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31. 发明授权

US11999802B2 Compositions and methods for selective protein degradation 有权
公开(公告)号：US11999802B2
公开(公告)日：2024-06-04
申请号：US16757026
申请日：2018-10-18
申请人： Novartis AG
发明人： James E. Bradner , Andrei Golosov , Carleton Proctor Goold , Carla Patricia Pinto Guimaraes , Marc Horst Peter Hild , Gregory Motz , Nathan Thomas Ross , Jonathan M. Solomon , Rohan Eric John Beckwith , Seth Carbonneau
IPC分类号： C07K19/00 , A61K31/4545 , A61K31/46 , A61K35/12 , C07D209/48 , C07D213/81 , C07D401/14 , C07D451/02 , C07K14/435 , C07K14/705 , C07K14/725 , C07K16/18 , C07K16/28 , C12N9/06 , C12N15/63 , C12N15/86 , G01N33/68
CPC分类号： C07K19/00 , A61K31/4545 , A61K31/46 , A61K35/12 , C07D209/48 , C07D213/81 , C07D401/14 , C07D451/02 , C07K14/435 , C07K14/705 , C07K14/7051 , C07K14/70521 , C07K14/70575 , C07K16/18 , C07K16/2803 , C07K16/2815 , C07K16/2863 , C07K16/2875 , C07K16/2887 , C07K16/2896 , C12N9/003 , C12N15/63 , C12N15/86 , C12Y105/01003 , G01N33/6803 , C07K2317/622 , C07K2319/02 , C07K2319/03 , C07K2319/20 , C07K2319/35 , C07K2319/81 , C07K2319/95 , C12N2740/15043
摘要： The invention provides compositions including a fusion polypeptide and methods for making a fusion polypeptide that includes a COF1/CRBN-binding polypeptide, COF2/CRBN-binding polypeptide, or COF3/CRBN-binding polypeptide and a heterologous polypeptide of interest.

32. 发明公开

US20240011010A1 SYNTHETIC PROTEIN-LEVEL NEURAL NETWORK IN MAMMALIAN CELLS 审中-公开
公开(公告)号：US20240011010A1
公开(公告)日：2024-01-11
申请号：US18347423
申请日：2023-07-05
申请人： California Institute of Technology
发明人： Zibo Chen , Michael B. Elowitz
IPC分类号： C12N9/50 , C12N9/06 , C07K14/00
CPC分类号： C12N9/506 , C12Y304/22044 , C12N9/003 , C12Y105/01003 , C07K14/001
摘要： Disclosed herein include methods, compositions, and kits suitable for use in winner-take-all neural network computation in mammalian cells. In some embodiments, de novo designed protein heterodimers and engineered viral proteases are combined to implement a synthetic protein circuit that performs winner-take-all neural network computation. The synthetic protein circuit can include modules that compute weighted sums of input protein concentrations through reversible binding interactions, and allow for self-activation and mutual inhibition of protein components using irreversible proteolytic cleavage reactions.

33. 发明授权

US11781146B2 Vector including a translation-impaired dihydrofolate reductase gene cassette and ubiquitously acting chromatin opening element 有权
公开(公告)号：US11781146B2
公开(公告)日：2023-10-10
申请号：US17054935
申请日：2019-05-13
申请人： National University Corporation Hokkaido University , Fuso Pharmaceutical Industries, Ltd.
发明人： Yasuhiko Suzuki , Miki Nakagawa , Yayoi Kameda , Satoru Konnai , Tomohiro Okagawa , Naoya Maekawa , Shinya Goto , Yamato Sajiki , Kazuhiko Ohashi , Shiro Murata , Yuzuru Kitahara , Keiichi Yamamoto
IPC分类号： C12N15/67 , C12N15/66 , C12N15/85
CPC分类号： C12N15/67 , C12N15/66 , C12N15/85 , C12Y105/01003
摘要： The present invention provides mammalian cell expression vectors that impart to mammalian host cells an ability to produce high levels of foreign gene-derived proteins. A ubiquitously acting chromatin opening element (UCOE) is introduced into an expression vector that has a plasmid DNA integrated into a transcriptional hot spot on the chromosome of a dihydrofolate reductase gene-deficient host cell so that it allows for selection of strains that grow in hypoxanthine-thymidine (hereinafter denoted as HT)-free medium, whereby transformants will produce a protein of interest in increased amounts.

34. 发明申请

US20180265891A1 EXPRESSION VECTOR SYSTEM COMPRISING TWO SELECTION MARKERS 审中-公开
公开(公告)号：US20180265891A1
公开(公告)日：2018-09-20
申请号：US15988141
申请日：2018-05-24
申请人： Novartis AG
发明人： Thomas Jostock , Hans-Peter Knopf
IPC分类号： C12N15/85 , C12N15/52 , C12N1/20 , C12N9/06 , C12N15/79 , C12P21/02
CPC分类号： C12N15/85 , C12N1/20 , C12N9/003 , C12N15/52 , C12N15/79 , C12N2800/40 , C12P21/02 , C12Y105/01003
摘要： The invention pertains to an expression vector or a combination of at least two expression vectors comprising at least (a) a polynucleotide encoding a product of interest or an insertion site for incorporating a polynucleotide encoding a product of interest; (b) a polynucleotide encoding a first selectable marker (sm I); (c) a polynucleotide encoding a second selectable marker (sm II), which is different from the first selectable marker (sm I), wherein the activity of the selectable marker (sm I) or (sm II) is at least partially influenced by the activity of the other selectable marker and wherein the selectable markers (sm I) and (sm II) are involved in the folate metabolism. Also provided are suitable host cells, selection methods and methods for producing polypeptides with high yield.

35. 发明授权

US09913822B2 Combination therapy with an anti-hyaluronan agent and therapeutic agent 有权
公开(公告)号：US09913822B2
公开(公告)日：2018-03-13
申请号：US13815804
申请日：2013-03-15
申请人： Daniel C. Maneval , H. Michael Shepard , Curtis B. Thompson
发明人： Daniel C. Maneval , H. Michael Shepard , Curtis B. Thompson
IPC分类号： A61K47/48 , A61K31/56 , A61K31/7068 , A61K31/337 , A61K38/47 , A61K31/37 , A61K31/42 , A61K31/573 , A61K31/706 , A61K31/7076 , A61K31/7088 , C12N9/06 , C12N9/26 , A61K45/06 , A61K9/00 , A61K47/60 , A61K47/56 , A61K47/64
CPC分类号： A61K31/337 , A61K9/0019 , A61K31/37 , A61K31/42 , A61K31/56 , A61K31/573 , A61K31/706 , A61K31/7068 , A61K31/7076 , A61K31/7088 , A61K38/47 , A61K45/06 , A61K47/48215 , A61K47/48284 , A61K47/56 , A61K47/60 , A61K47/643 , C12N9/003 , C12N9/2474 , C12Y105/01003 , C12Y302/01035 , A61K2300/00
摘要： Provided herein is combination therapy containing an anti-hyaluronan agent, such as a polymer-conjugated hyaluronan-degrading enzyme, and a tumor-targeted taxane, and optionally a further chemotherapeutic agent such as a nucleoside analog. The combination therapy can be used in methods of treating cancers, and in particular solid tumor cancers.

36. 发明申请

US20170290796A1 COMBINATION THERAPY WITH AN ANTI-HYALURONAN AGENT AND THERAPEUTIC AGENT 审中-公开
公开(公告)号：US20170290796A1
公开(公告)日：2017-10-12
申请号：US15625177
申请日：2017-06-16
申请人： Halozyme, Inc.
发明人： Daniel C. Maneval , H. Michael Shepard , Curtis B. Thompson
IPC分类号： A61K31/337 , C12N9/26 , A61K9/00 , C12N9/06 , A61K31/56 , A61K31/7076 , A61K38/47 , A61K31/37
CPC分类号： A61K31/337 , A61K9/0019 , A61K31/37 , A61K31/42 , A61K31/56 , A61K31/573 , A61K31/706 , A61K31/7068 , A61K31/7076 , A61K31/7088 , A61K38/47 , A61K45/06 , A61K47/56 , A61K47/60 , A61K47/643 , C12N9/003 , C12N9/2474 , C12Y105/01003 , C12Y302/01035 , A61K2300/00
摘要： Provided herein is combination cancer therapy effected by administering a polymer-conjugated hyaluronidase, and a tumor-targeted taxane, and optionally a further chemotherapeutic agent such as a nucleoside analog. The combination therapy can be used in methods of treating cancers, and in particular solid tumor cancers.

37. 发明授权

US09593356B2 Protein enriched microvesicles and methods of making and using the same 有权
标题翻译：富含蛋白质的微泡及其制备及使用方法
公开(公告)号：US09593356B2
公开(公告)日：2017-03-14
申请号：US14278714
申请日：2014-05-15
申请人： Clontech Laboratories, Inc.
发明人： Michael Haugwitz , Thomas Patrick Quinn , Andrew Alan Farmer , Montserrat Morell Fernández
IPC分类号： C07K17/06 , C12P21/02
CPC分类号： C12N5/0602 , C07K14/005 , C07K14/43595 , C07K17/06 , C07K2319/00 , C07K2319/70 , C12N5/00 , C12N9/003 , C12N9/1241 , C12N2501/998 , C12N2760/00022 , C12N2760/20222 , C12P21/02 , C12Y105/01003
摘要： Protein enriched micro-vesicles and methods of making and using the same are provided. Aspects of the methods include maintaining a cell having a membrane-associated protein comprising a first dimerization domain and a target protein having a second dimerization domain under conditions sufficient to produce a micro-vesicle from the cell, wherein the micro-vesicle includes the target protein. Also provided are cells, reagents and kits that find use in making the micro-vesicles, as well as methods of using the micro-vesicles, e.g., in research and therapeutic applications.
摘要翻译：提供富含蛋白质的微囊泡及其制备及使用方法。方法的方面包括在足以从细胞产生微囊泡的条件下维持具有包含第一二聚化结构域的膜相关蛋白的细胞和具有第二二聚化结构域的靶蛋白，其中所述微囊包括靶蛋白。还提供了用于制备微囊泡的细胞，试剂和试剂盒，以及使用微囊泡的方法，例如在研究和治疗应用中。

38. 发明授权

US09534226B2 Expression vector system comprising two selection markers 有权
标题翻译：包含两个选择标记的表达载体系统
公开(公告)号：US09534226B2
公开(公告)日：2017-01-03
申请号：US13203610
申请日：2010-02-26
申请人： Thomas Jostock , Hans-Peter Knopf
发明人： Thomas Jostock , Hans-Peter Knopf
IPC分类号： C12P1/00 , C12N15/79 , C12N1/20 , C12P21/02
CPC分类号： C12N15/85 , C12N1/20 , C12N9/003 , C12N15/52 , C12N15/79 , C12N2800/40 , C12P21/02 , C12Y105/01003
摘要： The invention pertains to an expression vector or a combination of at least two expression vectors comprising at least (a) a polynucleotide encoding a product of interest or an insertion site for incorporating a polynucleotide encoding a product of interest; (b) a polynucleotide encoding a first selectable marker (sm I); (c) a polynucleotide encoding a second selectable marker (sm II), which is different from the first selectable marker (sm I), wherein the activity of the selectable marker (sm I) or (sm II) is at least partially influenced by the activity of the other selectable marker and wherein the selectable markers (sm I) and (sm II) are involved in the folate metabolism. Also provided are suitable host cells, selection methods and methods for producing polypeptides with high yield.
摘要翻译：本发明涉及表达载体或至少两种表达载体的组合，所述表达载体至少包含（a）编码感兴趣的产物的多核苷酸或用于掺入编码感兴趣的产物的多核苷酸的插入位点; （b）编码第一选择标记（sm I）的多核苷酸; （c）编码与第一选择标记（sm I）不同的第二选择标记（sm II）的多核苷酸，其中选择性标记（sm I）或（sm II）的活性至少部分地受其他选择标记的活性，其中选择性标记（sm I）和（sm II）参与叶酸代谢。还提供了适合的宿主细胞，用于以高产率生产多肽的选择方法和方法。

39. 发明授权

US09212367B2 Methods for improving recombinant protein expression 有权
标题翻译：改善重组蛋白表达的方法
公开(公告)号：US09212367B2
公开(公告)日：2015-12-15
申请号：US13390297
申请日：2010-08-06
申请人： Howard R. Clarke
发明人： Howard R. Clarke
IPC分类号： C12N15/67 , C12N9/06
CPC分类号： C12N15/67 , C12N9/003 , C12Y105/01003
摘要： Materials and methods are provided which allowed for increased expression of a transfected gene of interest in a recombinant host cell.
摘要翻译：提供了允许在重组宿主细胞中增加感兴趣的基因的表达的材料和方法。

40. 发明授权

US5422275A Protein production at synthetic start site 失效
标题翻译：合成起始位点的蛋白质生产
公开(公告)号：US5422275A
公开(公告)日：1995-06-06
申请号：US163998
申请日：1993-12-08
申请人： Jack H. Nunberg , Annie C. Y. Chang , Stanley N. Cohen , Robert T. Schimke
发明人： Jack H. Nunberg , Annie C. Y. Chang , Stanley N. Cohen , Robert T. Schimke
IPC分类号： C12N9/06 , C12N15/00 , C12N15/70
CPC分类号： C12Y105/01003 , C12N15/00 , C12N9/003
摘要： Methods and compositions are provided for producing heterologous proteins in a micrcorganism host, particularly bacterium, without fusion to an endogenous protein. Particularly, the heterologous gene may be inserted into a vector comprising an endogenous gene, where the heterologous gene is preceded by a ribosomal binding site. A heterologous enzyme functional in a bacterial host is demonstrated.
摘要翻译：提供了用于在微生物宿主，特别是细菌中产生异源蛋白的方法和组合物，而不与内源性蛋白质融合。特别地，异源基因可以插入到包含内源基因的载体中，其中异源基因之前是核糖体结合位点。证明在细菌宿主中功能异源的酶。

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