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31. 发明授权

US5191063A Production of biologically active polypeptides by treatment with an exogenous peptide sequence 失效
标题翻译：通过用异源肽序列处理生产生物活性多肽
公开(公告)号：US5191063A
公开(公告)日：1993-03-02
申请号：US346552
申请日：1989-05-02
申请人： Masayori Inouye , Yoshiji Ohta , Xueli Zhu , Frank Jordan
发明人： Masayori Inouye , Yoshiji Ohta , Xueli Zhu , Frank Jordan
IPC分类号： C12N9/00 , C07K1/00 , C07K1/113 , C07K14/575 , C07K14/62 , C07K14/745 , C07K14/76 , C12N9/56 , C12P21/00 , C12P21/02
CPC分类号： C07K1/1133 , Y10S435/839
摘要： A method of making a biologically inactive polypeptide active is disclosed. Activity is imparted to the polypeptide through treatment with an exogenous peptide sequence. The nature of the exogenous peptide sequence is disclosed.

32. 发明授权

US5079151A Production of branched RNA-linked multi-copy single-stranded DNA using permeabilized cells 失效
标题翻译：使用透化细胞生产支链RNA连接的多拷贝单链DNA
公开(公告)号：US5079151A
公开(公告)日：1992-01-07
申请号：US315427
申请日：1989-02-24
申请人： Bert C. Lampson , Masayori Inouye , Sumiko Inouye
发明人： Bert C. Lampson , Masayori Inouye , Sumiko Inouye
IPC分类号： C07H21/04 , C12N15/09 , C12N15/10 , C12P19/34 , C12R1/01
CPC分类号： C12N15/10 , C12P19/34 , Y10S435/822
摘要： msDNA is a peculiar molecule consisting of a branched RNA linked to single-stranded DNA via a 2', 5' phosphodiester bond. A cell-free system, utilizing cells permeablized with phenethyl, alcohol, was established to study the synthesis of msDNA in Myxococcus xanthus. Permeablized cell labeled with [.alpha.-.sup.32 P]dCTP in the presence of ddGTP, ddATP, or ddTTP, produce a band that migrates at the same position as the full-sized msDNA in an acrylamide gel. However, when this band is treated with ribonuclease A prior to gel electrophoresis, it resulted in many bands of different sizes. This indicates that during the labeling, intermediates are produced in which single-stranded DNAs of various lengths are associated with a compensatory length of RNA such that the total length for each intermediate is identical. These results provide evidence for the previously proposed model in which msDNA is synthesized by reverse transcriptase using a folded RNA precursor as a primer as well as a template. Furthermore, it was found that there is a precise coupling mechanism of reverse transcriptase and ribonuclease H.
摘要翻译： msDNA是由通过2'，5'磷酸二酯键与单链DNA连接的支链RNA组成的特殊分子。建立了一种无细胞系统，利用渗透有苯乙醇，醇的细胞，研究了黄连球菌中msDNA的合成。在ddGTP，ddATP或ddTTP存在下用[α-32P] dCTP标记的渗透细胞产生在与丙烯酰胺凝胶中的全尺寸msDNA相同位置迁移的条带。然而，当在胶束电泳之前用核糖核酸酶A处理该条带时，其产生许多不同大小的条带。这表明在标记过程中产生了中间体，其中各种长度的单链DNA与代偿性长度的RNA相关联，使得每个中间体的总长度相同。这些结果为先前提出的模型提供了证据，其中使用折叠的RNA前体作为引物以及模板通过逆转录酶合成了msDNA。此外，发现有逆转录酶和核糖核酸酶H的精确偶联机制。

33. 发明授权

US09243234B2 Sequence-specific MRNA interferase and uses thereof 有权
标题翻译：序列特异性MRNA干扰素及其用途
公开(公告)号：US09243234B2
公开(公告)日：2016-01-26
申请号：US14236947
申请日：2012-08-03
申请人： Masayori Inouye , Yoshihiro Yamaguchi
发明人： Masayori Inouye , Yoshihiro Yamaguchi
IPC分类号： C12N9/22 , C12Q1/68
CPC分类号： C12N9/22 , C12Q1/6869
摘要： The present invention provides an improved and specific mRNA interferase and related methods of protein-based mRNA interference.
摘要翻译：本发明提供了一种改进的和特异性的mRNA干扰酶以及基于蛋白质的mRNA干扰的相关方法。

34. 发明申请

US20140294801A1 SEQUENCE-SPECIFIC MRNA INTERFERASE AND USES THEREOF 有权
标题翻译：序列特异性MRNA干扰及其用途
公开(公告)号：US20140294801A1
公开(公告)日：2014-10-02
申请号：US14236947
申请日：2012-08-03
申请人： Masayori Inouye , Yoshihiro Yamaguchi
发明人： Masayori Inouye , Yoshihiro Yamaguchi
IPC分类号： C12N9/22 , C12Q1/68
CPC分类号： C12N9/22 , C12Q1/6869
摘要： The present invention provides an improved and specific mRNA interferase and related methods of protein-based mRNA interference.
摘要翻译：本发明提供了一种改进的和特异性的mRNA干扰酶以及基于蛋白质的mRNA干扰的相关方法。

35. 发明申请

US20110306751A1 Independently Inducible System of Gene Expression 审中-公开
标题翻译：独立诱导的基因表达系统
公开(公告)号：US20110306751A1
公开(公告)日：2011-12-15
申请号：US13122547
申请日：2009-10-05
申请人： Monica J. Roth , William M. Schneider , Gaetano T. Montelione , Masayori Inouye , Yuefeng Tang
发明人： Monica J. Roth , William M. Schneider , Gaetano T. Montelione , Masayori Inouye , Yuefeng Tang
IPC分类号： C07K2/00 , C12N1/21 , C12P21/00
CPC分类号： C12N15/72 , C07K14/31 , C07K2319/02 , C12N1/20 , C12N9/22 , C12N15/635 , C12N15/67 , C12Y301/00 , H05K999/99
摘要： The present invention is directed to the improved methods for the temporal induction of proteins using the condensed single protein production (cSPP) system.
摘要翻译：本发明涉及使用缩合单蛋白质生产（cSPP）系统来时间诱导蛋白质的改进方法。

36. 发明申请

US20100234287A1 Targeting Bacterial Suicide Pathways for the Development of Novel Antibiotics 审中-公开
标题翻译：针对新型抗生素开发的细菌自杀途径
公开(公告)号：US20100234287A1
公开(公告)日：2010-09-16
申请号：US12293838
申请日：2007-03-22
申请人： Masayori Inouye , Nancy Woychik , Koichi Inoue
发明人： Masayori Inouye , Nancy Woychik , Koichi Inoue
IPC分类号： A61K38/00 , C12Q1/68 , C12N1/20 , A61K38/16 , C07K2/00 , C07K14/00
CPC分类号： C12Q1/6818
摘要： The invention provides methods for identifying an agent which prevents or partially prevents an antitoxin from forming a complex with its cognate toxin, comprising contacting a potential agent with a labeled substrate in solution, whereby detection of the label indicates presence of an agent that prevents an antitoxin from forming complex with a toxin. The invention also provides agents capable of interfering with formation of a toxin-antitoxin complex. Such agents act as novel, non-conventional antibiotics against human pathogenic bacteria.
摘要翻译：本发明提供用于鉴定防止或部分阻止抗毒素与其同源毒素形成复合物的试剂的方法，包括将潜在试剂与溶液中的标记底物接触，由此检测标记表示存在防止抗毒素的试剂从与毒素形成复合物。本发明还提供能够干扰毒素 - 抗毒素复合物形成的试剂。这些药物作为抗人类病原菌的新型非常规抗生素。

37. 发明申请

US20100196951A1 Antitoxin Destabilization Technology 审中-公开
标题翻译：抗毒素不稳定技术
公开(公告)号：US20100196951A1
公开(公告)日：2010-08-05
申请号：US12668841
申请日：2008-07-14
申请人： Masayori Inouye , Koichi Inoue
发明人： Masayori Inouye , Koichi Inoue
IPC分类号： C12P21/00 , C07K1/14 , C12N15/74
CPC分类号： C07K14/245
摘要： Disclosed are methods for purifying toxin proteins which avoid denaturation and renaturation procedures.
摘要翻译：公开了用于纯化毒素蛋白质的方法，其避免变性和复性程序。

38. 发明授权

US07605000B2 Cold shock inducible expression and production of heterologous polypeptides 失效
标题翻译：冷休克诱导表达和产生异源多肽
公开(公告)号：US07605000B2
公开(公告)日：2009-10-20
申请号：US10506192
申请日：2003-02-25
申请人： Masayori Inouye , Sangita Phadtare , Bing Xia , Guoliang Qing , Haiping Ke
发明人： Masayori Inouye , Sangita Phadtare , Bing Xia , Guoliang Qing , Haiping Ke
IPC分类号： G01N24/08 , C12N15/00 , C12N1/21 , C12P21/00
CPC分类号： C12N15/67 , C07K2299/00 , C12N15/70 , Y10T436/24
摘要： The present invention relates to a DNA molecule or vector and a host cell containing this DNA molecule or vector which can be used to produce a heterologous polypeptide under conditions that elicit a cold shock response in the host cell. The DNA molecule and vector include a nucleotide sequence encoding a heterologous polypeptide and a promoter and 5′-UTR from a cold shock inducible gene which directs its expression. In addition, an AT-rich sequence that enhances translation under cold shock inducible conditions is either present in the coding sequence of the heterologous polypeptide or in an additional element inserted between the coding sequence and the cold shock inducible promoter and 5′-UTR.
摘要翻译：本发明涉及含有该DNA分子或载体的DNA分子或载体和宿主细胞，其可以在宿主细胞中引起冷休克反应的条件下用于产生异源多肽。 DNA分子和载体包括编码异源多肽的核苷酸序列和引导其表达的冷休克诱导型基因的启动子和5'-UTR。此外，在冷休克诱导条件下增强翻译的富含AT的序列存在于异源多肽的编码序列中，或存在于插入编码序列和冷休克诱导型启动子和5'-UTR之间的另外的元件中。

39. 发明申请

US20090215120A1 The Use of Protein S Fusion for Protein Solubilization 审中-公开
标题翻译：蛋白S融合蛋白质溶解的应用
公开(公告)号：US20090215120A1
公开(公告)日：2009-08-27
申请号：US12293866
申请日：2007-03-20
申请人： Masayori Inouye , Takeshi Yoshida
发明人： Masayori Inouye , Takeshi Yoshida
IPC分类号： C12P21/00 , C12N15/00 , C07K14/00 , C07K1/00 , G01N33/68
CPC分类号： C07K14/195 , C07K2319/35
摘要： The invention provides vectors containing a multiple cloning site comprising a PrS tag or a PrS2 tag from Myxococcus xanthus. Methods are provided for enhancing solubility of a target protein using Protein S tagged target fusion proteins.
摘要翻译：本发明提供了含有多重克隆位点的载体，该多克隆位点包含来自黄粘球菌的PrS标签或PrS2标签。提供了使用蛋白S标记的靶融合蛋白提高靶蛋白的溶解度的方法。

40. 发明授权

US6162627A Methods of identifying inhibitors of sensor histidine kinases through rational drug design 失效
标题翻译：通过合理的药物设计鉴定传感器组氨酸激酶抑制剂的方法
公开(公告)号：US6162627A
公开(公告)日：2000-12-19
申请号：US158843
申请日：1998-09-23
申请人： Masayori Inouye , Heiyoung Park , Mitsuhiko Ikura
发明人： Masayori Inouye , Heiyoung Park , Mitsuhiko Ikura
IPC分类号： C12N9/12 , C07K1/00 , C12N1/20 , C12N5/00 , C12N15/00
CPC分类号： C12N9/1223 , C07K2319/00
摘要： The present invention provides N-terminal truncated transmembrane sensor histidine kinases that retain their ability to be autophosphorylated and/or their related histidine kinase activity. The N-terminal truncated transmembrane sensor histidine kinases are useful for obtaining detailed three-dimensional structural data of the catalytic portion of the protein. The three-dimensional structural data is included as part of the invention. In addition, the present invention provides methodology for related structure based rational drug design using the three-dimensional data. Nucleotide and amino acid sequences of the N-terminal truncated transmembrane sensor histidine kinases are also provided.
摘要翻译：本发明提供了保持其自身磷酸化能力和/或其相关组氨酸激酶活性的N-末端截短的跨膜传感器组氨酸激酶。 N-末端截短的跨膜传感器组氨酸激酶可用于获得蛋白质的催化部分的详细的三维结构数据。作为本发明的一部分包括三维结构数据。另外，本发明提供了使用三维数据的基于相关结构的合理药物设计方法。还提供了N-末端截短的跨膜传感器组氨酸激酶的核苷酸和氨基酸序列。

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