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41. 发明申请

US20080070241A1 Kits for amplifying and detecting nucleic acid sequences 有权
标题翻译：用于扩增和检测核酸序列的试剂盒
公开(公告)号：US20080070241A1
公开(公告)日：2008-03-20
申请号：US11256592
申请日：2005-10-20
申请人： Elazar Rabbani , Jannis G. Stavrianopoulos , James J. Donegan , Jack Coleman , Marleen Walner
发明人： Elazar Rabbani , Jannis G. Stavrianopoulos , James J. Donegan , Jack Coleman , Marleen Walner
IPC分类号： C12Q1/68 , C07H21/00 , C12N9/00 , C12N9/10
CPC分类号： C12Q1/6844 , C12Q1/6853 , C12Q2531/119 , C12Q2525/301 , C12Q2525/161
摘要： This invention provides novel processes for amplifying nucleic acid sequences of interest, including linear and non-linear amplification. In linear amplification, a single initial primer or nucleic acid construct is utilized to carry out the amplification process. In non-linear amplification, a first initial primer or nucleic acid construct is employed with a subsequent initial primer or nucleic acid construct. In other non-linear amplification processes provided by this invention, a first initial primer or nucleic acid construct is deployed with a second initial primer or nucleic acid construct to amplify the specific nucleic acid sequence of interest and its complement that are provided. A singular primer or a singular nucleic acid construct capable of non-linear amplification can also be used to carry out non-linear amplification in accordance with this invention. Post-termination labeling process for nucleic acid sequencing is also disclosed in this invention that is based upon the detection of tagged molecules that are covalently bound to chemically reactive groups provided for chain terminators. A process for producing nucleic acid sequences having decreased thermodynamic stability to complementary sequences is also provided and achieved by this invention. Unique nucleic acid polymers are also disclosed and provided in addition to other novel compositions, kits and the like.
摘要翻译：本发明提供用于扩增感兴趣的核酸序列的新方法，包括线性和非线性扩增。在线性扩增中，使用单个初始引物或核酸构建体进行扩增过程。在非线性扩增中，使用第一初始引物或核酸构建体与随后的初始引物或核酸构建体。在本发明提供的其它非线性扩增方法中，用第二初始引物或核酸构建物展开第一个初始引物或核酸构建体，以扩增所提供的特异性核酸序列及其互补序列。也可以使用能够进行非线性扩增的单一引物或单个核酸构建体来进行本发明的非线性扩增。用于核酸测序的终止后标记方法也在本发明中公开，其基于共价结合到为链终止剂提供的化学反应性基团的标记分子的检测。通过本发明提供并实现了对互补序列产生具有降低的热力学稳定性的核酸序列的方法。除了其它新型组合物，试剂盒等之外，还公开并提供了独特的核酸聚合物。

42. 发明授权

US5013831A Detectable molecules, method of preparation and use 失效
标题翻译：可检测分子，制备和使用方法
公开(公告)号：US5013831A
公开(公告)日：1991-05-07
申请号：US521762
申请日：1990-05-08
申请人： Jannis G. Stavrianopoulos
发明人： Jannis G. Stavrianopoulos
IPC分类号： A61K51/04 , A61K51/10 , G01N33/533 , G01N33/553
CPC分类号： G01N33/533 , A61K51/0497 , A61K51/1093 , G01N33/553
摘要： A detectable molecule of the formulaA.sup.3 --(--X--R.sup.1 --E--Det.sup.b).sub.mwhere A.sup.3 is A.sup.2 or a polymer, where A.sup.3 has at least one modifiable reactive group selected from the group consisting of amino, hydroxy, cis OH, halides, aryl, imidazoyl, carbonyl, carboxy, thiol or a residue comprising an activated carbon; --X-- is selected from the group consisting of ##STR1## a C.sub.1 -C.sub.10 branched or unbranched alkyl or aralkyl, which may be substituted by --OH; --Y-- is a direct bond to --E--, or --Y-- is --E--R.sup.2 -- where R.sup.2 is a C.sub.1 -C.sub.10 branched or unbranched alkyl; Z.sub.a is chlorine, bromine or iodine; E is O, NH or an acyclic divalent sulfur atom; Det.sup.b is a chemical moiety capable of being detected, preferably comprising biotin or a metal chelator of the formula: ##STR2## or the 4-hydroxy or acyloxy derivative thereof, where R.sup.3 is C.sub.1 -C.sub.4 alkyl or CH.sub.2 COOM, M is the same or different and selected from the group consisting of hydrogen, a metal or non-metal cation or is C.sub.1 -C.sub.10 alkyl, aryl or aralkyl; and m is an integer from 1 to the total number of modified reactive groups on A.sup.3. The detectable molecules are useful in in vitro or in vivo assays or therapy.
摘要翻译：式A3-（ - X-R1-E-Detb）m的可检测分子，其中A3是A2或聚合物，其中A3具有选自氨基，羟基，顺式OH，卤化物中的至少一个可修饰的反应性基团，芳基，咪唑基，羰基，羧基，硫醇或包含活性炭的残基; -X-选自可以被-OH取代的C1-C10支链或非支链烷基或芳烷基组成的组 -Y-是与-E-或-Y-是-E-R 2 - 的直接键合，其中R 2是C 1 -C 10支链或非支链烷基; Za是氯，溴或碘; E是O，NH或无环二价硫原子; Detb是能够被检测的化学部分，优选包含下式的生物素或金属螯合剂：其中R 3是C 1 -C 4烷基或CH 2 COOM，M相同或不同并且选自氢，金属或非金属阳离子或是C 1 -C 10烷基，芳基或芳烷基; 并且m是从1到A3上修饰的反应性基团的总数的整数。可检测的分子可用于体外或体内测定或治疗。

43. 发明授权

US5002885A Detectable molecules, method preparation and use 失效
标题翻译：可检测分子，方法制备和使用
公开(公告)号：US5002885A
公开(公告)日：1991-03-26
申请号：US513723
申请日：1990-04-24
申请人： Jannis G. Stavrianopoulos
发明人： Jannis G. Stavrianopoulos
IPC分类号： A61K51/04 , A61K51/10 , G01N33/533 , G01N33/553
CPC分类号： A61K51/1093 , A61K51/0497 , G01N33/533 , G01N33/553
摘要： A detectable molecule of the formulaA.sup.3 --(--X--R.sup.1 --E--Det.sup.b).sub.mwhere A.sup.3 is A.sup.2 or a polymer, where A.sup.3 has at least one modifiable reactive group selected from the group consisting of amino, hydroxy, cis OH, halides, aryl, imidazoyl, carbonyl, carboxy, thiol or a residue comprising an activated carbon; --X-- is selected from the group consisting of ##STR1## a C.sub.1 -C.sub.10 branched or unbranched alkyl or aralkyl, which may be substituted by --OH; --Y-- is a direct bond to --E--, or --Y-- is --E--R.sup.2 -- where R.sup.2 is a C.sub.1 -C.sub.10 branched or unbranched alkyl; Z.sub.a is chlorine, bromine or iodine; E is O, NH or an acylic divalent sulfur atom; Det.sup.b is a chemical moiety capable of being detected, preferably comprising biotin or a metal chelator of the formula: ##STR2## or the 4-hydroxy or acyloxy derivative thereof, where R.sup.3 is C.sub.1 -C.sub.4 alkyl or CH.sub.2 COOM, M is the same or different and selected from the group consisting of hydrogen, a metal or non-metal cation or is C.sub.1 -C.sub.10 alkyl, aryl or aralkyl; and m is an integer from 1 to the total number of modified reactive groups on A.sup.3. The detectable molecules are useful in in vitro or in vivo assays or therapy.
摘要翻译：式A3-（ - X-R1-E-Detb）m的可检测分子，其中A3是A2或聚合物，其中A3具有选自氨基，羟基，顺式OH，卤化物中的至少一个可修饰的反应性基团，芳基，咪唑基，羰基，羧基，硫醇或包含活性炭的残基; -X-选自由以下组成的组：图像可以由以下组成的组：可以被 - 哦; -Y-是与-E-或-Y-是-E-R 2 - 的直接键合，其中R 2是C 1 -C 10支链或非支链烷基; Za是氯，溴或碘; E是O，NH或酰基二价硫原子; Detb是能够被检测的化学部分，优选包含下式的生物素或金属螯合剂：其中R 3是C 1 -C 4烷基或CH 2 COOM，M相同或不同并且选自氢，金属或非金属阳离子或是C 1 -C 10烷基，芳基或芳烷基; 并且m是从1到A3上修饰的反应性基团的总数的整数。可检测的分子可用于体外或体内测定或治疗。

44. 发明授权

US4952685A Detectable molecules, method of preparation and use 失效
标题翻译：可检测分子，制备和使用方法
公开(公告)号：US4952685A
公开(公告)日：1990-08-28
申请号：US43668
申请日：1987-04-28
申请人： Jannis G. Stavrianopoulos
发明人： Jannis G. Stavrianopoulos
IPC分类号： A61K51/04 , A61K51/10 , G01N33/533 , G01N33/553
CPC分类号： G01N33/553 , A61K51/0497 , A61K51/1093 , G01N33/533
摘要： A detectable molecule of the formulaA.sup.3 --(--X--R.sup.1 --E--Det.sup.b).sub.mwhere A.sup.3 is A.sup.2 or a polymer, where A.sup.3 has at least one modifiable reactive group selected from the group consisting of amino, hydroxy, cis OH, halides, aryl, imidazoyl, carbonyl, carboxy, thiol or a residue comprising an activated carbon; --X-- is selected from the group consisting of ##STR1## a C.sub.1 -C.sub.10 branched or unbranched alkyl or aralkyl, which may be substituted by --OH; --Y-- is a direct bond to --E--, or --Y-- is --E--R.sup.2 -- where R.sup.2 is a C.sub.1 -C.sub.10 branched or unbranched alkyl; Z.sub.a is chlorine, bromine or iodine; E is O, NH or an acyclic divalent sulfur atom; Det.sup.b is a chemical moiety capable of being detected, preferably comprising biotin or a metal chelator of the formula: ##STR2## or the 4-hydroxy or acyloxy derivative thereof, where R.sup.3 is C.sub.1 -C.sub.4 alkyl or CH.sub.2 COOM, M is the same or different and selected from the group consisting of hydrogen, a metal or non-metal cation or is C.sub.1 -C.sub.10 alkyl, aryl or aralkyl; and m is an integer from 1 to the total number of modified reactive groups on A.sup.3. The detectable molecules are useful in vitro or in vivo assays or therapy.
摘要翻译：式A3-（ - X-R1-E-Detb）m的可检测分子，其中A3是A2或聚合物，其中A3具有选自氨基，羟基，顺式OH，卤化物中的至少一个可修饰的反应性基团，芳基，咪唑基，羰基，羧基，硫醇或包含活性炭的残基; -X-选自可以被-OH取代的C1-C10支链或非支链烷基或芳烷基组成的组 + TR -Y-是与-E-或-Y-是-E-R 2 - 的直接键合，其中R 2是C 1 -C 10支链或非支链烷基; Za是氯，溴或碘; E是O，NH或无环二价硫原子; Detb是能够被检测的化学部分，优选包含下式的生物素或金属螯合剂：其中R 3是C 1 -C 4烷基或CH 2 COOM，M相同或不同并且选自氢，金属或非金属阳离子或是C 1 -C 10烷基，芳基或芳烷基; 并且m是从1到A3上修饰的反应性基团的总数的整数。可检测的分子在体外或体内测定或治疗中是有用的。

45. 发明授权

US4707440A Nucleic acid hybridization assay and detectable molecules useful in such assay 失效
标题翻译：核酸杂交测定和可用于此类测定的可检测分子
公开(公告)号：US4707440A
公开(公告)日：1987-11-17
申请号：US575396
申请日：1984-01-30
申请人： Jannis G. Stavrianopoulos
发明人： Jannis G. Stavrianopoulos
IPC分类号： C08F8/00 , A61K39/00 , A61K39/395 , A61K51/00 , A61K51/04 , A61K51/10 , B01J45/00 , C07C67/00 , C07C227/00 , C07C227/16 , C07C229/16 , C07C231/00 , C07C233/01 , C07C233/64 , C07C245/02 , C07C245/24 , C07C251/02 , C07C271/06 , C07C309/63 , C07C311/01 , C07C311/15 , C07C313/00 , C07C323/30 , C07C323/35 , C07C323/43 , C07C323/48 , C07C323/60 , C07F9/09 , C07F9/655 , C07H19/067 , C07H19/073 , C07H19/167 , C07H19/173 , C07K1/113 , C07K1/13 , C07K14/00 , C07K14/575 , C07K14/76 , C07K14/765 , C07K16/00 , C07K16/26 , C08B37/02 , C08G85/00 , G01N33/532 , G01N33/533 , G01N33/534 , G01N33/553 , G01N33/58 , C12Q1/68 , C08G77/04
CPC分类号： G01N33/534 , A61K51/0497 , A61K51/1093 , G01N33/533 , G01N33/553
摘要： A detectable molecule of the formulaA.sup.3 --(--X--R.sup.1 --E--Det.sup.b).sub.mwhere A.sup.3 is A.sup.2 or a polymer, where A.sup.3 has at least one modifiable reactive group selected from the group consisting of amino, hydroxy, cis OH, halides, aryl, imidazoyl, carbonyl, carboxy, thiol or a residue comprising an activated carbon; --X-- is selected from the group consisting of ##STR1## --R.sup.1 -- is ##STR2## or a C.sub.1 -C.sub.10 branched or unbranched alkyl or aralkyl, which may be substituted by --OH; --Y-- is a direct bond to --E--, or --Y-- is --E--R.sup.2 -- where R.sup.2 is a C.sub.1 -C.sub.10 branched or unbranched alkyl; Z.sub.a is chlorine, bromine or iodine; E is O, NH or an acyclic divalent sulfur atom; Det.sup.b is a chemical moiety capable of being detected, preferably comprising biotin or a metal chelator of the formula: ##STR3## or the 4-hydroxy or acyloxy derivative thereof, where R.sup.3 is C.sub.1 -C.sub.4 alkyl or CH.sub.2 COOM, M is the same or different and selected from the group consisting of hydrogen, a metal or non-metal cation or is C.sub.1 -C.sub.10 alkyl, aryl or aralkyl; and m is an integer from 1 to the total number of modified reactive groups on A.sup.3. The detectable molecules are useful in in virto or in vivo assays or therapy.
摘要翻译：式A3-（ - X-R1-E-Detb）m的可检测分子，其中A3是A2或聚合物，其中A3具有选自氨基，羟基，顺式OH，卤化物中的至少一个可修饰的反应性基团，芳基，咪唑基，羰基，羧基，硫醇或包含活性炭的残基; -X-选自 + TR OCONH -R1-是或C1-C10支链或非支链烷基或芳烷基，其可以被 - 哦; -Y-是与-E-或-Y-是-E-R 2 - 的直接键合，其中R 2是C 1 -C 10支链或非支链烷基; Za是氯，溴或碘; E是O，NH或无环二价硫原子; Detb是能够被检测的化学部分，优选包含下式的生物素或金属螯合剂：其中R 3是C 1 -C 4烷基或CH 2 COOM，M相同或不同并且选自氢，金属或非金属阳离子或是C 1 -C 10烷基，芳基或芳烷基; 并且m是从1到A3上修饰的反应性基团的总数的整数。可检测的分子可用于病毒或体内测定或治疗。

46. 发明授权

US09487821B2 Composition comprising library of double stranded nucleic acids 有权
公开(公告)号：US09487821B2
公开(公告)日：2016-11-08
申请号：US10902586
申请日：2004-07-29
申请人： Elazar Rabbani , Jannis G. Stavrianopoulos , James J. Donegan , Jack Coleman
发明人： Elazar Rabbani , Jannis G. Stavrianopoulos , James J. Donegan , Jack Coleman
IPC分类号： C12Q1/68 , C07H21/00 , C12N15/10 , B01J19/00 , C40B40/00
CPC分类号： C40B40/06 , B01J19/0046 , B01J2219/00599 , B01J2219/0061 , B01J2219/00612 , B01J2219/00648 , B01J2219/00722 , B01J2219/00725 , C07B2200/11 , C07H21/00 , C12N15/1006 , C12N15/1058 , C12Q1/68 , C12Q1/6809 , C12Q1/6825 , C12Q1/6837 , C40B40/00 , C40B40/10 , G01N33/68 , C12Q2565/501 , C12Q2563/179 , C12Q2545/114 , C12Q2525/155 , C12Q2565/514 , C12Q2565/537
摘要： This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.

47. 发明授权

US09316587B2 Processes for quantitative or qualitative detection of single-stranded or double-stranded nucleic acids 有权
公开(公告)号：US09316587B2
公开(公告)日：2016-04-19
申请号：US11235516
申请日：2005-09-26
申请人： Elazar Rabbani , Jannis G. Stavrianopoulos , James J. Donegan , Jack Coleman , Dakai Liu
发明人： Elazar Rabbani , Jannis G. Stavrianopoulos , James J. Donegan , Jack Coleman , Dakai Liu
IPC分类号： C12Q1/68 , G01N21/64
CPC分类号： C12Q1/6818 , C12Q1/6834 , C12Q1/6844 , C12Q1/6853 , C12Q1/686 , G01N21/64 , G01N21/6486 , C12Q2565/101 , C12Q2533/101 , C12Q2561/113
摘要： This invention provides for compositions for use in real time nucleic acid detection processes. Such real time nucleic acid detection processes are carried out with energy transfer elements attached to nucleic acid primers, nucleotides, nucleic acid probes or nucleic acid binding agents. Real time nucleic acid detection allows for the qualitative or quantitative detection or determination of single-stranded or double-stranded nucleic acids of interest in a sample. Other processes are provided by this invention including processes for removing a portion of a homopolymeric sequence, e.g., poly A sequence or tail, from an analyte or library of analytes. Compositions useful in carrying out such removal processes are also described and provided.

48. 发明授权

US09309563B2 Compositions and processes for analyte detection, quantification and amplification 有权
标题翻译：用于分析物检测，定量和扩增的组合物和方法
公开(公告)号：US09309563B2
公开(公告)日：2016-04-12
申请号：US10900453
申请日：2004-07-27
申请人： Elazar Rabbani , Jannis G. Stavrianopoulos , James J. Donegan , Jack Coleman
发明人： Elazar Rabbani , Jannis G. Stavrianopoulos , James J. Donegan , Jack Coleman
IPC分类号： C12P19/34 , C12Q1/68 , C07H21/00 , C12N15/10 , C40B40/00
CPC分类号： C40B40/06 , B01J19/0046 , B01J2219/00599 , B01J2219/0061 , B01J2219/00612 , B01J2219/00648 , B01J2219/00722 , B01J2219/00725 , C07B2200/11 , C07H21/00 , C12N15/1006 , C12N15/1058 , C12Q1/68 , C12Q1/6809 , C12Q1/6825 , C12Q1/6837 , C40B40/00 , C40B40/10 , G01N33/68 , C12Q2565/501 , C12Q2563/179 , C12Q2545/114 , C12Q2525/155 , C12Q2565/514 , C12Q2565/537
摘要： This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.
摘要翻译：本发明提供了用于分析物检测，定量和扩增的新型组合物和方法。分析物的核酸阵列和文库可有效地并入到这样的组合物和方法中。使用通用检测元件，信号实体等来检测并且如果需要或期望量化分析物。靶分析物的扩增也由本发明的组合物和方法提供。

49. 发明授权

US08846893B2 Non-ionically bound constructs, and compositions and kits comprising domains 失效
标题翻译：非离子结合构建体，以及包含结构域的组合物和试剂盒
公开(公告)号：US08846893B2
公开(公告)日：2014-09-30
申请号：US08978633
申请日：1997-11-25
申请人： Elazar Rabbani , Jannis G. Stavrianopoulos , James J. Donegan , Dakai Liu , Norman E. Kelker , Dean L. Engelhardt
发明人： Elazar Rabbani , Jannis G. Stavrianopoulos , James J. Donegan , Dakai Liu , Norman E. Kelker , Dean L. Engelhardt
IPC分类号： C07H21/04 , C12N15/113 , C12Q1/68 , C12N9/12 , C12Q1/70 , C07K14/005 , C07K14/62 , C12N15/87
CPC分类号： C12N15/1132 , C07K14/005 , C07K14/62 , C12N9/1247 , C12N15/87 , C12N2740/16322 , C12Q1/6897 , C12Q1/703 , C12Q2525/301 , C12Q2525/307
摘要： The present invention provides an array of compositions useful for effecting and/or exhibiting changes in biological functioning and processing within cells and in biological systems containing such cells. In effect, these compositions combine chemical modifications and/or ligand additions with biological functions. The chemical modifications and/or ligand additions provide additional characteristics to the compositions without interfering substantially with their biological function. Such additional characteristics include nuclease resistance, targeting specific cells or specific cell receptors localizing to specific sites within cells and augmenting interactions between the compositions and target cells of interest as well as decreasing such interactions when desired. Also provided by the present invention are processes and kits.
摘要翻译：本发明提供了一组组合物，其用于在细胞内和含有这种细胞的生物系统中实现和/或表现出生物功能和加工中的变化。实际上，这些组合物将化学修饰和/或配体添加与生物学功能相结合。化学修饰和/或配体添加为组合物提供额外的特征，而不会基本上干扰它们的生物学功能。这样的附加特征包括核酸酶抗性，靶向特定细胞或定位于细胞内特定位点的特定细胞受体，并且增加组合物和靶细胞之间的相互作用，并且当需要时减少这些相互作用。本发明还提供了方法和试剂盒。

50. 发明授权

US08486633B2 Method for detecting nucleic acid sequences 有权
标题翻译：检测核酸序列的方法
公开(公告)号：US08486633B2
公开(公告)日：2013-07-16
申请号：US11925730
申请日：2007-10-26
申请人： Elazar Rabbani , Jannis G. Stavrianopoulos , James J. Donegan , Jack Coleman , Marleen Walner
发明人： Elazar Rabbani , Jannis G. Stavrianopoulos , James J. Donegan , Jack Coleman , Marleen Walner
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6869 , C12P19/34 , C12Q1/6844 , C12Q1/6853 , C12Q2531/119 , C12Q2525/301 , C12Q2525/161
摘要： The invention is directed to a method for detecting a target nucleic acid sequence by non-linear amplification.
摘要翻译：本发明涉及通过非线性扩增检测靶核酸序列的方法。

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