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31. 发明授权

US09175298B2 Compositions for enhancing segregation of transgenes in plants 有权
标题翻译：用于增强植物中转基因分离的组合物
公开(公告)号：US09175298B2
公开(公告)日：2015-11-03
申请号：US11857000
申请日：2007-09-18
申请人： Toni Armstrong , Kenneth Barton , Laura J. Crow , Larry A. Gilbertson , Shihshieh Huang , Yong Huang , Brian J. Martinell , Michael W. Petersen , Kenneth J. Piller , Qi Wang , Xudong Ye
发明人： Toni Armstrong , Kenneth Barton , Laura J. Crow , Larry A. Gilbertson , Shihshieh Huang , Yong Huang , Brian J. Martinell , Michael W. Petersen , Kenneth J. Piller , Qi Wang , Xudong Ye
IPC分类号： C12N15/82
CPC分类号： C12N15/8205 , C12N15/8209 , C12N15/8263 , C12N15/8265 , C12N15/8289
摘要： The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independent transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.
摘要翻译：提供的组合物和方法增强了在不同物理和遗传基因座处整合到植物基因组中的具有两个T-DNA分子的转基因植物的选择。组合物是包含含有感兴趣的基因，阳性选择标记基因和条件致死基因的T-DNA分子的新配置的DNA构建体。本文公开的方法包括将植物细胞转化为包含本发明的DNA构建体，将植物细胞再生成植物并鉴定独立的转基因基因座，其中可选择标记基因或转基因元件可以在后代中分离。

32. 发明授权

US09003696B2 Automated systems for removing tissue samples from seeds, and related methods 有权
标题翻译：用于从种子中去除组织样品的自动化系统及相关方法
公开(公告)号：US09003696B2
公开(公告)日：2015-04-14
申请号：US13186126
申请日：2011-07-19
申请人： Kevin L. Deppermann , Michael W. Petersen , Allen N. Ondes , David W. Finley , William M. Fischer , John M. Jensen , David Butruille , Stanton Dotson , Sam Eathington , Heather M. Forbes , Bruce Schnicker , John Tamulonis
发明人： Kevin L. Deppermann , Michael W. Petersen , Allen N. Ondes , David W. Finley , William M. Fischer , John M. Jensen , David Butruille , Stanton Dotson , Sam Eathington , Heather M. Forbes , Bruce Schnicker , John Tamulonis
IPC分类号： A01C1/00 , G01N1/04 , G01N1/20 , G01N1/28
CPC分类号： G01N35/00584 , A01C1/00 , A01C1/025 , G01N1/04 , G01N1/20 , G01N1/286 , G01N33/0098 , G01N33/5097 , G01N2001/002
摘要： A seed sampling system is provided having an automated seed loading assembly including a seed bin and being operable to singulate seeds from a plurality of seeds within the seed bin. The system also includes an automated seed sampling assembly operable to remove tissue samples from the singulated seeds, and an automated seed transport assembly operable to transfer the singulated seeds from the seed loading assembly to the seed sampling assembly. The seed transport assembly includes multiple retention members. Each of the retention members is movable relative to the seed loading assembly and to the seed sampling assembly. The seed transport assembly is operable to position one of the multiple retention members adjacent to the seed loading assembly for engaging one of the singulated seeds, while positioning another of the retention members adjacent to the seed sampling assembly for presenting another of the singulated seeds to the seed sampling assembly.
摘要翻译：提供种子采样系统，其具有包括种子仓的自动种子加载组件，并且可操作以从种子仓内的多个种子中分离种子。该系统还包括可操作以从单个种子中去除组织样本的自动种子取样组件，以及可操作以将单种化种子从种子装载组件转移到种子采样组件的自动种子运输组件。种子运输组件包括多个保持构件。每个保持构件可相对于种子装载组件和种子取样组件移动。种子运输组件可操作以将多个保持构件中的一个定位成邻近种子装载组件以接合单个种子中的一个，同时将另一个保持构件定位成与种子取样组件相邻，以将另一个单个种子呈现到种子采样总成。

33. 发明授权

US08829275B2 Methods and compositions for obtaining marker-free transgenic plants 有权
标题翻译：用于获得无标记转基因植物的方法和组合物
公开(公告)号：US08829275B2
公开(公告)日：2014-09-09
申请号：US13558336
申请日：2012-07-25
申请人： Xudong Ye , Michael W. Petersen , Larry Gilbertson , David Walters , Susan Johnson , Shihshieh Huang , Paul S. Chomet
发明人： Xudong Ye , Michael W. Petersen , Larry Gilbertson , David Walters , Susan Johnson , Shihshieh Huang , Paul S. Chomet
IPC分类号： C12N15/113 , C12N15/82 , C12N15/84 , C12N5/10 , A01H1/04 , A01H1/02 , A01H5/00 , C12N9/10 , C12Q1/68
CPC分类号： C12Q1/6895 , A01H1/04 , C12N9/1085 , C12N15/8205 , C12N15/821 , C12N15/8212 , C12N15/8218 , C12N15/8234 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12N15/8263 , C12N15/8265 , C12N15/8274 , C12N15/8279 , C12N15/8286 , C12Q2600/13 , C12Q2600/156
摘要： The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
摘要翻译：本发明提供用于鉴定含有感兴趣的转基因但缺少标记基因的转基因种子的方法和组合物。使用导致可检测表型的鉴定序列增加筛选未与目的基因连接的转基因序列与编码感兴趣的基因的序列分离的种子和植物的效率。

34. 发明授权

US08809628B2 S-adenosylmethionine synthetase expression elements identified from Arabidopsis thaliana 有权
标题翻译：拟南芥中鉴定的S-腺苷甲硫氨酸合成酶表达元件
公开(公告)号：US08809628B2
公开(公告)日：2014-08-19
申请号：US12781663
申请日：2010-05-17
申请人： Wei Wu , Qi Wang , Michael Edgerton , Kim Beazley , Jill Deikman , Michael W. Petersen , Bei Zhang
发明人： Wei Wu , Qi Wang , Michael Edgerton , Kim Beazley , Jill Deikman , Michael W. Petersen , Bei Zhang
IPC分类号： A01H5/00 , A01H5/10 , A01H1/00
CPC分类号： C12N15/8216 , A01H5/00 , C12N9/1085 , C12N15/8289 , C12Y205/01006
摘要： The present invention provides non-coding regulatory element polynucleotide molecules isolated from the S-adenosyl methionine synthetase (SAMS3) gene of Arabidopsis thaliana and useful for expressing transgenes in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the Arabidopsis thaliana regulatory polynucleotide sequences, and methods for preparing and using the same.
摘要翻译：本发明提供从拟南芥的S-腺苷甲硫氨酸合成酶（SAMS3）基因中分离的非编码调控元件多核苷酸分子，并且可用于在植物中表达转基因。本发明还公开了组合物，多核苷酸构建体，转化的宿主细胞，转基因植物和含有拟南芥调节多核苷酸序列的种子，以及制备和使用拟南芥调节多核苷酸序列的方法。

35. 发明授权

US08436225B2 Automated seed sampler and methods of sampling, testing and bulking seeds 有权
标题翻译：自动种子采样器和采样，测试和膨化种子的方法
公开(公告)号：US08436225B2
公开(公告)日：2013-05-07
申请号：US13302657
申请日：2011-11-22
申请人： Kevin L. Deppermann , Michael W. Petersen
发明人： Kevin L. Deppermann , Michael W. Petersen
IPC分类号： A01H9/00 , A01H11/00
CPC分类号： G01N1/04 , A01C1/00 , A01C1/025 , A01H1/02 , A01H1/04 , A01H4/003 , B07C2501/009 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , G01N35/00029 , G01N35/04
摘要： A population of seeds wherein substantially all of the seeds in the population have a portion of seed tissue removed by an automated sampling system. Substantially all of the seeds in the population have the presence or absence of at least one transgene in common, and/or substantially all of the seeds in the population have a high recurrent parent allele frequency. The presence or absence of the at least one transgene in the seeds in the population and/or the recurrent parent allele frequency in the seeds in the population is determined by analyzing the portion of seed tissue removed from the seeds by the automated sampling system.
摘要翻译：一群种子，其中群体中基本上所有的种子都具有通过自动采样系统去除的一部分种子组织。群体中基本上所有的种子都存在或不存在共同的至少一个转基因，并且/或基本上所有种子中的种子都具有高的亲本亲本等位基因频率。通过分析通过自动采样系统从种子中除去的部分种子组分来确定种群中种子中的至少一种转基因和/或群体中种子中的复发亲本等位基因频率的存在或不存在。

36. 发明申请

US20120131699A1 METHODS AND COMPOSITIONS FOR OBTAINING MARKER-FREE TRANSGENIC PLANTS 有权
标题翻译：获得无标记转基因植物的方法和组合物
公开(公告)号：US20120131699A1
公开(公告)日：2012-05-24
申请号：US13271666
申请日：2011-10-12
申请人： Xudong Ye , Michael W. Petersen , Larry Gilbertson , David Walters , Susan Johnson , Shihshieh Huang , Paul S. Chomet
发明人： Xudong Ye , Michael W. Petersen , Larry Gilbertson , David Walters , Susan Johnson , Shihshieh Huang , Paul S. Chomet
IPC分类号： A01H5/00 , A01H5/10 , C12N1/21 , C12N5/04 , C12N15/52 , C12N15/63
CPC分类号： C12Q1/6895 , A01H1/04 , C12N9/1085 , C12N15/8205 , C12N15/821 , C12N15/8212 , C12N15/8218 , C12N15/8234 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12N15/8263 , C12N15/8265 , C12N15/8274 , C12N15/8279 , C12N15/8286 , C12Q2600/13 , C12Q2600/156
摘要： The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
摘要翻译：本发明提供用于鉴定含有感兴趣的转基因但缺少标记基因的转基因种子的方法和组合物。使用导致可检测表型的鉴定序列增加筛选未与目的基因连接的转基因序列与编码感兴趣的基因的序列分离的种子和植物的效率。

37. 发明申请

US20120079629A1 AUTOMATED SEED SAMPLER AND METHODS OF SAMPLING, TESTING AND BULKING SEEDS 有权
标题翻译：自动种子采样器和采样，测试和播种种子的方法
公开(公告)号：US20120079629A1
公开(公告)日：2012-03-29
申请号：US13302657
申请日：2011-11-22
申请人： Kevin L. Deppermann , Michael W. Petersen
发明人： Kevin L. Deppermann , Michael W. Petersen
IPC分类号： A01H5/10
CPC分类号： G01N1/04 , A01C1/00 , A01C1/025 , A01H1/02 , A01H1/04 , A01H4/003 , B07C2501/009 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , G01N35/00029 , G01N35/04
摘要： A population of seeds wherein substantially all of the seeds in the population have a portion of seed tissue removed by an automated sampling system. Substantially all of the seeds in the population have the presence or absence of at least one transgene in common, and/or substantially all of the seeds in the population have a high recurrent parent allele frequency. The presence or absence of the at least one transgene in the seeds in the population and/or the recurrent parent allele frequency in the seeds in the population is determined by analyzing the portion of seed tissue removed from the seeds by the automated sampling system.
摘要翻译：一群种子，其中群体中基本上所有的种子都具有通过自动采样系统去除的一部分种子组织。群体中基本上所有的种子都存在或不存在共同的至少一个转基因，并且/或基本上所有种子中的种子都具有高的亲本亲本等位基因频率。通过分析通过自动采样系统从种子中除去的部分种子组分来确定种群中种子中的至少一种转基因和/或群体中种子中的复发亲本等位基因频率的存在或不存在。

38. 发明授权

US07830516B2 Automated seed sampler and methods of sampling, testing and bulking seeds 有权
标题翻译：自动种子采样器和采样，测试和膨化种子的方法
公开(公告)号：US07830516B2
公开(公告)日：2010-11-09
申请号：US12243739
申请日：2008-10-01
申请人： Kevin L. Deppermann , Michael W. Petersen
发明人： Kevin L. Deppermann , Michael W. Petersen
IPC分类号： G01N21/25 , G01J3/30 , C12Q1/66
CPC分类号： G01N1/04 , A01C1/00 , A01C1/025 , A01H1/02 , A01H1/04 , A01H4/003 , B07C2501/009 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , G01N35/00029 , G01N35/04
摘要： An automated seed sampler includes a sampling station, a sampler for removing material from a seed in the sampling station; a seed conveyer for conveying the seed from the sampling station to a compartment in a seed tray, and a conveyor for conveying the material removed from the seed to a corresponding compartment in a sample tray. And a method for bulking up a quantity of plant seed having a desired characteristic generally includes testing each seed for the desired characteristic, cultivating plants only from seeds which tested positive for the desired characteristic, recovering seed from the cultivated plants, and repeating the processes of testing, cultivating, and recovering seed for one or multiple generations.
摘要翻译：自动种子取样器包括采样站，用于从采样站中的种子去除材料的采样器; 用于将种子从采样站输送到种子盘中的隔间的种子传送器，以及用于将从种子移除的材料输送到样品托盘中的对应隔室的输送机。并且用于填充一定数量的具有所需特征的植物种子的方法通常包括测试每种种子所需的特性，仅从对所需特征测试为阳性的种子培养植物，从栽培植物中回收种子，并重复测试，培育和恢复一代或多代的种子。

39. 发明申请

US20100196950A1 AUTOMATED SEED SAMPLER AND METHODS OF SAMPLING, TESTING AND BULKING SEEDS 有权
标题翻译：自动种子采样器和采样，测试和播种种子的方法
公开(公告)号：US20100196950A1
公开(公告)日：2010-08-05
申请号：US12759423
申请日：2010-04-13
申请人： Kevin L. Deppermann , Michael W. Petersen
发明人： Kevin L. Deppermann , Michael W. Petersen
IPC分类号： C12Q1/02 , A01H5/00 , C12M1/26
CPC分类号： G01N1/04 , A01C1/00 , A01C1/025 , A01H1/02 , A01H1/04 , A01H4/003 , B07C2501/009 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , G01N35/00029 , G01N35/04
摘要： An automated seed sampler includes a sampling station; a sampler for removing material from a seed in the sampling station; a seed conveyer for conveying the seed from the sampling station to a compartment in a seed tray; and a conveyor for conveying the material removed from the seed to a corresponding compartment in a sample tray. The method of the present invention comprises feeding seeds individually to a sampling station, removing a sample from the seed in the sampling station; conveying the sample to a compartment in a sample tray, and conveying the seed to a corresponding compartment in a seed tray. The samples can be tested, and the seeds can be sorted according to the results of the testing of their corresponding samples.
摘要翻译：自动种子取样器包括采样站; 用于从采样站中的种子去除材料的采样器; 种子输送机，用于将种子从采样站输送到种子盘中的隔室; 以及用于将从种子移除的材料输送到样品托盘中的相应隔室的输送机。本发明的方法包括将种子单独送入采样站，从采样站的种子中取出样品; 将样品输送到样品盘中的隔室，并将种子输送到种子托盘中的相应隔室。可以测试样品，并可以根据其相应样品的测试结果对种子进行分类。

40. 发明申请

US20070288334A1 Administering a contract over a data network 失效
标题翻译：通过数据网络管理合同
公开(公告)号：US20070288334A1
公开(公告)日：2007-12-13
申请号：US10673142
申请日：2003-09-30
申请人： Sherwood Creedle , Michael W. Petersen , Tom Obermeyer , Eric Kukanic
发明人： Sherwood Creedle , Michael W. Petersen , Tom Obermeyer , Eric Kukanic
IPC分类号： G06Q40/00
CPC分类号： G06Q40/00 , G06Q10/06311 , G06Q10/063118 , G06Q10/06315
摘要： A computer-based method for administering performance of a contract uses a server accessible by multiple performing parties through a data network, such as the Internet. The method includes receiving performance data at the server from at least one of the performing parties through the data network. The performance data indicates an amount of work performed under the contract by the performing party, and may further include an amount of materials stored. A payment amount is determined based on the performance data. A schedule of values associated with performance of the contract may be initially stored, so that determining the payment amount is further based on comparing the performance data with the schedule of values. The payment amount is sent from the server to other performing parties through the data network. The other performing parties may be notified by an email indicating, for example, that the payment amount has been approved.
摘要翻译：用于管理合同性能的基于计算机的方法使用由多个执行方通过诸如因特网的数据网络访问的服务器。该方法包括通过数据网从至少一个执行方接收服务器上的性能数据。演出数据表示由履约方根据合同进行的工作量，并且还可以包括存储的材料量。支付金额根据业绩数据确定。可以初始地存储与合同执行相关联的值的调度表，从而进一步基于将性能数据与值的调度进行比较来确定支付金额。支付金额通过数据网络从服务器发送到其他执行方。其他执行方可以通过电子邮件通知，例如指示支付金额已被批准。

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