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41. 发明申请

US20050233360A1 Nucleic acid probes and methods to detect and/or quantify nucleic acid analytes 审中-公开
公开(公告)号：US20050233360A1
公开(公告)日：2005-10-20
申请号：US11083210
申请日：2005-03-16
申请人： Martin Davies , Ian Bruce , Andreas Wolter
发明人： Martin Davies , Ian Bruce , Andreas Wolter
IPC分类号： C07H21/00 , C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6818 , C07H21/00 , C12Q1/686 , C12Q2565/107 , C12Q2565/1015 , C12Q2563/173 , C12Q2525/313 , C12Q2527/119 , C12Q2525/113 , C12Q2561/113
摘要： The invention comprises novel methods and strategies to detect and/or quantify nucleic acid analytes. The methods involve nucleic acid probes with covalently conjugated dyes, which are attached either at adjacent nucleotides or at the same nucleotide of the probe and novel linker molecules to attach the dyes to the probes. The nucleic acid probes generate a fluorescent signal upon hybridization to complementary nucleic acids based on the interaction of one of the attached dyes, which is either an intercalator or a DNA groove binder, with the formed double stranded DNA. The methods can be applied to a variety of applications including homogeneous assays, real-time PCR monitoring, transcription assays, expression analysis on nucleic acid microarrays and other microarray applications such as genotyping (SNP analysis). The methods further include pH-sensitive nucleic acid probes that provide switchable fluorescence signals that are triggered by a change in the pH of the medium.

42. 发明授权

US4563187A Composition for cleaning textile surfaces 失效
标题翻译：用于清洁纺织品表面的成分
公开(公告)号：US4563187A
公开(公告)日：1986-01-07
申请号：US579859
申请日：1984-02-13
申请人： Otto Mesmer , Wolfgang Polligkeit , Wolfgang Troger , Andreas Wolter
发明人： Otto Mesmer , Wolfgang Polligkeit , Wolfgang Troger , Andreas Wolter
IPC分类号： C11D3/00 , C11D3/37 , C11D17/00 , C11D17/04 , C11D17/06
CPC分类号： C11D3/3703 , C11D17/00 , C11D17/041 , C11D3/0031
摘要： A cleaning means is disclosed for textile surfaces, particularly textile floor coverings, containing a powdery, porous carrier material based upon urea-formaldehyde-resin foam which is enriched with cleaning agent, whereby the tenside-containing water adhered within the carrier material from the foaming-up process is stored completely homogeneously in the carrier material, and forms together with the cleaning agent the prepared product. It is suggested for lowering the free formaldehyde content, to add to the tenside-containing water adhering within the carrier material, a salt of sulfurous acid in an effective portion, relative to the anion of the acid, from 0.01-2% by weight. This strongly reduces the portion of free formaldehyde and adjusts the pH-value of the cleaning means between 6 and 9.
摘要翻译：公开了用于织物表面，特别是纺织品地板覆盖物的清洁装置，其包含基于富含清洁剂的脲甲醛树脂泡沫的粉末状多孔载体材料，由此将载体材料中的含表面活性剂的水从发泡该过程完全均匀地储存在载体材料中，并与清洁剂一起形成所制备的产品。建议降低游离甲醛含量，添加粘附在载体材料中的含表面活性剂的水，相对于酸阴离子的有效部分中的亚硫酸盐为0.01-2重量％。这大大减少了游离甲醛的部分，并调节了6到9之间的清洁装置的pH值。

43. 发明申请

US20080064867A1 PROCESS FOR THE SYNTHESIS OF OLIGONUCLEOTIDES 失效
标题翻译：合成寡核苷酸的方法
公开(公告)号：US20080064867A1
公开(公告)日：2008-03-13
申请号：US11853662
申请日：2007-09-11
申请人： Michael Leuck , Andreas Wolter , Alfred Stumpe
发明人： Michael Leuck , Andreas Wolter , Alfred Stumpe
IPC分类号： C07H21/00
CPC分类号： C07H21/00 , Y02P20/55
摘要： The present invention discloses novel methods for the synthesis of oligonucleotides with nucleoside phosphoramidites on solid supports. The methods comprise the stepwise chain assembly of oligonucleotides on supports with 5′-acyl phosphoramidites. The synthesis cycles consist of a front end deprotection step which is conducted with a solution of a primary amine or a phenolate, a phosphoramidite coupling step with a 5′-acyl nucleoside phosphoramidite in the presence of an activator, a phosphite oxidation step and an optional capping step. The novel methods improve the quality of synthetic oligonucleotides due to the irreversibility of the front end deprotection step, which prevents the formation of deletion sequences, and due to the avoidance of acidic reagents in the synthesis cycles, which prevent the formation of depurination side products. The invention further discloses novel nucleoside phosphoramidite compositions wherein the phosphoramidites carry acyl front end protective groups which are cleavable with primary amines or phenolates. The invention is applicable to the synthesis of oligodeoxyribonucleotides, oligoribonucleotides and oligonucleotides with modifications in their sugar or phosphate groups.
摘要翻译：本发明公开了在固体支持物上合成具有核苷亚磷酰胺的寡核苷酸的新方法。所述方法包括在载体上用5'-酰基亚磷酰胺逐步链组装寡核苷酸。合成循环由在活化剂存在下用伯胺或酚盐溶液，亚磷酰胺偶联步骤与5'-酰基核苷亚磷酰胺进行的前端去保护步骤，亚磷酸酯氧化步骤和任选的封顶步新方法由于前端脱保护步骤的不可逆性而改善了合成寡核苷酸的质量，这防止了缺失序列的形成，并且由于避免了在合成循环中的酸性试剂，从而防止了形成脱嘌呤产物。本发明进一步公开了新的核苷亚磷酰胺组合物，其中亚磷酰胺携带可与伯胺或酚盐切割的酰基前端保护基。本发明适用于寡脱氧核糖核苷酸，寡核糖核苷酸和其糖或磷酸基团修饰的寡核苷酸的合成。

44. 发明授权

US07329515B2 Solid support for the synthesis of 3′-amino oligonucleotides 有权
标题翻译：支持3'-氨基寡核苷酸的合成
公开(公告)号：US07329515B2
公开(公告)日：2008-02-12
申请号：US10830484
申请日：2004-04-21
申请人： Michael Leuck , Andreas Wolter
发明人： Michael Leuck , Andreas Wolter
IPC分类号： C12P19/34 , C07H21/02 , C07H21/04 , C07C229/56
CPC分类号： C07H21/02 , C07H21/04
摘要： The present invention discloses novel methods and solid supports for the synthesis of 3′-amino oligonucleotides. The novel supports are based on an unsubstituted or ring-substituted hydroxymethylbenzoyl linker element wherein the hydroxymethyl group is esterified to a solid phase bound carboxylic acid and the carbonyl group is linked to an amino alcohol as an amide. Oligonucleotides are conveniently synthesized on the novel supports with no modifications in the standard phosphoramidite synthesis scheme. The ester function of the support is cleaved under the alkaline deprotection conditions for oligonucleotides to provide a free hydroxymethyl group that aids in the release of the 3′-amino oligonucleotide products with a free amino group through neighbor group participation. The free amino group of the oligonucleotides is available for further conjugation reactions to haptens, reporter groups, surfaces or other small molecules or biomolecules. The methods provided are particularly mild, do not require any modifications in standard protocols for the synthesis and deprotection of oligonucleotides, provide the 3′-amino oligonucleotides free of side products and do not introduce chiral centers to the oligonucleotides.
摘要翻译：本发明公开了用于合成3'-氨基寡核苷酸的新方法和固体支持物。该新型载体是基于未取代的或环取代的羟甲基苯甲酰基接头元件，其中羟甲基被酯化成固相结合的羧酸，羰基连接到氨基醇作为酰胺。在新型载体上方便地合成寡核苷酸，在标准亚磷酰胺合成方案中没有修饰。在碱性脱保护条件下，对寡核苷酸的酯功能进行切割，提供游离羟甲基，有助于游离氨基的3'-氨基寡核苷酸产物通过邻群参与释放。寡核苷酸的游离氨基可用于进一步与半抗原，报告基团，表面或其他小分子或生物分子的缀合反应。所提供的方法特别温和，不需要对寡核苷酸的合成和去保护的标准方案进行任何修饰，提供不含副产物的3'-氨基寡核苷酸，并且不向寡核苷酸引入手性中心。

45. 发明申请

US20060214279A1 Contacting device, testing method and corresponding production method 有权
标题翻译：接触装置，试验方法及相应的生产方法
公开(公告)号：US20060214279A1
公开(公告)日：2006-09-28
申请号：US11374391
申请日：2006-03-13
申请人： Andreas Wolter , Jorg Zapf
发明人： Andreas Wolter , Jorg Zapf
IPC分类号： H01L23/48
CPC分类号： G01R1/0466 , G01R1/0483 , H01L2224/81385
摘要： A contacting device comprises a carrier device with a first surface, a plurality of first terminal regions on the first surface, at least one elastic elevation on the first surface, and a plurality of interconnects, each running from a respective of the first terminal regions to an upper side of the elastic elevation. The plurality of first terminal regions is configured so that signals of a tester device can be fed to the plurality of first terminal regions, the interconnects have first contact regions located at the upper side of the elastic elevation configured to be contacted electrically with corresponding second contact regions of an integrated circuit, and the first contact regions comprise first particles for roughening the surface of the first contact regions.
摘要翻译：接触装置包括具有第一表面的载体装置，在第一表面上的多个第一端子区域，在第一表面上的至少一个弹性高度，以及多个互连件，每个互连件从相应的第一端子区域延伸到弹性高度的上侧。多个第一端子区域被配置为使得测试器装置的信号可以被馈送到多个第一端子区域，互连具有位于弹性高度的上侧的第一接触区域，其被配置为与相应的第二接触件电接触区域，并且第一接触区域包括用于使第一接触区域的表面粗糙化的第一颗粒。

46. 发明授权

US4753746A Urea-formaldehyde foam detergent and method of manufacture using a thixotropic agent 失效
标题翻译：尿素 - 甲醛泡沫洗涤剂和使用触变剂的制造方法
公开(公告)号：US4753746A
公开(公告)日：1988-06-28
申请号：US943762
申请日：1986-12-19
申请人： Otto Mesmer , Wolfgang Polligkeit , Ernst-Uwe Schiffer , Wolfgang Troger , Andreas Wolter
发明人： Otto Mesmer , Wolfgang Polligkeit , Ernst-Uwe Schiffer , Wolfgang Troger , Andreas Wolter
IPC分类号： C11D3/00 , C11D3/37 , C11D17/04 , C08G12/12 , C08J9/40 , C09K3/22
CPC分类号： C11D3/3703 , C11D17/049 , C11D3/0031
摘要： The invention is directed to a detergent and a method of producing the same. The detergent is for textile surfaces and especially for textile floor coverings. The detergent includes a pulverized, porous carrier including a foamed, plastified urea-formaldehyde resin foam. The carrier material is enriched with detergent so that the finished product with respect to the carrier material has a weight per unit volume of 60 kg/m.sup.3 and an apparent density of 50 to 150 grams/liter; the granular size of the carrier material is between 0.01 and 12 mm; the surfactant containing water, which adheres to the carrier material, is stored in the carrier material in a completely homogeneous manner in a share of maximally 80% by weight referred to the weight of the carrier material; and, the detergent is produced from the mixture of the carrier material with a highly concentrated aqueous cleaning solution. The method includes the steps of foaming the plastified urea-formaldehyde resin foam; and, combining the free formaledhyde present during the manufacturing process prior to and/or during the foaming of the plastified urea-formaldehyde resin foam by adding formaldehyde binding substances and stabilizing substances to at least one of the solutions selected from the group consisting of the foaming agent solution and the resin solution.
摘要翻译：本发明涉及一种洗涤剂及其制造方法。洗涤剂用于织物表面，特别是用于纺织品地板覆盖物。洗涤剂包括粉碎的多孔载体，其包括发泡的，塑化的脲 - 甲醛树脂泡沫。载体材料富含洗涤剂，使成品相对于载体材料的每单位体积的重量为60kg / m 3，表观密度为50至150克/升; 载体材料的粒度为0.01〜12mm; 附着在载体材料上的含有表面活性剂的水以完全均匀的方式储存在载体材料中，最大量为载体材料重量的80％（重量）; 并且洗涤剂由载体材料与高度浓缩的水性清洗溶液的混合物制成。该方法包括使增塑的脲醛树脂发泡体发泡的步骤; 并且通过向至少一种选自以下的溶液中加入甲醛结合物质和稳定物质，在塑化的脲 - 甲醛树脂泡沫的发泡之前和/或期间，在制造过程中存在的游离醛缩合物，试剂溶液和树脂溶液。

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