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    • 36. 发明公开
    • METHODS AND ORGANISMS FOR UTILIZING SYNTHESIS GAS OR OTHER GASEOUS CARBON SOURCES AND METHANOL
    • 方法和机构使用合成气或其它气态碳源甲醇
    • EP2245137A4
    • 2013-06-12
    • EP09704131
    • 2009-01-22
    • GENOMATICA INC
    • BURK MARKSCHILLING CHRISTOPHE HBURGARD ANTHONYTRAWICK JOHN D
    • C12N1/20C12P11/00C12P21/06
    • C12P7/18C12N9/0008C12N9/0036C12N9/1007C12N9/93C12N15/70C12P19/32Y02E50/343
    • The invention provides a non-naturally occurring microbial organism having an acetyl-CoA pathway and the capability of utilizing syngas or syngas and methanol. In one embodiment, the invention provides a non-naturally occurring microorganism, comprising one or more exogenous proteins conferring to the microorganism a pathway to convert CO, CO2 and/or H2 to acetyl-coenzyme A (acetyl-CoA), methyl tetrahydrofolate (methyl-THF) or other desired products, wherein the microorganism lacks the ability to convert CO or CO2 and H2 to acetyl-CoA or methyl-THF in the absence of the one or more exogenous proteins. For example, the microbial organism can contain at least one exogenous nucleic acid encoding an enzyme or protein in an acetyl-CoA pathway. The microbial organism is capable of utilizing synthesis gases comprising CO, CO2 and/or H2, alone or in combination with methanol, to produce acetyl-CoA. The invention additionally provides a method for producing acetyl-CoA, for example, by culturing an acetyl-CoA producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme or protein in a sufficient amount to produce acetyl-CoA, under conditions and for a sufficient period of time to produce acetyl-CoA.
    • 本发明提供了具有乙酰-CoA途径且利用合成气或合成气和甲醇的能力的非天然存在的微生物有机体。 在一个,实施例本发明提供一种非天然存在的微生物,其包含一种或多种外源蛋白质赋予所述微生物的通路到CO,CO 2和/或H 2转化为乙酰辅酶A(乙酰-CoA),甲基四氢叶酸(甲基 -THF)或其它期望产物,worin所述微生物缺乏将CO或CO 2和H 2转化为乙酰-CoA或甲基 - 四氢叶酸在不存在所述一种或多种外源蛋白质的能力。 例如,所述微生物有机体可包含在到乙酰-CoA途径在酶或蛋白质的至少一种外源核酸编码。 微生物生物体能够利用合成气含有CO,CO 2和/或H 2,单独或与甲醇结合,以产生乙酰-CoA。 本发明另外提供了用于产生乙酰-CoA,例如通过乙酰辅酶A的培养生产微生物,一方法,其中所述微生物生物体快车至少一种外源性核酸编码乙酰-CoA途径酶或蛋白质以足够的量,以 产生乙酰-CoA的条件和时间以产生乙酰-CoA足够长的时间。