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41. 发明专利

JP2005185101A VEGETABLE FULL-LENGTH cDNA AND UTILIZATION THEREOF 审中-公开
标题翻译：蔬菜全长cDNA及其利用
公开(公告)号：JP2005185101A
公开(公告)日：2005-07-14
申请号：JP2002383870
申请日：2002-12-11
申请人： Bio Oriented Technol Res Advancement Inst , Foundation For Advancement Of International Science , Institute Of Physical & Chemical Research , National Institute Of Agrobiological Sciences , 独立行政法人理化学研究所 , 独立行政法人農業生物資源研究所 , 生物系特定産業技術研究推進機構 , 財団法人国際科学振興財団
发明人： KIKUCHI HISASHI , KISHIMOTO NAOKI , SATO KOJI , NAGATA TOSHIBUMI , KAWAZU NOBUYUKI , YAZAKI JIYUNJI , ISHIKAWA MASAHIRO , DOI KOUJI , KAWAI JUN , HAYASHIZAKI YOSHIHIDE , OTOMO YASUHIRO , MATSUBARA KENICHI , MURAKAMI KAZUO
IPC分类号： A01H5/00 , C07K14/415 , C07K16/16 , C12N5/10 , C12N9/00 , C12N9/02 , C12N9/12 , C12N9/14 , C12N9/26 , C12N9/32 , C12N9/42 , C12N15/09 , C12P21/02 , C12Q1/68
CPC分类号： C07K14/415
摘要： PROBLEM TO BE SOLVED: To provide a vegetable complete-length cDNA and uses thereof. SOLUTION: Rice full-length cDNAs of 28,694 clones are isolated. Among them, 21,596 clones (75.86%) are annotated through homology searches. The homology is held by 18,900 clones (64%) of genes (27,288 clones) predicted from a genomic sequence of Arabidopsis thaliana. The full-length cDNA clones fulfill an important role in annotation or determination of exon and intron in a correct gene coding region or comprehensive expression analysis or proteome analysis on the transcription level. The full-length cDNAs have industrial usefulness such as creation of plant bodies having characters different from those of the wild type by expression or suppression of functions in the plant bodies. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：待解决的问题：提供一种植物性全长cDNA及其用途。解决方案：分离28,694个克隆的水稻全长cDNA。其中通过同源性搜索注释了21,596个克隆（75.86％）。同源性由拟南芥拟南芥基因组序列预测的18,900个克隆（64％）基因（27,288个克隆）进行。全长cDNA克隆在正确的基因编码区域的外显子和内含子的注释或测定中起着重要作用，或者在转录水平上进行综合表达分析或蛋白质组学分析。全长cDNA具有工业用途，例如通过表达或抑制植物体内的功能来产生具有与野生型不同的特征的植物体。版权所有（C）2005，JPO＆NCIPI

42. 发明专利

JP2005168472A Promotor having leaf specific expression activity 有权
标题翻译：具有叶特异性表达活性的促销者
公开(公告)号：JP2005168472A
公开(公告)日：2005-06-30
申请号：JP2003416961
申请日：2003-12-15
申请人： National Institute Of Agrobiological Sciences , 独立行政法人農業生物資源研究所
发明人： ICHIKAWA HIROAKI , TANAKA HIROSHI , NAKAMURA EIKO , SASAKI TAKUJI , KIKUCHI HISASHI
IPC分类号： A01H5/00 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a DNA having a promotor activity bringing a leaf specific expression, a recombinant plasmid containing the DNA and enabling the expression of an objective gene as leaf specific, and a transformed plant body introduced with the recombinant vector. SOLUTION: This DNA can function as a promotor shown in the following (a), (b) or (c). (a) A DNA consists of a specific base sequence derived from rice, (b) A DNA consists of a base sequence obtained by deleting, substituting or adding one or several bases in the DNA derived from the rice and having the promotor activity for bringing the leaf specific expression. (c) A DNA consists of the part of a specific base sequence derived from the rice and having the promotor activity for bringing the leaf specific expression. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：待解决的问题：为了提供具有叶特异性表达的启动子活性的DNA，含有该DNA的重组质粒，能够将目的基因表达为叶特异性，以及引入重组载体的转化植物体。解决方案：该DNA可用作以下（a），（b）或（c）所示的启动子。（a）DNA由来自水稻的特异性碱基序列组成，（b）DNA由通过缺失，取代或添加一种或几种碱基而获得的碱基序列，所述DNA来源于所述水稻，并具有启动子活性，叶特异性表达。（c）DNA由来自水稻的特定碱基序列的一部分组成，并具有用于使叶特异性表达的启动子活性。版权所有（C）2005，JPO＆NCIPI

43. 发明专利

JP2005110594A Dna fragment for expressing virus vector and utilization thereof 有权
标题翻译：用于表达病毒载体的DNA片段及其利用
公开(公告)号：JP2005110594A
公开(公告)日：2005-04-28
申请号：JP2003350091
申请日：2003-10-08
申请人： Japan Science & Technology Agency , National Institute Of Agrobiological Sciences , 独立行政法人科学技術振興機構 , 独立行政法人農業生物資源研究所
发明人： MORI MASAYUKI , DOI KOJI , NISHIGORI MASAKI , HAN TETSUO , ISHIKAWA MASAYUKI
IPC分类号： C12N15/09 , C12N5/10 , C12P21/02
摘要： PROBLEM TO BE SOLVED: To provide a DNA fragment usable for enabling a plant to produce an arbitrary useful protein, and to provide a method for utilizing the DNA fragment. SOLUTION: The accumulation of a virus RNA can be increased considerably by the cleavage of an excess sequence added to the 3' terminus of the virus RNA transcribed in a cell from a cDNA by a ribozyme by introducing a DNA fragment obtained by linking a ribozyme sequence to the cDNA of the virus vector into which a gene encoding the arbitrary useful protein is inserted, to the cell. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：待解决的问题：提供可用于使植物产生任意有用蛋白质的DNA片段，并提供利用该DNA片段的方法。解决方案：通过引入通过连接获得的DNA片段，通过核酶从cDNA转录到细胞中的病毒RNA的3'末端的多余序列的切割，可以显着增加病毒RNA的积累将编码任意有用蛋白质的基因的病毒载体的cDNA的核酶序列插入细胞。版权所有（C）2005，JPO＆NCIPI

44. 发明专利

JP2005110579A PHOTOSENSITIVE GENE Hd5 FOR PLANT AND USE OF THE GENE 有权
标题翻译：用于植物和基因使用的光敏基因Hd5
公开(公告)号：JP2005110579A
公开(公告)日：2005-04-28
申请号：JP2003349338
申请日：2003-10-08
申请人： National Institute Of Agrobiological Sciences , Society For Techno-Innovation Of Agriculture Forestry & Fisheries , 独立行政法人農業生物資源研究所 , 社団法人農林水産先端技術産業振興センター
发明人： YANO MASAHIRO , YAMAUCHI UTAKO
IPC分类号： A01H5/00 , A01N63/00 , C07K14/415 , C07K16/16 , C12N5/10 , C12N15/09 , C12P21/02 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To provide a new photosensitive gene for plant, to provide a method for modifying plant photosensitivity using the gene, and to provide a method for modifying plant heading date. SOLUTION: The photosensitive gene Hd5 for Oryza sativa is successfully isolated by map base cloning. It is found that plant photosensitivity can be modified by transferring the gene or controlling its expression. Furthermore, it is found that plant photosensitivity can be evaluated by detecting the presence/absence of the functional gene. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：为植物提供新的感光基因，提供使用该基因修饰植物光敏性的方法，并提供修改植物标题日期的方法。解决方案：通过地图碱克隆成功分离了水稻的光敏基因Hd5。发现植物光敏性可以通过转移基因或控制其表达来修饰。此外，发现可以通过检测功能基因的存在/不存在来评价植物光敏性。版权所有（C）2005，JPO＆NCIPI

45. 发明专利

JP2005073533A Aequorin and method for purifying and separating the same 有权
标题翻译： AEQUORIN和用于净化和分离其的方法
公开(公告)号：JP2005073533A
公开(公告)日：2005-03-24
申请号：JP2003305743
申请日：2003-08-29
申请人： National Institute Of Agrobiological Sciences , Nec Soft Ltd , Necソフト株式会社 , 独立行政法人農業生物資源研究所
发明人： OTSUKA MOTOHISA , MIZUNO HIROSHI , TSUJI FREDERIC ICHIRO , TAKENAKA HIROMI
IPC分类号： G01N33/50 , B01D15/08 , C07K14/435 , C12N9/02 , C12Q1/26 , G01N21/78 , G01N30/34 , G01N30/88
CPC分类号： C12N9/0069 , C07K14/43595
摘要： PROBLEM TO BE SOLVED: To use highly pure aequorin reproduced from recombinant apoaequorin, thereby enhancing detection accuracy on the measurement of the concentration of calcium ion. SOLUTION: In a solution of aequorin reproduced from recombinant apoaequorin, a concentration gradient elution chromatography method is used to remove the isoforms of the apoaequorin and non-luminous isoforms contained in the aequorin-reproduced solution. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：使用从重组载脂蛋白重现的高纯度水母发光蛋白，从而提高钙离子浓度测量的检测精度。解决方案：在重组载脂蛋白复制的水母发光蛋白溶液中，使用浓度梯度洗脱色谱法去除水母发光蛋白再生溶液中所含的脱辅基调蛋白和非发光同种型的同种型。版权所有（C）2005，JPO＆NCIPI

46. 发明专利

JP2005000167A Method for detection of methylation of genome 有权
标题翻译：检测基因组甲基化的方法
公开(公告)号：JP2005000167A
公开(公告)日：2005-01-06
申请号：JP2004150574
申请日：2004-05-20
申请人： National Institute Of Agrobiological Sciences , 独立行政法人農業生物資源研究所
发明人： OKUIZUMI HISATO
IPC分类号： G01N33/50 , C12N15/09 , C12Q1/34 , C12Q1/68 , G01N27/447
摘要： PROBLEM TO BE SOLVED: To provide a method for effectively and inexpensively detecting methylation of a genome DNA. SOLUTION: In a RGLS method (Restriction enzyme Landmark Genome Scanning method), various detection of methylation of genome is carried out by cutting DNA of genome with a restriction enzyme which is a restriction enzyme recognizing 4 base group or 5 base group in a secondary restriction enzyme treatment before one-dimensional electrophoresis and a DNA digestion reaction of the enzyme is inhibited by methylation of the bases of the recognizing sequence and/or adjacent sequence. The effective and inexpensive detection of methylation of genome can be carried out by using a frequently cutting methylation sensitivity restricting enzyme. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供一种有效且廉价地检测基因组DNA甲基化的方法。解决方案：在RGLS方法（限制性酶标记基因组扫描方法）中，通过用限制酶切割基因组的DNA来进行基因组的甲基化的各种检测，限制酶是识别4个碱基或5个碱基的限制酶在一维电泳和酶的DNA消化反应之前的第二限制酶处理被识别序列和/或相邻序列的碱基的甲基化抑制。可以通过使用频繁切割的甲基化敏感性限制酶来进行基因组甲基化的有效且廉价的检测。版权所有（C）2005，JPO＆NCIPI

47. 发明专利

JP2004321055A New genetic marker for spikelet indeciduous gene or the like and use thereof 有权
标题翻译： SPIKELET INDECIDUUUS基因的新遗传标记或其类似物及其用途
公开(公告)号：JP2004321055A
公开(公告)日：2004-11-18
申请号：JP2003119023
申请日：2003-04-23
申请人： Japan Science & Technology Agency , National Institute Of Agrobiological Sciences , 独立行政法人科学技術振興機構 , 独立行政法人農業生物資源研究所
发明人： TAKEDA KAZUYOSHI , KOMATSUDA TAKAO , NATESAN SENSHIRU , PETER MAXIM , MANO YOSHIRO , DHANASEKARAN VIDYA SARASWATHI , AZHAGUVEL PERUMAL
IPC分类号： C12N15/09 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To obtain a new genetic marker, especially a new genetic marker positioned in the vicinity of spikelet indeciduous gene btr1 and btr2 in barley 3HS chromosome and to provide a use thereof. SOLUTION: The genetic marker which is obtained by AFLP (R) procedure of barley genome DNA lies at any position selected from the same position as those on the chromosomes of spikelet indeciduous gene btr1 and btr2 of barley, positions at 0.5cM, 1.0cM and 2.0cM telomere side from the positions on the chromosomes of the btr1 and the btr2, respectively and positions at 2.7cM, 3.2cM, 3.7cM, 4.2cM and 4.7cM centromere side from the positions on the chromosomes of the brt1 and the btr2, respectively. The genetic marker is useful for isolating and/or identifying the spikelet indeciduous gene btr1 and btr2. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：为了获得新的遗传标记，特别是位于大麦3HS染色体中的小穗独特基因btr1和btr2附近的新遗传标记，并提供其用途。解决方案：通过大麦基因组DNA的AFLP（R）程序获得的遗传标记位于选自与小麦的小穗杂种基因btr1和btr2的染色体上相同位置的任何位置，位置为0.5cM，分别从btr1和btr2的染色体上的位置分别为1.0cM和2.0cM端粒侧，位置分别为从bt1和btr2的染色体上的位置处的2.7cM，3.2cM，3.7cM，4.2cM和4.7cM着丝粒的位置; btr2。遗传标记对于分离和/或鉴定小穗独特基因btr1和btr2是有用的。版权所有（C）2005，JPO＆NCIPI

48. 发明专利

JP2004313184A Method for selecting undifferentiated cell and use of the same 有权
标题翻译：选择不同细胞的方法及其用途
公开(公告)号：JP2004313184A
公开(公告)日：2004-11-11
申请号：JP2004093665
申请日：2004-03-26
申请人： National Agriculture & Bio-Oriented Research Organization , National Institute Of Agrobiological Sciences , 独立行政法人農業・生物系特定産業技術研究機構 , 独立行政法人農業生物資源研究所
发明人： TOKUNAGA TOMOYUKI , FURUSAWA KI , HONDA KURIO , OGOSHI KATSUHIRO
IPC分类号： A01K67/027 , C12N5/07 , C12N5/0735 , C12Q1/02 , G01N33/48 , C12N5/06
摘要： PROBLEM TO BE SOLVED: To easily obtain a germ line chimera, by increasing production efficiency, when a chimera individual is produced by using an undifferentiated cell, and increasing a contribution ratio of the undifferentiated cell in the chimera individual. SOLUTION: A method for selecting the undifferentiated cell comprises conducting selection of an undifferentiated cell subgroup by using a cell surface marker as an indicator, so that a cell group which forms the chimera in a high yield is preparatively isolated, wherein the method is developed based on such a discovery that cell subgroups the characteristics of which are fractionized from each other by the cell surface marker existing in a single embryonic stem (ES) cell strain, and a difference in ratios of chimera formation by the ES cell strain is not dependent on characteristics of the cell strain itself, but is influenced by a variation in compositions of the subgroups contained therein. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：待解决的问题：通过提高生产效率，当通过使用未分化细胞产生嵌合体个体并增加嵌合体个体中未分化细胞的贡献比时，容易获得种系嵌合体。解决方案：选择未分化细胞的方法包括通过使用细胞表面标记作为指标来进行未分化细胞亚组的选择，从而制备性分离形成高产量的嵌合体的细胞组，其中所述方法是基于这样的发现开发的，其中细胞亚组的特征通过存在于单个胚胎干（ES）细胞株中的细胞表面标记物相互分离，并且ES细胞株的嵌合体形成比率差异为不依赖于细胞株本身的特征，而是受其中所含亚组的组成变化的影响。版权所有（C）2005，JPO＆NCIPI

49. 发明专利

JP2004295654A Method for estimating function of protein by use of ligand library 审中-公开
标题翻译：通过使用LIGAND图书馆估计蛋白质功能的方法
公开(公告)号：JP2004295654A
公开(公告)日：2004-10-21
申请号：JP2003088900
申请日：2003-03-27
申请人： National Institute Of Agrobiological Sciences , 独立行政法人農業生物資源研究所
发明人： MAEDA YOSHINORI
IPC分类号： G06F17/30
摘要： PROBLEM TO BE SOLVED: To estimate the in vivo function of a protein with unknown function based on a three-dimensional structure determined by a structural biological experimental method or estimated by modeling. SOLUTION: The in vivo function of the protein is estimated by use of a virtual ligand library (database) in a biomolecule. COPYRIGHT: (C)2005,JPO&NCIPI

50. 实用新型

JP3104661U Food sampler 失效
公开(公告)号：JP3104661U
公开(公告)日：2004-10-07
申请号：JP2004002088
申请日：2004-04-15
申请人：独立行政法人農業生物資源研究所National Institute Of Agrobiological Sciences
发明人：忠由三橋
IPC分类号： G01N1/04 , G01N1/00
摘要：【課題】食肉試料よりDNAを抽出し検査に供する場合、他の試料及びヒトの手からの汚染を完全に防ぐため、対象に直接手を触れないように試料ごとに手術用の手袋を着交換するか徹底的な洗浄を行い試料採取を行うことが必要とされ、試料採取に非常な労力が伴っている。
【解決手段】食肉採取具と保管具を一体化させ、片手で食肉試料を採取可能とし、食肉試料に触れることなく、食肉試料を保管できる器具の開発・提供した。
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