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41. 发明申请

US20070024964A1 Fluorescence spectroscopic apparatus 有权
标题翻译：荧光光谱仪
公开(公告)号：US20070024964A1
公开(公告)日：2007-02-01
申请号：US11529017
申请日：2006-09-28
申请人： Kiyochika Ishibashi , Atsushi Miyawaki
发明人： Kiyochika Ishibashi , Atsushi Miyawaki
IPC分类号： G02B21/00
CPC分类号： G01N21/6408 , G01N21/6428 , G01N2021/6419 , G01N2021/6441
摘要： There is provided a fluorescence spectroscopic apparatus includes an exciting optical unit configured to irradiate the same sample area with a plurality of excitation lights of different wavelength bands, an optical unit configured to repeatedly guide fluorescences emitted by the sample in response to the respective excitation lights, to a detection unit, and a calculation unit configured to perform analysis on the basis of a comparison of output signals corresponding to the fluorescences from the detection unit, wherein the exciting optical unit includes an excitation light varying unit configured to intermittently vary the intensity of at least one excitation light.
摘要翻译：提供了一种荧光分光装置，包括：激发光学单元，被配置为用不同波长带的多个激发光照射相同的样本区域;光学单元，被配置为响应于各个激发光反复引导样品发射的荧光，检测单元和计算单元，其被配置为基于与来自检测单元的荧光相对应的输出信号的比较来执行分析，其中激发光学单元包括激励光改变单元，其被配置为间歇地改变在至少一个激发光。

42. 发明授权

US07126752B2 Illumination apparatus for microscope and image processing apparatus using the same 有权
标题翻译：用于显微镜的照明装置和使用其的图像处理装置
公开(公告)号：US07126752B2
公开(公告)日：2006-10-24
申请号：US10718884
申请日：2003-11-20
申请人： Atsushi Miyawaki , Takashi Fukano , Yasushi Aono
发明人： Atsushi Miyawaki , Takashi Fukano , Yasushi Aono
IPC分类号： G02B21/00 , G02B21/06
CPC分类号： G02B21/06 , G02B21/16 , G02B21/365
摘要： An illumination apparatus for a microscope and an image processing apparatus using the illumination apparatus include a light source, a semi-transmissive mirror splitting a light beam from the light source into two beams of the first and second irradiation light, two excitation filters selecting the wavelengths of the first and second irradiation light, a semi-transmissive mirror synthesizing individual beams of the first and second irradiation light whose wavelengths are selected, into a single beam, a dichroic mirror directing a light beam synthesized by the semi-transmissive mirror toward a specimen and transmitting light from the specimen, an objective lens, cameras imaging fluorescent light from the specimen after being separated into fluorescent light excited by the first and second wavelengths, and an image processing section processing fluorescent images formed by imaging elements.
摘要翻译：用于显微镜的照明装置和使用该照明装置的图像处理装置包括光源，将来自光源的光束分成两束第一和第二照射光的半透射镜，两个激发滤光器选择波长第一和第二照射光的第一和第二照射光的半透射镜将将波长选择的第一和第二照射光的各个光束合成为单个光束，将由半透射镜合成的光束引向样品的二向色镜以及透射来自所述样本的物体，物镜，将被分离成由所述第一和第二波长激发的荧光的荧光从所述样本成像的摄像机，以及处理由成像元件形成的荧光图像的图像处理部。

43. 发明授权

US07060869B2 Fluorescent protein sensors for detection of analytes 失效
公开(公告)号：US07060869B2
公开(公告)日：2006-06-13
申请号：US09554000
申请日：2000-04-20
申请人： Roger Y. Tsien , Atsushi Miyawaki
发明人： Roger Y. Tsien , Atsushi Miyawaki
IPC分类号： A61K67/027
CPC分类号： C07K14/43595 , A01K2217/05 , C07K2319/00 , G01N33/542
摘要： Fluorescent indicators including a binding protein moiety, a donor fluorescent protein moiety, and an acceptor fluorescent protein moiety are described. The binding protein moiety has an analyte-binding region which binds an analyte and causes the indicator to change conformation upon exposure to the analyte. The donor moiety and the acceptor moiety change position relative to each other when the analyte binds to the analyte-binding region. The donor moiety and the acceptor moiety exhibit fluorescence resonance energy transfer when the donor moiety is excited and the distance between the donor moiety and the acceptor moiety is small. The indicators can be used to measure analyte concentrations in samples, such as calcium ion concentrations in cells.

44. 发明授权

US10444124B2 Clarifying reagent for biological materials and use thereof 有权
公开(公告)号：US10444124B2
公开(公告)日：2019-10-15
申请号：US14118150
申请日：2012-05-18
申请人： Atsushi Miyawaki , Hiroshi Hama , Asako Sakaue
发明人： Atsushi Miyawaki , Hiroshi Hama , Asako Sakaue
IPC分类号： G01N1/30 , G01N33/50
摘要： A clearing reagent according to the present invention for making a biological material transparent is a solution containing: at a concentration of 1M or more and not more than 8.5M, at least one compound selected from the group consisting of urea and urea derivatives; and glycerol at a concentration of 25 (w/v) % or more and not more than 35 (w/v) %.

45. 发明授权

US09989518B2 Method for measuring autophagy 有权
公开(公告)号：US09989518B2
公开(公告)日：2018-06-05
申请号：US13389869
申请日：2010-10-08
申请人： Atsushi Miyawaki , Hiroyuki Katayama
发明人： Atsushi Miyawaki , Hiroyuki Katayama
IPC分类号： A61K31/7088 , G01N33/50 , G01N21/64 , G01N21/80
CPC分类号： G01N33/5005 , G01N21/6428 , G01N21/80 , G01N33/5035 , G01N2021/6419 , G01N2021/6421
摘要： This invention relates to a method for measuring autophagy in cells, comprising using, as a probe reagent, a single fluorescent protein, to measure a change in fluorescence properties of the fluorescent probe reagent depending on pH changes associated with autophagy, thereby determining the presence or activity of autophagy, wherein the single fluorescent protein is resistant to degrading enzyme activity in the lysosome or vacuole of the cell, it is not denatured or inactivated under acidic to neutral pH environment, and it is capable of changing excitation spectra or fluorescence spectra when located under the environments of acidic region and neutral region.

46. 发明授权

US08420781B2 Fluorescent protein 有权
标题翻译：荧光蛋白
公开(公告)号：US08420781B2
公开(公告)日：2013-04-16
申请号：US13196499
申请日：2011-08-02
申请人： Atsushi Miyawaki , Takako Kogure , Hiroshi Hama , Masataka Kinjo , Kenta Saito , Satoshi Karasawa , Toshio Araki
发明人： Atsushi Miyawaki , Takako Kogure , Hiroshi Hama , Masataka Kinjo , Kenta Saito , Satoshi Karasawa , Toshio Araki
IPC分类号： C07K1/00 , C08B37/08
CPC分类号： C07K14/37 , C07K14/43504 , G01N33/533
摘要： An object of the present invention is to provide a red or orange fluorescent protein, which is characterized in that the difference (stokes shift) between an excitation peak value (wavelength of maximum absorption) and a fluorescence peak value (wavelength of maximum fluorescence) is greatened, so that the maximum fluorescence can be obtained by the maximum excitation. The present invention provides a novel fluorescent protein monomerized by introducing a mutation into a florescent protein derived from Fungia sp., and a novel chromoprotein and fluorescent protein derived from Montipora. sp.
摘要翻译：本发明的目的是提供一种红色或橙色荧光蛋白，其特征在于，激发峰值（最大吸收波长）与荧光峰值（最大荧光的波长）之间的差异（斯托克斯偏移）为从而可以通过最大激发获得最大荧光。本发明提供了一种新颖的荧光蛋白，其通过将突变引入到来自真菌属的荧光蛋白中而单体化，以及衍生自Montipora的新型生色蛋白和荧光蛋白。 sp。

47. 发明申请

US20120288883A1 PROBE REAGENT FOR MEASUREMENT OF PROTEOLYTIC ACTIVITY 有权
标题翻译：用于测定活性的探针试剂
公开(公告)号：US20120288883A1
公开(公告)日：2012-11-15
申请号：US13574400
申请日：2011-01-21
申请人： Atsushi Miyawaki , Masahiko Hirano
发明人： Atsushi Miyawaki , Masahiko Hirano
IPC分类号： G01N21/76 , C12N5/10 , C12N15/63 , C07K19/00 , C12N15/62
CPC分类号： C12Q1/37 , C07K2319/00 , C12N15/85
摘要： This invention relates to a probe reagent comprising, in order from the N-terminus to the C-terminus, the amino acid sequences of a fluorescent protein I, a peptide capable of terminating protein degradation (i.e., a degradation-terminating peptide), a spacer peptide, a fluorescent protein II, and a protein to be degraded, wherein the protein to be degraded is a protein degraded by the ubiquitin-proteasome system, and the probe reagent is degraded from the C-terminus, but that the degradation of the probe reagent is terminated at the degradation-terminating peptide, a nucleic acid encoding the probe reagent, and use of the probe reagent or the nucleic acid.
摘要翻译：本发明涉及探针试剂，其从N末端到C末端的顺序包括荧光蛋白I的氨基酸序列，能够终止蛋白质降解的肽（即降解终止肽），间隔肽，荧光蛋白II和待降解的蛋白质，其中待降解的蛋白质是由泛素 - 蛋白酶体系统降解的蛋白质，并且探针试剂从C末端降解，但是探针试剂终止于降解终止肽，编码探针试剂的核酸和探针试剂或核酸的使用。

48. 发明授权

US08304240B2 Microinjection method and device 有权
标题翻译：显微注射法和装置
公开(公告)号：US08304240B2
公开(公告)日：2012-11-06
申请号：US12464038
申请日：2009-05-11
申请人： Atsushi Miyawaki , Hiroyuki Imabayashi , Sachiko Karaki
发明人： Atsushi Miyawaki , Hiroyuki Imabayashi , Sachiko Karaki
IPC分类号： C12N15/00
CPC分类号： B82Y30/00 , C12M35/00
摘要： An object of the present invention is to provide a method for introducing a physiologically active substance such as a gene into a cell, which introduces a physiologically active substance such as any given gene into any given cell in a view under a microscope, while significantly reducing invasiveness to the cell, and a device used for the above method. The present invention provides a method for introducing a physiologically active substance into a cell, which comprises: allowing a physiologically active substance to attach around a needle having a diameter of 500 nm or less, provided that it is able to be inserted into a cell; and inserting the above-described needle into the cell; and a microinjection device for carrying out the aforementioned method.
摘要翻译：本发明的目的是提供一种将生物活性物质如基因导入细胞的方法，该细胞在显微镜下以任何给定的基因引入任何给定基因的生理活性物质，同时显着降低对细胞的侵袭性，以及用于上述方法的装置。本发明提供了一种将生理活性物质引入细胞的方法，其包括：使生理活性物质附着在直径为500nm以下的针上，只要其能够插入细胞中即可。并将上述针插入细胞中; 以及用于实施上述方法的显微注射装置。

49. 发明授权

US08017746B2 Fluorescent protein 有权
标题翻译：荧光蛋白
公开(公告)号：US08017746B2
公开(公告)日：2011-09-13
申请号：US10581551
申请日：2004-12-03
申请人： Atsushi Miyawaki , Takako Kogure , Hiroshi Hama , Masataka Kinjo , Kenta Saito , Satoshi Karasawa , Toshio Araki
发明人： Atsushi Miyawaki , Takako Kogure , Hiroshi Hama , Masataka Kinjo , Kenta Saito , Satoshi Karasawa , Toshio Araki
IPC分类号： C07H21/02 , C12Q1/02 , C12N1/20
CPC分类号： C07K14/37 , C07K14/43504 , G01N33/533
摘要： An object of the present invention is to provide a red or orange fluorescent protein, which is characterized in that the difference (stokes shift) between an excitation peak value (wavelength of maximum absorption) and a fluorescence peak value (wavelength of maximum fluorescence) is greatened, so that the maximum fluorescence can be obtained by the maximum excitation. The present invention provides a novel fluorescent protein monomerized by introducing a mutation into a florescent protein derived from Fungia sp., and a novel chromoprotein and fluorescent protein derived from Montipora. sp.
摘要翻译：本发明的目的是提供一种红色或橙色荧光蛋白，其特征在于，激发峰值（最大吸收波长）与荧光峰值（最大荧光的波长）之间的差异（斯托克斯偏移）为从而可以通过最大激发获得最大荧光。本发明提供了一种新颖的荧光蛋白，其通过将突变引入到来自真菌属的荧光蛋白中而单体化，以及衍生自Montipora的新型生色蛋白和荧光蛋白。 sp。

50. 发明申请

US20100151514A1 NOVEL MUCIN-TYPE GLYCOPROTEIN AND USE THEREOF 有权
标题翻译：新型葡萄糖型糖蛋白及其用途
公开(公告)号：US20100151514A1
公开(公告)日：2010-06-17
申请号：US12063498
申请日：2006-08-11
申请人： Kiminori Ushida , Akiko Masuda , Naoshi Dohmae , Hidemitsu Furukawa , Atsushi Miyawaki
发明人： Kiminori Ushida , Akiko Masuda , Naoshi Dohmae , Hidemitsu Furukawa , Atsushi Miyawaki
IPC分类号： C12P21/02 , C07K9/00 , C07K14/00 , C07K7/08 , C07K7/06
CPC分类号： C07K14/4727 , A23J1/04 , A23J3/04 , A23L33/17 , A61K38/00
摘要： Provided is a novel mucin-type glycoprotein and a method for producing the same. Specifically, a mucin-type glycoprotein having a repeat structure including 3 to 2000 repeating units each having an amino acid sequence represented by the formula I: Val-Xaa-Glu-Thr-Thr-Ala-Ala-Pro [wherein Xaa represents Val or Ile] (SEQ ID NO: 1), wherein one or more amino acid residues in the structure are bound to a sugar chain of one or more monosaccharides. Also provided is a composition containing the novel mucin-type glycoprotein. Further provided is a molecular weight marker containing the novel mucin-type glycoprotein.
摘要翻译：本发明提供一种新型粘蛋白型糖蛋白及其制造方法。具体地说，具有重复结构的粘蛋白型糖蛋白，其重复结构包括3〜2000个重复单元，每个重复单元各自具有由式I表示的氨基酸序列：Val-Xaa-Glu-Thr-Thr-Ala-Ala-Pro [其中Xaa表示Val或 Ile]（SEQ ID NO：1），其中结构中的一个或多个氨基酸残基与一个或多个单糖的糖链结合。还提供含有新型粘蛋白型糖蛋白的组合物。还提供含有新型粘蛋白型糖蛋白的分子量标记物。

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