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    • 47. 发明申请
    • COMPOSITIONS AND METHODS RELATING TO THE PRODUCTION, ISOLATION, AND MODIFICATION OF GAS VESICLES
    • 关于气体产生,分离和改造的组合物和方法
    • WO1998021311A1
    • 1998-05-22
    • PCT/US1997021192
    • 1997-11-12
    • THE UNIVERSITY OF AKRON
    • THE UNIVERSITY OF AKRONJU, Lu-KwangSUNDARARAJAN, AnandKASHYAP, Sunil
    • C12N01/12
    • C12N1/00C12M29/20C12M33/00C12N1/02C12N1/12C12N1/20Y10S435/832
    • A method for delivering a gas to a site comprising placing cells having gas vesicles under conditions that induce the cells to float to a surface of an aqueous medium, harvesting the cells from the surface of the medium, lysing the cells, separating the gas vesicles from the lysed cells, crosslinking the gas vesicles with a crosslinking agent, loading a gas into the gas vesicles, and placing the gas vesicles such that the gas is delivered to the site. Harvesting gas-vesicle-containing cells is achieved by placing the cells under conditions that induce the cells to rise to the surface of an aqueous medium - such as darkness, exponential growth stage, flocculation, or dissolved gas flotation - then collecting the cells from the surface of the medium. Gas vesicles are isolated by lysing the cells and separating the gas vesicles from the lysate. Once the gas vesicles are isolated, they can be modified, such as by crosslinking with glutaraldehyde.
    • 一种用于将气体输送到位置的方法,包括在诱导细胞漂浮到水性介质的表面的条件下放置具有气泡的细胞,从培养基表面收获细胞,裂解细胞,将气泡从 裂解的细胞,用交联剂交联气泡,将气体加载到气泡中,并放置气泡,使得气体被输送到现场。 通过将细胞置于诱导细胞升至水性介质表面的条件(例如黑暗,指数生长阶段,絮凝或溶解气体浮选)下,将细胞置于收集含气囊泡细胞中,然后从 介质表面。 通过裂解细胞并从溶胞产物中分离出气体囊泡来分离气泡。 一旦气体囊泡被分离,它们可以被改变,例如通过与戊二醛交联。