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51. 发明申请

US20080014608A1 Modified Promoter 失效
标题翻译：修饰的启动子
公开(公告)号：US20080014608A1
公开(公告)日：2008-01-17
申请号：US10589960
申请日：2005-03-04
申请人： Keiji Endo , Katsuya Ozaki
发明人： Keiji Endo , Katsuya Ozaki
IPC分类号： C12P21/04 , C07H21/04 , C12N1/20 , C12N15/00 , C12N15/11
CPC分类号： C12N15/75 , C07K14/32 , C12N9/2408 , C12N9/2411 , C12N9/2417 , C12N9/2437
摘要： The present invention provides a modified promoter DNA capable of enhancing transcription of genes encoding proteins or polypeptides, and a method for producing proteins or polypeptides efficiently by use of the modified promoter DNA.A promoter DNA recognized by SigA and SigE, which is produced by modifying a nucleotide sequence including a promoter recognized by SigA and bases in the vicinity thereof; an expression vector harboring the promoter DNA; a recombinant microorganism containing the expression vector; and a method for producing proteins or polypeptides characterized by culturing the recombinant microorganism.
摘要翻译：本发明提供能够增强编码蛋白质或多肽的基因的转录的修饰的启动子DNA，以及通过使用经修饰的启动子DNA有效产生蛋白质或多肽的方法。由SigA和SigE鉴定的启动子DNA，其通过修饰包括SigA识别的启动子和其附近的碱基的核苷酸序列而产生; 携带启动子DNA的表达载体; 含有表达载体的重组微生物; 以及产生以培养重组微生物为特征的蛋白质或多肽的方法。

52. 发明申请

US20050181493A1 Mutant alpha-amylases 审中-公开
标题翻译：突变型α-淀粉酶
公开(公告)号：US20050181493A1
公开(公告)日：2005-08-18
申请号：US10988529
申请日：2004-11-16
申请人： Keiji Endo , Kazuaki Igarashi , Yasuhiro Hayashi , Hiroshi Hagihara , Katsuya Ozaki
发明人： Keiji Endo , Kazuaki Igarashi , Yasuhiro Hayashi , Hiroshi Hagihara , Katsuya Ozaki
IPC分类号： C11D3/386 , C12N1/21 , C12N9/28 , C12N15/56 , C11D7/42
CPC分类号： C12N9/2417 , C11D3/386
摘要： The invention relates to a mutant α-amylase obtained by making replacement or deletion of at least one of amino acid residues such as the 167th Gln, 169th Tyr and 178th Ala in the amino acid sequence set forth in SEQ ID NO:1 in an α-amylase having said amino acid sequence, or an α-amylase having a homology of at least 70% to said amino acid sequence, a gene encoding the mutant α-amylase, a vector, transformed cells, a process for producing a mutant α-amylase, comprising culturing the transformed cells, and a detergent composition comprising the mutant α-amylase. The mutant α-amylase of the invention has excellent properties of high resistance to chelating agents, high specific activity in an alkaline region and excellent stability to heat, and is hence useful for detergents for automatic dish washer, laundry detergents and the like.
摘要翻译：本发明涉及通过使SEQ ID NO：1所示的氨基酸序列中的至少一个氨基酸残基例如第167位Gln，第169位和第178位氨基酸置换成α位点而获得的突变型α-淀粉酶具有所述氨基酸序列的淀粉酶或与所述氨基酸序列具有至少70％同源性的α-淀粉酶，编码突变型α-淀粉酶的基因，载体，转化细胞，用于产生突变型α-淀粉酶的方法，包括培养转化细胞的淀粉酶和包含突变型α-淀粉酶的洗涤剂组合物。本发明的突变型α-淀粉酶具有优异的抗螯合剂抗性，在碱性区域中具有高比活性和优异的耐热稳定性，因此可用于自动洗碗机，衣物洗涤剂等的洗涤剂。

53. 发明授权

US4654074A 2-chloro-N-(4,6-dimethoxy-5-pyrimidinyl)-N-[1,(1-py razolyl)ethyl]acetamide, herbicidal composition comprising said compound, and method of controlling weeds using said compound 失效
标题翻译： 2-氯-N-（4,6-二甲氧基-5-嘧啶基）-N- [1,1-（吡唑基）乙基]乙酰胺，包含所述化合物的除草组合物和使用所述化合物控制杂草的方法
公开(公告)号：US4654074A
公开(公告)日：1987-03-31
申请号：US829995
申请日：1986-02-18
申请人： Atsushi Goh , Mami Nakamura , Keiji Endo , Mitsuru Hikido
发明人： Atsushi Goh , Mami Nakamura , Keiji Endo , Mitsuru Hikido
IPC分类号： C07D403/12 , A01N43/56 , C07D521/00 , C07D247/02
CPC分类号： C07D231/12 , A01N43/56 , C07D233/56 , C07D249/08
摘要： 2-Chloro-N-(4,6-dimethoxy-5-pyrimidinyl)-N-[1-(1-pyrazolyl)ethyl]acetamide of the following formula: ##STR1## a herbicidal composition comprising a herbicidally effective amount of the compound of formula (I); and a method of controlling weeds which comprises applying a herbicidally effective amount of the formula (I) to the locus where the weeds are growing or will grow.
摘要翻译：具有下式的2-氯-N-（4,6-二甲氧基-5-嘧啶基）-N- [1-（1-吡唑基）乙基]乙酰胺：（I）除草组合物，其包含除草有效式（I）化合物的量; 以及控制杂草的方法，其包括将除草有效量的式（I）施用于杂草生长或将要生长的场所。

54. 发明授权

US4378990A Herbicidal composition 失效
标题翻译：除草成分
公开(公告)号：US4378990A
公开(公告)日：1983-04-05
申请号：US212679
申请日：1980-12-03
申请人： Keiji Endo , Tomomi Toriyama , Kisaku Mori
发明人： Keiji Endo , Tomomi Toriyama , Kisaku Mori
IPC分类号： A01N43/828 , A01N47/36 , A01N43/02 , A01N31/00
CPC分类号： A01N47/36
摘要： Herbicidal composition containing 5-(N-phenylcarbamoylamino)-1,2,3-thiadiazole and either a diphenyl ether of the formula ##STR1## or an acetanilide of the formula ##STR2## in which X is chlorine, methyl or trifluoromethyl, Y is hydrogen, lower alkoxy, ethoxyethoxyethoxy or methoxycarbonyl, n is 1 to 3, R.sub.1 and R.sub.2 are lower alkyl and R.sub.3 is lower alkoxy-lower alkyl. The active components display synergistic effectiveness as broad-spectrum herbicides for perennial as well as annual weeds in paddy fields. Suitable solid and liquid carrier materials for the composition include emulsifiers.
摘要翻译：含有5-（N-苯基氨基甲酰基氨基）-1,2,3-噻二唑和式“IMAGE”的二苯醚或式“IMAGE”的乙酰苯胺的除草组合物，其中X为氯，甲基或三氟甲基，Y为氢，低级烷氧基，乙氧基乙氧基乙氧基或甲氧基羰基，n为1至3，R 1和R 2为低级烷基，R 3为低级烷氧基 - 低级烷基。活性成分显示出作为广泛用于稻田多年生和一年生杂草的广谱除草剂的协同效应。用于组合物的合适的固体和液体载体材料包括乳化剂。

55. 发明授权

US08940514B2 Thioesterase and a method of producing fatty acids or lipids using the thioesterase 有权
标题翻译：硫酯酶和使用硫酯酶生产脂肪酸或脂质的方法
公开(公告)号：US08940514B2
公开(公告)日：2015-01-27
申请号：US13696147
申请日：2011-04-13
申请人： Takuto Tojo , Keiji Endo
发明人： Takuto Tojo , Keiji Endo
IPC分类号： C12N9/18 , A01H1/06 , C12N15/00 , C12N1/20 , C12N15/82 , C12N9/16 , C12P7/64
CPC分类号： C12N15/8247 , C12N9/16 , C12P7/6418 , C12P7/6436 , C12Y301/02014
摘要： A thioesterase comprising an amino acid sequence set forth in SEQ ID NO: 1, a thioesterase gene encoding the thioesterase, a transformant comprising the gene, and a method of producing fatty acids or lipids using the transformant.
摘要翻译：包含SEQ ID NO：1所示氨基酸序列的硫酯酶，编码硫酯酶的硫酯酶基因，包含该基因的转化体，以及使用该转化体产生脂肪酸或脂质的方法。

56. 发明授权

US08293516B2 Recombinant microorganism 有权
标题翻译：重组微生物
公开(公告)号：US08293516B2
公开(公告)日：2012-10-23
申请号：US12528039
申请日：2007-12-27
申请人： Keiji Endo , Katsutoshi Ara
发明人： Keiji Endo , Katsutoshi Ara
IPC分类号： C12N1/20 , C12P21/06
CPC分类号： C12N15/75 , C07K14/32 , C12P21/02
摘要： A recombinant microorganism is provided that has increased productivity of a protein or polypeptide of interest. A method for producing a protein of interest using the microorganism is also provided. The microorganism is prepared by inserting, in its genome, a transcription initiation regulatory region or both a transcription initiation regulatory region and a ribosome-binding site, upstream of a Bacillus subtilis prsA gene and by deleting or inactivating one or more of the abrB gene, dltA gene, dltB gene, dltC gene, dltD gene, dltE gene, or a gene (genes) corresponding thereto.
摘要翻译：提供了具有增加的感兴趣的蛋白质或多肽的生产力的重组微生物。还提供了使用该微生物生产感兴趣的蛋白质的方法。通过在其基因组中插入转录起始调节区或在枯草芽孢杆菌prsA基因的上游插入转录起始调节区和核糖体结合位点，以及通过缺失或失活一个或多个abrB基因， dltA基因，dltB基因，dltC基因，dltD基因，dltE基因或与其对应的基因（基因）。

57. 发明申请

US20090221055A1 Novel Bacillus Subtilis Mutant Strain 有权
标题翻译：新型枯草芽孢杆菌突变株
公开(公告)号：US20090221055A1
公开(公告)日：2009-09-03
申请号：US12083539
申请日：2006-09-25
申请人： Ryosuke Kadoya , Keiji Endo , Masatoshi Tohata , Katsutoshi Ara , Naotake Ogasawara
发明人： Ryosuke Kadoya , Keiji Endo , Masatoshi Tohata , Katsutoshi Ara , Naotake Ogasawara
IPC分类号： C12N1/21
CPC分类号： C12N15/75 , C12N9/2417 , C12N9/54 , C12P21/02
摘要： Novel Bacillus subtilis mutant strains having good productivity of various enzymes are provided through extensive analysis of strains that are derived from Bacillus subtilis via gene disruption. The Bacillus subtilis mutant strains according to the present invention have genomic structures prepared by deletion of regions listed in the columns for deficient regions. Each of these Bacillus subtilis mutant strains exerts significantly improved secretory productivity of a protein when a gene encoding such a secretory target protein is introduced so that it can be expressed, compared with a case in which the same gene is introduced into a wild-type strain.
摘要翻译：通过对通过基因破坏衍生自枯草芽孢杆菌的菌株的广泛分析，提供具有各种酶的良好生产力的新型枯草芽孢杆菌突变菌株。根据本发明的枯草芽孢杆菌突变菌株具有通过缺失缺陷区列中列出的区域而制备的基因组结构。当与将相同基因导入野生型菌株的情况相比，当引入编码这种分泌型靶蛋白的基因以使其能够被表达时，这些枯草芽孢杆菌突变菌株中的每一种都能显着提高蛋白质的分泌生产力。

58. 发明申请

US20090081726A1 Recombinant Microorganism 有权
标题翻译：重组微生物
公开(公告)号：US20090081726A1
公开(公告)日：2009-03-26
申请号：US11722162
申请日：2005-12-20
申请人： Takeko Kodama , Keiji Endo , Katsuya Ozaki , Junichi Sekiguchi
发明人： Takeko Kodama , Keiji Endo , Katsuya Ozaki , Junichi Sekiguchi
IPC分类号： C12P21/04 , C12N1/21
CPC分类号： C12P21/02 , C07K14/32 , C12N9/54 , C12N15/75
摘要： A host microorganism capable of increasing productivity of a protein or polypeptide, a recombinant microorganism obtained by introducing a gene encoding a protein or polypeptide into the host microorganism, and a method for producing a protein or polypeptide using the recombinant microorganism are provided.Also provided is a recombinant microorganism obtained by introducing into a host microorganism a gene encoding a heterologous protein or polypeptide, wherein in said host microorganism the Bacillus subtilis aprX gene or a gene corresponding to the aprX gene has been deleted or knocked out, and a method for producing a protein or polypeptide using the recombinant microorganism.
摘要翻译：提供能够提高蛋白质或多肽的生产力的宿主微生物，通过将编码蛋白质或多肽的基因导入宿主微生物而获得的重组微生物，以及使用该重组微生物产生蛋白质或多肽的方法。还提供了通过向宿主微生物中导入编码异源蛋白质或多肽的基因而获得的重组微生物，其中在所述宿主微生物中，枯草芽孢杆菌aprX基因或与aprX基因相对应的基因已被缺失或敲除，用于使用重组微生物产生蛋白质或多肽。

59. 发明申请

US20090029417A1 Recombinant Microorganism 有权
标题翻译：重组微生物
公开(公告)号：US20090029417A1
公开(公告)日：2009-01-29
申请号：US12279271
申请日：2007-02-15
申请人： Keiji Endo , Shenghao Liu , Katsutoshi Ara
发明人： Keiji Endo , Shenghao Liu , Katsutoshi Ara
IPC分类号： C12P21/00 , C12N1/21
CPC分类号： C12N9/2437
摘要： The object of the invention is to provide a microorganism which enhances productivity of cellulase and is useful for industrial production of cellulase, and to provide a method for producing cellulase by use of the microorganism. The present invention provides a recombinant microorganism produced by transferring a gene encoding cellulase to a parental microorganism which has been genetically modified so as to overexpress a Bacillus subtilis secY gene or a gene corresponding thereto.
摘要翻译：本发明的目的是提供一种提高纤维素酶生产率并可用于纤维素酶的工业生产的微生物，并提供使用微生物生产纤维素酶的方法。本发明提供了一种通过将编码纤维素酶的基因转移到已被遗传修饰以使其过表达枯草芽孢杆菌SecY基因或相应基因的亲本微生物而产生的重组微生物。

60. 发明申请

US20070197390A1 Difluoromethanesulfonyl anilide derivatives useful as herbicides 失效
标题翻译：用作除草剂的二氟甲磺酰苯胺衍生物
公开(公告)号：US20070197390A1
公开(公告)日：2007-08-23
申请号：US10594570
申请日：2005-03-19
申请人： Koichi Araki , Sachio Kudo , Yoshitaka Sato , Keiji Endo , Shinichi Shirakura , Shin Nakamura
发明人： Koichi Araki , Sachio Kudo , Yoshitaka Sato , Keiji Endo , Shinichi Shirakura , Shin Nakamura
IPC分类号： A01N43/64
CPC分类号： C07D239/52
摘要： Novel difluoromethanesulfonamide derivatives of the formula (I) Difluoromethanesulfonamide derivatives represented by the formula wherein X represents halogen, Y represents CH or N, R1 represents hydrogen, and R2 represents hydrogen or hydroxy, or R1 and R2 together may form C=O with the carbon atom to which they are bonded, and their use as herbicide for paddy field weeds.
摘要翻译：式（I）的新型二氟甲磺酰胺衍生物由下式表示的二氟甲磺酰胺衍生物其中X表示卤素，Y表示CH或N，R 1表示氢，R 2表示氢或羟基或R 1和R 2一起可以与它们所键合的碳原子一起形成C = O，以及它们作为稻田杂草除草剂的用途。

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