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    • 41. 发明申请
    • Optical Switch Using Rare Earth Doped Glass
    • 使用稀土掺杂玻璃的光开关
    • US20080107376A1
    • 2008-05-08
    • US11968860
    • 2008-01-03
    • Falgun PatelJeffrey Miller
    • Falgun PatelJeffrey Miller
    • G02B6/26
    • H01S3/06754H01S3/10007
    • The present invention provides an optical switch including a loss element having a signal loss, and a rare earth doped gain element optically connected in series with the loss element. The rare earth doped gain element is operable to produce a signal gain. The signal gain and the signal loss are about equal. The present invention also provides a method of optical switching including optically connecting a loss element in series with a rare earth doped gain element and passing an optical signal through the loss element and the gain element. The loss element attenuates the optical signal by a first amount. The method further includes selectively applying an optical pump to the gain element to perform the switching, the gain element amplifying the optical signal by the first amount in response to the optical pump.
    • 本发明提供了一种光开关,其包括具有信号损耗的损耗元件和与损耗元件串联光学连接的稀土掺杂增益元件。 稀土掺杂增益元件可操作以产生信号增益。 信号增益和信号损耗大致相等。 本发明还提供一种光学切换的方法,包括将稀土元素与稀土掺杂的增益元件串联光学连接并使光信号通过损耗元件和增益元件。 损耗元件将光信号衰减第一量。 该方法还包括选择性地将光泵施加到增益元件以执行切换,增益元件响应光泵将光信号放大第一量。
    • 44. 发明申请
    • Methods and compositions for targeted cleavage and recombination
    • 用于靶向切割和重组的方法和组合物
    • US20070218528A1
    • 2007-09-20
    • US10587723
    • 2005-02-03
    • Jeffrey Miller
    • Jeffrey Miller
    • C12P19/34C12P21/06C12N9/22
    • C12N15/10C07K14/4702C12N9/22
    • Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain, as well as polynucleotides encoding same. Fusion proteins comprising cleavage half-domains are used in pairs, to reconstitute a functional cleavage domain. In these fusion proteins, the zinc finger domain can be N-terminal to the cleavage half-domain, or the cleavage half-domain can be N-terminal to the zinc finger domain. The availability of fusion endonucleases having these different polarities allows targeting (and thereby binding) of zinc finger endonucleases either to opposite strands of the DNA target or to the same strand of the DNA target, thereby increasing the number of possible sequences which can be targeted and cleaved by the fusion proteins.
    • 本文公开了用于基因组序列的靶向切割,基因组序列的靶向改变以及基因组区域和与该基因组区域同源的外源多核苷酸之间的靶向重组的方法和组合物。 组合物包括包含切割结构域(或切割半结构域)和工程化锌指结构域的融合蛋白,以及编码它们的多核苷酸。 包含切割半结构域的融合蛋白成对使用,以重构功能性切割结构域。 在这些融合蛋白中,锌指结构域可以是切割半结构域的N末端,或者切割半结构域可以是锌指结构域的N末端。 具有这些不同极性的融合核酸内切酶的可得性允许锌指核酸内切酶与DNA靶的相对链或DNA靶的相同链靶向(从而结合),从而增加可靶向的可能序列的数目, 被融合蛋白切割。