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31. 发明授权

US10106600B2 Bispecific antibodies 有权
公开(公告)号：US10106600B2
公开(公告)日：2018-10-23
申请号：US13070582
申请日：2011-03-24
申请人： Ulrich Brinkmann , Rebecca Croasdale , Harald Duerr , Christian Klein , Erhard Kopetzki , Wilma Lau , Joerg Thomas Regula , Juergen Michael Schanzer , Pablo Umana , Katharina Wartha
发明人： Ulrich Brinkmann , Rebecca Croasdale , Harald Duerr , Christian Klein , Erhard Kopetzki , Wilma Lau , Joerg Thomas Regula , Juergen Michael Schanzer , Pablo Umana , Katharina Wartha
IPC分类号： A61K39/00 , C07K16/22 , C07K16/28 , C07K16/46
摘要： The present invention relates to bispecific antibodies, methods for their production, pharmaceutical compositions containing said antibodies, and uses thereof.

32. 发明授权

US09382323B2 Multispecific antibodies comprising full length antibodies and single chain fab fragments 有权
标题翻译：包含全长抗体和单链Fab片段的多特异性抗体
公开(公告)号：US09382323B2
公开(公告)日：2016-07-05
申请号：US12750748
申请日：2010-03-31
申请人： Ulrich Brinkmann , Peter Bruenker , Rebecca Croasdale , Christian Klein , Erhard Kopetzki , Ekkehard Moessner , Joerg Thomas Regula , Juergen Michael Schanzer , Jan Olaf Stracke , Pablo Umana
发明人： Ulrich Brinkmann , Peter Bruenker , Rebecca Croasdale , Christian Klein , Erhard Kopetzki , Ekkehard Moessner , Joerg Thomas Regula , Juergen Michael Schanzer , Jan Olaf Stracke , Pablo Umana
IPC分类号： C07K16/00 , C07K16/28 , C07K16/46 , A61K39/00
CPC分类号： C07K16/2863 , A61K2039/505 , C07K16/00 , C07K16/468 , C07K2317/21 , C07K2317/55 , C07K2317/56 , C07K2317/565 , C07K2317/626 , C07K2317/64 , C07K2317/73 , C07K2319/00
摘要： The present invention relates to multispecific, especially bispecific antibodies comprising full length antibodies and single chain Fab fragments, methods for their production, pharmaceutical compositions containing the antibodies, and uses thereof.
摘要翻译：本发明涉及包含全长抗体和单链Fab片段的多特异性，特别是双特异性抗体，其制备方法，含有该抗体的药物组合物及其用途。

33. 发明申请

US20130336972A1 RECOMBINANT FC-FUSION PROTEIN OF THE FIFTH FIBRONECTIN TYPE III DOMAIN OF DCC 审中-公开
标题翻译： DCC第五类FIBRONECTIN III型域的重组FC融合蛋白
公开(公告)号：US20130336972A1
公开(公告)日：2013-12-19
申请号：US13818465
申请日：2011-08-26
申请人： Christian Klein , Erhard Kopetzki , Gerhard Niederfellner , Agnes Bernet , Celine Delloye-Bourgeois , Patrick Mehlen
发明人： Christian Klein , Erhard Kopetzki , Gerhard Niederfellner , Agnes Bernet , Celine Delloye-Bourgeois , Patrick Mehlen
IPC分类号： C07K16/18
CPC分类号： C07K16/18 , C07K14/70503 , C07K2319/30
摘要： The present invention relates to DCC-fusion proteins, nucleic acid molecules encoding the DCC-fusion proteins, as well as methods for their production and their use in treatment of cancer such as colorectal cancer, NSCLC and metastatic breast cancer. The present invention also relates to methods of treating cancer such as colorectal cancer, NSCLC and metastatic breast cancer by administering DCC-fusion proteins.
摘要翻译：本发明涉及DCC融合蛋白，编码DCC-融合蛋白的核酸分子及其生产方法及其在治疗癌症如结肠直肠癌，NSCLC和转移性乳腺癌中的应用。本发明还涉及通过施用DCC-融合蛋白来治疗癌症如结肠直肠癌，NSCLC和转移性乳腺癌的方法。

34. 发明申请

US20090311725A1 POLYPEPTIDE PRODUCING CELLS 有权
标题翻译：多肽生产细胞
公开(公告)号：US20090311725A1
公开(公告)日：2009-12-17
申请号：US12299918
申请日：2007-05-15
申请人： Josef Endl , Erhard Kopetzki , Oliver Ploettner , Ursula Schwarz , Georg Tiefenthaler
发明人： Josef Endl , Erhard Kopetzki , Oliver Ploettner , Ursula Schwarz , Georg Tiefenthaler
IPC分类号： G01N33/53 , C07H21/00 , C12N5/06 , C12N5/08 , C12N15/63 , C12P21/00 , C12Q1/02
CPC分类号： C12N15/85 , C07K16/00 , C07K16/065 , C07K2319/03
摘要： The current invention describes a nucleic acid comprising in a 5′ to 3′ direction a) a first nucleic acid encoding a heterologous polypeptide without an in frame stop codon, b) a second nucleic acid beginning with a 5′ splice donor site and terminated by a 3′ splice acceptor site comprising an in frame translational stop codon and a polyadenylation signal, and c) a nucleic acid encoding i) at least a fragment of a transmembrane domain, or ii) a signal peptide for a GPI-anchor.
摘要翻译：本发明描述了包含在5'至3'方向的核酸a）编码不具有帧内终止密码子的异源多肽的第一核酸，b）以5'剪接供体位点开始并以 3''剪接受体位点，其包含内部翻译终止密码子和多聚腺苷酸化信号，以及c）编码i）至少跨膜结构域片段的核酸，或ii）GPI锚的信号肽。

35. 发明申请

US20050003485A1 Method for recombinant production of polypeptides 有权
标题翻译：重组生产多肽的方法
公开(公告)号：US20050003485A1
公开(公告)日：2005-01-06
申请号：US10866567
申请日：2004-06-12
申请人： Rainer Gruenbeck , Erhard Kopetzki , Friedrich Popp
发明人： Rainer Gruenbeck , Erhard Kopetzki , Friedrich Popp
IPC分类号： C12N15/09 , C12N1/21 , C12P21/00 , C12P21/02 , C07K14/00
CPC分类号： C12P21/02
摘要： A method for the recombinant production of a polypeptide by expressing a nucleic acid encoding said polypeptide in a microbial host cell, forming in the cytoplasm of said host cell inclusion bodies containing said polypeptide, and isolating, solubilizing and naturing said polypeptide, characterized in that after fermentation the host cell or the host cell content is incubated at a temperature of 40° C. or higher for at least 10 minutes and subsequently insoluble polypeptide is isolated from the host cell, this method providing an improved yield in inclusion bodies containing the desired polypeptide.
摘要翻译：一种通过在微生物宿主细胞中表达编码所述多肽的核酸重组产生多肽的方法，在包含所述多肽的所述宿主细胞包涵体的细胞质中形成，并分离，增溶和变性所述多肽，其特征在于，将宿主细胞或宿主细胞含量发酵在40℃或更高的温度下孵育至少10分钟，随后从宿主细胞中分离不溶性多肽，该方法在含有所需多肽的包涵体中提供改善的产量。

36. 发明授权

US06391571B1 Recombinant inactive avidin mutants 有权
标题翻译：重组无活性抗生物素蛋白突变体
公开(公告)号：US06391571B1
公开(公告)日：2002-05-21
申请号：US09366862
申请日：1999-08-04
申请人： Erhard Kopetzki , Rainer Müller , Richard Engh , Urban Schmitt , Arno Deger , Hans Brandstetter
发明人： Erhard Kopetzki , Rainer Müller , Richard Engh , Urban Schmitt , Arno Deger , Hans Brandstetter
IPC分类号： G01N3353
CPC分类号： C07K14/36 , A61K38/00 , C07K14/465 , G01N33/5306
摘要： The present invention concerns muteins of avidin and streptavidin with a reduced binding affinity for biotin as well as their use as interference elimination reagents in methods for the determination of an analyte e.g. in diagnostic tests such as for example immunoassays and nucleic acid hybridization assays. In addition the invention concerns the use of muteins of avidin and streptavidin as systems capable of regeneration for binding biotin e.g. for the analysis of biotinylated molecules, for examining receptor ligand interactions as well as for the affinity purification of biotinylated molecules.
摘要翻译：本发明涉及抗生物素蛋白和链霉抗生物素蛋白的突变蛋白，其对生物素的结合亲和力降低，以及它们在测定分析物的方法中用作干扰消除试剂。在诊断测试中，例如免疫测定和核酸杂交测定。此外，本发明涉及抗生物素蛋白和链霉抗生物素蛋白的突变蛋白作为能够再生以结合生物素的体系的用途。用于分析生物素化分子，用于检查受体配体相互作用以及生物素化分子的亲和纯化。

37. 发明授权

US6136564A Process for the production of peptides by way of streptavidin fusion proteins 失效
标题翻译：通过链霉抗生物素蛋白融合蛋白制备肽的方法
公开(公告)号：US6136564A
公开(公告)日：2000-10-24
申请号：US68738
申请日：1998-06-25
申请人： Erhard Kopetzki
发明人： Erhard Kopetzki
IPC分类号： C12N15/09 , C07K14/36 , C07K14/58 , C07K14/635 , C07K19/00 , C12N15/62 , A61K38/24 , C07K14/00 , C12N1/20 , C97H21/04
CPC分类号： C07K14/58 , C07K14/36 , C07K14/635 , C12N15/62 , C07K2319/00 , C07K2319/22 , C07K2319/50 , C07K2319/75
摘要： The invention relates to a process for recombinant preparation of peptides by expression of a DNA in micro-organisms, which DNA codes for a fusion protein made of streptavidin and one of the said peptides. Streptavidin and the peptide are bound by a peptide sequence which can be cleaved by an endoproteinase. The process also includes isolation of the insoluble, inactive protein, solublisation of the inactive protein using a denaturant, dilution of the denaturant at a pH value of between 8.5 and 11 until cleaving of the fusion protein by an endoproteinase can take place, cleaving of the fusion protein, lowering of the pH value until streptavin and non-cleaved fusion protein precipitate, and cleaning of the desired peptide from the supernatant. Said process is particularly suitably for producing parathromone and urodilatin and fragments thereof.
摘要翻译： PCT No.PCT / EP96 / 04850 Sec。 371日期：1998年6月25日第 102（e）日期1998年6月25日PCT提交1996年11月6日PCT公布。出版物WO97 / 日期1997年5月22日本发明涉及通过在微生物中表达DNA重组制备肽的方法，该DNA编码由链霉抗生物素蛋白和所述肽之一制成的融合蛋白。链霉亲和素和肽被肽序列结合，肽序列可被内蛋白酶切割。该方法还包括使用变性剂分离不溶性无活性蛋白质，使非变性蛋白质溶解，在8.5至11之间的pH值下稀释变性剂，直到可以通过内蛋白酶切割融合蛋白，切割融合蛋白，降低pH值直至链霉亲和素和未切割的融合蛋白沉淀，并从上清液中清洗所需的肽。所述方法特别适用于产生相关参考物和尿嘧啶及其片段。

38. 发明授权

US5798226A Yeast host strains with defects in N-glycosylation 失效
标题翻译：具有N-糖基化缺陷的酵母宿主菌株
公开(公告)号：US5798226A
公开(公告)日：1998-08-25
申请号：US651323
申请日：1996-05-31
申请人： Ludwig Lehle , Klaus Lehnert , Erhard Kopetzki
发明人： Ludwig Lehle , Klaus Lehnert , Erhard Kopetzki
IPC分类号： C07H21/04 , C07K14/395 , C12N1/16 , C12N1/19 , C12N9/04 , C12N15/00 , C12N15/09 , C12P21/00 , C12P21/02 , C12R1/865 , C12N1/18 , C12N15/67
CPC分类号： C12N9/0006 , C12N1/16 , C12P21/005 , C12R1/85
摘要： Saccharomyces mutants with defects in N-glycosylation which are obtainable by �.sup.3 H!-mannose suicide selection, introduction of one or several selective markers, selection of those strains which, after transformation with the plasmid YEpL/glucose oxidase, secrete 10 mg/l glucose oxidase or more into the culture medium after culture under standard conditions, are allelic to the ngd mutations in Saccharomyces cerevisiae, DSM 7042, DSM 7338, DSM 7160 and/or 7340 and express proteins with a uniform carbohydrate structure.
摘要翻译：具有N-糖基化缺陷的糖酵母突变体，其可以通过[3 H] - 甘露糖自杀选择，引入一个或几个选择性标记，选择在用质粒YEpL /葡萄糖氧化酶转化后分泌10mg / l葡萄糖在标准条件下培养后的氧化酶或更多进入培养基，等同于酿酒酵母，DSM 7042，DSM 7338，DSM 7160和/或7340中的ngd突变，并表达具有均匀碳水化合物结构的蛋白质。

39. 发明授权

US5672691A Recombinant core streptavidin 失效
标题翻译：重组核心链霉亲和素
公开(公告)号：US5672691A
公开(公告)日：1997-09-30
申请号：US434718
申请日：1995-05-04
申请人： Erhard Kopetzki , Rainer Rudolph , Adelbert Grossmann
发明人： Erhard Kopetzki , Rainer Rudolph , Adelbert Grossmann
IPC分类号： C07K14/195 , C07K14/36 , C07K14/41 , C12N1/21 , C12N15/09 , C12N15/31 , C12P21/02 , C12R1/19 , G01N33/50 , G01N33/53 , C07K1/22
CPC分类号： C07K14/36
摘要： The present invention concerns a process for the isolation of recombinant core streptavidin in which host cells are transformed with a DNA coding for core streptavidin, the transformed host cells are cultured under suitable conditions, the DNA coding for core streptavidin is expressed and the recombinant core streptavidin is isolated from the host cells or the culture medium, wherein a DNA coding for core streptavidin is used which has (a) the nucleotide sequence shown in SEQ ID NO. 1 or (b) a nucleotide sequence corresponding to the nucleotide sequence (a) within the scope of the degeneracy of the genetic code.
摘要翻译：本发明涉及分离重组核心链霉抗生物素蛋白的方法，其中宿主细胞用编码核心链霉抗生物素蛋白的DNA转化，转化的宿主细胞在合适的条件下培养，编码核心链霉抗生物素蛋白的DNA被表达，重组核心链霉亲和素从宿主细胞或培养基中分离，其中使用编码核心链亲和素的DNA，其具有（a）SEQ ID NO： 1或（b）对应于遗传密码简并范围内的核苷酸序列（a）的核苷酸序列。

40. 发明授权

US5179003A Process for the production of proteins or protein-containing gene products 失效
标题翻译：生产蛋白质或含蛋白质的基因产品的方法
公开(公告)号：US5179003A
公开(公告)日：1993-01-12
申请号：US293502
申请日：1989-01-04
申请人： Dieter H. Wolf , Erhard Kopetzki , Gunther Schumacher
发明人： Dieter H. Wolf , Erhard Kopetzki , Gunther Schumacher
IPC分类号： C12N9/26 , C12N1/16 , C12N1/18 , C12N1/19 , C12N15/09 , C12N15/81 , C12P21/00 , C12R1/865
CPC分类号： C12N15/81 , Y10S435/94
摘要： The present invention provides a process for the production of proteins or protein-containing gene products by transformation of eukaryotic host cells with a recombinant DNA molecule containing the gene for the desired protein, culturing the cells and isolating the gene product after expression, wherein, as host cells, there is used a yeast strain which is deficient in proteases A and B.
摘要翻译：本发明提供了通过用含有所需蛋白质的基因的重组DNA分子转化真核宿主细胞来产生蛋白质或含蛋白质的基因产物的方法，培养细胞并在表达后分离基因产物，其中，作为宿主细胞，使用缺乏蛋白酶A和B的酵母菌株。

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