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    • 35. 发明专利
    • Production of ketocarotenoids with low hydroxylated by-product content, for use e.g. in pigmenting feedstuffs, by culturing genetically modified organisms having modified ketolase activity
    • DE10253112A1
    • 2004-06-03
    • DE10253112
    • 2002-11-13
    • SUNGENE GMBH & CO KGAA
    • SAUER MATTFLACHMANN RALFKLEBSATTEL MARTINSCHOPFER CHRISTEL RENATE
    • A23K1/14A23K1/16A23K1/18A23L1/275C12N9/02C12N15/82C12P23/00A01H5/00C12N15/53
    • Production of ketocarotenoids (I) involves culturing genetically modified organisms having modified ketolase (KLA) activity (compared with wild strains) due to a ketolase (II) containing a specific sequence (A) of 258 aminoacids (given in the specification as SEQ. ID. NO. 2) or a mutant sequence of (A), provided that (A') has at least 42% homology with (A). Production of ketocarotenoids (I) involves culturing genetically modified organisms having modified ketolase (KLA) activity (compared with wild strains) due to a ketolase (II) containing a specific sequence (A) of 258 aminoacids (given in the specification as SEQ. ID. NO. 2) or a sequence (A) derived from (A) by substitution, insertion or deletion of amino acids, provided that (A') has at least 42% homology with (A). Independent claims are included for: (1) genetically modified organisms which: (a) show increased KLA activity compared with wild strains (or into which KLA activity is introduced if the wild strain has no KLA activity), having KLA activity due to (II); and/or (b) contain at least one transgenic nucleic acid encoding (A) or (A') or at least two endogenous nucleic acid sequences encoding (II); (2) new ketolases (II'), which contain: (a) a specific sequence (Ai) of 262 aminoacids (SEQ. ID. NO. 8) or a sequence (Ai') derived from (Ai) by substitution, insertion or deletion, provided that (Ai') has at least 70% homology with (Ai) and that a specific sequence of 262 aminoacids (SEQ. ID. NO. 4) is excluded; (b) a specific sequence (Aii) of 253 aminoacids (SEQ. ID. NO. 6) or a sequence (Aii') derived from (Aii) by substitution, insertion or deletion, provided that (Aii') has at least 70% homology with (Aii); (c) a specific sequence (Aiii) of 253 aminoacids (SEQ. ID. NO. 12) or a sequence (Aiii') derived from (Aiii) by substitution, insertion or deletion, provided that (Aiii') has at least 70% homology with (Aiii) and that SEQ. ID. NO. 4 is excluded; or (d) a specific sequence (Aiv) of 267 aminoacids (SEQ. ID. NO. 49) or a sequence (Aiv') derived from (Aiv) by substitution, insertion or deletion, provided that (Aiv') has at least 50% homology with (Aiv) and that a specific sequence of 267 aminoacids (SEQ. ID. NO. 47) is excluded, where all the sequences are defined in the specification; (3) nucleic acids encoding (II'), provided that a specific sequence of 762 bases (SEQ. ID. NO. 5; sequence defined in the specification) is excluded; and (4) the use as ketolase of proteins which contain SEQ. ID. NO. 4 (or a derived sequence having at least 70% homology with SEQ. ID. No. 4), SEQ. ID. NO. 6 (or a derived sequence having at least 65% homology with SEQ. ID. No. 6) or SEQ. ID. NO. 47 (or a derived sequence having at least 50% homology with SEQ. ID. No. 47) and show KLA activity, where all the sequences are defined in the specification.