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51. 发明公开

EP2496603A1 GLYCOSYLATED REPEAT-MOTIF-MOLECULE CONJUGATES 有权
标题翻译： Glycosylierte Repeat-Motiv-Molekülkonjugate
公开(公告)号：EP2496603A1
公开(公告)日：2012-09-12
申请号：EP10779694.8
申请日：2010-11-04
申请人： F. Hoffmann-La Roche AG
发明人： FISCHER, Stephan , IMHOF-JUNG, Sabine , KOPETZKI, Erhard
IPC分类号： C07K16/18 , C07K16/00 , C07K16/32 , C07K16/46 , C07K14/47
CPC分类号： C07K16/18 , C07K16/00 , C07K16/32 , C07K16/468 , C07K2317/41 , C07K2317/52 , C07K2318/20 , C07K2319/00 , C07K2319/21 , C07K2319/30
摘要： Herein are reported glycosylated repeat-motif-molecule conjugate of the following formula: (repeat-motif-molecule−linkern)m−conjugation partner−(linkero−repeat-motif-molecule)p, wherein n and o are independently of each other and independently for each value of m and p integer values of 0 or 1, and m and p are independently of each other integer values of 0 or 1 or 2 or 3 or 4 or 5 or 6 or 7, and wherein the repeat-motif-molecule conjugate comprises at least one oligosaccharide attached to a glycosylation site. Also reported are encoding nucleic acids and method for producing these repeat-motif-conjugates in mammalian cells.
摘要翻译：本文报导了下式的糖基化重复 - 基序 - 分子结合物：（重复 - 基序 - 分子连接）m-缀合配偶体（连接重复 - 基序 - 分子）p，其中n和o彼此独立地为对于m和p整数值为0或1的每个值独立地为m和p彼此独立地为0或1或2或3或4或5或6或7的整数，并且其中重复 - 分子结合物包含至少一个连接于糖基化位点的寡糖。还报道了编码核酸和在哺乳动物细胞中产生这些重复基序缀合物的方法。

52. 发明授权

EP2164972B1 PROMOTER 有权
标题翻译：启动子
公开(公告)号：EP2164972B1
公开(公告)日：2011-11-30
申请号：EP08773642.7
申请日：2008-06-25
申请人： F. Hoffmann-La Roche AG
发明人： KLEIN, Christian , KOPETZKI, Erhard
IPC分类号： C12N15/85 , C12N15/11
CPC分类号： C12N15/63 , C07K14/43595 , C07K14/4702 , C12N15/85
摘要： The current invention reports a promoter having the nucleic acid sequence of SEQ ID NO: 02, or SEQ ID NO: 03, or SEQ ID NO: 04, or SEQ ID NO: 06, which is a 5′ shortened SV40 promoter with reduced promoter strength especially useful for the limited expression of heterologous polypeptides or selectable markers.

53. 发明公开

EP2207882A1 CHO-K1 CELL LINE 审中-公开
标题翻译： CHO-K1-ZELLLINIE
公开(公告)号：EP2207882A1
公开(公告)日：2010-07-21
申请号：EP08836958.2
申请日：2008-10-13
申请人： F. Hoffmann-La Roche AG
发明人： KOPETZKI, Erhard , SCHWARZ, Ursula
IPC分类号： C12N15/69 , C12N15/85 , C07K16/00
CPC分类号： C12N15/85 , C07K16/18 , C07K16/28 , C07K16/2812 , C07K16/2854 , C07K16/2866 , C07K16/2896 , C07K2317/14 , C07K2317/21 , C07K2317/51 , C07K2317/515 , C12P21/00
摘要： The current invention reports a CHO-K1 cell, characterized in that said CHO-K1 cell is derived from CHO-K1 cell deposited as ATCC CCL-61, grows in suspension, requires no glutamine, no insulin, and no growth factors in the cultivation medium for growth, whereby said CHO-K1 cell is not modified compared to the deposited CHO-K1 cell ATCC CCL-61 cell line by the introduction, deletion, or inactivation of a nucleic acid. Also reported are a method for obtaining said CHO-K1 cell and a method for the production of a heterologous polypeptide using such a CHO-K1 cell according to the invention.
摘要翻译：本发明报道了在CHO细胞中重组产生分泌的异源免疫球蛋白的方法，其包括以下步骤：i）提供适于在悬浮培养物中生长的CHO细胞，适于在无血清培养基中生长，支原体免费和无病毒，ii）提供包含原核复制起点的载体，赋予原核选择剂抗性的第一核酸，编码所述异源免疫球蛋白的重链的第二核酸，编码光的第三核酸所述异源免疫球蛋白的链，赋予对真核选择剂的抗性的第四核酸，iii）转染所述CHO细胞，其中所述转染包括a）用所述载体转染所述CHO细胞，所述载体包含赋予对第一真核选择性的抗性的第四核酸试剂，b）通过在含有所述第一种真核生物的培养基中生长来选择CHO细胞 c）用所述载体转染所述选择的CHO细胞，所述载体包含赋予与所述第一真核选择剂不同的第二真核选择剂的抗性的第四核酸，d）通过在含有所述第一和第二真核选择剂的培养基中选择生长来选择CHO细胞，所述第二真核选择剂，iv）在所述第一和第二真核选择剂的存在下，在适于表达所述第二和第三核酸的条件下，在介质中培养所述转染的CHO细胞，和v）回收所述分泌的异源免疫球蛋白。

54. 发明公开

EP2167650A1 CHO CELL 审中-公开
标题翻译： CHO细胞
公开(公告)号：EP2167650A1
公开(公告)日：2010-03-31
申请号：EP08773641.9
申请日：2008-06-25
申请人： F. Hoffmann-La Roche AG
发明人： DE LANGE, Robert , KOPETZKI, Erhard
IPC分类号： C12N5/10 , C12P21/02
CPC分类号： C07K14/72 , C12N2501/105 , C12N2501/998 , C12N2510/02
摘要： The current invention comprises a CHO cell which is expressing a constitutively active mitogenic receptor, a method of obtaining such a CHO cell, and a method for the expression of a heterologous polypeptide using a CHO cell according to the invention.

55. 发明公开

EP2167540A1 HEAVY CHAIN MUTANT LEADING TO IMPROVED IMMUNOGLOBULIN PRODUCTION 有权转让
标题翻译：重链突变体改进IMMUNOGLOBULINHERSTELLUNG
公开(公告)号：EP2167540A1
公开(公告)日：2010-03-31
申请号：EP08773643.5
申请日：2008-06-25
申请人： F. Hoffmann-La Roche AG
发明人： GOEPFERT, Ulrich , HANSEN, Silke , KNOETGEN, Hendrik , KOPETZKI, Erhard , PLOETTNER, Oliver
IPC分类号： C07K16/00 , C12N15/09 , C12N15/67 , C12N15/00
CPC分类号： C07K16/00 , C07K2317/21 , C07K2317/52 , C12N2830/42
摘要： The current invention comprises a nucleic acid encoding the amino acid sequence of the C-terminal part of the CH3-domain of an immunoglobulin of the class IgA or IgG, or the C-terminal part of the CH4-domain of an immunoglobulin of the class IgE or IgM, wherein the glycine-lysine-dipeptide comprised in the amino acid sequence of the C-terminal part of the CH3- or CH4-domain is encoded by the nucleic acid ggaaaa, or the nucleic acid ggcaaa, or the nucleic acid gggaaa, or the nucleic acid gggaag, or the nucleic acid ggcaag, or the nucleic acid ggaaag.

56. 发明公开

EP3464345A1 FUSION PROTEINS FOR OPHTHALMOLOGY WITH INCREASED EYE RETENTION 审中-公开
公开(公告)号：EP3464345A1
公开(公告)日：2019-04-10
申请号：EP17728816.4
申请日：2017-06-02
申请人： F. Hoffmann-La Roche AG
发明人： HUELSMANN, Peter Michael , KOPETZKI, Erhard
IPC分类号： C07K14/78 , A61K39/00 , C07K16/18

57. 发明公开

EP3138917A1 METHOD FOR THE EXPRESSION OF POLYPEPTIDES USING MODIFIED NUCLEIC ACIDS 有权
标题翻译： VERFAHREN ZUR EXPRESSION VON POLYPEPTIDEN MIT MODIFIZIERTENNUKLEINSÄUREN
公开(公告)号：EP3138917A1
公开(公告)日：2017-03-08
申请号：EP16188552.0
申请日：2013-04-15
申请人： F. Hoffmann-La Roche AG
发明人： KLOSTERMANN, Stefan , KOPETZKI, Erhard , SCHWARZ, Ursula
IPC分类号： C12N15/67
CPC分类号： C07K16/00 , C07K2317/14 , C12N15/67
摘要： Herein is reported a method for recombinantly producing a polypeptide in a cell comprising the step of cultivating a cell which comprises a nucleic acid encoding the polypeptide, and recovering the polypeptide from the cell or the cultivation medium, wherein each of the amino acid residues of the polypeptide is encoded by at least one codon, whereby the different codons encoding the same amino acid residue are combined in one group and each of the codons in a group is defined by a specific usage frequency within the group, whereby the sum of the specific usage frequencies of all codons in one group is 100 %, and wherein the usage frequency of a codon in the polypeptide encoding nucleic acid is about the same as its specific usage frequency within its group.
摘要翻译：本文报道了一种在细胞中重组产生多肽的方法，包括培养包含编码该多肽的核酸的细胞的步骤，以及从该细胞或培养基中回收多肽，其中每个氨基酸残基多肽由至少一个密码子编码，由此将编码相同氨基酸残基的不同密码子组合在一组中，组中的每个密码子由组内的特定使用频率定义，由此具体用途的总和一组中所有密码子的频率为100％，其中编码多肽的核酸中密码子的使用频率与其组内的特定使用频率大致相同。

58. 发明授权

EP1613658B1 ANTIBODIES AGAINST INSULIN-LIKE GROWTH FACTOR I RECEPTOR AND USES THEREOF 有权
标题翻译：抗体的胰岛素样生长因子受体及其用途
公开(公告)号：EP1613658B1
公开(公告)日：2012-03-14
申请号：EP04725024.6
申请日：2004-04-01
申请人： F. Hoffmann-La Roche AG
发明人： GRAUS, Yvo , KOPETZKI, Erhard , KUENKELE, Klaus-Peter , MUNDIGL, Olaf , PARREN, Paul , REBERS, Frank , SCHUMACHER, Ralf , VAN DE WINKEL, Jan , VAN VUGT, Martine
IPC分类号： C07K16/28 , A61K39/395 , C12N5/20 , C12N15/13 , C12N15/85 , C12N5/10 , A61P35/00
CPC分类号： C07K16/2863 , A61K2039/505 , C07K2317/21 , C07K2317/52 , C07K2317/56 , C07K2317/565
摘要： An antibody binding to IGF-IR and inhibiting the binding of IGF-I and IGF-II to IG-RI which is characterized in that said antibody is of human IgG1 isotype, and shows a ratio of inhibition of the binding of IGF-I to IGF-IR to the inhibition of binding of IGF-II to IGF-IR of 1:3 to 3:1, and induces celle death of 20% or more cells of a preparation of IGF-IR expressing cells after 24 hours at a concentration of said antibody of 100 nM by ADCC; has improved properties in antitumor therapy.

59. 发明公开

EP2027273A1 POLYPEPTIDE PRODUCING CELLS 有权
标题翻译：多肽生产细胞
公开(公告)号：EP2027273A1
公开(公告)日：2009-02-25
申请号：EP07725230.2
申请日：2007-05-15
申请人： F. Hoffmann-La Roche AG
发明人： ENDL, Josef , KOPETZKI, Erhard , PLOETTNER, Oliver , SCHWARZ, Ursula , TIEFENTHALER, Georg
IPC分类号： C12N15/82
CPC分类号： C12N15/85 , C07K16/00 , C07K16/065 , C07K2319/03
摘要： The current invention describes a nucleic acid comprising in a 5' to 3' direction a) a first nucleic acid encoding a heterologous polypeptide without an in frame stop codon, b) a second nucleic acid beginning with a 5' splice donor site and terminated by a 3' splice acceptor site comprising an in frame translational stop codon and a polyadenylation signal, and c) a nucleic acid encoding i) at least a fragment of a transmembrane domain, or ii) a signal peptide for a GPI-anchor.
摘要翻译：本发明描述了一种核酸，其在5'至3'方向上包含a）编码不具有框内终止密码子的异源多肽的第一核酸，b）以5'剪接供体位点开始并终止于第二核酸包含框内翻译终止密码子和聚腺苷酸化信号的3'剪接受体位点，和c）编码i）跨膜结构域的至少一个片段的核酸，或ii）用于GPI-锚的信号肽。

60. 发明授权

EP3138917B1 METHOD FOR THE EXPRESSION OF POLYPEPTIDES USING MODIFIED NUCLEIC ACIDS 有权
公开(公告)号：EP3138917B1
公开(公告)日：2019-08-21
申请号：EP16188552.0
申请日：2013-04-15
申请人： F. Hoffmann-La Roche AG
发明人： KLOSTERMANN, Stefan , KOPETZKI, Erhard , SCHWARZ, Ursula
IPC分类号： C12N15/67

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