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41. 发明授权

US5973231A Bacillus thuringiensis isolates, toxins, and genes for controlling certain coleopteran pests 失效
标题翻译：苏云金芽孢杆菌分离株，毒素和控制某些鞘翅目害虫的基因
公开(公告)号：US5973231A
公开(公告)日：1999-10-26
申请号：US76193
申请日：1998-05-12
申请人： Gregory A. Bradfisch , Judy Muller-Cohn , Kenneth E. Narva , Jenny M. Fu , Mark Thompson
发明人： Gregory A. Bradfisch , Judy Muller-Cohn , Kenneth E. Narva , Jenny M. Fu , Mark Thompson
IPC分类号： C07K14/325 , C12N1/21 , C12N15/32 , A01H5/00 , C12N5/04 , C12N15/82
CPC分类号： C07K14/325
摘要： The subject invention concerns materials and methods useful in the control of pests and, particularly, plant pests. More specifically, the subject invention concerns novel genes and pesticidal toxins referred to as 86A1(b) and 52A1(b). In preferred embodiments, the subject toxins are used for controlling flea beetles of the genus Phyllotreta. Using the genes described herein, the transformation of plants can be accomplished using techniques known to those skilled in the art. In addition, the subject invention provides toxin genes optimized for expression in plants.
摘要翻译：本发明涉及可用于防治害虫，特别是植物害虫的材料和方法。更具体地，本发明涉及被称为86A1（b）和52A1（b）的新基因和杀虫毒素。在优选的实施方案中，本发明的毒素用于控制Phyllotreta属的跳蚤甲虫。使用本文所述的基因，可以使用本领域技术人员已知的技术来实现植物的转化。此外，本发明提供了优化用于在植物中表达的毒素基因。

42. 发明授权

US5831011A Bacillus thuringiensis genes encoding nematode-active toxins 失效
公开(公告)号：US5831011A
公开(公告)日：1998-11-03
申请号：US590554
申请日：1996-03-21
申请人： Jewel Payne , Kenneth E. Narva , Jenny Fu
发明人： Jewel Payne , Kenneth E. Narva , Jenny Fu
IPC分类号： C12N15/09 , A01N37/46 , A01N63/00 , A01N63/02 , C07K14/325 , C12N5/10 , C12N15/31 , C07K14/32 , C12N1/20
CPC分类号： A01N37/46 , A01N63/00 , A01N63/02 , C07K14/325 , Y10S435/832
摘要： Novel B.t. genes encoding toxins active against nematode pests have been cloned. The DNA encoding the B.t. toxin can be used to transform various hosts to express the B.t. toxin.

43. 发明授权

US5635480A Bacillus thuringiensis isolates and toxins 失效
标题翻译：苏云金芽孢杆菌分离株和毒素
公开(公告)号：US5635480A
公开(公告)日：1997-06-03
申请号：US455313
申请日：1995-05-31
申请人： Jewel Payne , Kenneth E. Narva , Kendrick A. Uyeda , Christine J. Stalder , Tracy E. Michaels
发明人： Jewel Payne , Kenneth E. Narva , Kendrick A. Uyeda , Christine J. Stalder , Tracy E. Michaels
IPC分类号： C12N15/09 , A01N63/00 , A61K38/00 , C07K14/325 , C12N15/32 , C12P21/02 , C12R1/07 , C12R1/19
CPC分类号： A01N63/00 , C07K14/325 , C12R1/075 , A61K38/00
摘要： Disclosed and claimed are toxins produced by novel Bacillus thuringiensis isolates designated B.t. PS92J, B.t. PS196S1, B.t. PS201L1, and B.t. PS201T6, which have dipteran and/or corn rootworm activity. Thus, the isolates, or mutants thereof, can be used to control such pests. Further, claimed are novel genes encoding these .delta.-endotoxins, which can be expressed in other hosts. Expression of the .delta.-endotoxins in such hosts results in the control of susceptible insect pests in the environment of such hosts.
摘要翻译：披露和要求的是由命名为B.t.的新型苏云金芽孢杆菌分离株产生的毒素。 PS92J，B.t. PS196S1，B.t. PS201L1和B.t. PS201T6，其具有dipteran和/或玉米根虫活性。因此，分离物或其突变体可用于控制这些害虫。此外，要求的是编码这些δ-内毒素的新基因，其可以在其他宿主中表达。这种宿主中的δ-内毒素的表达导致对这种宿主环境中易感害虫的控制。

44. 发明授权

US5632987A Bacillus thuringiensis toxins active against corn rootworm larvae 失效
标题翻译：对玉米根虫幼虫有活性的苏云金芽孢杆菌毒素
公开(公告)号：US5632987A
公开(公告)日：1997-05-27
申请号：US357698
申请日：1994-12-16
申请人： Jewel Payne , Kenneth E. Narva
发明人： Jewel Payne , Kenneth E. Narva
IPC分类号： A01N63/00 , C07K14/325
CPC分类号： A01N63/00 , C07K14/325
摘要： Disclosed and claimed are toxins and genes from Bacillus thuringiensis strains designated PS80JJ1, PS158D5, PS167P, PS169E, PS177F1, PS177G, PS204G4, PS204G6 which can be used to control corn rootworm. Mutants which retain the activity of the parent strain can be used to control the pest. Further, isolated spores or purified toxins from these isolates can be used to control corn rootworm. Genes encoding .delta.-endotoxins can be removed from these strains using standard well-known techniques, and transferred to other hosts. Expression of the .delta.-endotoxin in such hosts results in control of corn rootworm larvae.
摘要翻译：披露和要求的是来自苏云金芽孢杆菌菌株的命名为PS80JJ1，PS158D5，PS167P，PS169E，PS177F1，PS177G，PS204G4，PS204G6的毒素和基因，其可以用于控制玉米根虫。保留亲本菌株活性的突变体可用于防治害虫。此外，这些分离物的分离的孢子或纯化的毒素可用于控制玉米根虫。可以使用标准的熟知技术从这些菌株中去除编码δ-内毒素的基因，并转移到其他宿主。这种宿主中的δ-内毒素的表达导致玉米根虫幼虫的控制。

45. 发明授权

US5322932A Nematode-active toxin from a Bacillus thuringiensis isolate 失效
标题翻译：来自苏云金芽孢杆菌分离株的线虫活性毒素
公开(公告)号：US5322932A
公开(公告)日：1994-06-21
申请号：US97798
申请日：1993-07-27
申请人： Kenneth E. Narva , George E. Schwab , Theresa Galasan , Jewel M. Payne
发明人： Kenneth E. Narva , George E. Schwab , Theresa Galasan , Jewel M. Payne
IPC分类号： A01N37/46 , A01N63/00 , A01N63/02 , C07K14/325 , C07K15/04
CPC分类号： A01N37/46 , A01N63/00 , A01N63/02 , C07K14/325 , C12R1/075
摘要： A novel B.t. gene encoding a toxin which is toxic to nematodes has been cloned from a B. thuringiensis microbe. The DNA encoding the B.t. toxin can be used to transform various prokaryotic and eukaryotic microbes to express the B.t. toxin. These recombinant microbes can be used to control nematodes in various environments.
摘要翻译：小说B.t. 从苏云金芽孢杆菌微生物克隆了对线虫有毒的毒素基因。编码B.t.的DNA 毒素可用于转化各种原核和真核微生物，以表达B.t. 毒素。这些重组微生物可用于控制各种环境中的线虫。

46. 发明授权

US5262399A Compositions and methods for the control of flukes 失效
标题翻译：用于控制吸虫的组成和方法
公开(公告)号：US5262399A
公开(公告)日：1993-11-16
申请号：US675772
申请日：1991-03-27
申请人： Leslie A. Hickle , August J. Sick , George E. Schwab , Kenneth E. Narva , Jewel M. Payne
发明人： Leslie A. Hickle , August J. Sick , George E. Schwab , Kenneth E. Narva , Jewel M. Payne
IPC分类号： A01N37/46 , A01N63/00 , A01N63/02 , C07K14/325 , A61K37/00 , A01N25/00
CPC分类号： C12R1/075 , A01N37/46 , A01N63/00 , A01N63/02 , C07K14/325
摘要： Methods and compositions for the control of flukes are described. Specifically, Bacillus thuringiensis (B.t.) isolates having flukicidal activity are disclosed. Also described are recombinant hosts which express B.t. genes coding for flukicidal toxins. The B.t. isolates and recombinant proteins are shown to be useful in a method for controlling flukes including the liver fluke.
摘要翻译：描述了用于控制吸虫的方法和组合物。具体来说，公开了具有氟杀菌活性的苏云金芽孢杆菌（B.t.）分离株。还描述了表达B.t.的重组宿主。编码流感毒素的基因。 B.t. 分离物和重组蛋白质显示可用于控制包括肝吸虫在内的吸虫的方法。

47. 发明授权

US09796983B2 Combinations including CRY3AA and CRY6AA proteins to prevent development of resistance in corn rootworms (Diabrotica spp.) 有权
公开(公告)号：US09796983B2
公开(公告)日：2017-10-24
申请号：US13643052
申请日：2011-04-22
申请人： Kenneth E. Narva , Thomas Meade , Kristin J. Fencil , Huarong Li , Timothy D. Hey , Aaron T. Woosley , Monica B. Olson
发明人： Kenneth E. Narva , Thomas Meade , Kristin J. Fencil , Huarong Li , Timothy D. Hey , Aaron T. Woosley , Monica B. Olson
IPC分类号： A01H5/10 , A01N25/00 , C12N15/82 , C07K14/195 , C07K14/325 , A01N37/18 , B65D85/00 , A01G1/00 , A01N43/50 , B32B1/02
CPC分类号： C12N15/8286 , A01G22/00 , A01H5/10 , A01N37/18 , A01N43/50 , B32B1/02 , B65D85/00 , C07K14/325 , Y02A40/162 , Y10T428/13
摘要： The subject invention relates in part to Cry3Aa in combination with Cry6Aa. The subject invention relates in part to the surprising discovery that combinations of Cry3Aa and Cry6Aa are useful for preventing development of resistance (to either insecticidal protein system alone) by a corn rootworm (Diabrotica spp.) population. Included within the subject invention are plants producing these insecticidal Cry proteins, which are useful to mitigate concern that a corn rootworm population could develop that would be resistant to either of these insecticidal protein systems alone. The subject invention also relates in part to combinations of Cry3Aa and Cry6Aa proteins “triple-stacked” or “multi-stacked” with another insecticidal protein(s) such as a Cry6Aa protein or binary Cry34/35 proteins. Thus, such embodiments target rootworms with three modes of action. Transgenic plants, including corn, comprising a cry6Aa gene and a cry3Aa gene are included within the scope of the subject invention.

48. 发明申请

US20110239334A1 NEMATODE-RESISTANT PLANTS, AND MODIFIED BACILLUS THURINGIENSIS CRY GENES AND PROTEINS 审中-公开
标题翻译：抗虫植物和改良的BACILUS THURINGIENSIS CRY基因和蛋白质
公开(公告)号：US20110239334A1
公开(公告)日：2011-09-29
申请号：US13060240
申请日：2009-08-25
申请人： Timothy D. Hey , Aaron T. Woosley , Kenneth E. Narva
发明人： Timothy D. Hey , Aaron T. Woosley , Kenneth E. Narva
IPC分类号： A01N37/18 , A01H5/00 , C12N15/32 , C07K14/325 , C12N5/04 , A01P5/00
CPC分类号： C12N15/8285 , Y02A40/164
摘要： The subject invention concerns plants protected from nematode feeding damage and improved versions of Cry proteins. Synthetic genes encoding Cry proteins are also part of the subject invention. Another embodiment of the subject invention includes plants transformed with the genes of the subject invention. In yet another embodiment the subject invention concerns Bt proteins for in-plant protection against crop damage by root knot nematode (RKN; Meloidogyne incognita) and soybean cyst nematode (SCN; Heterodera glycines).
摘要翻译：本发明涉及防止线虫喂养损伤和Cry蛋白质改良形式的植物。编码Cry蛋白的合成基因也是本发明的一部分。本发明的另一个实施方案包括用本发明的基因转化的植物。在另一个实施方案中，本发明涉及用于通过根结线虫（RKN;不同根结线虫）和大豆胞囊线虫（SCN;异源甘氨酸）对作物损害的植物内保护的Bt蛋白质。

49. 发明授权

US07129212B2 Polynucleotides, pesticidal proteins, and novel methods of using them 有权
标题翻译：多核苷酸，杀虫蛋白和使用它们的新方法
公开(公告)号：US07129212B2
公开(公告)日：2006-10-31
申请号：US10698096
申请日：2003-10-31
申请人： Kenneth E. Narva , Donald J. Merlo
发明人： Kenneth E. Narva , Donald J. Merlo
IPC分类号： A61K38/16 , C07K14/32
CPC分类号： C12Q1/689 , A01N63/00 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Disclosed and claimed are novel Bacillus thuringiensis isolates, pesticidal toxins, and genes. The subject invention also provides novel methods of controlling diamond back moths.
摘要翻译：公开和要求的是新型苏云金芽孢杆菌分离株，杀虫毒素和基因。本发明还提供了控制钻石背蛾的新方法。

50. 发明授权

US06673990B2 Plant-optimized genes encoding pesticidal chimeric cry protein toxins 有权
标题翻译：植物优化基因编码农药嵌合cry蛋白毒素
公开(公告)号：US06673990B2
公开(公告)日：2004-01-06
申请号：US09826660
申请日：2001-04-05
申请人： Guy A. Cardineau , Steven J. Stelman , Kenneth E. Narva
发明人： Guy A. Cardineau , Steven J. Stelman , Kenneth E. Narva
IPC分类号： A01H500
CPC分类号： C07K14/325 , C07K2319/00 , C12N15/8286 , Y02A40/162
摘要： The subject invention provides a DNA construct comprising a promoter operably linked to a nucleic acid encoding an N-terminal core toxin and a C-terminal Cry1Ab protoxin portion, wherein the nucleic acid encoding the C-terminal Cry1Ab protoxin portion is a plant optimized polynucleotide sequence.
摘要翻译：本发明提供了包含与编码N末端核心毒素和C-末端Cry1Ab原毒素部分的核酸可操作连接的启动子的DNA构建体，其中编码C末端Cry1Ab原毒素部分的核酸是植物优化的多核苷酸序列。

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