B20060101 , A63B69/00 , A63B69/36 , A63B71/06
摘要： Methods and compositions are provided related to improved plants that are tolerant to more than one herbicide. Particularly, the invention provides plants that are tolerant of glyphosate and are tolerant to at least one ALS inhibitor, and methods of use thereof. The glyphosate/ALS inhibitor-tolerant plants comprise a polynucleotide that encodes a polypeptide that confers tolerance to glyphosate and a polynucleotide that encodes an ALS inhibitor-tolerant polypeptide. In specific embodiments, a plant of the invention expresses a GAT polypeptide and an HRA polypeptide. Methods to control weeds, improve plant yield, and increase transformation efficiencies are provided.

56. 发明专利

NZ526148A Novel glyphosate N-acetyltransferase (GAT) genes 未知
公开(公告)号：NZ526148A
公开(公告)日：2005-09-30
申请号：NZ52614801
申请日：2001-10-29
申请人： PIONEER HI BRED INT
发明人： CASTLE LINDA A , SIEHL DAN , GIVER LORRAINE J , MINSHULL JEREMY , IVY CHRISTINA , CHEN YONG HONG , DUCK NICHOLAS B , MCCUTCHEN BILLY F , KEMBLE ROGER , PATTEN PHILLIP A
IPC分类号： A01H5/00 , C12N5/10 , C12N9/10 , C12N15/09 , C12N15/54 , C12N15/82
摘要： An isolated or recombinant polynucleotide encodes a polypeptide that has glycophosate N-acetyl transferase activity and preferably comprises a nucleotide sequence encoding an amino acid sequence consisting of SEQ. ID No.: 300, SEQ. ID No.: 445, or SEQ. ID. No.:457. Methods of producing the polypeptide, or a glyphosate resistant transgenic plant, seed or plant cell comprise (a) transforming a plant or plant cell with a polynucleotide encoding a glyphosate N-acetyltransferase and (b) optionally regenerating a transgenic plant from the transformed plant cell. Methods for selectively controlling weeds in a field containing a crop comprise: (a) planting the field with crop seeds or plants which are glyphosate-tolerant as a result of being transformed with a polynucleotide encoding a glyphosate N-acetyltransferase and (b) applying to the crop and weeds in the field a sufficient amount of glyphosate to control the weeds without significantly affecting the crop. Cells containing the polynucleotides and a transgenic plant, seed or transgenic plant explant that exhibits enhanced resistance to glyphosate as compared to a wild type plant of the same species, strain or cultivar are also described.

57. 发明专利

CA2894213A1 COMPOSITIONS AND METHODS FOR AUXIN-ANALOG CONJUGATION 未知
公开(公告)号：CA2894213A1
公开(公告)日：2014-06-26
申请号：CA2894213
申请日：2013-12-20
申请人： PIONEER HI BRED INT
发明人： CASTLE LINDA A , CHIU LI-WEI , TAO YUMIN
IPC分类号： C12N15/82
摘要： Compositions and methods are provided to detoxify an auxin-analog herbicide through the use of at least one GH3 polypeptide having amino acid/auxin analog herbicide conjugation activity. Such GH3 polypeptides in the presence of an auxin-analog herbicide will produce an amino acid/auxin-analog conjugate having reduced herbicidal activity. Various methods of employing the GH3 polypeptides and the polynucleotides encoding the same are provided. Such methods include methods to detoxify an auxin-analog herbicide comprising applying to a plant, a plant cell or a seed an auxin-analog herbicide, wherein the plant, plant cell or seed comprises a heterologous polynucleotide encoding a GH3 polypeptide, and expression of the GH3 polypeptide produces a non-herbicidal aspartate/auxin-analog conjugate or a glutamate/auxin-analog conjugate. Additional methods for controlling at least one weed in an area of cultivation comprising a crop or a seed of the crop are provided, as are methods of detoxifying a contaminated material having an auxin-analog herbicide.

58. 发明专利

AU2011289286A1 Methods and compositions for targeting sequences of interest to the chloroplast 未知
公开(公告)号：AU2011289286A1
公开(公告)日：2013-01-31
申请号：AU2011289286
申请日：2011-08-12
申请人： PIONEER HI BRED INT
发明人： ALBERT HENRIK , CASTLE LINDA A , HECKERT MATTHEW J , LU JIAN , SIEHL DANIEL L , TAO YUMIN
IPC分类号： C12N9/02 , A01H5/00 , A01H5/10 , C12N5/10 , C12N15/29 , C12N15/82
摘要： Chimeric polynucleotides comprising a nucleotide sequence encoding a chloroplast transit peptide operably linked to a heterologous polynucleotide of interest are provided, wherein the chloroplast transit peptide comprises an amino acid sequence having the chloroplast transit peptide sequence as set forth in SEQ ID NO: 1 or a biologically active variant or fragment thereof or wherein the chloroplast transit peptide comprises the sequence set forth in SEQ ID NO: 58 or an active variant or fragment thereof. Chimeric polypeptides encoding the same, as well as, cells, plant cells, plants and seeds are further provided which comprise the chimeric polynucleotides. Compositions further include HPPD polypeptides and polynucleotides encoding the same as set forth in SEQ ID NOS: 57 and 60 or active variants and fragments thereof. Such sequences comprise the chloroplast transit peptide as set forth in SEQ ID NO: 58 or an active variants or fragments thereof. Cells, plant cells, plants and seeds are further provided which comprise such sequences. Methods of use of the various sequences are also provided.

59. 发明专利

AU2011289283A1 Chimeric promoters and methods of use 未知
公开(公告)号：AU2011289283A1
公开(公告)日：2013-01-31
申请号：AU2011289283
申请日：2011-08-12
申请人： PIONEER HI BRED INT , DU PONT
发明人： TAO YUMIN , ALBERT HENRIK , CASTLE LINDA A , LU JIAN
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10 , C12N9/02 , C12N15/29
摘要： Compositions and methods comprising promoters from the 4- hydroxyphenylpyruvate dioxygenase (HPPD) gene and active variants and fragments thereof, as well as chimeric promoters employing regulatory regions of the HPPD promoters are provided. Further provided are expression cassettes and plants comprising the various promoters disclosed herein operably linked to a polynucleotide of interest. Methods employing the various promoters described herein to modulate the expression of polynucleotides of interest are further provided.

60. 发明专利

CA2625371C COMPOSITIONS PROVIDING TOLERANCE TO MULTIPLE HERBICIDES AND METHODS OF USE THEREOF 未知
公开(公告)号：CA2625371C
公开(公告)日：2012-06-26
申请号：CA2625371
申请日：2006-08-22
申请人： PIONEER HI BRED INT , DU PONT
发明人： MCCUTCHEN BILLY FRED , CASTLE LINDA A , CHICOINE TIMOTHY K , CHO HYEON-JE , CLAUS JON S , GREEN JERRY M , GUIDA ANTHONY D JR , HAZEL CHRISTINE B , HECKERT MATTHEW J , HEGSTAD JEFFREY M , HUTCHISON JAMES M , LIU DONGLONG , LU ALBERT L , MEHRE WAYNE J , MOY YORK , OLSON PAUL D , PEEPLES KENNETH A , SAUNDERS DAVID W , VOGT MARK D , WILKINSON JACK Q , WONG JAMES F H
IPC分类号： C12N15/82 , A63B20060101 , A63B69/00 , A63B69/36 , A63B71/06
摘要： Methods and compositions are provided related to improved plants that are tolerant to more than one herbicide. Particularly, the invention provides plants that are tolerant of glyphosate and are tolerant to at least one ALS inhibitor, and methods of use thereof. The glyphosate/ALS inhibitor-tolerant plants comprise a polynucleotide that encodes a polypeptide that confers tolerance to glyphosate and a polynucleotide that encodes an ALS inhibitor-tolerant polypeptide. In specific embodiments, a plant of the invention expresses a GAT polypeptide and an HRA polypeptide. Methods to control weeds, improve plant yield, and increase transformation efficiencies are provided.

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