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41. 发明授权

US07732426B2 Isocyclomaltooligosaccharide (S) , isocyclomaltooligosaccharide-forming enzyme, their preparation and uses 有权
标题翻译：异环寡糖（S），异环寡糖寡糖形成酶，其制备和用途
公开(公告)号：US07732426B2
公开(公告)日：2010-06-08
申请号：US11663919
申请日：2005-09-26
申请人： Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： A61K31/715 , A61K31/702 , C07H1/00
CPC分类号： C07H3/06 , A23L29/35 , A23L33/25 , C12N9/2402 , C12N9/2405 , C12P19/04
摘要： The present invention have objects to provide an option of non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents and to provide a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof. The present invention solves the above objects by providing an isocyclomaltooligosaccharide(s) having a structure represented by General Formula 1, a novel isocyclomaltooligosaccharide-forming enzyme, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the isocyclomaltooligosaccharide(s) or a saccharide composition comprising the same, and uses thereof. Cyclo{→6)-[α-D-Glcp-(1→4)]n-α-D-Glcp-(1→} General Formula 1 (In General Formula 1, “n” means a number of 4 or 5).
摘要翻译：本发明的目的是提供一种由葡萄糖组成的新型非还原性糖组成的非还原性糖类，提供形成非还原性糖类的新型酶，其制造方法及其制造方法，编码酶的DNA，重组DNA和包含DNA的转化体，包含非还原性糖的组合物及其用途。本发明通过提供具有由通式1表示的结构的异环型低聚半乳糖，新型的异环型低聚葡萄糖形成酶，其制备方法和方法，编码该酶的DNA，重组DNA和转化体来解决上述目的包含DNA，包含异环寡脱醇糖的组合物或包含该组合物的糖组合物及其用途。环状{→6） - [α-D-Glcp-（1→4）] n-α-D-Glcp-（1→}通式1（在通式1中，“n”表示4或5 ）。

42. 发明申请

US20080214499A1 Lipid-Regulating Agent and Use Thereof 有权
标题翻译：脂质调节剂及其用途
公开(公告)号：US20080214499A1
公开(公告)日：2008-09-04
申请号：US12033654
申请日：2008-02-19
申请人： Kazuyuki Oku , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Kazuyuki Oku , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： A61K31/702 , A61P3/06
CPC分类号： A23D9/007 , A23K20/163 , A23L33/10 , A61K31/702 , C07H3/06
摘要： The object of the present invention is to provide a lipid-regulating agent or a composition for regulating the amount of lipids comprising the agent. The present invention solves the above object by providing a lipid-regulating agent comprising a cyclic tetrasaccharide and/or its saccharide-derivative(s) and a composition for regulating the amount of lipids comprising the lipid-regulating agent.
摘要翻译：本发明的目的是提供一种脂质调节剂或用于调节包含该药剂的脂质的量的组合物。本发明通过提供包含环状四糖和/或其糖衍生物的脂质调节剂和用于调节包含脂质调节剂的脂质的量的组合物来解决上述目的。

43. 发明申请

US20080032350A1 Alpha-isomaltosylglucosaccharide-forming enzyme, process and uses of the same 有权
标题翻译： α-异麦芽糖基葡萄糖形成酶，其工艺和用途相同
公开(公告)号：US20080032350A1
公开(公告)日：2008-02-07
申请号：US11797969
申请日：2007-05-09
申请人： Michio Kubota , Keiji Tsusaki , Takanobu Higashiyama , Shigeharu Fukuda , Toshio Miyake
发明人： Michio Kubota , Keiji Tsusaki , Takanobu Higashiyama , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12P19/04
CPC分类号： A23G1/40 , A21D2/181 , A23C9/005 , A23C9/1307 , A23C9/1544 , A23C9/156 , A23G3/42 , A23G4/10 , A23G9/34 , A23L2/60 , A23L15/00 , A23L15/20 , A23L27/30 , A23L33/10 , A23L33/21 , A61K8/60 , A61K8/66 , A61K9/0014 , A61K47/26 , A61Q11/00 , A61Q19/00 , A61Q19/02 , A61Q19/10 , C07H3/04 , C07H3/06 , C12N9/1051 , C12N9/2425 , C12N9/2428 , C12P19/04 , C12Y302/01001 , C12Y302/01002 , C12Y302/01003 , C12Y302/0102
摘要： The object of the present invention is to provide an α-isomaltosylglucosaccharide-forming enzyme, process of the same, cyclotetrasaccharide, and saccharide composition comprising the saccharide which are obtainable by using the enzyme; and is solved by establishing an α-isomaltosylglucosaccharide-forming enzyme which forms a saccharide, having a glucose polymerization degree of at least three and having both the α-1,6 glucosidic linkage as a linkage at the non-reducing end and the α-1,4 glucosidic linkage other than the linkage at the non-reducing end, by catalyzing the α-glucosyl-transfer from a saccharide having a glucose polymerization degree of at least two and having the α-1,4 glucosidic linkage as a linkage at the non-reducing end without substantially increasing the reducing power; α-isomaltosyl-transferring method using the enzyme; method for forming α-isomaltosylglucosaccharide; process for producing a cyclotetrasaccharide having the structure of cyclo{66)-α-D-glucopyranosyl-(163)-α-D-glucopyranosyl-(166)-α-D-glucopyranosyl-(163)-α-D-glucopyranosyl-(16} using both the α-isomaltosylglucosaccharide-forming enzyme and the α-isomaltosyl-transferring enzyme; and the uses of the saccharides obtainable therewith.
摘要翻译：本发明的目的是提供一种α-异麦芽糖基葡萄糖形成酶，其工艺，环四糖和包含可通过使用酶获得的糖的糖组合物; 并且通过建立形成葡糖聚合度至少为3并且在非还原末端具有α-1,6糖苷键作为连接的α糖异构体糖结合酶和α - 通过催化从葡萄糖聚合度至少为2并具有α-1,4糖苷键作为连接的糖的α-葡糖基转移而在非还原性末端的连接以外的1,4-葡糖苷键所述非还原端基本上不增加所述还原能力; 使用该酶的α-异麦芽糖基转移法; 形成α-异麦芽糖基糖的方法; 制备具有环{66）-alpha-D-吡喃葡萄糖基 - （163）-al-D-glucopyranosyl-（166）-al-D-glucopyranosyl-（163）-al-D-glucopyranosyl-（163）-al-D-glucopyranosyl- （16）同时使用α-异麦芽糖基葡萄糖形成酶和α-异麦芽糖基转移酶;以及可用其获得的糖的用途。

44. 发明授权

US07309697B2 Cyclic maltosylmatose 有权
标题翻译：循环麦芽糖
公开(公告)号：US07309697B2
公开(公告)日：2007-12-18
申请号：US10569959
申请日：2004-08-26
申请人： Kazuhisa Mukai , Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Kazuhisa Mukai , Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： A61K31/715 , C07H1/00
CPC分类号： A61K9/2018 , A21D2/181 , A23L2/52 , A23L2/60 , A23L13/428 , A23L27/33 , A23L33/10 , A61K8/60 , A61Q11/00 , C07H3/06 , C12N9/1048 , C12P19/18
摘要： An object of the present invention is to provide an option of non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents and to provide a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof. The present invention solves the above object by providing a novel cyclic saccharide having a structure of cyclo{→6)-α-D-glucopyranosyl-(1→4)-α-D-glucopyranosyl-(1→6)-α-D-glucopyranosyl-(1→4)-α-D-glucopyranosyl-(1→6}, cyclic maltosylmaltose, novel cyclic maltosylmaltose-forming enzyme, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the cyclic maltosylmaltose or a saccharide composition comprising the same, and uses thereof.
摘要翻译：本发明的目的是通过提供一种由葡萄糖组成的新型非还原性糖组成并提供形成非还原性糖的新型酶，提供一种非还原性糖的方法及其制备方法，编码该酶的DNA，重组DNA和包含该DNA的转化体，包含非还原性糖的组合物及其用途。本发明通过提供具有环{ - > 6）-α-D-吡喃葡萄糖基 - （1-> 4）-α-D-吡喃葡萄糖基 - （1-> 6） - 结构的新型环状糖来解决上述目的。 α-D-吡喃葡萄糖基 - （1-> 4）-α-D-吡喃葡萄糖基 - （1-> 6），环状麦芽糖基麦芽糖，新型环状麦芽糖基麦芽糖酶形成酶，其制备方法和方法，编码酶，重组DNA和包含该DNA的转化体，包含环状麦芽糖基麦芽糖的组合物或包含其的糖组合物及其用途。

45. 发明申请

US20060177553A1 Method of powdering nonsugar component and powdering base 审中-公开
标题翻译：非糖份成分粉化基粉的方法
公开(公告)号：US20060177553A1
公开(公告)日：2006-08-10
申请号：US10550486
申请日：2004-03-23
申请人： Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： A23P1/00
CPC分类号： A23P10/40 , A23L5/00 , A23L7/00 , A23L9/00 , A23L21/00 , A23L27/38 , A23L29/30 , A23V2002/00 , A23V2250/636
摘要： The objects of the present invention are to provide a method for powderizing a non-saccharide ingredient(s), a powdery composition prepared by the method, and a base for powderizing a non-saccharide ingredient(s). The present invention solves the above objects by providing a method for powderizing a non-saccharide ingredient(s) which comprises the steps of allowing a saccharide-derivative(s) of α,α-trehalose to incorporate into a non-saccharide ingredient(s) and powderizing the resulting mixture; a powdery composition prepared by the method; and a base for powderizing non-saccharide ingredient(s) comprising the saccharide-derivative(s) as an effective ingredient(s).
摘要翻译：本发明的目的是提供一种用于粉化非糖成分的方法，通过该方法制备的粉末组合物和用于粉化非糖成分的碱。本发明通过提供一种粉末化非糖成分的方法来解决上述目的，该方法包括以下步骤：将α，α-海藻糖的糖衍生物掺入非糖成分中）并粉碎所得混合物; 通过该方法制备的粉状组合物; 和用于粉碎包含作为有效成分的糖衍生物的非糖成分的基料。

46. 发明申请

US20050013914A1 Method of sustaining aroma and use thereof 审中-公开
标题翻译：维持香气及其使用的方法
公开(公告)号：US20050013914A1
公开(公告)日：2005-01-20
申请号：US10496382
申请日：2002-11-21
申请人： Kazuyuki Oku , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Kazuyuki Oku , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： A23L3/349 , A23L3/3508 , A23L27/00 , A61K8/60 , A61Q5/00 , A61Q5/02 , A61Q5/06 , A61Q11/00 , A61Q13/00 , A61Q17/00 , A61Q19/00 , A61Q19/10 , C11B9/00 , A23B4/00
CPC分类号： A61Q19/00 , A23L27/70 , A23V2002/00 , A61K8/60 , A61K2800/56 , A61Q5/00 , A61Q5/02 , A61Q5/06 , A61Q11/00 , A61Q13/00 , A61Q17/005 , A61Q19/10 , A23V2200/15 , A23V2250/60
摘要： The first object of the present invention is to provide a method for preparing an aroma-retaining material, which enables to increase the adsorbed aroma amount, retain the aroma for a long period of time, and impart stability. The second object of the present invention is to provide an aroma-retaining material, which can retain aroma for a long period of time and has satisfactory stability. The third object of the present invention is to provide a composition comprising the aroma-retaining material. The forth object of the present invention is to provide an aroma-retaining agent, which enables to stabilize an aromatic substance. The fifth object of the present invention is to provide a retained-releasing bactericide comprising the aroma-retaining material. The present invention solves the above objects by providing a method for retaining an aroma in such a manner of mixing an aromatic substance and a cyclic tetrasaccharide or the mixture of cyclic tetrasaccharide and its saccharide derivative(s), an aroma-retaining material obtainable by the method, a composition comprising the aroma-retaining material, an aroma-retaining agent comprising a cyclic tetrasaccharide or a mixture of a cyclic tetrasaccharide and its saccharide derivative(s) as effective ingredients, and a bactericide using the aroma-retaining material having a sustained-release property.
摘要翻译：本发明的第一个目的是提供一种制备香料保持材料的方法，其能够增加吸附的香味量，长时间保持香气并赋予其稳定性。本发明的第二个目的是提供一种能够长时间保持香气并且具有令人满意的稳定性的香气保持材料。本发明的第三个目的是提供一种包含芳香保持材料的组合物。本发明的第四个目的是提供一种使芳族物质稳定的香味保持剂。本发明的第五个目的是提供一种包含香料保留材料的保留释放杀菌剂。本发明通过提供一种芳香物质与环状四糖或环状四糖及其糖衍生物的混合物的混合方式来保持香气的方法，其中芳香保留物可通过方法，包含芳香保持材料的组合物，包含环状四糖或环状四糖及其糖衍生物作为有效成分的混合物的香料保留剂，以及使用具有持续性的香料保留材料的杀菌剂释放财产。

47. 发明授权

US06808651B1 Thermoplastic shaped-body of trehalose, process and uses thereof 有权
标题翻译：海藻糖的热塑性体，其制法及用途
公开(公告)号：US06808651B1
公开(公告)日：2004-10-26
申请号：US09694677
申请日：2000-10-24
申请人： Naohiko Katagiri , Yasuo Takeuchi , Michio Kubota , Toshio Miyake
发明人： Naohiko Katagiri , Yasuo Takeuchi , Michio Kubota , Toshio Miyake
IPC分类号： A23L33562
CPC分类号： C08B37/00 , C08L5/00 , Y10T428/2991 , Y10T428/8305
摘要： A thermoplastic shaped-body of trehalose obtainable by concentrating a trehalose solution by heating either to lower the moister content of the solution to a level of not higher than about 8.3 w/w % in the presence of a substance capable of lowering the adhesion of a trehalose solution, or to lower the moisture content of the trehalose solution to a level of not higher than about 2.4 w/w %.
摘要翻译：一种海绵糖的热塑性成形体，可通过加热将浓缩海藻糖溶液，将溶液的含水量降低到不高于约8.3w / w％的水平，在能够降低粘度的物质的存在下，海藻糖溶液，或将海藻糖溶液的水分含量降低到不高于约2.4w / w％的水平。

48. 发明授权

US6150153A Thermostable trehalose-releasing enzyme 失效
标题翻译：热稳定性海藻糖释放酶
公开(公告)号：US6150153A
公开(公告)日：2000-11-21
申请号：US888158
申请日：1997-07-03
申请人： Shouji Ikegami , Michio Kubota , Toshiyuki Sugimoto , Toshio Miyake
发明人： Shouji Ikegami , Michio Kubota , Toshiyuki Sugimoto , Toshio Miyake
IPC分类号： A23C9/123 , A21D2/18 , A23B4/02 , A23C9/152 , A23G3/34 , A23G3/48 , A23G4/00 , A23J3/16 , A23L1/19 , A23L1/202 , A23L1/236 , A23L1/30 , A23L1/32 , A23L2/39 , A61K8/04 , A61K8/60 , A61K8/72 , A61K8/73 , A61K31/70 , A61K36/25 , A61K38/00 , A61K47/26 , A61P3/00 , A61P17/00 , A61Q7/00 , A61Q11/00 , A61Q17/04 , A61Q19/02 , C07H3/04 , C12N9/24 , C12P19/12 , C12P19/14 , C12R1/01 , C12N1/20
CPC分类号： C12Y302/01141 , A61K8/60 , A61Q11/00 , A61Q17/04 , A61Q19/02 , A61Q7/00 , C12N9/2402 , C12P19/12 , C12P19/14 , Y10S435/822
摘要： Disclosed are novel thermostable trehalose-releasing enzyme, and its preparations and uses. The enzyme is obtainable from the culture of microorganisms such as Sulfolobus acidocaldarius (ATCC 33909 and ATCC 49426) and Sulfolobus solfataricus (ATCC 35091 and ATCC 35092), and capable of hydrolyzing at a temperature of over 55.degree. C. the linkage between a trehalose moiety and the remaining glycosyl moiety in a non-reducing saccharide having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher. Trehalose and compositions containing the same are extensively useful in food products, cosmetics and pharmaceuticals.
摘要翻译：公开了新型热稳定海藻糖释放酶及其制备和用途。该酶可从微生物的培养物获得，例如硫酸亚硫酸杆菌（ATCC 33909和ATCC 49426）和溶血S螨（Sulfolobus solfataricus）（ATCC 35091和ATCC 35092），并且能够在超过55℃的温度下水解，海藻糖部分并且具有海藻糖结构的非还原性糖中的剩余的糖基部分作为末端单元，并且具有3或更高的葡萄糖聚合度。海藻糖和含有它们的组合物在食品，化妆品和药物中广泛使用。

49. 发明授权

US6066477A Method of producing glucosylated saccharides with kojibiose phosphorylase 有权
标题翻译：用曲霉糖磷酸化酶生产葡糖基化糖的方法
公开(公告)号：US6066477A
公开(公告)日：2000-05-23
申请号：US188403
申请日：1998-11-10
申请人： Tomoyuki Nishimoto , Michio Kubota , Hiroto Chaen , Toshio Miyake
发明人： Tomoyuki Nishimoto , Michio Kubota , Hiroto Chaen , Toshio Miyake
IPC分类号： A61K8/60 , A61K38/00 , A61Q11/00 , A61Q17/04 , A61Q19/02 , C12N1/21 , C12N9/10 , C12N15/54 , C12P19/18 , C12P19/44 , C12P19/00
CPC分类号： A61K8/60 , A61Q11/00 , A61Q17/04 , A61Q19/02 , C12N9/1051 , C12P19/18 , A61K38/00
摘要： A kojibiose phosphorylase which hydrolyzes kojibiose in the presence of an inorganic phosphoric acid to form D-glucose and .beta.-D-glucose-1-phosphoric acid, forms kojibiose and an inorganic phosphoric acid from .beta.-D-glucose-1-phosphoric acid, and catalyzes the transfer reaction of glucosyl group to other saccharides using .beta.-D-glucose-1-phosphoric acid as a saccharide donor. The enzyme is obtainable from natural sources such as microorganisms of the genus Thermoanaerobium, and obtainable by recombinant technology.
摘要翻译：在无机磷酸存在下水解曲霉糖以形成D-葡萄糖和β-D-葡萄糖-1-磷酸的曲霉糖磷酸化酶，从β-D-葡萄糖-1-磷酸形成曲酸和无机磷酸，并使用β-D-葡萄糖-1-磷酸作为糖供体催化葡糖基转移到其他糖类上。该酶可从天然来源例如Thermoanaerobium属的微生物获得，可通过重组技术获得。

50. 发明授权

US06017899A Non-reducing saccharide-forming enzyme, its preparation and uses 失效
标题翻译：非还原性糖形成酶，其制备及用途
公开(公告)号：US06017899A
公开(公告)日：2000-01-25
申请号：US769143
申请日：1996-12-18
申请人： Kazuhiko Maruta , Michio Kubota , Toshiyuki Sugimoto , Toshio Miyake
发明人： Kazuhiko Maruta , Michio Kubota , Toshiyuki Sugimoto , Toshio Miyake
IPC分类号： A23L1/236 , A21D2/18 , A23C9/13 , A23C9/152 , A23G3/00 , A23G3/34 , A23G3/42 , A23G3/48 , A23G4/00 , A23G4/10 , A23J3/16 , A23L1/09 , A23L1/31 , A23L1/314 , A23L1/32 , A23L2/60 , A61K8/00 , A61K8/60 , A61K8/66 , A61K8/73 , A61K9/36 , A61K31/00 , A61K31/02 , A61K38/21 , A61K47/26 , A61Q11/00 , A61Q17/04 , A61Q19/00 , C07H20060101 , C07H1/00 , C07H1/06 , C07H3/00 , C07H3/04 , C07H3/06 , C07K7/00 , C08B37/00 , C12N1/20 , C12N9/24 , C12N9/26 , C12N9/90 , C12P19/00 , C12P19/12 , C12P19/14 , C12P19/24 , C12R1/41 , C13K20060101 , C13K13/00 , A61K31/70 , A23L1/03
CPC分类号： C13K13/00 , A23G3/343 , A23G3/346 , A23G3/42 , A23G4/10 , A23L13/428 , A23L15/20 , A23L27/33 , A61K8/60 , A61K8/66 , A61Q11/00 , A61Q19/00 , C07H1/00 , C07H3/04 , C12N9/90 , C12P19/12 , A23G2200/06 , A61Q17/04 , A61Q19/02 , A61Q7/00
摘要： Disclosed are novel non-reducing saccharide-forming enzyme, and its preparation and uses. The enzyme is obtainable from the culture of microorganisms such as Rhizobium sp. M-11 (FERM BP 4130) and Arthrobacter sp. Q36 (FERM BP-4316), and capable of forming non-reducing saccharides having a trehalose structure when allowed to act on reducing partial starch hydrolysates. Glucoamylase and .alpha.-glucosidase readily yield trehalose when allowed to act on the non-reducing saccharides. These non-reducing saccharides and trehalose are extensively useful in food products, cosmetics and pharmaceuticals.
摘要翻译：公开了新型非还原性糖形成酶及其制备和用途。该酶可从诸如根瘤菌属的微生物培养物获得。 M-11（FERM BP 4130）和节杆菌属 Q36（FERM BP-4316），并能够形成具有海藻糖结构的非还原性糖类，当其作用于还原部分淀粉水解产物时。葡糖淀粉酶和α-葡萄糖苷酶当允许作用于非还原糖时容易产生海藻糖。这些非还原性糖和海藻糖广泛用于食品，化妆品和药物。

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