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    • 55. 发明公开
    • 형질 전환 세포주와 백반증 마우스를 이용한 백모 방지물질 스크리닝 방법 및 그 백모 방지 물질을 함유하는백모방지용 조성물
    • 使用稳定的细胞系和加速的VITILIGO小鼠筛选抗灰霉素成分的方法,以及含有抗灰白色成分的组合物。
    • KR1020090008866A
    • 2009-01-22
    • KR1020070072182
    • 2007-07-19
    • (주)아모레퍼시픽
    • 민대진박녹현박원석황재성장이섭
    • C12N15/12C12N15/64
    • A screening method of anti-gray hair ingredient is provided to improve accuracy of screening, and reduce the time and expenses to be required for the screening, thereby efficiently preventing gray hair. The screening method of anti-gray hair ingredient comprises the steps of: treating the transformed cell line with the candidate anti-gray hair ingredient, and culturing the treated cell line in the culture medium; measuring the production amount of GLuc(Gaussia luciferase) gene existing in the culture medium; removing hairs from the back of vitiligo mouse and spreading the candidate anti-gray hair ingredient on the hair-removed back of mouse; and measuring the melanin amount in the new born hair from the back of vitiligo mouse, wherein the transformed cell line is produced by transforming a host cell line with an expression vector pMITF-GLuc containing microphtalmia-associated factor(MITF) promoter, GLuc gene, NeoR(neomycin resistant) gene and AmpR(ampicilin resistant) gene.
    • 提供防灰发成分的筛选方法,以提高筛选的准确性,减少筛选所需的时间和费用,从而有效防止灰发。 抗灰发成分的筛选方法包括以下步骤:用候选抗灰发成分处理转化的细胞系,并培养培养基中经处理的细胞系; 测定存在于培养基中的GLuc(高斯萤光素酶)基因的产生量; 从白癜风小鼠背部除去毛发,并将候选抗灰白色头发成分分散在小鼠的头发去除背部上; 并测量来自白癜风小鼠背部的新生毛发中的黑色素量,其中转化细胞株是通过用含有微小相关因子(MITF)启动子,GLuc基因的表达载体pMITF-GLuc转化宿主细胞系产生的, NeoR(新霉素抗性)基因和AmpR(氨苄青霉素抗性)基因。