专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

51. 发明授权

US07951601B2 Oxide layers on silicon substrates for effective confocal laser microscopy 有权
标题翻译：硅衬底上的氧化物层用于有效的共焦激光显微镜
公开(公告)号：US07951601B2
公开(公告)日：2011-05-31
申请号：US11614896
申请日：2006-12-21
申请人： Robert G. Kuimelis , Zihui Chen , Glenn H. McGall
发明人： Robert G. Kuimelis , Zihui Chen , Glenn H. McGall
IPC分类号： G01N33/00 , G01N33/48
CPC分类号： G01N21/6452 , G01N21/6428 , G01N21/6458 , Y10T436/14 , Y10T436/142222 , Y10T436/143333 , Y10T436/25
摘要： Methods of performing confocal laser microscopy on a polymer array disposed on a silicon wafer substrate, the method comprising the steps of providing a silicon wafer substrate having a top side and a bottom side, coating the top side of the silicon wafer with an oxide coating to provide an oxide coated wafer, covalently coupling a plurality of probes to the top side of the coated wafer to provide a fixed polymer array, hybridizing the fixed polymer array with a plurality of labeled ligands, and assaying for one or more hybridized ligands using confocal laser fluorescence microscopy to detect hybridization are provided.
摘要翻译：在设置在硅晶片基板上的聚合物阵列上进行共焦激光显微镜的方法，所述方法包括以下步骤：提供具有顶侧和底侧的硅晶片衬底，用氧化物涂层涂覆硅晶片的顶侧提供氧化物涂覆的晶片，将多个探针共价耦合到涂覆的晶片的顶侧，以提供固定的聚合物阵列，将固定的聚合物阵列与多个标记的配体杂交，以及使用共焦激光测定一个或多个杂交配体提供荧光显微镜检测杂交。

52. 发明申请

US20110046344A1 PARALLEL PREPARATION OF HIGH FIDELITY PROBES IN AN ARRAY FORMAT 有权
标题翻译：并行准备阵列格式中的高密度探测器
公开(公告)号：US20110046344A1
公开(公告)日：2011-02-24
申请号：US12938262
申请日：2010-11-02
申请人： Robert G. Kuimelis , Glenn H. McGall
发明人： Robert G. Kuimelis , Glenn H. McGall
IPC分类号： C07K2/00 , C07H21/04
CPC分类号： B01J19/0046 , B01J2219/00427 , B01J2219/00527 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C40B40/06 , C40B50/14 , C40B80/00
摘要： The present invention provides massively parallel oligonucleotide synthesis and purification for applications that utilize large collections of defined high-fidelity oligonucleotides (e.g., from about 101 to about 105 different sequences, generally between 25-160 bases in length).
摘要翻译：本发明提供大量平行的寡核苷酸合成和纯化，用于利用大量定义的高保真寡核苷酸（例如约101至约105个不同序列，通常在25-160个碱基之间）的应用。

53. 发明申请

US20100305006A1 Parallel Preparation of High Fidelity Probes in an Array Format 有权
标题翻译：以阵列格式并行准备高保真探测器
公开(公告)号：US20100305006A1
公开(公告)日：2010-12-02
申请号：US12855450
申请日：2010-08-12
申请人： Robert G. Kuimelis , Glenn H. McGall
发明人： Robert G. Kuimelis , Glenn H. McGall
IPC分类号： C40B50/18
CPC分类号： B01J19/0046 , B01J2219/00427 , B01J2219/00527 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C40B40/06 , C40B50/14 , C40B80/00
摘要： The present invention provides massively parallel oligonucleotide synthesis and purification for applications that utilize large collections of defined high-fidelity oligonucleotides (e.g., from about 101 to about 105 different sequences, generally between 25-160 bases in length).
摘要翻译：本发明提供大量平行的寡核苷酸合成和纯化，用于利用大量定义的高保真寡核苷酸（例如约101至约105个不同序列，通常在25-160个碱基之间）的应用。

54. 发明申请

US20100298171A1 APPARATUS FOR POLYMER SYNTHESIS 审中-公开
标题翻译：聚合物合成设备
公开(公告)号：US20100298171A1
公开(公告)日：2010-11-25
申请号：US12786287
申请日：2010-05-24
申请人： Mohsen Shirazi , Adam Pawloski , Peter Meijles , Glenn H. McGall
发明人： Mohsen Shirazi , Adam Pawloski , Peter Meijles , Glenn H. McGall
IPC分类号： C40B50/18 , C40B60/14
CPC分类号： C40B40/04 , B01J19/0046 , B01J2219/00286 , B01J2219/00353 , B01J2219/00389 , B01J2219/00432 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00725 , C12Q1/6834 , C40B50/14 , C40B60/14
摘要： Novel processes are disclosed for the large scale preparation of arrays of polymer sequences wherein each array includes a plurality of different, positionally distinct polymer sequences having known monomer sequences. In one embodiment, two substrates are processed simultaneously in a reaction chamber, wherein the substrates are facing each other and in contact with a monomer solution. In a further embodiment, multiple rotating flow cells are used in combination with a photolysis equipment to synthesize wafers.
摘要翻译：公开了用于大规模制备聚合物序列阵列的新方法，其中每个阵列包括具有已知单体序列的多个不同的位置不同的聚合物序列。在一个实施方案中，在反应室中同时处理两个基材，其中基材彼此面对并与单体溶液接触。在另一实施例中，多个旋转流动池与光解设备结合使用以合成晶片。

55. 发明申请

US20090215652A1 Silane Mixtures 有权
标题翻译：硅烷混合物
公开(公告)号：US20090215652A1
公开(公告)日：2009-08-27
申请号：US12358472
申请日：2009-01-23
申请人： Glenn H. McGall , Robert G. Kuimemlis
发明人： Glenn H. McGall , Robert G. Kuimemlis
IPC分类号： C40B50/18
CPC分类号： B01J19/0046 , B01J2219/00432 , B01J2219/00434 , B01J2219/00439 , B01J2219/00497 , B01J2219/00576 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00675 , B01J2219/00677 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B82Y30/00 , C08G77/16 , C08G77/26 , C09D183/06 , C09D183/08 , C40B50/18 , C08L83/00
摘要： Silanation compositions containing a mixture of two or more silanation reagents, where at least one silanation reagent includes a functional group capable of supporting polymer synthesis and at least one silanation reagent includes no functional group capable of supporting polymer synthesis are useful in modulating the active site density and hydrolytic stability of a surface. These compositions are particularly useful in silanating a surface prior to preparation of a polymer array and provide for increased hybridization results.
摘要翻译：含有两种或更多种硅烷化试剂的混合物的硅烷化组合物，其中至少一种硅烷化试剂包括能够支持聚合物合成的官能团和至少一种硅烷化试剂，不包括能够支持聚合物合成的官能团，可用于调节活性部位密度和表面的水解稳定性。这些组合物在制备聚合物阵列之前特别可用于将表面硅烷化，并提供增加的杂交结果。

56. 发明申请

US20090054258A1 Nucleic Acid Labeling Methods 有权
标题翻译：核酸标记方法
公开(公告)号：US20090054258A1
公开(公告)日：2009-02-26
申请号：US12256256
申请日：2008-10-22
申请人： Kyle B. Cole , Vivi Truong , Glenn H. McGall , Anthony D. Barone
发明人： Kyle B. Cole , Vivi Truong , Glenn H. McGall , Anthony D. Barone
IPC分类号： C40B30/04 , C07H19/20 , C07H19/10
CPC分类号： C07H19/20 , C07H19/10 , C07H21/00
摘要： In one aspect of the invention, a method is provided for end-labeling RNA (total RNA, mRNA, cRNA or fragmented RNA). In one aspect of the present invention, T4 RNA ligase is used to attach a 3′-labeled AMP or CMP donor to an RNA acceptor molecule. In another embodiment, a pyrophosphate molecule 3′-AppN-3′-linker-detectable moiety is used as donor molecule.In another aspect of the present invention, a method of detecting the presence of an RNA of interest in a sample is provided, the method having the following steps: providing the sample comprising RNA which may or may not have said RNA of interest; treating the sample with a fragmenting reagent to provide RNA fragments; removing phosphate groups from said fragments to provide fragments with free 3′ OH groups; ligating said fragment with a labeling reagent according to the instant invention; providing a nucleic acid array having probes directed to said RNA of interest; hybridizing the labeled nucleic acid fragments to said nucleic acid array; and determining the extent of hybridization to said probes to determine the presence of said RNA of interest.
摘要翻译：在本发明的一个方面，提供了用于末端标记RNA（总RNA，mRNA，cRNA或片段化RNA）的方法。在本发明的一个方面，T4 RNA连接酶用于将3'标记的AMP或CMP供体连接到RNA受体分子上。在另一个实施方案中，将焦磷酸盐分子3'- AppN-3'-接头可检测部分用作供体分子。在本发明的另一方面，提供了检测样品中目的RNA存在的方法，该方法具有以下步骤：提供包含可能具有或不具有所述目的RNA的RNA的样品; 用碎裂试剂处理样品以提供RNA片段; 从所述片段中除去磷酸基以提供具有游离的3'OH基团的片段; 将所述片段与根据本发明的标记试剂连接; 提供具有针对所述感兴趣的RNA的探针的核酸阵列; 将标记的核酸片段与所述核酸阵列杂交; 以及确定与所述探针的杂交程度，以确定所述目的RNA的存在。

57. 发明授权

US07176297B2 Photoactivatable silane compounds and methods for their synthesis and use 有权
标题翻译：可光活化硅烷化合物及其合成和使用方法
公开(公告)号：US07176297B2
公开(公告)日：2007-02-13
申请号：US10880105
申请日：2004-06-28
申请人： Handong Li , Glenn H. McGall
发明人： Handong Li , Glenn H. McGall
IPC分类号： C07H21/00 , C07H21/02 , C07H21/04 , C12Q1/68
CPC分类号： C40B40/06 , B01J19/0046 , B01J2219/00378 , B01J2219/00608 , B01J2219/00612 , B01J2219/00628 , B01J2219/00637 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C07B2200/11 , C07H21/00 , C40B50/18 , C40B60/14 , G03F7/165 , Y10T428/31663
摘要： In one aspect of the invention, photoactivatable silane compounds and methods for their synthesis and use are provided. In one embodiment, the photoactivatable silane compounds synthesized are represented by a formula: PG-LS-SN, wherein PG is a photoactivatable group, LS is a linkage and spacer group, and SN is a silane group. The silanes allow the photoactivatable silane compounds to be covalently bound to the surface of a substrate such as silica. The photoactivatable group forms a hydrophobic layer that can be photochemically cross-linked with a layer of hydrophilic functional polymers. In another embodiment, a method is disclosed to synthesize a substrate of hydrophobic layers and hydrophilic functional polymer layers thereafter onto glass surfaces. An array of biopolymers such as nucleic acids and peptides may then be covalently attached to the substrate using photolithography and biopolymer synthesis.
摘要翻译：在本发明的一个方面，提供了可光活化的硅烷化合物及其合成和使用的方法。在一个实施方案中，合成的可光活化硅烷化合物由下式表示：PG-LS-SN，其中PG是可光活化基团，LS是连接基和间隔基，SN是硅烷基。硅烷使可光活化的硅烷化合物共价结合到诸如二氧化硅的底物表面。可光活化基团形成疏水层，其可以与一层亲水性官能聚合物光化学交联。在另一个实施方案中，公开了合成疏水层的底物和亲水性功能聚合物层之后的玻璃表面的方法。然后使用光刻和生物聚合物合成将一系列生物聚合物如核酸和肽共价连接到基底上。

58. 发明授权

US06858711B2 Labeling reagents 有权
标题翻译：标记试剂
公开(公告)号：US06858711B2
公开(公告)日：2005-02-22
申请号：US09880727
申请日：2001-06-13
申请人： Glenn H. McGall , Anthony D. Barone
发明人： Glenn H. McGall , Anthony D. Barone
IPC分类号： C07B61/00 , C07H19/052 , C07H19/12 , C07H21/00 , C12Q1/00 , C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C07H19/052 , C07H19/12 , C07H21/00 , C12Q1/6809 , C12Q1/6837 , C12Q2600/156 , C40B40/00 , G06F19/20 , G06F19/22 , C12Q2525/161 , C12Q2565/501 , C12Q2561/125
摘要： The present invention provides a simplified method for identifying differences in nucleic acid abundances (e.g., expression levels) between two or more samples. The methods involve providing an array containing a large number (e.g. greater than 1,000) of arbitrarily selected different oligonucleotide probes where the sequence and location of each different probe is known. Nucleic acid samples (e.g. mRNA) from two or more samples are hybridized to the probe arrays and the pattern of hybridization is detected. Differences in the hybridization patterns between the samples indicates differences in expression of various genes between those samples. This invention also provides a method of end-labeling a nucleic acid. In one embodiment, the method involves providing a nucleic acid, providing a labeled oligonucleotide and then enzymatically ligating the oligonucleotide to the nucleic acid. Thus, for example, where the nucleic acid is an RNA, a labeled oligoribonucleotide can be ligated using an RNA ligase. In another embodiment, the end labeling can be accomplished by providing a nucleic acid, providing labeled nucleoside triphosphates, and attaching the nucleoside triphosphates to the nucleic acid using a terminal transferase.
摘要翻译：本发明提供用于鉴定两个或多个样品之间的核酸丰度差异（例如，表达水平）的简化方法。所述方法包括提供包含大量（例如大于1,000个）任意选择的不同寡核苷酸探针的阵列，其中每个不同探针的序列和位置是已知的。来自两个或更多个样品的核酸样品（例如mRNA）与探针阵列杂交，并检测杂交模式。样本之间的杂交模式的差异表明这些样品之间各种基因的表达差异。本发明还提供了一种终止标记核酸的方法。在一个实施方案中，该方法包括提供核酸，提供标记的寡核苷酸，然后将寡核苷酸酶连接到核酸上。因此，例如，当核酸是RNA时，可以使用RNA连接酶连接标记的寡核糖核苷酸。在另一个实施方案中，末端标记可以通过提供核酸，提供标记的核苷三磷酸和使用末端转移酶将核苷三磷酸与核酸连接来实现。

59. 发明申请

US20120264644A1 Oxide Layers on Silicone Substrates for Effective Confocal Laser Microscopy 审中-公开
标题翻译：氧化层在有机硅基底上有效共焦激光显微镜
公开(公告)号：US20120264644A1
公开(公告)日：2012-10-18
申请号：US13533594
申请日：2012-06-26
申请人： Robert G. Kuimelis , Zihui Chen , Glenn H. McGall
发明人： Robert G. Kuimelis , Zihui Chen , Glenn H. McGall
IPC分类号： C40B30/04
CPC分类号： G01N21/6452 , G01N21/6428 , G01N21/6458 , Y10T436/14 , Y10T436/142222 , Y10T436/143333 , Y10T436/25
摘要： Methods of performing confocal laser microscopy on a polymer array disposed on a silicon wafer substrate, the method comprising the steps of providing a silicon wafer substrate having a top side and a bottom side, coating the top side of the silicon wafer with an oxide coating to provide an oxide coated wafer, covalently coupling a plurality of probes to the top side of the coated wafer to provide a fixed polymer array, hybridizing the fixed polymer array with a plurality of labeled ligands, and assaying for one or more hybridized ligands using confocal laser fluorescence microscopy to detect hybridization are provided.
摘要翻译：在设置在硅晶片基板上的聚合物阵列上进行共焦激光显微镜的方法，所述方法包括以下步骤：提供具有顶侧和底侧的硅晶片衬底，用氧化物涂层涂覆硅晶片的顶侧提供氧化物涂覆的晶片，将多个探针共价耦合到涂覆的晶片的顶侧，以提供固定的聚合物阵列，将固定的聚合物阵列与多个标记的配体杂交，以及使用共焦激光测定一个或多个杂交配体提供荧光显微镜检测杂交。

60. 发明授权

US08227253B2 Oxide layers on silicon substrates for effective confocal laser microscopy 有权
标题翻译：硅衬底上的氧化物层用于有效的共焦激光显微镜
公开(公告)号：US08227253B2
公开(公告)日：2012-07-24
申请号：US13015041
申请日：2011-01-27
申请人： Robert G. Kuimelis , Zihui Chen , Glenn H. McGall
发明人： Robert G. Kuimelis , Zihui Chen , Glenn H. McGall
IPC分类号： C40B40/06 , C40B50/18
CPC分类号： G01N21/6452 , G01N21/6428 , G01N21/6458 , Y10T436/14 , Y10T436/142222 , Y10T436/143333 , Y10T436/25
摘要： Methods of performing confocal laser microscopy on a polymer array disposed on a silicon wafer substrate, the method comprising the steps of providing a silicon wafer substrate having a top side and a bottom side, coating the top side of the silicon wafer with an oxide coating to provide an oxide coated wafer, covalently coupling a plurality of probes to the top side of the coated wafer to provide a fixed polymer array, hybridizing the fixed polymer array with a plurality of labeled ligands, and assaying for one or more hybridized ligands using confocal laser fluorescence microscopy to detect hybridization are provided.
摘要翻译：在设置在硅晶片基板上的聚合物阵列上进行共焦激光显微镜的方法，所述方法包括以下步骤：提供具有顶侧和底侧的硅晶片衬底，用氧化物涂层涂覆硅晶片的顶侧提供氧化物涂覆的晶片，将多个探针共价耦合到涂覆的晶片的顶侧，以提供固定的聚合物阵列，将固定的聚合物阵列与多个标记的配体杂交，以及使用共焦激光测定一个或多个杂交配体提供荧光显微镜检测杂交。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式