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31. 发明专利

AU2010307140A1 Multi-modality contrast and brightfield context rendering for enhanced pathology determination and multi-analyte detection in tissue 未知
公开(公告)号：AU2010307140A1
公开(公告)日：2012-04-05
申请号：AU2010307140
申请日：2010-10-07
申请人： VENTANA MED SYST INC
发明人： GARSHA KARL , PESTANO GARY , OTTER MICHAEL , NAGY ALEXANDRA DEA , NAGLE RAY B , MILLER PHILLIP , FROEHLICH JAN , DAY WILLIAM
IPC分类号： G01N33/483
摘要： Multiple modality contrast can be used to produce images that can be combined and rendered to produce images similar to those produced with wavelength absorbing stains viewed under transmitted white light illumination. Images obtained with other complementary contrast modalities can be presented using engineered color schemes based on classical contrast methods used to reveal the same anatomical structures and histochemistry, thereby providing relevance to medical training and experience. Dark-field contrast images derived from refractive index and fluorescent DAPI counterstain images are combined to produce images similar to those obtained with conventional H&E staining for pathology interpretation. Such multi-modal image data can be streamed for live navigation of histological samples, and can be combined with molecular localizations of genetic DNA probes (FISH), sites of mRNA expression (mRNA-ISH), and immunohistochemical (IHC) probes localized on the same tissue sections, used to evaluate and map tissue sections prepared for imaging mass spectrometry.

32. 发明专利

ES2695101T3 Análisis automatizado de células tumorales circulantes 未知
公开(公告)号：ES2695101T3
公开(公告)日：2019-01-02
申请号：ES12816215
申请日：2012-12-28
申请人： VENTANA MED SYST INC
发明人： PESTANO GARY , DITTAMORE RYAN , GARSHA KARL , OTTER MICHAEL , YUN CHOL , NAGY ALEXANDRA DEA
IPC分类号： G01N33/574 , C12Q1/68
摘要： Un procedimiento de análisis de una muestra que contiene células tumorales circulantes (CTC) usando un instrumento automatizado, que comprende: depositar una muestra de un sujeto con cáncer de próstata sobre un sustrato configurado para su uso con el instrumento automatizado, fijar la muestra con formol al 0,4 % durante 1 a 5 minutos seguido de formol tamponado neutro (FTN) al 10 % durante 5 a 15 minutos y metanol durante 1 a 5 minutos, permeabilizar la muestra con Tween 20 al 0,2 % en solución salina tamponada con fosfato (PBS), retener al menos un 90 % de las CTC en la muestra sobre el sustrato tras la fijación y permeabilización, disponer el sustrato en el instrumento automatizado, recuperar las dianas de moléculas de proteína y ácido nucleico en la muestra, en el que la recuperación se hace mediante el instrumento automatizado, en el que la recuperación comprende: (i) poner en contacto la muestra con reactivos de identificación de CTC, en el que el contacto de la muestra con reactivos de identificación de CTC se hace mediante el instrumento automatizado, (ii) poner en contacto la muestra con sondas de ácido nucleico dirigidas al gen relacionado con ETS (ERG), homólogo de fosfatasa y tensina (PTEN) y centrómero 10 (CEN-10), en el que el contacto de la muestra con las sondas de ácido nucleico se hace mediante el instrumento automatizado, obtener imágenes de la muestra, (iii) localizar las CTC en la muestra sobre el sustrato localizando los reactivos de identificación de CTC, en el que los reactivos de identificación de CTC son reactivos inmunohistoquímicos dirigidos a una proteína CD45, una proteína citoqueratina (CQ), una proteína ERG, un receptor androgénico (RA), una proteína PSMA, o combinaciones de los mismos, en el que la localización de los reactivos inmunohistoquímicos se hace mediante el instrumento automatizado, y (iv) obtener imágenes espectrales de las CTC por localización para detectar las sondas de ácido nucleico, en el que la obtención de imágenes espectrales de las CTC se hace mediante el instrumento automatizado, y analizar la muestra analizando las imágenes espectrales y los reactivos de caracterización de CTC detectados.

33. 发明专利

DK2798355T3 Automatiseret analyse af cirkulerende tumorceller 未知
公开(公告)号：DK2798355T3
公开(公告)日：2018-12-03
申请号：DK12816215
申请日：2012-12-28
申请人： VENTANA MED SYST INC
发明人： PESTANO GARY , DITTAMORE RYAN , OTTER MICHAEL , NAGY ALEXANDRA DEA , GARSHA KARL , YUN CHOL S
IPC分类号： C12Q1/68 , G01N33/574

34. 发明专利

CA2981155A1 METHOD AND SYSTEM FOR ASSESSING STAIN QUALITY FOR IN-SITU HYBRIDIZATION AND IMMUNOHISTOCHEMISTRY 未知
公开(公告)号：CA2981155A1
公开(公告)日：2016-12-01
申请号：CA2981155
申请日：2016-05-25
申请人： VENTANA MED SYST INC
发明人： GARSHA KARL , OTTER MICHAEL , STEVENS BENJAMIN , TAFT JEFFERSON , VENTURA FRANK
IPC分类号： G06K9/00 , G01N1/30
摘要： Immunohistochemistry (IHC) and in situ hybridization (ISH) have the aim of detecting, localizing and quantifying certain analytes for various diagnostic purposes. The quality of the stains which are analyzed may deviate for various reasons. Therefore, the present invention provides a method and system for assessing the stain quality and for establishing objective criteria for assessing the stain quality for application in the fields of in-situ hybridization and immunohistochemistry. In one possible embodiment, the invention comprises the steps of unmixing multi-spectral image data of a tissue specimen to obtain analyte intensity images, each analyte intensity image comprising signals from a single stain, computing metrics based on the analyte intensity images, wherein the metrics are uniformity, distribution and/or dispersion of pixel intensity values in the analyte intensity images and assessing a stain quality of a slide by comparing the computed metrics to pre-determined cutoff values regarding uniformity, distribution and/or dispersion of pixel intensity, wherein the stain quality of the slide is assessed as acceptable if the computed metrics meet or exceed the pre-determined cutoff values, and wherein the stain quality of the slide is assessed as unacceptable if the computed metrics do not meet the pre-determined cutoff values. In order to establish objective criteria for assessing stain quality, in one possible embodiment, the method and system includes the step of deriving cut-off values for uniformity, distribution and/or dispersion of pixel intensity by combining the computed metrics based on the analyte intensity images with pre-established data quantifying the stain quality.

35. 发明专利

AU2010307140B2 Multi-modality contrast and brightfield context rendering for enhanced pathology determination and multi-analyte detection in tissue 未知
公开(公告)号：AU2010307140B2
公开(公告)日：2014-05-15
申请号：AU2010307140
申请日：2010-10-07
申请人： VENTANA MED SYST INC
发明人： GARSHA KARL , PESTANO GARY , OTTER MICHAEL , NAGY ALEXANDRA DEA , NAGLE RAY B , MILLER PHILLIP , FROEHLICH JAN , DAY WILLIAM
IPC分类号： G01N33/483 , G01N21/64 , G02B21/36
摘要： Multiple modality contrast can be used to produce images that can be combined and rendered to produce images similar to those produced with wavelength absorbing stains viewed under transmitted white light illumination. Images obtained with other complementary contrast modalities can be presented using engineered color schemes based on classical contrast methods used to reveal the same anatomical structures and histochemistry, thereby providing relevance to medical training and experience. Dark-field contrast images derived from refractive index and fluorescent DAPI counterstain images are combined to produce images similar to those obtained with conventional H&E staining for pathology interpretation. Such multi-modal image data can be streamed for live navigation of histological samples, and can be combined with molecular localizations of genetic DNA probes (FISH), sites of mRNA expression (mRNA-ISH), and immunohistochemical (IHC) probes localized on the same tissue sections, used to evaluate and map tissue sections prepared for imaging mass spectrometry.

36. 发明专利

AU2012222164A1 Presence of ERG gene rearrangements and protein over-expression in low grade pin (LG-PIN) in prostate biopsies 未知
公开(公告)号：AU2012222164A1
公开(公告)日：2013-08-29
申请号：AU2012222164
申请日：2012-02-24
申请人： VENTANA MED SYST INC
发明人： PESTANO GARY , NAGLE RAY , SATHYANARAYANA UBARADKA , NAGY ALEXANDRA DEA , CORTEZ CONNIE , GARSHA KARL , DITTAMORE RYAN
IPC分类号： C12Q1/68
摘要： The present disclosure relates to methods for the early detection of ERG over-expression, gene rearrangements, and diagnosis and/or prognosis of prostate cancer using a combined ERG IHC-FISH assay. An exemplary method comprises: providing a sample from the subject; detecting the presence or absence in the sample of a gene fusion having a 5' portion from a transcriptional regulatory region of an androgen regulated gene and a 3' portion from an ERG gene by detecting chromosomal rearrangements of genomic DNA using a nucleic acid hybridization technique, and detecting the over-expression of ERG protein using an immunohistochemistry assay wherein the presence in the sample of both the gene fusion and an over expression of ERG is indicative of low-grade PIN in the subject that relates to presence of cancer or is predictive of cancer and wherein said subject has previously been determined as negative for prostate cancer.

37. 发明专利

CA2832328A1 SPECTRAL IMAGING FOR MEASUREMENT OF NUCLEAR PATHOLOGY FEATURES IN CANCER CELLS PREPARED FOR IN SITU ANALYSIS 未知
公开(公告)号：CA2832328A1
公开(公告)日：2012-11-15
申请号：CA2832328
申请日：2012-05-07
申请人： VENTANA MED SYST INC , UNIV CORNELL
发明人： DEMICHELIS FRANCESCA , GARSHA KARL , MILLER PHILLIP C , NAGLE RAY B , OTTER MICHAEL , PESTANO GARY ANTHONY , RUBIN MARK A
IPC分类号： C12Q1/68 , C12Q1/6809 , C40B30/00 , G01N33/574 , G06F19/10 , G06F19/24
摘要： In general, the presently disclosed technology relates to identification of cancer subtypes. More specifically, the technology relates to methods for determining molecular drivers of cancer and/or progression using a multivariate image data and statistical analysis of in-situ molecular markers and morphological characteristics in the same cells of a biological sample suspected of cancer. This analysis takes place after a single acquisition that obtains the molecular and anatomic morphology data in parallel. The analysis compares specific morphological and molecular markers to known samples exhibiting particular genetic drivers of the cancer. This method provides statistical information that allows for an increased confidence in the identification of specific molecular drivers of the cancer.

38. 发明专利

CA2774422A1 MULTI-MODALITY CONTRAST AND BRIGHTFIELD CONTEXT RENDERING FOR ENHANCED PATHOLOGY DETERMINATION AND MULTI-ANALYTE DETECTION IN TISSUE 未知
公开(公告)号：CA2774422A1
公开(公告)日：2011-04-21
申请号：CA2774422
申请日：2010-10-07
申请人： VENTANA MED SYST INC
发明人： GARSHA KARL , PESTANO GARY , OTTER MICHAEL , NAGY ALEXANDRA DEA , NAGLE RAY B , MILLER PHILLIP , FROEHLICH JAN , DAY WILLIAM
IPC分类号： G01N33/483
摘要： Multiple modality contrast can be used to produce images that can be combined and rendered to produce images similar to those produced with wavelength absorbing stains viewed under transmitted white light illumination. Images obtained with other complementary contrast modalities can be presented using engineered color schemes based on classical contrast methods used to reveal the same anatomical structures and histochemistry, thereby providing relevance to medical training and experience. Dark-field contrast images derived from refractive index and fluorescent DAPI counterstain images are combined to produce images similar to those obtained with conventional H&E staining for pathology interpretation. Such multi-modal image data can be streamed for live navigation of histological samples, and can be combined with molecular localizations of genetic DNA probes (FISH), sites of mRNA expression (mRNA-ISH), and immunohistochemical (IHC) probes localized on the same tissue sections, used to evaluate and map tissue sections prepared for imaging mass spectrometry.

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