专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

41. 发明授权

US09771345B2 Coferons and methods of making and using them 有权
公开(公告)号：US09771345B2
公开(公告)日：2017-09-26
申请号：US13500857
申请日：2010-10-07
申请人： Francis Barany , Maneesh Pingle , Sarah Filippa Giardina , Donald Bergstrom , Lee Daniel Arnold
发明人： Francis Barany , Maneesh Pingle , Sarah Filippa Giardina , Donald Bergstrom , Lee Daniel Arnold
IPC分类号： A61K31/69 , C07D319/14 , C07D207/12 , C07D207/24 , C07D209/52 , C07D239/42 , C07D239/54 , C07D241/36 , C07D263/20 , C07D295/18 , C07D307/80 , C07D317/10 , C07D317/44 , C07D319/12 , C07D403/06 , C07D403/14 , C07D405/14 , C07D413/06 , C07D471/14 , C07D491/04 , C07D491/10 , C07D491/14 , C07F5/02 , C40B30/04
CPC分类号： C07D319/14 , C07D207/12 , C07D207/24 , C07D209/52 , C07D239/42 , C07D239/54 , C07D241/36 , C07D263/20 , C07D295/18 , C07D307/80 , C07D317/10 , C07D317/44 , C07D319/12 , C07D403/06 , C07D403/14 , C07D405/14 , C07D413/06 , C07D471/14 , C07D491/04 , C07D491/10 , C07D491/14 , C07F5/025 , C40B30/04
摘要： The present invention is directed to a monomer useful in preparing therapeutic compounds. The monomer includes one or more pharmacophores which potentially binds to a target molecule with a dissociation constant of less than 300 μM and a linker element connected to the pharmacophore. The linker element has a molecular weight less than 500 daltons, is connected, directly or indirectly through a connector, to the pharmacophore.

42. 发明授权

US09090941B2 Use of lecithin:retinol acyl transferase gene promoter methylation in evaluating the cancer state of subject 有权
标题翻译：使用卵磷脂：视黄醇酰基转移酶基因启动子甲基化评估受试者的癌症状态
公开(公告)号：US09090941B2
公开(公告)日：2015-07-28
申请号：US12520386
申请日：2007-12-19
申请人： Francis Barany , Yu-Wei Cheng , Philip Paty , Daniel Notterman
发明人： Francis Barany , Yu-Wei Cheng , Philip Paty , Daniel Notterman
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02
CPC分类号： C12Q1/6886 , C12Q2600/106 , C12Q2600/118 , C12Q2600/154 , C12Q2600/16
摘要： The present invention relates to a method of evaluating the cancer state of a subject using lecithin:retinol acyl transferase (LRAT) gene promoter methylation status. Methods of analyzing and quantifying LRAT gene promoter methylation level are also disclosed. The present invention also relates to methods of determining the prognosis for s subject having cancer by assessing LRAT mRNA expression and LRAT protein expression. Methods of cancer detection, diagnosis, prognosis, and treatment are also disclosed.
摘要翻译：本发明涉及使用卵磷脂：视黄醇酰基转移酶（LRAT）基因启动子甲基化状态评价受试者的癌症状态的方法。还公开了分析和定量LRAT基因启动子甲基化水平的方法。本发明还涉及通过评估LRAT mRNA表达和LRAT蛋白表达来测定患有癌症的受试者的预后的方法。还公开了癌症检测，诊断，预后和治疗方法。

43. 发明授权

US08853185B2 Coferons and methods of making and using them 有权
标题翻译：咖啡因及其使用方法
公开(公告)号：US08853185B2
公开(公告)日：2014-10-07
申请号：US12937053
申请日：2009-04-09
申请人： Francis Barany , Maneesh Pingle , Donald Bergstrom , Sarah Filippa Giardina
发明人： Francis Barany , Maneesh Pingle , Donald Bergstrom , Sarah Filippa Giardina
IPC分类号： A61K31/69 , C07F5/02 , C40B30/04 , C40B50/04
CPC分类号： A61K47/545 , A61K31/69 , C07D233/64 , C07D307/92 , C07D309/30 , C07D319/24 , C07D493/22 , C07F5/02 , C07H7/02 , C07H9/02 , C40B30/04
摘要： A monomer useful in prepaπng therapeutic compounds includes a diversity element which potentially binds to a target molecule with a dissociation constant of less than 300 11 M and a linker element connected to the diversity element The linker element has a molecular weight less than 500 daltons, is connected, directly or indirectly through a connector, to said diversity element, and is capable of forming a reversible covalent bond or noncovalent interaction with a binding partner of the linker element The monomers can be covalently or non-covalently linked together to form a therapeutic multimer or a precursor thereof.
摘要翻译：可用于制备治疗化合物的单体包括潜在地结合靶分子的多样性元件，其解离常数小于300±11M，连接元件连接到多样性元件。连接体元件具有小于500道尔顿的分子量通过连接器直接或间接连接到所述多样性元件，并且能够与连接体元件的结合配偶体形成可逆共价键或非共价相互作用。所述单体可以共价或非共价连接在一起形成治疗性多聚体或其前体。

44. 发明授权

US08597891B2 Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions 失效
公开(公告)号：US08597891B2
公开(公告)日：2013-12-03
申请号：US13543365
申请日：2012-07-06
申请人： Francis Barany , Matthew Lubin , George Barany , Robert P. Hammer
发明人： Francis Barany , Matthew Lubin , George Barany , Robert P. Hammer
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6876 , C12Q1/6813 , C12Q1/6827 , C12Q1/683 , C12Q1/6851 , C12Q1/6853 , C12Q1/6858 , C12Q1/686 , C12Q1/6862 , C12Q1/6869 , C12Q1/6881 , C12Q2600/156 , C12Q2600/16 , C12Q2561/125 , C12Q2531/113 , C12Q2525/161 , C12Q2545/114 , C12Q2535/131 , C12Q2521/501 , C12Q2537/143 , C12Q2533/107 , C12Q2525/131 , C12Q2525/125 , C12Q2521/319 , C12Q2521/531 , C12Q2525/155
摘要： The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligation detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence difference.

45. 发明申请

US20110136116A1 DETECTION OF TARGET NUCLEIC ACID SEQUENCES USING FLUORESCENCE RESONANCE ENERGY TRANSFER 审中-公开
标题翻译：使用荧光共振能量转移检测目标核酸序列
公开(公告)号：US20110136116A1
公开(公告)日：2011-06-09
申请号：US12934866
申请日：2009-04-08
申请人： Francis Barany , Maneesh Pingle , Donald Bergstrom
发明人： Francis Barany , Maneesh Pingle , Donald Bergstrom
IPC分类号： C12Q1/68 , G01N33/53
CPC分类号： C12Q1/6827 , Y10T436/143333 , C12Q2561/125 , C12Q2527/107 , C12Q2525/301
摘要： A method for identifying a plurality of target nucleic acid molecules in a sample. The method provides a plurality of oligonucleotide probe sets. Each set comprises a first and a second probe, each having a target-specific portion and a tunable portion with an acceptor or a donor group. The first probe further comprises an endcapped hairpin. A reaction comprises a denaturation and hybridization cycle. Under the hybridization, the set of probes hybridize in a base-specific manner to their respective target nucleotide sequences, and ligate to one another to form a ligation product. Under conditions that permit hybridization of the tunable portions of the ligation product to one another, an internally hybridized ligation product formed, which allows the detection of the fluorescence resonance energy transfer (FRET). A method comprising PCR amplification is also disclosed.
摘要翻译：用于鉴定样品中多个靶核酸分子的方法。该方法提供多个寡核苷酸探针组。每组包括第一和第二探针，每个具有目标特异性部分和具有受体或供体基团的可调部分。第一探针还包含封端的发夹。反应包括变性和杂交循环。在杂交下，探针组以碱基特异性方式与其各自的靶核苷酸序列杂交，并且彼此连接以形成连接产物。在允许连接产物的可调部分彼此杂交的条件下，形成内部杂交的连接产物，其允许检测荧光共振能量转移（FRET）。还公开了包括PCR扩增的方法。

46. 发明授权

US07556924B2 Detection of nucleic acid sequence differences using coupled ligase detection with padlock probes and polymerase chain reactions 有权
标题翻译：使用挂锁探针和聚合酶链反应的偶联连接酶检测检测核酸序列差异
公开(公告)号：US07556924B2
公开(公告)日：2009-07-07
申请号：US11931403
申请日：2007-10-31
申请人： Francis Barany , Matthew Lubin , George Barany , Robert P. Hammer
发明人： Francis Barany , Matthew Lubin , George Barany , Robert P. Hammer
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6876 , C12Q1/6813 , C12Q1/6827 , C12Q1/683 , C12Q1/6851 , C12Q1/6853 , C12Q1/6858 , C12Q1/686 , C12Q1/6862 , C12Q1/6869 , C12Q1/6881 , C12Q2600/156 , C12Q2600/16 , C12Q2561/125 , C12Q2531/113 , C12Q2525/161 , C12Q2545/114 , C12Q2535/131 , C12Q2521/501 , C12Q2537/143 , C12Q2533/107 , C12Q2525/131 , C12Q2525/125 , C12Q2521/319 , C12Q2521/531 , C12Q2525/155
摘要： The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligase detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.
摘要翻译：本发明涉及鉴定靶核苷酸序列的方法。该方法包括在连接酶检测反应混合物中的靶核苷酸序列上形成连接产物，扩增连接产物以在聚合酶链式反应（PCR）混合物中形成扩增的连接产物，检测扩增的连接产物，并鉴定靶核苷酸序列。连接酶检测反应和聚合酶链式反应的这种偶联允许核酸序列差异的多重检测。

47. 发明授权

US07364858B2 Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions 有权
公开(公告)号：US07364858B2
公开(公告)日：2008-04-29
申请号：US11590529
申请日：2006-10-31
申请人： Francis Barany , Matthew Lubin , George Barany , Robert P. Hammer
发明人： Francis Barany , Matthew Lubin , George Barany , Robert P. Hammer
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6876 , C12Q1/6813 , C12Q1/6827 , C12Q1/683 , C12Q1/6851 , C12Q1/6853 , C12Q1/6858 , C12Q1/686 , C12Q1/6862 , C12Q1/6869 , C12Q1/6881 , C12Q2600/156 , C12Q2600/16 , C12Q2561/125 , C12Q2531/113 , C12Q2525/161 , C12Q2545/114 , C12Q2535/131 , C12Q2521/501 , C12Q2537/143 , C12Q2533/107 , C12Q2525/131 , C12Q2525/125 , C12Q2521/319 , C12Q2521/531 , C12Q2525/155
摘要： The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligase detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.

48. 发明授权

US07332285B2 Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions 有权
公开(公告)号：US07332285B2
公开(公告)日：2008-02-19
申请号：US11590383
申请日：2006-10-31
申请人： Francis Barany , Matthew Lubin , George Barany , Robert P. Hammer
发明人： Francis Barany , Matthew Lubin , George Barany , Robert P. Hammer
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6876 , C12Q1/6813 , C12Q1/6827 , C12Q1/683 , C12Q1/6851 , C12Q1/6853 , C12Q1/6858 , C12Q1/686 , C12Q1/6862 , C12Q1/6869 , C12Q1/6881 , C12Q2600/156 , C12Q2600/16 , C12Q2561/125 , C12Q2531/113 , C12Q2525/161 , C12Q2545/114 , C12Q2535/131 , C12Q2521/501 , C12Q2537/143 , C12Q2533/107 , C12Q2525/131 , C12Q2525/125 , C12Q2521/319 , C12Q2521/531 , C12Q2525/155
摘要： The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligase detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.

49. 发明申请

US20060024731A1 Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions 有权
标题翻译：使用偶联连接酶检测和聚合酶链反应检测核酸序列差异
公开(公告)号：US20060024731A1
公开(公告)日：2006-02-02
申请号：US11229366
申请日：2005-09-16
申请人： Francis Barany , Matthew Lubin , George Barany , Robert Hammer
发明人： Francis Barany , Matthew Lubin , George Barany , Robert Hammer
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6876 , C12Q1/6813 , C12Q1/6827 , C12Q1/683 , C12Q1/6851 , C12Q1/6853 , C12Q1/6858 , C12Q1/686 , C12Q1/6862 , C12Q1/6869 , C12Q1/6881 , C12Q2600/156 , C12Q2600/16 , C12Q2561/125 , C12Q2531/113 , C12Q2525/161 , C12Q2545/114 , C12Q2535/131 , C12Q2521/501 , C12Q2537/143 , C12Q2533/107 , C12Q2525/131 , C12Q2525/125 , C12Q2521/319 , C12Q2521/531 , C12Q2525/155
摘要： The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligase detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.
摘要翻译：本发明涉及鉴定靶核苷酸序列的方法。该方法包括在连接酶检测反应混合物中的靶核苷酸序列上形成连接产物，扩增连接产物以在聚合酶链式反应（PCR）混合物中形成扩增的连接产物，检测扩增的连接产物，并鉴定靶核苷酸序列。连接酶检测反应和聚合酶链式反应的这种偶联允许核酸序列差异的多重检测。

50. 发明申请

US20050266487A1 High fidelity thermostable ligase and uses thereof 有权
标题翻译：高保真热稳定连接酶及其用途
公开(公告)号：US20050266487A1
公开(公告)日：2005-12-01
申请号：US11167048
申请日：2005-06-24
申请人： Francis Barany , Weiguo Cao , Jie Tong
发明人： Francis Barany , Weiguo Cao , Jie Tong
IPC分类号： C07H21/04 , C12N9/00 , C12N9/22 , C12Q1/68
CPC分类号： C12N9/93
摘要： The present invention is directed to a mutant thermostable ligase having substantially higher fidelity than either T4 ligase or Thermus thermophilus ligase. The ligase of the present invention is a mutant of a wild-type thermostable ligase having a histidine adjacent a KXDG motif, where the mutant thermostable ligase has a mutation in its amino sequence where the histidine adjacent the KXDG motif in the wild-type thermostable ligase is replaced with an arginine, and wherein X is any amino acid. The DNA molecule encoding this enzyme as well as expression systems and host cells containing it are also disclosed. The thermostable ligase of the present invention is useful in carrying out a ligase detection reaction process and a ligase chain reaction process.
摘要翻译：本发明涉及一种具有比T4连接酶或Thermus thermophilus连接酶具有更高保真度的突变热稳定连接酶。本发明的连接酶是具有与KXDG基序相邻的组氨酸的野生型热稳定连接酶的突变体，其中突变热稳定连接酶在其氨基序列中具有突变，其中野生型热稳定连接酶中KXDG基序相邻的组氨酸被精氨酸代替，其中X是任何氨基酸。还公开了编码该酶的DNA分子以及含有它的表达系统和宿主细胞。本发明的热稳定连接酶可用于进行连接酶检测反应过程和连接酶链反应过程。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式