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51. 发明申请

WO1983003306A1 METHOD AND COMPOSITION FOR FREE LIGAND ASSAYS 审中-公开
标题翻译：免费配对测定的方法和组合
公开(公告)号：WO1983003306A1
公开(公告)日：1983-09-29
申请号：PCT/GB1983000078
申请日：1983-03-17
申请人： EKINS, Roger, Philip
IPC分类号： G01N33/54
CPC分类号： G01N33/544 , G01N33/53 , G01N33/78
摘要： A method for determining the amount of free ligand in a test sample where the ligand is also present bound to one or more endogenous receptors, comprising combining the test sample, ligand receptor and unlabelled, differential binding ligand analogue, incubating to permit the free ligand and unlabelled, differential binding ligand analogue to compete for the ligand receptor separating the ligand analogue, determining the amount of ligand receptor bound to the ligand or to the ligand analogue, and correlating the amount of bound ligand receptor to the amount of free ligand present in the test sample.
摘要翻译：用于测定测试样品中还存在配体的游离配体的量的方法与一种或多种内源性受体结合，包括组合测试样品，配体受体和未标记的差异结合配体类似物，孵育以允许游离配体和未标记的差异结合配体类似物以竞争分配配体类似物的配体受体，确定与配体或配体类似物结合的配体受体的量，并将结合的配体受体的量与存在于配体中的游离配体的量相关联测试样品。

52. 发明申请

WO1988002489A1 METHOD OF DETERMINING A BIOLOGICAL SUBSTANCE INVOLVING LABELLING WITH A METAL CHELATE 审中-公开
标题翻译：确定涉及金属螯合物的标签的生物物质的方法
公开(公告)号：WO1988002489A1
公开(公告)日：1988-04-07
申请号：PCT/GB1987000663
申请日：1987-09-22
申请人： EKINS, Roger, Philip , DAKUBU, Salifu
发明人： EKINS, Roger, Philip
IPC分类号： G01N33/533
CPC分类号： G01N33/533 , G01N33/582 , G01N2458/40
摘要： A biological substance is determined with the use of a marker comprising a lanthanide or other metal ion coupled to the biological substance by means of a chelate-forming compound. The chelate complex is dissociated by reduction of the ambient pH to a sufficiently low level to liberate the metal ion and the metal ion is then chelated with a separate chelating chromophore with an increase in the pH to a value closer to the optimum for determination of the metal-chelated chromophore by fluorescence spectroscopy, luminometry, colourometry or the like.
摘要翻译：使用包含镧系元素或通过形成螯合物的化合物与生物物质结合的其它金属离子的标记物来测定生物物质。通过将环境pH降低到足够低的水平以释放金属离子，螯合络合物被解离，然后金属离子与单独的螯合发色团螯合，随着pH的增加，其值越来越接近用于测定金属螯合发色团，通过荧光光谱法，发光测定法，色谱法等测定。

53. 发明申请

WO1984001031A1 MEASUREMENT OF ANALYTE CONCENTRATION 审中-公开
标题翻译：分析浓度的测量
公开(公告)号：WO1984001031A1
公开(公告)日：1984-03-15
申请号：PCT/GB1983000210
申请日：1983-08-26
申请人： EKINS, Roger, Philip
IPC分类号： G01N33/54
CPC分类号： G01N33/54306 , G01N33/74
摘要： The concentration of an analyte, such as a hormone or other biologically active material, in a fluid, especially a body fluid, is measured by contacting the fluid with a trace amount of a binding agent, such as an antibody, specific for the analyte, determining a figure representative of the proportional occupancy of binding sites on the binding agent and estimating from that figure the analyte concentration. Provided that the amount of binding agent is sufficiently small that its introduction has no significant effect on the total free analyte concentration, the proportional occupancy of binding sites is independent of the volume of the fluid and hence it is not necessary to measure accurately beforehand the volume of the fluid or fluid sample being tested. It is therefore possible to design a concentration-measuring device for insertion into a body fluid of a living creature for in situ measurement of concentration.
摘要翻译：流体，特别是体液中的分析物例如激素或其他生物活性物质的浓度通过使流体与痕量的分析物特异的结合剂如抗体接触来测量，确定表示结合剂上结合位点的比例占有率的图，并从该图估计分析物浓度。如果粘合剂的量足够小，使其引入对总的游离分析物浓度没有显着影响，则结合位置的比例占据与流体的体积无关，因此不需要预先准确地测量体积的待测流体或流体样品。因此，可以设计用于插入活体的体液中的浓度测定装置，以进行现场浓度测定。

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