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51. 发明授权

US06632673B1 Directing the ratio of B2:B1 avermectins in Streptomyces avermitilis host cells 有权
标题翻译：指导阿维链霉菌宿主细胞中B2：B1阿凡曼菌素的比例
公开(公告)号：US06632673B1
公开(公告)日：2003-10-14
申请号：US09637040
申请日：2000-08-10
申请人： Kim J. Stutzman-Engwall , Yan Chen , Claes Gustafsson , Anke Krebber , Jeremy Minshull , Sun Ai Raillard
发明人： Kim J. Stutzman-Engwall , Yan Chen , Claes Gustafsson , Anke Krebber , Jeremy Minshull , Sun Ai Raillard
IPC分类号： C12N1574
CPC分类号： C12P17/181 , C12N9/88 , C12P19/623
摘要： The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
摘要翻译：本发明涉及包含编码aveC基因产物的核苷酸序列的多核苷酸分子，所述多核苷酸分子可用于改变在发酵培养物中产生的2类1阿维菌素的比例或量。阿维菌素。本发明还涉及载体，宿主细胞和 S的突变株。阿维菌素，其中aveC基因已被灭活或突变，以便改变产生的2：1类阿维菌素的比例或量。

52. 发明授权

US08401798B2 Systems and methods for constructing frequency lookup tables for expression systems 有权
标题翻译：用于构建表达系统的频率查找表的系统和方法
公开(公告)号：US08401798B2
公开(公告)日：2013-03-19
申请号：US12184233
申请日：2008-07-31
申请人： Mark Welch , Claes Gustafsson
发明人： Mark Welch , Claes Gustafsson
IPC分类号： G01N33/34 , G06F19/00
CPC分类号： C12Q1/68 , G06F19/20 , G06F19/22 , G06F19/24
摘要： Methods for determining a property that affects expression of polynucleotides are provided. A plurality of polynucleotides each encoding a polypeptide sequence is constructed. A frequency that a sequence element is used in a first polynucleotide is different than in a second polynucleotide. Each polynucleotide is expressed in an expression system to obtain an expression property value thereby constructing a dataset that contains, for each respective polynucleotide, sequence element occurrence in the respective polynucleotide and the measured expression property value of the respective polynucleotide. A model is computed that describes variation in the measured expression property values as a function of a plurality of variables and weights. From the model, a property that affects expression of polynucleotides in the expression system is determined, where the property is an effect that the frequency of occurrence of one or more sequence elements has on the expression property of polynucleotides in the expression system.
摘要翻译：提供了确定影响多核苷酸表达的性质的方法。构建多个编码多肽序列的多核苷酸。在第一多核苷酸中使用序列元件的频率与第二多核苷酸不同。每个多核苷酸在表达系统中表达以获得表达属性值，由此构建对于各个多核苷酸包含各个多核苷酸中的序列元件出现和各个多核苷酸的测量的表达特性值的数据集。计算描述作为多个变量和权重的函数的测量表达式属性值的变化的模型。从该模型中，确定影响表达系统中多核苷酸表达的性质，其中性质是一种或多种序列元件的出现频率对表达系统中多核苷酸的表达性质的影响。

53. 发明授权

US07957912B2 Methods for identifying and producing polypeptides 有权
标题翻译：用于鉴定和产生多肽的方法
公开(公告)号：US07957912B2
公开(公告)日：2011-06-07
申请号：US12557463
申请日：2009-09-10
申请人： Sergey A. Selifonov , Willem P. C. Stemmer , Claes Gustafsson , Matthew Tobin , Stephen del Cardayre , Phillip A. Patten , Jeremy Minshull , Lorraine J. Giver
发明人： Sergey A. Selifonov , Willem P. C. Stemmer , Claes Gustafsson , Matthew Tobin , Stephen del Cardayre , Phillip A. Patten , Jeremy Minshull , Lorraine J. Giver
IPC分类号： G01N33/48 , C12Q1/00 , C12Q1/48 , C07H21/02 , C07H21/04
CPC分类号： G06F19/16 , B01J2219/007 , C40B50/02 , G06F19/14 , G06F19/18 , G06F19/22 , G06F19/24
摘要： In silico nucleic acid recombination methods, related integrated systems utilizing genetic operators and libraries made by in silico shuffling methods are provided. One disclosed method involves identifying and producing a polypeptide. The method may involve (a) receiving data identifying sequences of two or more parental polypeptides or parental nucleic acids that encode the polypeptides; (b) selecting one or more cross-over sites on the sequences thereby defining one or more recombinant polypeptides or recombinant nucleic acids; (c) selecting at least one of the recombinant polypeptides or recombinant nucleic acids; (d) recombining one or more oligonucleotides, at least one of which has a sequence matching some or all of that of the recombinant polypeptides or recombinant nucleic acids selected in (c); (e) selecting at least some of the recombined oligonucleotides produced in (d); and (f) producing a polypeptide encoded by the selected nucleic acid.
摘要翻译：在硅胶核酸重组方法中，提供了利用遗传算子和通过计算机改组方法制备的文库的相关综合系统。一种公开的方法涉及鉴定和产生多肽。该方法可以包括（a）接收识别编码多肽的两个或多个亲本多肽或亲本核酸的序列的数据; （b）在序列上选择一个或多个交叉位点，从而限定一个或多个重组多肽或重组核酸; （c）选择至少一种重组多肽或重组核酸; （d）重组一种或多种寡核苷酸，其中至少一种具有与（c）中选择的重组多肽或重组核酸的部分或全部相匹配的序列; （e）选择（d）中产生的至少一些重组寡核苷酸; 和（f）产生由所选择的核酸编码的多肽。

54. 发明申请

US20100261660A1 Novel co-stimulatory molecules 审中-公开
标题翻译：新型共刺激分子
公开(公告)号：US20100261660A1
公开(公告)日：2010-10-14
申请号：US11645361
申请日：2006-12-22
申请人： Juha Punnonen , Alexandra Lazetic , Steven R. Leong , Chia-Chun Chang , Doris Apt , Claes Gustafsson
发明人： Juha Punnonen , Alexandra Lazetic , Steven R. Leong , Chia-Chun Chang , Doris Apt , Claes Gustafsson
IPC分类号： A61K38/16 , C07K14/00 , C07K19/00 , C12N15/11 , C12N5/10 , C12N1/19 , C12N1/21 , C12N15/63 , C12P21/00 , A61P37/00
CPC分类号： C07K14/70503 , A61K38/00 , C07K14/70532
摘要： The invention provides polynucleotides and polypeptides encoded therefrom having advantageous properties, including an ability of the polypeptides to preferentially bind a CD28 or CTLA-4 receptor at a level greater or less than the ability of human B7-1 to bind CD28 or CTLA-4, or to induce or inhibit altered level of T cell proliferation response greater compared to that generated by human B7-1. The polypeptides and polynucleotides of the invention are useful in therapeutic and prophylactic treatment methods, gene therapy applications, and vaccines.
摘要翻译：本发明提供了由其编码的多核苷酸和多肽，其具有有利的性质，包括多肽以大于或小于人B7-1结合CD28或CTLA-4的能力优先结合CD28或CTLA-4受体的能力，或诱导或抑制T细胞增殖反应的改变水平与人B7-1产生的相比更大。本发明的多肽和多核苷酸可用于治疗和预防性治疗方法，基因治疗应用和疫苗。

55. 发明申请

US20100184627A1 OLIGONUCLEOTIDE MEDIATED NUCLEIC ACID RECOMBINATION 有权
标题翻译：寡核苷酸介导的核酸重组
公开(公告)号：US20100184627A1
公开(公告)日：2010-07-22
申请号：US12557829
申请日：2009-09-11
申请人： Andreas Crameri , Willem P.C. Stemmer , Jeremy Minshull , Steven H. Bass , Mark Welch , John E. Ness , Claes Gustafsson , Phillip A. Patten
发明人： Andreas Crameri , Willem P.C. Stemmer , Jeremy Minshull , Steven H. Bass , Mark Welch , John E. Ness , Claes Gustafsson , Phillip A. Patten
IPC分类号： C40B50/00
CPC分类号： C12P21/06
摘要： Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.
摘要翻译：提供重组核酸的方法，包括同源核酸。还提供了基因改组寡核苷酸家族及其在重组过程中的应用，以及聚合酶和连接酶介导的重组方法。

56. 发明申请

US20100056385A1 METHODS FOR IDENTIFYING SETS OF OLIGONUCLEOTIDES FOR USE IN AN IN VITRO RECOMBINATION PROCEDURE 有权
标题翻译：用于鉴定体外重组程序中使用寡核苷酸的方法
公开(公告)号：US20100056385A1
公开(公告)日：2010-03-04
申请号：US12557463
申请日：2009-09-10
申请人： Sergey A. Selifonov , Willem P.C. Stemmer , Claes Gustafsson , Matthew Tobin , Stephen del Cardayre , Phillip A. Patten , Jeremy Minshull , Lorraine J. Giver
发明人： Sergey A. Selifonov , Willem P.C. Stemmer , Claes Gustafsson , Matthew Tobin , Stephen del Cardayre , Phillip A. Patten , Jeremy Minshull , Lorraine J. Giver
IPC分类号： C40B30/02
CPC分类号： G06F19/16 , B01J2219/007 , C40B50/02 , G06F19/14 , G06F19/18 , G06F19/22 , G06F19/24
摘要： In silico nucleic acid recombination methods, related integrated systems utilizing genetic operators and libraries made by in silico shuffling methods are provided.
摘要翻译：在硅胶核酸重组方法中，提供了利用遗传算子和通过计算机改组方法制备的文库的相关综合系统。

57. 发明申请

US20080050782A1 Methods for making character strings, polynucleotides and polypeptides having desired characteristics 有权
公开(公告)号：US20080050782A1
公开(公告)日：2008-02-28
申请号：US11975638
申请日：2007-10-18
申请人： Sergey Selifonov , Willem Stemmer , Claes Gustafsson , Matthew Tobin , Stephen Cardayre , Phillip Patten , Jeremy Minshull , Lorraine Giver
发明人： Sergey Selifonov , Willem Stemmer , Claes Gustafsson , Matthew Tobin , Stephen Cardayre , Phillip Patten , Jeremy Minshull , Lorraine Giver
IPC分类号： C12P19/34 , C40B30/02 , C40B60/04
CPC分类号： G06F19/16 , B01J2219/007 , C40B50/02 , G06F19/14 , G06F19/18 , G06F19/22 , G06F19/24
摘要： In silico nucleic acid recombination methods, related integrated systems utilizing genetic operators and libraries made by in silico shuffling methods are provided.

58. 发明申请

US20070065838A1 OLIGONUCLEOTIDE MEDIATED NUCLEIC ACID RECOMBINATION 审中-公开
标题翻译：寡核苷酸介导的核酸重组
公开(公告)号：US20070065838A1
公开(公告)日：2007-03-22
申请号：US11277337
申请日：2006-03-23
申请人： Andreas Crameri , Willem Stemmer , Jeremy Minshull , Steven Bass , Mark Welch , John Ness , Claes Gustafsson , Phillip Patten
发明人： Andreas Crameri , Willem Stemmer , Jeremy Minshull , Steven Bass , Mark Welch , John Ness , Claes Gustafsson , Phillip Patten
IPC分类号： C40B30/06 , C40B40/02 , C40B40/08 , C12Q1/68 , C12P19/34 , C07H21/04 , C12N15/86
CPC分类号： C12P21/06
摘要： Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.
摘要翻译：提供重组核酸的方法，包括同源核酸。还提供了基因改组寡核苷酸家族及其在重组过程中的应用，以及聚合酶和连接酶介导的重组方法。

59. 发明申请

US20060051795A1 Oligonucleotide mediated nucleic acid recombination 审中-公开
公开(公告)号：US20060051795A1
公开(公告)日：2006-03-09
申请号：US11203602
申请日：2005-08-15
申请人： Andreas Crameri , Willem Stemmer , Jeremy Minshull , Steven Bass , Mark Welch , Jon Ness , Claes Gustafsson , Phillip Patten
发明人： Andreas Crameri , Willem Stemmer , Jeremy Minshull , Steven Bass , Mark Welch , Jon Ness , Claes Gustafsson , Phillip Patten
IPC分类号： C12Q1/68
CPC分类号： C12N15/1031 , B01J2219/00689
摘要： Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.

60. 发明授权

US06436675B1 Use of codon-varied oligonucleotide synthesis for synthetic shuffling 失效
标题翻译：使用密码子变化的寡核苷酸合成进行合成洗牌
公开(公告)号：US06436675B1
公开(公告)日：2002-08-20
申请号：US09408393
申请日：1999-09-28
申请人： Mark Welch , Jon Ness , Claes Gustafsson , Willem P. C. Stemmer , Jeremy Minshull
发明人： Mark Welch , Jon Ness , Claes Gustafsson , Willem P. C. Stemmer , Jeremy Minshull
IPC分类号： C12Q168
CPC分类号： C12N15/1031 , B01J2219/00689 , C12N15/11
摘要： Methods of providing shuffling libraries that include codon-varied oligonucleotide sequences are described. Codon-varied oligonucleotides are synthesized using trinucleotide or mononucleotide phosphoramidite sequences, and are derived from homologous or non-homologous nucleic acid sequences, or combinations of such sequences. Various methods of recombining codon-varied oligonucleotide sequences to expedite artificial evolution are also described. The present invention additionally relates to various integrated systems that are optionally used to automate these recombination methods.
摘要翻译：描述了提供包括密码子变化的寡核苷酸序列的改组文库的方法。使用三核苷酸或单核苷酸亚磷酰胺序列合成密码子多样的寡核苷酸，并衍生自同源或非同源核酸序列或这些序列的组合。还描述了重组密码子变化的寡核苷酸序列以加速人造进化的各种方法。本发明还涉及可选地用于使这些重组方法自动化的各种集成系统。

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