专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

51. 发明公开

EP1711829A2 METHODS FOR USING RAMAN SPECTROSCOPY TO OBTAIN A PROTEIN PROFILE OF A BIOLOGICAL SAMPLE 审中-公开
标题翻译：用拉曼光谱对蛋白质的保护方法PROFILE生物样品
公开(公告)号：EP1711829A2
公开(公告)日：2006-10-18
申请号：EP04815772.1
申请日：2004-12-29
申请人： Intel Corporation
发明人： BERLIN, Andrew , SU, Xing , CHAN, Selena , KOO, Tae-Woong , SUN, Lei , SUNDARARAJAN, Narayan , YAMAKAWA, Mineo
IPC分类号： G01N33/58
CPC分类号： G01N21/658 , G01N33/6803 , G01N2021/653 , G01N2021/655 , G01N2021/656
摘要： The invention provides methods for analyzing the protein content of a biological sample, for example to obtain a protein profile of a sample provided by a particular individual. The proteins and protein fragments in the sample are separated on the basis of chemical and/or physical properties and maintained in a separated state at discrete locations on a solid substrate or within a stream of flowing liquid. Raman spectra are then detected as produced by the separated proteins or fragments at the discrete locations such that a spectrum from a discrete location provides information about the structure or identity of one or more particular proteins or fragments at the discrete location. The proteins or fragments at discrete locations can be coated with a metal, such as gold or silver, and/or the separated proteins can be contacted with a chemical enhancer to provide SERS spectra. Method and kits for practicing the invention are also provided.

52. 发明公开

EP1711624A2 METHODS FOR DETERMINING NUCLEOTIDE SEQUENCE INFORMATION 审中-公开
标题翻译：用于确定的核苷酸序列
公开(公告)号：EP1711624A2
公开(公告)日：2006-10-18
申请号：EP04815742.4
申请日：2004-12-24
申请人： INTEL CORPORATION
发明人： SU, Xing
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q1/6874 , C12Q2565/632
摘要： Provided herein, is a nucleic acid sequencing method based on detection of Raman signatures of oligonucleotide probes. Raman signatures of individually captured nucleic acid probes, optionally labeled by a Raman label or a positively charged enhancer, are detected. The sequences of captured probes are used to identify the nucleotide sequences of captured probes and complementary target nucleic acids, which are then aligned and used to obtain nucleic acid sequence information. In another embodiment, a method is provided for determining a nucleotide occurrence at a target nucleotide position of a target nucleic acid, that utilizes binding of the target nucleic acid to a labeled oligonucleotide probe that binds to the target nucleic acid, wherein the labeled oligonucleotide probe includes a first label and a second label, the first label being capable of affecting an optical property of the second label.

53. 发明公开

EP1675964A1 METHODS AND DEVICE FOR DNA SEQUENCING USING SURFACE ENHANCED RAMAN SCATTERING (SERS) 审中-公开
标题翻译：方法和装置用于DNA测序用SERS（表面增强拉曼散射）
公开(公告)号：EP1675964A1
公开(公告)日：2006-07-05
申请号：EP04788969.6
申请日：2004-09-24
申请人： INTEL CORPORATION
发明人： SU, Xing , CHAN, Selena
IPC分类号： C12Q1/68 , G01N21/65
CPC分类号： C12Q1/6869 , B01L3/5027 , G01N21/65 , G01N21/658 , G01N2021/653 , G01N2021/656 , C12Q2565/632 , C12Q2521/319 , C12Q2563/155 , C12Q2565/629
摘要： The methods and apparatus disclosed herein concern nucleic acid characterization by enhanced Raman spectroscopy. In certain embodiments of the invention, exonuclease treatment of the nucleic acids results in the release of nucleotides. The nucleotides may pass from a reaction chamber through a microfluidic channel and enter a nanochannel or microchannel. The nanochannel or microchannel may be packed with nanoparticle aggregates containing hot spots for Raman detection. As the nucleotides pass through the nanoparticle hot spots, they may be detected by Raman spectroscopy. Identification of the sequence of nucleotides released from the nucleic acid is used to characterize the nucleic acid, for example by sequencing or identifying the nucleic acid. Other embodiments of the invention concern apparatus for nucleic acid sequencing.

54. 发明公开

EP1668164A2 PROGRAMMABLE MOLECULAR BARCODES 审中-公开
标题翻译：可编程分子条形码
公开(公告)号：EP1668164A2
公开(公告)日：2006-06-14
申请号：EP04816244.0
申请日：2004-09-23
申请人： INTEL CORPORATION
发明人： SU, Xing , KOO, Tae-Woong , BERLIN, Andrew , SUN, Lei , SUNDARARAJAN, Narayanan , YAMAKAWA, Mineo
IPC分类号： C12Q1/68
CPC分类号： B82Y10/00 , B82Y5/00 , C12Q1/6816 , G06K19/06028 , C12Q2525/161 , C12Q2537/143 , C12Q2565/1025
摘要： The present disclosure concerns methods for producing and/or using molecular barcodes. In certain embodiments of the invention, the barcodes comprise polymer backbones that may contain one or more branch structures. Tags may be attached to the backbone and/or branch structures. The barcode may also comprise a probe that can bind to a target, such as proteins, nucleic acids and other biomolecules or aggregates. Different barcodes may be distinguished by the type and location of the tags. In other embodiments, barcodes may be produced by hybridization of one or more tagged oligonucleotides to a template, comprising a container section and a probe section. The tagged oligonucleotides may be designed as modular code sections, to form different barcodes specific for different targets. In alternative embodiments, barcodes may be prepared by polymerization of monomeric units. Bound barcodes may be detected by various imaging modalities, such as, surface plasmon resonance, fluorescent or Raman spectroscopy.

55. 发明公开

EP1606610A2 CHEMICAL ENHANCEMENT IN SURFACE ENHANCED RAMAN SCATTERING USING LITHIUM CHLORIDE 审中-公开
标题翻译： FOR增强拉曼散射表面化学BOOST使用氯化锂
公开(公告)号：EP1606610A2
公开(公告)日：2005-12-21
申请号：EP04749311.9
申请日：2004-02-04
申请人： INTEL CORPORATION
发明人： SU, Xing , SUN, Lei , KOO, Tae-Woong , CHAN, Selana
IPC分类号： G01N21/65
CPC分类号： G01J3/44 , B82Y15/00 , C12Q1/6825 , G01N21/658
摘要： The intensity of the signals from surface enhanced Raman spectroscopy is increased by using lithium chloride (122) as an enhancer to activate a metallic structure (212) used for surface enhanced Raman spectroscopy (116). The increased signal intensity allows surface enhanced Raman spectroscopy to be utilized to detect individual analytes (120) such as nucleotides, for example in DNA sequencing without requiring a dye or radioactive label.

56. 发明公开

EP1488002A2 METHODS TO INCREASE NUCLEOTIDE SIGNALS BY RAMAN SCATTERING 审中-公开
标题翻译：方法来增加拉曼NUKLEOTIDSIGNALEN溢油手段
公开(公告)号：EP1488002A2
公开(公告)日：2004-12-22
申请号：EP03719382.8
申请日：2003-03-11
申请人： INTEL CORPORATION
发明人： SU, Xing , BERLIN, Andrew , KOO, Tae-Woong , CHAN, Selena , SUNDARARAJAN, Narayan , YAMAKAWA, Mineo
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , G01N21/29
CPC分类号： C12Q1/6869 , C12Q1/6816 , C12Q1/6825 , C12Q1/6872 , C12Q2565/632 , C12Q2563/155 , C12Q2521/319
摘要： The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation into a nucleic acid (13). Exonuclease (15) treatment of the labeled nucleic acid (13) results in the release of labeled nucleotides (16, 130), which are detected by Raman spectroscopy. In alternative embodiments of the invention, nucleotides (16, 130) released from a nucleic acid (13) by exonuclease (15) treatment are covalently cross-linked to silver or gold nanoparticles (140) and detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERRS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Other embodiments of the invention concern apparatus (10, 100, 210) for nucleic acid sequencing.

57. 发明授权

EP1543152B1 CONTROLLED ALIGNMENT OF NANOBARCODES ENCODING SPECIFIC INFORMATION FOR SCANNING PROBE MICROSCOPY (SPM) READING 有权
标题翻译：纳米条形码的排列受控对探针显微分析具体所包含的信息GRID
公开(公告)号：EP1543152B1
公开(公告)日：2011-03-09
申请号：EP03752088.9
申请日：2003-09-05
申请人： Intel Corporation
发明人： CHAN, Selena , SU, Xing , YAMAKAWA, Mineo
IPC分类号： C12Q1/68 , G01N33/543
CPC分类号： C12Q1/6816 , B82Y5/00 , B82Y10/00 , B82Y30/00 , Y10S977/702 , Y10S977/704 , Y10S977/705 , Y10S977/728 , Y10S977/729 , Y10S977/734 , Y10S977/742 , Y10S977/773 , Y10S977/774 , Y10S977/924 , C12Q2565/601 , C12Q2563/185
摘要： The methods, apparatus and compositions disclosed herein concern the detection, identification and/or sequencing of biomolecules, such as nucleic acids or proteins. In certain embodiments of the invention, coded probes comprising a probe molecule attached to one or more nanobarcodes may be allowed to bind to one or more target molecules. After binding and separation from unbound coded probes, the bound coded probes may be aligned on a surface and analyzed by scanning probe microscopy. The nanobarcodes may be any molecule or complex that is distinguishable by SPM, such as carbon nanotubes, fullerenes, submicrometer metallic barcodes, nanoparticles or quantum dots. Where the probes are oligonucleotides, adjacent coded probes hybridized to a target nucleic acid may be ligated together before alignment and SPM analysis. Compositions comprising coded probes are also disclosed herein. Systems for biomolecule analysis may comprise an SPM instrument and at least one coded probe attached to a surface.
摘要翻译：所述方法，装置和组合物在光盘游离缺失关注的生物分子如，：核酸或蛋白质等的检测，鉴定和/或测序。在本发明的某些实施方案中，包括连接到一个或多个纳米条形码探针分子编码探针可以结合至一个或多个目标分子。与未结合的编码探针的结合和分离后，将结合的编码探针可以在表面上对齐，并且通过扫描探针显微镜分析。纳米条形码可以是任何分子或复合物确实是由SPM区分：例如碳纳米管，富勒烯，亚微米金属条形码，纳米颗粒或量子点。其中探针是寡核苷酸，相邻的编码探针杂交到靶核酸之前对准和扫描探针显微镜（SPM）分析可以连接在一起。组合物，包含编码探针因此光盘游离缺失。用于生物分子分析系统可以包括SPM仪器和附着于表面的至少一个已编码探针的。

58. 发明公开

EP1723232A1 ISOLATING, POSITIONING, AND SEQUENCING SINGLE MOLECULES 审中-公开
标题翻译：绝缘，定位和单个分子的测序
公开(公告)号：EP1723232A1
公开(公告)日：2006-11-22
申请号：EP05713486.8
申请日：2005-02-14
申请人： INTEL CORPORATION
发明人： SU, Xing , SUNDARARAJAN, Narayanan , KOO, Tae-Woong
IPC分类号： C12N15/10 , B01L3/00 , C12Q1/68
CPC分类号： G01N33/54353 , B01L3/5027 , C12N15/1006 , C12Q1/6869 , C12Q2565/629 , C12Q2521/319 , C12Q2565/518
摘要： Devices and methods for isolating, detecting, and positioning single polymeric molecules without the need for expensive equipment are provided. The disclosed devices and methods allow for a molecule to be quickly and efficiently transported to a specific sub-micron area. Such devices are useful, for instance, for performing analyses in which the sequence of a polymer of interest is determined.

59. 发明授权

EP1543328B1 DETECTING MOLECULAR BINDING BY MONITORING FEEDBACK CONTROLLED CANTILEVER DEFLECTIONS 有权
标题翻译：具有约束力的监测反馈来控制BOOM分心分子的检测
公开(公告)号：EP1543328B1
公开(公告)日：2006-11-22
申请号：EP03770419.4
申请日：2003-09-24
申请人： INTEL CORPORATION
发明人： SU, Xing , CHAN, Selena , KOO, Tae-Woong , YAMAKAWA, Mineo , BERLIN, Andrew
IPC分类号： G01N33/543 , C12Q1/68 , G01B7/34
CPC分类号： C12Q1/6825 , B82Y5/00 , G01N33/54373 , G01N2800/52 , Y10S977/924 , C12Q2565/501
摘要： The present methods and apparatus concern the detection and/or identification of target analytes using probe molecules. In various embodiments of the invention, the probes or analytes are attached to one or more cantilevers. Binding of a probe to an analyte results in deflection of the cantilever, detected by a detection unit. A counterbalancing force may be applied to restore the cantilever to its original position. The counterbalancing force may be magnetic, electrical or radiative. The detection unit and the mechanism generating the counterbalancing force may be operably coupled to an information processing and control unit, such as a computer. The computer may regulate a feedback loop that maintains the cantilever in a fixed position by balancing the deflecting force and the counterbalancing force. The concentration of analytes in a sample may be determined from the magnitude of the counterbalancing force required to maintain the cantilever in a fixed position.

60. 发明公开

EP1699938A2 METHODS OF PRODUCING CARBON NANOTUBES USING PEPTIDE OR NUCLEIC ACID MICROPATTERNING 审中-公开
标题翻译：工艺制造碳纳米管的使用肽或核酸MICRO图案的生产
公开(公告)号：EP1699938A2
公开(公告)日：2006-09-13
申请号：EP04818073.1
申请日：2004-12-24
申请人： INTEL CORPORATION
发明人： SU, Xing , SUNDARARAJAN, Narayan , YAMAKAWA, Mineo , BERLIN, Andrew , SUN, Lei , ZHANG, Yuegang
IPC分类号： C12Q1/68
CPC分类号： B82Y40/00 , B82Y10/00 , B82Y30/00 , C01B32/162 , C01B2202/08 , C01B2202/36 , D01F9/127 , H01L51/0048 , H01L51/0052
摘要： The methods, apparatus and systems disclosed herein concern ordered arrays of carbon nanotubes. In particular embodiments of the invention, the nanotube arrays are formed by a method comprising attaching catalyst nanoparticles (140, 230) to polymer (120, 210) molecules, attaching the polymer (120, 210) molecules to a substrate, removing the polymer (120, 210) molecules and producing carbon nanotubes on the catalyst nanoparticles (140, 230). The polymer (120, 210) molecules alignment techniques. The nanotube arrays can be attached to selected areas (110, 310) of the substrate. Within the selected areas (110, 310), the nanotubes are distributed non-randomly. Other embodiments disclosed herein concern apparatus that include ordered arrays of nanotubes attached to a substrate and systems that include ordered arrays of carbon nanotubes attached to a substrate, produced by the claimed methods. In certain embodiments, provided herein are methods for aligning a molecular wire, by ligating the molecular wire to a double stranded DNA molecule.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式