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41. 发明授权

US4416812A Method of preparing tissue thromboplastin 失效
标题翻译：制备组织凝血活酶的方法
公开(公告)号：US4416812A
公开(公告)日：1983-11-22
申请号：US446088
申请日：1982-12-01
申请人： Udo Becker , Eugen Schaich , Manfred Weigert
发明人： Udo Becker , Eugen Schaich , Manfred Weigert
IPC分类号： G01N33/86 , A61K35/30 , A61K38/00 , A61K38/16 , A61K38/46 , C07K14/745 , C12Q1/56 , G01N33/50 , C07G7/00 , C07G7/026
CPC分类号： C12Q1/56 , C07K14/745 , A61K38/00 , G01N2333/745 , Y10S530/839
摘要： A tissue thromboplastin preparation which is sensitive to coagulation factor VII, is prepared from acetone dry powder mammalian tissue, by extraction with a salt solution containing 1 to 20 mmol/1 of calcium ions and, if desired, a surface active agent, after which the extract maybe dried.
摘要翻译：对凝血因子VII敏感的组织凝血激酶制剂，由丙酮干粉末哺乳动物组织制备，用含有1〜20mmol / l钙离子的盐溶液和需要时的表面活性剂提取，之后提取物可能会干燥。

42. 发明授权

US4254535A Method and an apparatus for making basic material for the production of a biologically active preparation 失效
标题翻译：用于生产生物活性制剂的基本材料的方法和装置
公开(公告)号：US4254535A
公开(公告)日：1981-03-10
申请号：US61071
申请日：1979-07-26
申请人： Ernst L. Pedersen , Erik Balslev
发明人： Ernst L. Pedersen , Erik Balslev
IPC分类号： A22B5/18 , C07G7/026 , A22C17/06
CPC分类号： A22B5/18
摘要： A method and apparatus for making basic or starting material for the production of a biologically active preparation such as pepsin and/or mucin from stomachs of swines or hogs. The starting material is made by mechanically separating mucous cells from their supporting membrane at the fundus regions of the stomachs without substantially damaging said membrane, the mucous cells may, for example, be separated by cutting, abrading, or scraping action. The stomachs are preferably suspended at their thicker edge portions while they are treated by one or more pairs of oppositely rotating rollers having scraping edges thereon such as flexible fingers or knobs. The invention also relates to a basic material produced by the said method and substantially consisting of mucous cells separated from the membranes at the fundus region of stomachs of swines or hogs.
摘要翻译：一种用于生产生物活性制剂如猪或猪猪胃蛋白酶和/或粘蛋白的碱性或起始物质的方法和装置。起始材料通过在胃的眼底区域从其支撑膜上机械分离粘液细胞而基本上不损伤所述膜而制成，例如可以通过切割，研磨或刮擦作用分离粘液细胞。优选地将胃悬挂在其较厚的边缘部分，同时通过一对或多对相对旋转的辊在其上具有刮擦边缘（例如柔性指状物或旋钮）来对其进行处理。本发明还涉及通过所述方法产生的基本材料，并且基本上由在猪或猪的胃的眼底区域从膜分离的粘液细胞组成。

43. 发明授权

US4205129A Method for purifying galactosyltransferase isoenzymes and product 失效
标题翻译：半乳糖转移酶同工酶和产物的纯化方法
公开(公告)号：US4205129A
公开(公告)日：1980-05-27
申请号：US017288
申请日：1979-03-05
申请人： Daniel K. Podolsky , Milton M. Weiser
发明人： Daniel K. Podolsky , Milton M. Weiser
IPC分类号： C12N9/10 , C07G7/026
CPC分类号： C12N9/1051 , Y10S435/815
摘要： Pure galactosyltransferase isoenzyme GT-II having a molecular weight of about 71,000 is obtained from malignant cells or tumors of animals and humans. The GT-II is isolated by subjecting a body fluid or extract of the malignant cells or tumors containing the isoenzyme and GT-I galactosyltransferase isoenzyme to serial column separation steps and utilizing materials in the column which resemble cofactors for galactosyltransferase GT-II.
摘要翻译：具有约71,000分子量的纯半乳糖基转移酶同工酶GT-II是从恶性细胞或动物和人的肿瘤得到的。通过使体液或含有同工酶和GT-1半乳糖基转移酶同工酶的肿瘤的体液或提取物进行连续柱分离步骤并利用类似于用于半乳糖转移酶GT-II的辅因子的柱中的材料来分离GT-II。

44. 发明授权

US4178212A Stabilized thymidine phosphorylase formulation 失效
标题翻译：稳定的胸苷磷酸化酶制剂
公开(公告)号：US4178212A
公开(公告)日：1979-12-11
申请号：US890781
申请日：1978-03-27
申请人： Thomas A. Krenitsky , Stanley R. M. Bushby
发明人： Thomas A. Krenitsky , Stanley R. M. Bushby
IPC分类号： C12N9/10 , C12N9/96 , C12Q1/18 , C07G7/026
CPC分类号： C12Q1/18 , C12N9/1077 , C12N9/96
摘要： A bacterial culture medium used in testing for the susceptibility of bacteria to anti-folate, anti-bacterial agents is substantially improved for this use by the incorporation therein of purified thymidine phosphorylase of bacterial origin.
摘要翻译：用于测试细菌对抗叶酸，抗细菌剂的敏感性的细菌培养基通过掺入细菌来源的纯化的胸苷磷酸化酶而大大改善了这一用途。

45. 发明授权

US4141887A Process and apparatus for the production of sterile filtered blood clotting factors 失效
标题翻译：用于生产无菌过滤凝血因子的方法和装置
公开(公告)号：US4141887A
公开(公告)日：1979-02-27
申请号：US800776
申请日：1977-05-26
申请人： Arnold Seufert
发明人： Arnold Seufert
IPC分类号： A61K35/14 , A61K38/00 , A61K38/16 , A61L2/00 , A61L2/02 , C07K14/755 , A23J1/06 , C07G7/026
CPC分类号： C07K14/755 , A61L2/0017 , A61L2/022 , A61K38/00 , Y10S530/83
摘要： A process for the manufacture of sterile filtered blood-clotting products includes the steps of extracting blood from at least one donor, physically separating the blood to obtain substantially purified plasma free of cellular components, deep freezing the purified plasma at a temperature below about minus 22.degree. C. to preserve Factor VIII, thawing the plasma to produce a cryo-precipitate, enriching the cryo-precipitate by physical treatment and physically separating the cryo-precipitate from the thawed plasma. The cryo-precipitate is then dissolved in a buffer and is sterile filtered by means of a static filtration with an autoclaved filtration device at a pressure difference of less than 0.5 atm. An apparatus for carrying out the process is also provided.
摘要翻译：用于制造无菌过滤血液凝固产物的方法包括以下步骤：从至少一个供体中提取血液，物理分离血液以获得基本上纯化的无细胞成分的血浆，在低于约22℃的温度下将纯化的血浆深度冷冻保存因子VIII，解冻等离子体以产生冷冻沉淀，通过物理处理富集冷冻沉淀物，并从解冻的血浆物理分离冷冻沉淀物。然后将冷冻沉淀物溶解在缓冲液中，并通过在压力差小于0.5atm下的高压灭菌过滤装置静态过滤进行无菌过滤。还提供了一种用于执行该过程的装置。

46. 发明授权

US4082612A Plasminogen activator complex 失效
标题翻译： PLASMINOGEN激活剂复合物
公开(公告)号：US4082612A
公开(公告)日：1978-04-04
申请号：US726142
申请日：1976-09-24
申请人： Kenneth C. Robbins , Louis Summaria
发明人： Kenneth C. Robbins , Louis Summaria
IPC分类号： A61K35/14 , A61K38/00 , A61K38/16 , A61K38/48 , A61P7/02 , C12N9/68 , C07G7/026 , A61K37/48
CPC分类号： C12N9/6435 , C12Y304/21007 , A61K38/00
摘要： A functionally active fraction of plasmin is provided which is capable of forming with streptokinase a complex with plasminogen activator activity.The fraction is made by reducing the disulfide bonds of plasmin molecules while inhibiting the serine protease activity of the plasmin and separating the light (B) chain fraction from the reaction product.

47. 发明授权

US4065355A Purification of deoxyribonuclease 失效
标题翻译：脱氧核糖核酸酶的纯化
公开(公告)号：US4065355A
公开(公告)日：1977-12-27
申请号：US730739
申请日：1976-10-08
申请人： Boen Tie Khouw , Johan Peter Kesler
发明人： Boen Tie Khouw , Johan Peter Kesler
IPC分类号： C12N9/22 , C07G7/02 , C07G7/026
CPC分类号： C12N9/22 , Y10S435/815
摘要： Highly purified deoxyribonuclease is obtained by a simple process which comprises adsorption of deoxyribonuclease from a crude or partially purified solution of the enzyme on a column of concanavalin A-agarose, followed by elution of the adsorbed deoxyribonuclease with a carbohydrate solution and recovery of the deoxyribonuclease in solid form from such solution.
摘要翻译：通过简单的方法获得高度纯化的脱氧核糖核酸酶，其包括将粗酶或部分纯化的酶溶液脱氧核糖核酸酶吸附在刀豆素A-琼脂糖柱上，随后用碳水化合物溶液洗脱吸附的脱氧核糖核酸酶，并回收脱氧核糖核酸酶这种溶液的固体形式。

48. 发明授权

US4048014A Method for obtaining urokinase 失效
标题翻译：获得尿激酶的方法
公开(公告)号：US4048014A
公开(公告)日：1977-09-13
申请号：US702900
申请日：1976-07-06
申请人： Masaharu Urakawa , Hiroshi Sumiyama
发明人： Masaharu Urakawa , Hiroshi Sumiyama
IPC分类号： C12N9/72 , C07G7/026
CPC分类号： C12N9/6462 , C12Y304/21073 , Y10S435/815
摘要： A method for obtaining urokinase which comprises steps of contacting a partially purified urokinase-containing solution with diatomaceous earth at a pH range of 4.0 - 8.0, washing said diatomaceous earth adsorbing urokinase thereon with a buffer solution of pH 6.5 - 9.0 and eluting the urokinase from the adsorbate.
摘要翻译：一种获得尿激酶的方法，包括以下步骤：在4.0-8.0的pH范围内使部分纯化的含尿激酶的溶液与硅藻土接触，用pH 6.5-9.0的缓冲溶液洗涤所述硅藻土吸附尿激酶，并将尿激酶从被吸附物。

49. 发明授权

US4038141A Methods for extracting and purifying kallidinogenase 失效
标题翻译：提取和净化KALLIDINOGENASE的方法
公开(公告)号：US4038141A
公开(公告)日：1977-07-26
申请号：US674645
申请日：1976-04-07
申请人： Kiyochika Tokuyasu , Takeshi Yokobori , Chizuko Nakahara
发明人： Kiyochika Tokuyasu , Takeshi Yokobori , Chizuko Nakahara
IPC分类号： C12N9/64 , C07G7/026 , C12D13/10
CPC分类号： C12N9/6445 , Y10S435/815
摘要： A method for extracting and collecting kallidinogenase (EC 3.4.4.21 substance) which comprises extracting the pancreas of a mammal with water in the presence of a salt at a temperature of about 45.degree. to about 60.degree. C. and a pH of about 5 to about 8, wherein the extraction is carried out in the presence of a water-soluble salt of magnesium with an acid, and a method for purifying kallidinogenase which comprises subjecting the aqueous phase of a kallidinogenase-containing aqueous extract obtained from the pancreas of a mammal to an adsorption-elution treatment using an anion-exchange resin, wherein said aqueous phase of the kallidinogenase-containing aqueous extract is brought into contact with a gel-type weakly basic anion-exchange resin to cause the kallidinogenase fraction to be adsorbed to said resin, and the kallidinogenase is eluted with an aqueous solution of an ammonium salt of a weak acid, thereby to collect the kallidinogenase.

50. 发明授权

US3994782A Methods for extracting and purifying kallidinogenase 失效
标题翻译：提取和纯化杀鼠肽素酶的方法
公开(公告)号：US3994782A
公开(公告)日：1976-11-30
申请号：US638042
申请日：1975-12-05
申请人： Kiyochika Tokuyasu , Takeshi Yokobori , Chizuko Nakahara
发明人： Kiyochika Tokuyasu , Takeshi Yokobori , Chizuko Nakahara
IPC分类号： C12N9/64 , C07G7/026 , A61K37/48
CPC分类号： C12N9/6445 , Y10S435/814 , Y10S435/815
摘要： A method for extracting and collecting kallidinogenase (EC 3.4.4.21 substance) which comprises extracting the pancreas of a mammal with water in the presence of a salt at a temperature of about 45.degree. to about 60.degree. C. and a pH of about 5 to about 8, wherein the extraction is carried out in the presence of a water-soluble salt of magnesium with an acid, and a method for purifying kallidinogenase which comprises subjecting the aqueous phase of a kallidinogenase-containing aqueous extract obtained from the pancreas of a mammal to an adsorption-elution treatment using an anion-exchange resin, wherein said aqueous phase of the kallidinogenase-containing aqueous extract is brought into contact with a gel-type weakly basic anion-exchange resin to cause the kallidinogenase fraction to be adsorbed to said resin, and the kallidinogenase is eluted with an aqueous solution of an ammonium salt of a weak acid, thereby to collect the kallidinogenase.
摘要翻译：一种提取和收集杀鼠肽素酶（EC 3.4.4.21物质）的方法，其包括在盐存在下，在约45℃至约60℃的温度和约5至约5℃的温度下用水萃取哺乳动物的胰腺，约8，其中所述提取在镁的水溶性盐与酸的存在下进行，以及纯化杀青霉素酶的方法，该方法包括使水相从哺乳动物的胰腺获得的含有杀线索杆菌素酶的水提取物使用阴离子交换树脂进行吸附洗脱处理，其中使所述含有杀线虫素原的水提取物的所述水相与凝胶型弱碱性阴离子交换树脂接触，以使所述蓝霉素酶部分被所述树脂吸附，并用弱酸的铵盐的水溶液洗脱苍耳蛋白原酶，从而收集苍白球素原酶。

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