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31. 发明授权

US4071410A Process for preparation of pancreatic elastase 失效
标题翻译：胰弹性蛋白酶的制备方法
公开(公告)号：US4071410A
公开(公告)日：1978-01-31
申请号：US738866
申请日：1976-11-04
申请人： Masayuki Yoshizawa
发明人： Masayuki Yoshizawa
IPC分类号： C12N9/66 , C07G7/026
CPC分类号： C12N9/6448
摘要： Pancreatic elastase is prepared by adding the duodenum of a mammalian animal or an extract thereof to a starting material containing a precursor of elastase to thereby activate the elastase precursor and separating the obtained pancreatic elastase.
摘要翻译：通过将哺乳动物的十二指肠或其提取物添加到含有弹性蛋白酶前体的原料中来制备胰腺弹性蛋白酶，从而活化弹性蛋白酶前体并分离所获得的胰弹性蛋白酶。

32. 发明授权

US4070242A Method for the preparation of prostaglandin synthetase 失效
标题翻译：前列腺素合成酶的制备方法
公开(公告)号：US4070242A
公开(公告)日：1978-01-24
申请号：US780258
申请日：1977-03-23
申请人： Sheikh Arshad Saeed
发明人： Sheikh Arshad Saeed
IPC分类号： A61K38/53 , C07G7/026
CPC分类号： A61K38/53
摘要： A method for the preparation of purified prostaglandin synthetase from bovine seminal vesicles is disclosed. The method includes the steps of homogenizing bovine seminal vesicles with a liquid medium to form an homogenate and centrifuging the homogenate at a speed such that the centrifugal force does not exceed 1,000g. The supernatant obtained, containing prostaglandin synthetase, is separated from the solids.
摘要翻译：公开了从牛精囊制备纯化的前列腺素合成酶的方法。该方法包括以下步骤：用液体培养基均匀化牛精液囊泡以形成匀浆并以一定的速度离心匀浆物，使得离心力不超过1,000g。将得到的含有前列腺素合成酶的上清液与固体分离。

33. 发明授权

US4035234A Process for the preparation of the kallikrein-trypsin inhibitor 失效
标题翻译：制备激肽释放酶 - 胰蛋白酶抑制剂的方法
公开(公告)号：US4035234A
公开(公告)日：1977-07-12
申请号：US661160
申请日：1976-02-25
申请人： Hermann Schutt
发明人： Hermann Schutt
IPC分类号： A61K35/42 , A61K38/55 , C07K14/81 , C07G7/026
CPC分类号： C07K14/8117 , Y10S435/832 , Y10S435/842 , Y10S435/913
摘要： The kallikrein-trypsin inhibitor is obtained from animal organs by proteolytic digestion of the organ at an elevated temperature and recovery of kallikrein-trypsin inhibitor by selective adsorption thereof on carrier-bound trypsin and subsequent desorption.
摘要翻译：激肽释放酶 - 胰蛋白酶抑制剂通过在升高的温度下对器官进行蛋白水解消化从动物器官获得，并通过选择性吸附载体结合的胰蛋白酶并随后解吸回收激肽释放酶 - 胰蛋白酶抑制剂。

34. 发明授权

US4028187A Method of obtaining urokinase 失效
标题翻译：获得尿激酶的方法
公开(公告)号：US4028187A
公开(公告)日：1977-06-07
申请号：US701930
申请日：1976-07-01
申请人： Abe Tsutomu , Hashino Yasuo , Kobayashi Hidehiko
发明人： Abe Tsutomu , Hashino Yasuo , Kobayashi Hidehiko
IPC分类号： C12N9/72 , C07G7/026
CPC分类号： C12N9/6462 , C12Y304/21073 , Y10S435/815
摘要： A method of obtaining urokinase directly from human urine comprising contacting human urine with an acrylonitrile polymer of a unique porous structure formed at the time of polymerization for formation of the polymer and having a porosity of not less than 10% and a specific surface area of 5 m.sup.2 /g. and subsequently eluting the urokinase adsorbed on the polymer. The acrylonitrile polymer employed in the method of the present invention has an excellent adsorption activity to urokinase and therefore, with use of an extremely small amount of the adsorbent, the satisfactory results can be obtained. Moreover, the porous polymer of the present method can also be utilized for purification of low purity crude urokinase obtained by the prior art methods.
摘要翻译：一种从人尿中直接获得尿激酶的方法，包括使人尿与聚合时形成的独特多孔结构的丙烯腈聚合物接触形成聚合物，孔隙率不小于10％，比表面积为5 m2 / g。随后洗脱吸附在聚合物上的尿激酶。本发明方法中使用的丙烯腈聚合物对尿激酶具有优异的吸附活性，因此，通过使用极少量的吸附剂，可以获得令人满意的结果。此外，本方法的多孔聚合物也可用于纯化通过现有技术方法获得的低纯度尿尿激酶。

35. 发明授权

US3986927A Process for the purification and sterilization of acidophilic biologicals by extreme acidification at cold temperatures 失效
标题翻译：通过极端酸化在寒冷温度下净化和灭菌的方法
公开(公告)号：US3986927A
公开(公告)日：1976-10-19
申请号：US572565
申请日：1975-04-28
申请人： Joseph L. Melnick , Craig Wallis
发明人： Joseph L. Melnick , Craig Wallis
IPC分类号： A61K38/48 , A61L2/02 , C07G7/02 , C07G7/026
CPC分类号： A61K38/4826 , A61L2/02
摘要： Methods of sterilizing biologicals in which the biologicals are acidified to a pH of not higher than about 1.0, preferably 0.75 to about 0.5, and the acidified biologicals are stored at cold temperatures for a perod of time sufficient to inactivate microbial flora contained therein but not the biological itself, for example, in the range of about 0.degree. to about 10.degree. C for 1-24 hours or longer. Precipitates formed during the storing are clarified and the biologicals are then neutralized, preferably to a pH of from about 7.0 to about 8.0, or that pH at which the biological is best stored, e.g., trypsin at pH 3-4. The methods include clarifying biologicals of precipitates formed within the first two to three hours and then continuing the storing and again clarifying the biologicals of precipitates.Microbial flora and numerous contaminated proteins contained in the biologicals are removed resulting in completely sterilized biologicals retaining initial activity or titer. If desired, the clarified biologicals may be stored in acidic state since advantageously they reman biologically stable at ambient temperatures when stored at low pH levels, and then neutralized shortly before dispensing. A number of examples is set forth.

36. 发明授权

US3943245A Purification of plasminogen 失效
标题翻译：纤溶酶原的纯化
公开(公告)号：US3943245A
公开(公告)日：1976-03-09
申请号：US440206
申请日：1974-02-14
申请人： Robert M. Silverstein
发明人： Robert M. Silverstein
IPC分类号： C12N9/68 , A61K35/14 , A61K35/16 , C07G7/00 , C07G7/026
CPC分类号： C12N9/6435 , C12Y304/21007 , Y10S530/83 , Y10S530/831
摘要： The purification of plasminogen from human and non-human mammalian plasma or Cohn Fraction III by modified affinity chromotography using Sepharose-L-lysine with high ionic strength buffer solutions as in ion exchange chromatography is described. A plasminogen is produced in great yield having unexpectedly enhanced activity toward N.sup..alpha.-CBZ-L-lysine p-nitrophenyl ester, enhanced fibrinolytic activity and significantly less contamination by unknown colloidal substances.
摘要翻译：描述了通过使用Sepharose-L-赖氨酸与离子交换层析中的高离子强度缓冲溶液的改进的亲和层析法从人和非人哺乳动物血浆或Cohn Fraction III中纯化纤溶酶原。以大量的产率产生纤溶酶原，意外地增强了对N-CZZ-L-赖氨酸对硝基苯酯的活性，增强的纤维蛋白溶解活性和明显较少的未知胶体物质的污染。

37. 发明授权

US3905870A Purification of kallikrein 失效
标题翻译：激肽释放酶的纯化
公开(公告)号：US3905870A
公开(公告)日：1975-09-16
申请号：US43154673
申请日：1973-12-26
申请人： BAYER AG
发明人： KUTZBACH CARL , SCHMIDT-KASTNER GUNTHER
IPC分类号： C12N9/64 , A61K19/00 , C07G7/026
CPC分类号： C12N9/6445 , Y10S435/815 , Y10S435/816
摘要： There is disclosed a process for the purification of Kallikrein, comprising components, Kallikrein A and Kallikrein B, otherwise known as the enzyme, Kininogenase, which includes the process steps of: 1. TREATING A CRUDE AQUEOUS SOLUTION CONTAINING Kallikrein with a solution of a lead or zinc salt to produce a precipitate containing Kallikrein; 2. ELUTING Kallikrein from said precipitate to produce a solution of Kallikrein; 3. DESALINATING SAID SOLUTION OF Kallikrein to produce a desalinated solution of Kallikrein; 4. ADSORBING THE Kallikrein from the desalinated solution onto a macroporous anion exchange resin and eluting it therefrom to obtain an eluted solution containing Kallikrein; and thereupon 5. CONTACTING THE ELUTED SOLUTION WITH A MACROPOROUS CATION EXCHANGER; AND SEPARATING SAID CATION EXCHANGER TO PRODUCE A PURE SOLUTION OF Kallikrein. The disclosed process also includes means for the production of purified components, Kallikrein A and Kallikrein B, as well as the crystallization of Kallikrein and its components and to pharmaceuticals and medicaments containing the same.
摘要翻译：公开了一种用于纯化激肽释放酶的方法，其包括组分，激肽释放酶A和激肽释放酶B，还称为酶，激肽原酶，其包括以下步骤：

38. 发明授权

US3904480A Enhanced production of plasminogen activator 失效
标题翻译：增强纤溶酶原激活物的产生
公开(公告)号：US3904480A
公开(公告)日：1975-09-09
申请号：US48548974
申请日：1974-07-03
申请人： LILLY CO ELI
发明人： HULL ROBERT N , HUSEBY ROLF M
IPC分类号： C12N9/72 , C07G7/026
CPC分类号： C12N9/6456 , Y10S435/815
摘要： Enhanced plasminogen activator yields are obtained by culturing plasminogen activator producing mammalian tissue cells in an aqueous nutrient culture medium until cells have attained development to confluency or maximum population density; continuing incubation in the presence of an antimitotic agent, e.g., podophylotoxin, colchicine and derivatives thereof, vincristine, vinblastine and derivatives thereof; recovering the activator from the separated aqueous culture medium by adsorption on hydroxyapatite and elution therefrom; and purifying the activator via salt precipitation followed by DEAE cellulose chromatography and gel filtration chromatography. Plasminogen activator is provided in clinically useful amounts for treatment of thromboembolic conditions.
摘要翻译：通过在水性营养培养基中培养产生哺乳动物组织细胞的纤溶酶原激活剂获得增强的纤溶酶原激活物产量，直到细胞达到融合或最大群体密度; 在抗有丝分裂剂，例如足细菌毒素，秋水仙素及其衍生物，长春新碱，长春花碱及其衍生物的存在下继续孵育; 通过在羟基磷灰石上吸附并从其中洗脱从分离的水培养基中回收活化剂; 并通过盐沉淀，然后用DEAE纤维素层析和凝胶过滤层析纯化活化剂。提供临床有用量的纤溶酶原激活剂用于治疗血栓栓塞症状。

39. 发明授权

US3723251A Method for extracting urokinase 失效
标题翻译：提取尿激酶的方法
公开(公告)号：US3723251A
公开(公告)日：1973-03-27
申请号：US3723251D
申请日：1971-08-23
申请人： MOCHIDA PHARM CO LTD
发明人： MURAYAMA Y , OGAWA N , KOJI N
IPC分类号： A61K35/22 , C12N9/72 , D01F6/18 , C07G7/026
CPC分类号： C12N9/6462 , C12Y304/21073 , Y10S435/815
摘要： ACRYLONITRILE MATERIAL IS USED TO ADSORB UROKINASE FROM A LIQUID, AND SAID UROKINASE IS SUBSEQUENTLY ELUTED FROM SAID MATERIAL.

40. 发明授权

US3542646A Process for fractionally obtaining urokinase and blood coagulation accelerator in human urine 失效
$Process for fractionally obtaining urokinase and blood coagulation accelerator in human urine$
标题翻译：在人体尿液中快速获得尿激酶和血液凝固加速器的方法
公开(公告)号：US3542646A
公开(公告)日：1970-11-24
申请号：US3542646D
申请日：1967-11-15
申请人： GREEN CROSS CORP
发明人： AOKI NOBUO , ASADA TOSHIO
IPC分类号： C12N9/72 , C07G7/026
CPC分类号： C12N9/6462 , C12Y304/21073 , Y10S435/815

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