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41. 发明授权

US5188934A 4,7-dichlorofluorescein dyes as molecular probes 失效
标题翻译： 4,7-DICHLOROFUORESCEIN DYES AS MOLECULAR PROBES
公开(公告)号：US5188934A
公开(公告)日：1993-02-23
申请号：US436455
申请日：1989-11-14
申请人： Steven M. Menchen , Linda G. Lee , Charles R. Connell , N. Davis Hershey , Vergine Chakerian , Sam Woo , Steven Fung
发明人： Steven M. Menchen , Linda G. Lee , Charles R. Connell , N. Davis Hershey , Vergine Chakerian , Sam Woo , Steven Fung
IPC分类号： C07D311/82 , C07H21/00 , C09B11/08 , C12Q1/68 , G01N21/64 , G01N21/78 , G01N27/447 , G01N33/58
CPC分类号： C12Q1/6869 , C07D311/82 , C07H21/00 , C09B11/08 , C12Q1/6816 , Y10S435/968 , Y10S436/80
摘要： Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 1',2',7',8'-dibenzo-4,7-dichlorofluoresceins. Methods of using the dyes in automated DNA sequencing are described.
摘要翻译：提供长波长，窄发射带宽荧光素染料用于检测空间重叠的目标物质。染料包括4,7-二氯荧光素，特别是1'，2'，7'，8'-二苯并-4,7-二氯荧光素。描述了在自动DNA测序中使用染料的方法。

42. 发明授权

US08993632B2 Inhibitors of the 11-beta-hydroxysteroid dehydrogenase type 1 enzyme 有权
标题翻译： 11-β-羟基类固醇脱氢酶1型酶的抑制剂
公开(公告)号：US08993632B2
公开(公告)日：2015-03-31
申请号：US13469021
申请日：2012-05-10
申请人： R. Scott Bitner , Kaitlin E. Browman , Michael E. Brune , Steven Fung , Peer B. Jacobson , Lynne E. Rueter , Marina I. Strakhova , Jiahong Wang , Jeffrey J. Rohde , Qi Shuai , James T. Link , Jyoti R. Patel , Jurgen Dinges , Bryan K. Sorensen , Hong Yong , Vince S. Yeh , Ravi Kurukulasuriya
发明人： R. Scott Bitner , Kaitlin E. Browman , Michael E. Brune , Steven Fung , Peer B. Jacobson , Lynne E. Rueter , Marina I. Strakhova , Jiahong Wang , Jeffrey J. Rohde , Qi Shuai , James T. Link , Jyoti R. Patel , Jurgen Dinges , Bryan K. Sorensen , Hong Yong , Vince S. Yeh , Ravi Kurukulasuriya
IPC分类号： A61K31/16 , C07C233/11 , A61K31/165 , A61K31/195 , A61K31/44 , A61K31/45 , A61K31/5377 , C07C233/52 , C07C233/58 , C07C233/63 , C07C237/24 , C07C311/46 , C07D207/277 , C07D211/78 , C07D213/56 , C07D231/12 , C07D261/08 , C07D333/24
CPC分类号： A61K31/498 , A61K31/165 , A61K31/195 , A61K31/196 , A61K31/381 , A61K31/4015 , A61K31/415 , A61K31/44 , A61K31/4409 , A61K31/45 , A61K31/451 , A61K31/5377 , C07C233/11 , C07C233/52 , C07C233/58 , C07C233/63 , C07C237/24 , C07C311/46 , C07C2601/02 , C07C2601/04 , C07C2601/08 , C07C2601/14 , C07C2603/74 , C07D207/277 , C07D211/78 , C07D213/56 , C07D231/12 , C07D261/08 , C07D333/24
摘要： The present invention relates to inhibitors of the 11-beta-hydroxysteroid dehydrogenase Type 1 enzyme and their use in treatment of non-insulin dependent type 2 diabetes, insulin resistance, obesity, lipid disorders, metabolic syndrome, central nervous system disorders, and diseases and conditions that are related to excessive glucocorticoids.
摘要翻译：本发明涉及11-β-羟基类固醇脱氢酶1型酶的抑制剂及其在治疗非胰岛素依赖性2型糖尿病，胰岛素抵抗，肥胖症，脂质障碍，代谢综合征，中枢神经系统疾病和疾病中的用途，以及与过量糖皮质激素有关的病症。

43. 发明授权

US08198331B2 Inhibitors of the 11-beta-hydroxysteroid dehydrogenase type 1 enzyme 有权
标题翻译： 11-β-羟基类固醇脱氢酶1型酶的抑制剂
公开(公告)号：US08198331B2
公开(公告)日：2012-06-12
申请号：US12390286
申请日：2009-02-20
申请人： R. Scott Bitner , Kaitlin E. Browman , Michael E. Brune , Steven Fung , Peer B. Jacobson , Lynne E. Rueter , Marina I. Strakhova , Jiahong Wang , Jeffrey J. Rohde , Qi Shuai , James T. Link , Jyoti R. Patel , Jurgen Dinges , Bryan K. Sorensen , Hong Yong , Vince S. Yeh , Ravi Kurukulasuriya
发明人： R. Scott Bitner , Kaitlin E. Browman , Michael E. Brune , Steven Fung , Peer B. Jacobson , Lynne E. Rueter , Marina I. Strakhova , Jiahong Wang , Jeffrey J. Rohde , Qi Shuai , James T. Link , Jyoti R. Patel , Jurgen Dinges , Bryan K. Sorensen , Hong Yong , Vince S. Yeh , Ravi Kurukulasuriya
IPC分类号： A61K31/16
CPC分类号： A61K31/498 , A61K31/165 , A61K31/195 , A61K31/196 , A61K31/381 , A61K31/4015 , A61K31/415 , A61K31/44 , A61K31/4409 , A61K31/45 , A61K31/451 , A61K31/5377 , C07C233/11 , C07C233/52 , C07C233/58 , C07C233/63 , C07C237/24 , C07C311/46 , C07C2601/02 , C07C2601/04 , C07C2601/08 , C07C2601/14 , C07C2603/74 , C07D207/277 , C07D211/78 , C07D213/56 , C07D231/12 , C07D261/08 , C07D333/24
摘要： The present invention relates to inhibitors of the 11-beta-hydroxysteroid dehydrogenase Type 1 enzyme and their use in treatment of non-insulin dependent type 2 diabetes, insulin resistance, obesity, lipid disorders, metabolic syndrome, central nervous system disorders, and diseases and conditions that are related to excessive glucocorticoids.
摘要翻译：本发明涉及11-β-羟基类固醇脱氢酶1型酶的抑制剂及其在治疗非胰岛素依赖性2型糖尿病，胰岛素抵抗，肥胖症，脂质障碍，代谢综合征，中枢神经系统疾病和疾病中的用途，以及与过量糖皮质激素有关的病症。

44. 发明申请

US20090192198A1 INHIBITORS OF THE 11-BETA-HYDROXYSTEROID DEHYDROGENASE TYPE 1 ENZYME 审中-公开
标题翻译： 11-羟基脱氢酶脱氢酶1型酶的抑制剂
公开(公告)号：US20090192198A1
公开(公告)日：2009-07-30
申请号：US12390318
申请日：2009-02-20
申请人： R. Scott Bitner , Kaitlin E. Browman , Michael E. Brune , Steven Fung , Peer B. Jacobson , Lynne E. Rueter , Marina I. Strakhova , Jiahong Wang , Jyoti R. Patel , Qi Shuai , James T. Link , Jeffrey J. Rohde , Jurgen Dinges , Bryan K. Sorensen , Martin Winn , Hong Yong , Vince S. Yeh
发明人： R. Scott Bitner , Kaitlin E. Browman , Michael E. Brune , Steven Fung , Peer B. Jacobson , Lynne E. Rueter , Marina I. Strakhova , Jiahong Wang , Jyoti R. Patel , Qi Shuai , James T. Link , Jeffrey J. Rohde , Jurgen Dinges , Bryan K. Sorensen , Martin Winn , Hong Yong , Vince S. Yeh
IPC分类号： A61K31/4439 , A61K31/19 , A61P35/00 , A61K31/165
CPC分类号： C07C235/14 , A61K31/165 , A61K31/19 , A61K31/4439 , C07C235/22 , C07C237/24 , C07C271/28 , C07C311/46 , C07C2601/14 , C07C2601/18 , C07C2603/74 , C07D207/48 , C07D257/04 , C07D261/08 , C07D295/088 , C07D401/04
摘要： The present invention relates to inhibitors of the 11-beta-hydroxysteroid dehydrogenase Type 1 enzyme and their use in treatment of non-insulin dependent type 2 diabetes, insulin resistance, obesity, lipid disorders, metabolic syndrome, central nervous system disorders, and diseases and conditions that are related to excessive glucocorticoids.
摘要翻译：本发明涉及11-β-羟基类固醇脱氢酶1型酶的抑制剂及其在治疗非胰岛素依赖性2型糖尿病，胰岛素抵抗，肥胖症，脂质障碍，代谢综合征，中枢神经系统疾病和疾病中的用途，以及与过量糖皮质激素有关的病症。

45. 发明申请

US20050112609A1 Probe composition and method 失效
标题翻译：探头组成和方法
公开(公告)号：US20050112609A1
公开(公告)日：2005-05-26
申请号：US10825624
申请日：2004-04-14
申请人： Paul Grossman , Steven Fung , Steven Menchen , Sam Woo , Emily Winn-Deen
发明人： Paul Grossman , Steven Fung , Steven Menchen , Sam Woo , Emily Winn-Deen
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q1/6825 , C12Q1/6827 , C12Q1/686 , C12Q1/6862 , C12Q2600/156 , G01N27/447 , Y10T436/143333 , C12Q2525/197 , C12Q2525/203 , C12Q2537/143 , C12Q2565/131 , C12Q2565/125 , C12Q2565/107 , C12Q2521/501 , C12Q2563/155 , C12Q2563/119 , C12Q2549/119
摘要： Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieving medium.
摘要翻译：用于检测靶多核苷酸中的一个或多个选择的多核苷酸区域的方法和组合物。在该方法中，将序列特异性探针的混合物与靶多核苷酸在杂交条件下反应，并对杂交的探针进行处理以选择性地修饰以碱基特异性方式结合靶多核苷酸的那些探针。所得到的标记探针包括赋予每个不同序列探针的聚合物链，电荷/平移摩擦阻力的独特比例和可检测标记。标记的探针通过在非筛选基质中的电泳分级分离，并且根据在非筛选培养基中标记的探针的观察到的电泳迁移速率来检测靶多核苷酸中一个或多个选定序列的存在。

46. 发明授权

US5625052A Method for preparing 3'-nitrogen containing polynucleotides 失效
标题翻译：制备含3'-氮的多核苷酸的方法
公开(公告)号：US5625052A
公开(公告)日：1997-04-29
申请号：US576206
申请日：1995-12-21
申请人： Sam L. Woo , Steven Fung
发明人： Sam L. Woo , Steven Fung
IPC分类号： C12N15/09 , C07C217/20 , C07C271/10 , C07C271/16 , C07C271/66 , C07C317/18 , C07C333/04 , C07H21/00 , C07H21/04 , C07H1/00
CPC分类号： C07H21/00 , Y02P20/55
摘要： A method for synthesizing a polynucleotide having a nitrogen atom at the 3'-end is disclosed. The methods include the following steps. A solid support reagent is provided having the structure shown below: ##STR1## where T is an acid-cleavable hydroxyl protecting group, e.g., 4,4'-dimethoxytritil; Q is a linker connecting the nitrogen and oxygen, e.g., n-hexyl; R.sub.1 is a nitrogen substituent, e.g., hydrogen; R.sub.2 through R.sub.4 are separately hydrogen or lower alkyl; Y is an atom which is electronegative with respect to carbon, e.g., oxygen or sulfur; X.sub.1 is an atom which is electronegative with respect to carbon, e.g., sulfone; Z is a bond or spacer arm, e.g., ethylsuccinate; and W is a derivatized solid synthesis support capable of linking to Z, e.g., an amino-dirivitized controlled pore glass. The solid support is treated with acid to remove the acid-cleavable hydroxyl protecting group. A protected nucleoside monomer and a weak acid is added forming a linkage, and the unreacted sites on the solid support are capped. An oxidizing reagent is added and the above steps are repeated until polynucleotide chain elongation is complete. The polynucleotide is cleaved from the solid support and then deprotected.
摘要翻译：公开了一种在3'端合成具有氮原子的多核苷酸的方法。方法包括以下步骤。提供具有如下结构的固体支持剂：其中T是酸可裂解的羟基保护基团，例如4,4'-二甲氧基三苯乙酮; Q是连接氮和氧的接头，例如正己基; R1是氮取代基，例如氢; R2至R4分别为氢或低级烷基; Y是相对于碳例如氧或硫具有电负性的原子; X1是相对于碳电负性的原子，例如砜; Z是键或间隔臂，例如乙基琥珀酸盐; 并且W是能够与Z连接的衍生的固体合成载体，例如氨基 - 二硝基化的受控孔玻璃。用酸处理固体支持物以除去酸可裂解的羟基保护基团。加入保护的核苷单体和弱酸形成连接，并将固体载体上的未反应部位加封。加入氧化试剂，并重复上述步骤，直到多核苷酸链延伸完成。多核苷酸从固体支持物上切割，然后去保护。

47. 发明授权

US5593826A Enzymatic ligation of 3'amino-substituted oligonucleotides 失效
标题翻译： 3'-氨基取代寡核苷酸的酶连接
公开(公告)号：US5593826A
公开(公告)日：1997-01-14
申请号：US48975
申请日：1993-04-16
申请人： Steven Fung , Sergei M. Gryaznov
发明人： Steven Fung , Sergei M. Gryaznov
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6862
摘要： Method and kits are provided for detecting one or more target nucleic acids. A first oligonucleotide having a 3' amino group and a second oligonucleotide having a 5' phosphate group are annealed to a contiguous complementary region of a target nucleic acid. Whenever the 3' terminal nucleotides of the first oligonucleotide and the 5' nucleotides of the second oligonucleotide are complementary to the opposing nucleotides on the target nucleic acid, a nucleic acid ligase ligates the first and second oligonucleotides via the formation of a phosphoramidate linkage. The presence of the target nucleic acid is determined by detection of the ligated first and second oligonucleotides.
摘要翻译：提供了用于检测一种或多种靶核酸的方法和试剂盒。具有3'氨基的第一寡核苷酸和具有5'磷酸基团的第二寡核苷酸与目标核酸的连续互补区退火。每当第一寡核苷酸的3'末端核苷酸和第二寡核苷酸的5'核苷酸与靶核酸上的相对核苷酸互补时，核酸连接酶通过形成氨基磷酸酯连接物连接第一和第二寡核苷酸。通过检测连接的第一和第二寡核苷酸来确定靶核酸的存在。

48. 发明授权

US5580732A Method of DNA sequencing employing a mixed DNA-polymer chain probe 失效
标题翻译：使用混合DNA-聚合物链探针进行DNA测序的方法
公开(公告)号：US5580732A
公开(公告)日：1996-12-03
申请号：US296880
申请日：1994-08-26
申请人： Paul D. Grossman , Steven Fung , Steven M. Menchen , Sam L. Woo , Emily S. Winn-Deen
发明人： Paul D. Grossman , Steven Fung , Steven M. Menchen , Sam L. Woo , Emily S. Winn-Deen
IPC分类号： C12N15/09 , B01D57/02 , C12Q1/68 , G01N27/447 , G01N30/88 , G01N33/566 , C12P19/34
CPC分类号： C12Q1/6869 , C12Q1/6816 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q1/6862 , C12Q1/6818 , Y10T436/143333
摘要： Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieiving medium.
摘要翻译：用于检测靶多核苷酸中的一个或多个选择的多核苷酸区域的方法和组合物。在该方法中，将序列特异性探针的混合物与靶多核苷酸在杂交条件下反应，并对杂交的探针进行处理以选择性地修饰以碱基特异性方式结合靶多核苷酸的那些探针。所得到的标记探针包括赋予每个不同序列探针的聚合物链，电荷/平移摩擦阻力的独特比例和可检测标记。标记的探针通过在非筛选基质中的电泳进行分级，并且根据在非筛选培养基中标记的探针的观察到的电泳迁移速率来检测靶多核苷酸中一个或多个选定序列的存在。

49. 发明授权

US5470705A Probe composition containing a binding domain and polymer chain and methods of use 失效
标题翻译：含有结合域和聚合物链的探针组合物及其使用方法
公开(公告)号：US5470705A
公开(公告)日：1995-11-28
申请号：US866018
申请日：1992-04-07
申请人： Paul D. Grossman , Steven Fung , Steven M. Menchen , Sam L. Woo , Emily S. Winn-Deen
发明人： Paul D. Grossman , Steven Fung , Steven M. Menchen , Sam L. Woo , Emily S. Winn-Deen
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q1/6825 , C12Q1/6827 , C12Q1/686 , C12Q1/6862 , C12Q2600/156 , G01N27/447 , Y10T436/143333
摘要： Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieving medium.
摘要翻译：用于检测靶多核苷酸中的一个或多个选择的多核苷酸区域的方法和组合物。在该方法中，将序列特异性探针的混合物与靶多核苷酸在杂交条件下反应，并对杂交的探针进行处理以选择性地修饰以碱基特异性方式结合靶多核苷酸的那些探针。所得到的标记探针包括赋予每个不同序列探针的聚合物链，电荷/平移摩擦阻力的独特比例和可检测标记。标记的探针通过在非筛选基质中的电泳分级分离，并且根据在非筛选培养基中标记的探针的观察到的电泳迁移速率来检测靶多核苷酸中一个或多个选定序列的存在。

50. 发明授权

US5258538A 2,3-disubstituted-1,3,2-oxazaphosphacycloalkanes as nucleic acid linking agents 失效
标题翻译： 2,3-二取代-1,3,2-氧杂氮杂环烷烃作为核酸连接剂
公开(公告)号：US5258538A
公开(公告)日：1993-11-02
申请号：US734575
申请日：1991-10-29
申请人： Steven Fung , Sam L. Woo , Lloyd M. Smith
发明人： Steven Fung , Sam L. Woo , Lloyd M. Smith
IPC分类号： A61K47/48 , C07F9/24 , C07F9/6584 , C07H21/00 , C07F9/00
CPC分类号： C07H21/00 , A61K47/48023 , A61K47/48061 , A61K47/48084 , C07F9/2408 , C07F9/65842
摘要： The compounds of the invention include novel linking agents comprising 2-substituted-3-protected-1,3,2-oxazaphosphacycloalkanes and their phosphoramidite precursors. The compounds of the invention further include conjugates of the above linking agents with oligonucleotides and polymer supports. The compounds of the present invention are useful for linking organic moieties, such as fluorescent or chromogenic dyes, to polymer supports and oligonucleotides, particularly single- and double-stranded DNA and RNA fragments.
摘要翻译：本发明的化合物包括包含2-取代-3-保护的1,3,2-氧杂氮杂环烷烃及其亚磷酰胺前体的新型交联剂。本发明的化合物还包括上述连接剂与寡核苷酸和聚合物载体的缀合物。本发明的化合物可用于将有机部分如荧光或显色染料与聚合物载体和寡核苷酸，特别是单链和双链DNA和RNA片段连接。

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