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    • 40. 发明公开
    • 베타 자일로시다아제를 발현하는 재조합 바실러스 균주 및그 제조방법
    • 表达β-XYLOSIDASE的重组杆菌菌株及其使用重组载体pKYXy12的制备方法
    • KR1020040108199A
    • 2004-12-23
    • KR1020030039100
    • 2003-06-17
    • (주)바이오솔루션
    • 백현동임성렬
    • C12N15/74
    • C12N15/75C12Y302/01032
    • PURPOSE: Provided are a recombinant Bacillus strain which expresses β-xylosidase and a preparation method thereof by using a vector pKYXy12. Therefore, β-xylosidase having improved temperature stability and extended pH stability is mass-produced at reduced cost. CONSTITUTION: The recombinant Bacillus strain, Bacillus polyfermenticus SCD pKYXy12(KCCM-10487), expresses β-xylosidase. The method for preparation of Bacillus polyfermenticus SCD pKYXy12(KCCM-10487) comprises the steps of: constructing a recombinant vector pKY1 by cloning a vector p8A1 into a Bacillus-subtilis-E. coli shuttle vector pRB373; constructing a recombinant vector pKYXy11 by cloning a plasmid pXy11120 containing a gene which encodes cyclodextrinase derived from Bifidobacterium adolescentis lnt 57 into the recombinant vector pKY1; treating the recombinant vector pKYXy11 with restriction enzymes SmaI and EcoRV to prepare a recombinant vector pKYXy12; and transforming Bacillus polyfermenticus with the recombinant vector pKYXy12.
    • 目的:提供通过使用载体pKYXy12表达β-木糖苷酶的重组芽孢杆菌菌株及其制备方法。 因此,以降低成本大量生产具有改善的温度稳定性和延长的pH稳定性的β-木糖苷酶。 构成:重组芽孢杆菌菌株(Bacillus polyfermenticus)SCD pKYXy12(KCCM-10487)表达β-木糖苷酶。 芽孢杆菌SCD pKYXy12(KCCM-10487)的制备方法包括以下步骤:通过将载体p8A1克隆到枯草芽孢杆菌-E中构建重组载体pKY1。 大肠杆菌穿梭载体pRB373; 通过将含有编码来自青春双歧杆菌Bnt 57的环糊精酶的基因的质粒pXy11120克隆到重组载体pKY1中来构建重组载体pKYXy11; 用限制酶SmaI和EcoRV处理重组载体pKYXy11以制备重组载体pKYXy12; 并用重组载体pKYXy12转化Bacillus polyfermenticus。