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41. 发明公开

EP1633763A1 PROCESS FOR THE CONCENTRATION AND/OR ISOLATION OF NUCLEIC ACID OR NUCLEIC ACID-CONTAINING SPECIES 审中-公开
标题翻译：处理该浓度和/或隔离NUCLEIC或含核酸样品
公开(公告)号：EP1633763A1
公开(公告)日：2006-03-15
申请号：EP04735881.7
申请日：2004-06-03
申请人： Qiagen AS , QIAGEN GmbH
发明人： DEGGERDAL, Arne , REITAN, Evy, H. , SKAGESTAD, Vidar
IPC分类号： C07H1/08 , C12N15/10
CPC分类号： C12N15/1003
摘要： The present invention relates to a process for the concentration and/or isolation of nucleic acids or nucleic acid-containing species from a nucleic acid-containing solution, and a kit therefor. In one embodiment, the invention relates to the concentration and/or isolation of DNA and RNA from nucleic acid-containing solutions.

42. 发明公开

EP1756299A1 NUCLEIC ACID SEQUENCING 审中-公开
标题翻译：核酸测序
公开(公告)号：EP1756299A1
公开(公告)日：2007-02-28
申请号：EP05718259.4
申请日：2005-03-24
申请人： Qiagen AS
发明人： LARSEN, Frank
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858 , C12Q1/6827 , C12Q1/6869 , C12Q2535/125 , C12Q2565/301
摘要： A method for determining a target nucleic acid sequence, wherein the target nucleic acid sequence is comprised in a preparation comprising a non-target nucleic acid sequence, the target nucleic acid sequence and the non-target nucleic acid sequence each having a first region of common sequence upstream of a first region of dissimilar sequence upstream of a second region of dissimilar sequence, the method comprising: (a) contacting the preparation with an oligonucleotide primer complementary to at least a portion of the first region of common sequence, under conditions to hybridise the primer thereto; and (b) subjecting the preparation to a sequencing reaction, such that the sequencing reaction proceeds into the second region of dissimilar sequence of the target nucleic acid sequence, thereby determining at least the second region of dissimilar sequence -of the target-nucleic acid- sequence; and wherein the method further comprises a step of blocking the sequencing reaction between the primer and the non-target nucleic acid sequence, such that the sequencing reaction does not proceed into the second region of dissimilar sequence of the nontarget nucleic acid sequence.
摘要翻译：一种确定靶核酸序列的方法，其中所述靶核酸序列包含在包含非靶核酸序列的制剂中，所述靶核酸序列和所述非靶核酸序列各自具有第一共同区域所述方法包括：（a）使所述制备物与寡核苷酸引物接触，所述寡核苷酸引物与所述第一共同序列区域的至少一部分互补，在杂交条件下接触所述第一区域的不同序列的第一区域的上游序列，引物; 和（b）使制备物经受测序反应，使得测序反应进行到靶核酸序列的不同序列的第二区域中，由此确定靶核酸序列的至少不同序列的第二区域，序列; 并且其中所述方法还包括阻断所述引物和所述非靶核酸序列之间的测序反应的步骤，使得所述测序反应不进行到所述非靶核酸序列的不同序列的第二区域。

43. 发明授权

EP1404442B1 PREPARATION OF POLYMER PARTICLES 有权
标题翻译：生产的聚合物颗粒
公开(公告)号：EP1404442B1
公开(公告)日：2006-03-08
申请号：EP02741063.8
申请日：2002-07-04
申请人： Qiagen AS
发明人： SKAGESTAD, Vidar , KILAAS, Lars, Particle Solution AS
IPC分类号： B01J20/30
CPC分类号： B01J20/28009 , B01J20/26 , B01J20/30 , C08F2/32 , C08F2/44 , Y10T428/254
摘要： A process for the preparation of polymer magnetic particles, which comprises: (a) providing a water phase containing magnetic components homogeneously dispersed therein; (b) wherein the water phase is contacted with or further contains a polymerisable metal-containing or organic monomer which is soluble in the water phase; and (c) polymerising the monomer in the presence of the magnetic in which the magnetic components are substantially uniformly distributed; wherein at least a part of the polymerising step (c) is carried out in a water-in-oil emulsion in which the water phase containing the magnetic components homogeneously dispersed therein is present as a discontinuous phase in a continuous oil phase.

44. 发明授权

EP1492804B1 PROCESS FOR ISOLATING NUCLEIC ACID WITH CHAOTROPE AGENTS AND AMMONIUM COMPOUNDS 有权
标题翻译：方法REINUNG使用离液和AMMONIUMREAGENTIEN核酸
公开(公告)号：EP1492804B1
公开(公告)日：2005-12-28
申请号：EP03712478.1
申请日：2003-04-02
申请人： Qiagen AS
发明人： REITAN, Evy , DEGGERDAL, Arne , SKAGESTAD, Vidar
IPC分类号： C07H21/00 , G01N33/553 , C01B21/068 , C12N1/08 , C12N15/10 , C12Q1/70 , C12Q1/68
CPC分类号： C12N15/1006
摘要： A process for isolating nucleic acid from a nucleic acid-containing sample, which comprises: (a) providing a chaotrope; (b) providing a nucleic acid binding solid phase capable of binding nucleic acid in the presence of the chaotrope; (c) providing a source of NH4+ or NH3; (d) contacting the sample with the nucleic acid binding solid phase in the presence of a liquid phase comprising the chaotrope and the NH4+ or NH3; and (e) optionally separating the solid phase with the nucleic acid bound thereto from the liquid phase.

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