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51. 发明授权

US5332845A Phosphorylating reagents 失效
标题翻译：磷酸化试剂
公开(公告)号：US5332845A
公开(公告)日：1994-07-26
申请号：US23932
申请日：1993-02-26
申请人： Michael S. Ureda , Thomas Horn
发明人： Michael S. Ureda , Thomas Horn
IPC分类号： C07F9/09 , C07F9/24 , C07H19/16 , C07H21/00 , C12P19/34 , C07F9/141
CPC分类号： C07H21/00 , C07F9/091 , C07F9/2408 , C07H19/16 , C12P19/34
摘要： Novel phosphorylating reagents are provided which are useful in DNA synthesis and purification procedures. The reagents are substituted phosphines which are particularly useful in the phosphorylation of nucleoside and solid supported oligonucleotides at the 5'-hydroxyl position. Phosphorylation using these reagents is easily and accurately monitored colorimetrically.
摘要翻译：提供了可用于DNA合成和纯化方法的新型磷酸化试剂。试剂是在5'-羟基位置的核苷和固体负载的寡核苷酸的磷酸化中特别有用的取代的膦。使用这些试剂进行磷酸化可以用比色容易且准确的监测。

52. 发明授权

US5256549A Purification of synthetic oligomers 失效
标题翻译：合成低聚物的纯化
公开(公告)号：US5256549A
公开(公告)日：1993-10-26
申请号：US517526
申请日：1990-04-27
申请人： Michael S. Urdea , Thomas Horn
发明人： Michael S. Urdea , Thomas Horn
IPC分类号： C07H21/00 , C12P19/34 , C07K1/04 , C07K1/06 , C07K1/14
CPC分类号： C07H21/00 , C12P19/34 , Y02P20/55
摘要： Oligomers and polymers are prepared substantially free of error sequences by sequentially adding monomers, which are terminally blocked and have active functionalities protected, to a growing chain bound to a support through a selectively cleavable linkage. After each addition, unblocked terminal groups are capped. At the completion of monomer addition, enzymatic hydrolysis interfering protecting groups are removed along with the capping group and failure sequences enzymatically degraded. The terminal blocking group may then be removed. The completed oligomer or polymer may be cleaved from the support prior or subsequent to enzymatic degradation but after completion of the sequence.
摘要翻译：低聚物和聚合物基本上不含错误序列，通过将通过末端封闭并具有活性官能团保护的单体通过可选择性切割的键连接到与载体结合的生长链中而制备。每次添加后，未封锁的终端组被封顶。在单体添加完成时，酶水解干扰保护基与封端基一起去除并且酶降解的失效序列。然后可以去除端子阻挡组。完成的低聚物或聚合物可以在酶降解之前或之后但在序列完成后从载体上切割。

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