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31. 发明授权

US11066659B2 Glucose isomerase 有权
公开(公告)号：US11066659B2
公开(公告)日：2021-07-20
申请号：US16610373
申请日：2018-05-04
申请人： C-LEcta GmbH , New Matterhorn, LLC
发明人： Birgit Brucher , Andreas Vogel , Hanna Maria Dudek , Rico Czaja
IPC分类号： C12N9/92 , C12P19/02 , C12P19/24
摘要： The present invention relates to polypeptide, preferably to a glucose isomerase, comprising an amino acid sequence, wherein the amino acid sequence of the polypeptide, preferably the glucose isomerase, is at least 95% identical to an amino acid sequence of SEQ ID NO:1, wherein the amino acid sequence of the polypeptide, preferably the glucose isomerase, comprises an amino acid substitution at one or more amino acid positions, wherein the one or more amino acid positions is/are each and independently selected from the group consisting of SEQ ID NO: 1 amino acid positions 89, 90, 95, 0, 33, 34, 35, and 59. The present invention further relates to methods preparing glucose including the use of the polypeptides of the invention for preparing glucose.

32. 发明申请

US20130288315A1 Nitrilases 有权
标题翻译：腈水解酶
公开(公告)号：US20130288315A1
公开(公告)日：2013-10-31
申请号：US13878979
申请日：2011-10-12
申请人： Andreas Vogel , Daniel Schwarze , Thomas Greiner-Stoeffele
发明人： Andreas Vogel , Daniel Schwarze , Thomas Greiner-Stoeffele
IPC分类号： C12N9/78
CPC分类号： C12N9/78 , C07K14/195 , C12P7/40 , C12P7/42 , C12P13/002 , C12Y305/05001
摘要： The invention relates to a nitrilase having improved activity in the reaction of a nitrile to form the corresponding carboxylic acid, in particular with respect to reacting 2-methylglutaronitrile, 1-(cyanomethyl)cyclohexane-1-carbonitrile, and benzonitrile. The nitrilase according to the invention is related to nitrilase from acidovorax facilis.
摘要翻译：本发明涉及在腈的反应中具有改善的活性以形成相应的羧酸的腈水解酶，特别是关于2-甲基戊二腈，1-（氰基甲基）环己烷-1-甲腈和苄腈的反应。根据本发明的腈水解酶与来自酸酵母的腈水解酶有关。

33. 发明申请

US20110152126A1 Process for Generating a Variant Library of DNA Sequences 有权
标题翻译：生成DNA序列变体库的过程
公开(公告)号：US20110152126A1
公开(公告)日：2011-06-23
申请号：US12960061
申请日：2010-12-03
申请人： Thomas GREINER-STOEFFELE , Claudi FELLER , Marc STRUHALLA
发明人： Thomas GREINER-STOEFFELE , Claudi FELLER , Marc STRUHALLA
IPC分类号： C40B40/08 , C40B50/06
CPC分类号： C12N15/1093 , C12N15/102
摘要： A method for generating a variant library of DNA sequences starting from at least one DNA starting sequence and including the following steps: a) selecting at least two mutation sites in the starting sequence; b) dividing the DNA starting sequence into at least two sequence segments in such a way that at least two of these sequence segments each contain at least one of the mutation sites; c) amplifying the sequence segments by polymerase chain reaction with the aid of a total of at least five different oligonucleotides, where at (i) least one of the oligonucleotides can attach to each of the mutation sites; (ii) at least two of the oligonucleotides can attach to at least one mutation site, and (iii) mutations are introduced, via mismatch positions, into the PCR amplificates by the oligonucleotides at the mutation sites where at least two mutations are introduced at least one of the mutation sites; and d) linking the amplificates to give DNA sequences.
摘要翻译：一种从至少一个DNA起始序列开始产生DNA序列的变体文库的方法，包括以下步骤：a）选择起始序列中的至少两个突变位点; b）将DNA起始序列分成至少两个序列片段，使得这些序列片段中的至少两个各自含有至少一个突变位点; c）通过总共至少五种不同寡核苷酸的聚合酶链反应扩增序列片段，其中（i）至少一种寡核苷酸可以连接到每个突变位点; （ii）至少两个寡核苷酸可以连接至至少一个突变位点，和（iii）通过错配位置将突变引入至少两个突变至少两个突变的突变位点处的PCR扩增片段其中一个突变位点; 和d）连接扩增产生DNA序列。

34. 发明申请

US20110052758A1 HEAT-STABLE ASPARAGINASE FOR REDUCING ACRYLAMIDE IN FOOD STUFFS OR STIMULANTS 有权
标题翻译：用于减少食品中的丙烯酰胺的耐热ASPARAGINASE或STIMULANTS
公开(公告)号：US20110052758A1
公开(公告)日：2011-03-03
申请号：US12663800
申请日：2008-06-12
申请人： Thomas Greiner-Stoeffele , Marc Struhalla
发明人： Thomas Greiner-Stoeffele , Marc Struhalla
IPC分类号： A23F5/16 , C12N15/63 , C12N9/78 , A23F5/00
CPC分类号： A23F5/163 , A21D8/042 , A23F5/02 , A23F5/22 , A23G1/02 , A23L5/25 , A23L19/18 , A23L33/17 , C12N9/82 , C12Y305/01001
摘要： The invention relates to the use of an amidohydrolase for preparing foodstuffs or stimulants.
摘要翻译：本发明涉及用于制备食品或兴奋剂的酰胺水解酶的用途。

35. 发明申请

WO2022002918A1 ONE-POT CELL-FREE GLYCOSYLATION PROCESS 审中-公开
公开(公告)号：WO2022002918A1
公开(公告)日：2022-01-06
申请号：PCT/EP2021/067816
申请日：2021-06-29
申请人： C-LECTA GMBH
发明人： VOGEL, Andreas , FELLER, Claudia , BAYER, Christopher David , HOFFMANN, Gregor , SCHÖNERT, Stefan
IPC分类号： C12P19/04 , C12P19/18 , C12P19/30 , C12P19/32 , C12P19/44 , C12P19/56 , C12P19/305
摘要： The invention relates to a process for the preparation of a glycosylated organic compound by in vitro glycosylation of an organic compound having a nucleophilic group with a saccharide under catalysis of a Leloir glycosyltrans-ferase system comprising at least a first glycosyl transferase and a second glycosyl transferase. The invention further relates to a composition comprising a glycosylated organic compound that is obtainable by the process according to the invention.

36. 发明申请

WO2016198665A1 TRANSAMINASES 审中-公开
标题翻译：转氨酶
公开(公告)号：WO2016198665A1
公开(公告)日：2016-12-15
申请号：PCT/EP2016/063393
申请日：2016-06-10
申请人： C-LECTA GMBH
发明人： VOGEL, Andreas , SCHWARZE, Daniel , CZAJA, Rico , BAYER, Sally , BARTSCH, Sebastian
IPC分类号： C12N9/10
CPC分类号： C12N9/1096
摘要： The invention relates to transaminases and their use. The ATAs according to the invention are particularly useful for catalyzing the conversion of amines to ketones and/or vice versa. Preferably, the transaminase (ATA) according to the invention comprises an amino acid sequence with at least 80% homology to SEQ ID NO:1, wherein the amino acid sequence is engineered compared to SEQ ID NO:1 such that it comprises at least a substitution selected from the group consisting of F255L,F255A, F255C, F255D, F255E, F255G, F255H, F255K, F255M, F255N, F255P, F255Q, F255R, F255S, F255T, F255V, F255W, and F255Y.
摘要翻译：本发明涉及转氨酶及其用途。根据本发明的AT特别可用于催化胺转化为酮和/或反之亦然。优选地，根据本发明的转氨酶（ATA）包含与SEQ ID NO：1具有至少80％同源性的氨基酸序列，其中与SEQ ID NO：1相比，氨基酸序列被工程化，使得其包含至少一个选自F255L，F255A，F255C，F255D，F255E，F255G，F255H，F255K，F255M，F255N，F255P，F255Q，F255R，F255S，F255T，F255V，F255W和F255Y的取代基。

37. 发明申请

WO2013102619A3 VERFAHREN ZUR REDUKTION EINES SECODION-DERIVATS MIT EINER ALKOHOLDEHYDROGENASE 审中-公开
公开(公告)号：WO2013102619A3
公开(公告)日：2013-07-11
申请号：PCT/EP2013/000004
申请日：2013-01-03
申请人： C-LECTA GMBH
发明人： GREINER-STOEFFELE, Thomas , VOGEL, Andreas , SCHMIEDEL, Ramona , KÖPKE, Sabrina
IPC分类号： C12P33/00 , C12N9/02
摘要： Die Erfindung betrifft ein Verfahren zur selektiven Reduktion von Secodion-Derivation zu stereoisomerenreinen einfach reduzierten Verbindungen (Seconolen) mit einem Enzym aus der Klasse der Alkohol-Dehydrogenasen (ADHs), welches eine sowohl regio- als auch stereoselektive Synthese von Seconolen mit hoher Produktreinheit ermöglicht.

38. 发明申请

WO2010075956A1 EXPRESSIONSVEKTOR 审中-公开
标题翻译：表达载体
公开(公告)号：WO2010075956A1
公开(公告)日：2010-07-08
申请号：PCT/EP2009/008977
申请日：2009-12-15
申请人： C-LECTA GMBH , GREINER-STÖFFELE, Thomas , BALLSCHMITTER, Meike , STRUHALLA, Marc , CZAJA, Rico
发明人： GREINER-STÖFFELE, Thomas , BALLSCHMITTER, Meike , STRUHALLA, Marc , CZAJA, Rico
IPC分类号： C12N15/70 , C12N15/10
CPC分类号： C12N15/70 , C12N15/1082 , C12N15/635 , C12N2800/40 , C12N2830/002
摘要： Die Erfindung betrifft einen Expressionsvektor umfassend zwei getrennt voneinander induzierbare, aufeinander zulaufende Promotoren P 1 und P 2 , ein Expressionssystem, welches diesen Expressionsvektor und einen zusätzlichen Regulatorvektor umfasst, Verfahren zur Proteinexpression unter Einsatz dieses Expressionssystems und Verfahren zur Durchmusterung von (Meta)genom-Bibliotheken unter Einsatz dieses Expressionssystems.
摘要翻译：本发明涉及一种包括两个分开的诱导型启动子会聚P1和P2，表达系统，其包括该表达载体和一个额外的调节载体的表达载体，使用该表达系统和方法的（甲基）基因组文库的筛选用于蛋白质表达的方法，使用该表达系统。

39. 发明申请

WO2009141073A1 WASCH- UND REINIGUNGSMITTEL 审中-公开
标题翻译：洗涤和清洁剂
公开(公告)号：WO2009141073A1
公开(公告)日：2009-11-26
申请号：PCT/EP2009/003348
申请日：2009-05-12
申请人： CLARIANT INTERNATIONAL LTD , C-LECTA GMBH , REINHARDT, Gerd , SCHOLZ, Hans, Jürgen , CZAJA, Rico , GREINER-STOEFFELE, Thomas , STRUHALLA, Marc
发明人： REINHARDT, Gerd , SCHOLZ, Hans, Jürgen , CZAJA, Rico , GREINER-STOEFFELE, Thomas , STRUHALLA, Marc
IPC分类号： C11D3/386
CPC分类号： C11D3/38636
摘要： Es werden Wasch- und Reinigungsmittel beschrieben, die eine Kombination aus Chlorophyllase und einer weiteren Hydrolase, vorzugsweise eine Lipase, insbesondere eine Galactolipase enthalten. Die Kombination aus Chlorophyllase und Galactolipase verbessert die Reinigungsleistung, insbesondere bei Chlorophyll-haltigen Anschmutzungen, im Vergleich zu den beiden Enzymen jeweils allein.
摘要翻译：这将在其中包含叶绿素和另一水解酶，优选的脂肪酶的组合，特别是半乳糖脂酶一个洗涤剂和清洗剂进行说明。叶绿素和半乳糖的结合改善了清洁性能，尤其是对含叶绿素土壤，相比于两种酶，各自单独。

40. 发明授权

US11286474B2 Enzyme products 有权
公开(公告)号：US11286474B2
公开(公告)日：2022-03-29
申请号：US16682511
申请日：2019-11-13
申请人： C-LEcta GmbH
发明人： Stefan Schoenert , Mathias Salomo , Thomas Schultchen , Andreas Vogel , Sabrina Koepke , Sebastian Bartsch , Birgit Brucher , Claudia Feller
IPC分类号： C07K1/34 , C12N9/22 , C12N15/52 , C12N9/12 , C12N15/70 , C12N9/52
摘要： The invention relates to a process for the manufacturing and purification of recombinant enzyme products, in particular of food enzyme products and the use thereof. The invention particularly relates to a process for the processing of enzyme products from a microbial fermentation broth by methods of separation, enzymatic treatment and filtration procedures.

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