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    • 39. 发明授权
    • System and method for detection, identification and monitoring of
submicron-sized particles
    • 用于亚微米级颗粒的检测,鉴定和监测的系统和方法
    • US6051189A
    • 2000-04-18
    • US941990
    • 1997-10-01
    • Charles H. WickDavid M. Anderson
    • Charles H. WickDavid M. Anderson
    • G01N1/22G01N1/34G01N9/30G01N27/00
    • G01N9/30G01N1/2202G01N2001/2223G01N2001/4066Y10T436/143333Y10T436/25375Y10T436/25875
    • A system and method for detection, identification, and monitoring of submicron sized particles, the method including the steps of collecting a sample, extracting existing submicron particles from the collected sample based on density, purifying the extracted submicron particles by concentrating the extracted submicron particles based on size, and, detecting and identifying the purified extracted submicron particles based on size and density thereby determining submicron particles present in the collected sample. The submicron particles detected and identified include viruses and virus-like agents such as prions. Thus, virus and virus-like agents can be detected and identified based only on their physical properties without the use of biochemical reagents or assays. A system for carrying out the method of detection and identification of these particles is also disclosed.
    • 一种用于亚微米尺寸颗粒的检测,鉴定和监测的系统和方法,所述方法包括以下步骤:收集样品,基于密度从收集的样品中提取现有的亚微米颗粒,通过浓缩提取的亚微米颗粒来纯化提取的亚微米颗粒 并且基于尺寸和密度检测和鉴定纯化的提取的亚微米颗粒,从而确定收集的样品中存在的亚微米颗粒。 检测和鉴定的亚微米颗粒包括病毒和类似病毒的试剂,例如朊病毒。 因此,病毒和病毒样试剂可以仅基于其物理性质而不使用生物化学试剂或测定法来检测和鉴定。 还公开了一种用于执行这些颗粒的检测和识别方法的系统。
    • 40. 发明授权
    • Method for producing somoclonal variant cotton plants
    • 生产单克隆变异棉花植物的方法
    • US5874662A
    • 1999-02-23
    • US475971
    • 1995-06-07
    • Thirumale S. RanganDavid M. Anderson
    • Thirumale S. RanganDavid M. Anderson
    • A01H1/02A01H4/00C12N15/82C12N15/01A01H1/04A01H1/06
    • A01H4/008A01H1/02A01H4/001A01H4/005C12N15/8223C12N15/8275C12N15/8286
    • A method for producing somaclonal variant cotton plant. The method comprising providing a cotton explant, culturing the explant in a callus growth medium supplemented with glucose as a primary carbon source until secretion of phenolic compounds has ceased and undifferentiated callus is formed from the explant, culturing the undifferentiated callus in callus growth medium supplemented with sucrose as primary carbon source until embryogenic callus is formed from the undifferentiated callus, transferring the embryogenic callus to a plant germination medium, culturing the embryogenic callus on the plant germination medium until a plantlet is formed from the embryogenic callus, transferring the plantlets to soil, growing the plantlets to produce seeds from self pollination, collecting the seeds, planting the seeds, growing the seeds under conditions to select for a desired characteristic and collecting the plants with the desired characteristics.
    • 一种生产体细胞变异棉花植物的方法。 所述方法包括提供棉花外植体,在补充有葡萄糖作为主要碳源的愈伤组织生长培养基中培养外植体,直到酚类化合物的分泌已经停止,并且从外植体形成未分化的愈伤组织,培养未分化愈伤组织的愈伤组织生长培养基中补充有 蔗糖作为主要碳源,直到从未分化的愈伤组织形成胚性愈伤组织,将胚性愈伤组织转移到植物发芽培养基上,在植物发芽培养基上培养胚性愈伤组织,直到从胚发生愈伤组织形成小植株,将植物转移到土壤中, 生长种子以从自花授粉种子,收集种子,种植种子,在条件下种植种子以选择期望的特征并收集具有所需特征的植物。