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31. 发明授权

US07666618B2 Lipolytic enzyme: uses thereof in the food industry 失效
标题翻译：脂肪分解酶：在食品工业中的应用
公开(公告)号：US07666618B2
公开(公告)日：2010-02-23
申请号：US11623607
申请日：2007-01-16
申请人： Andrei Miasnikov , Jørn Borch Søe , Jørn Dalgaard Mikkelsen , Mira Povelainen , Virve Pitkanen
发明人： Andrei Miasnikov , Jørn Borch Søe , Jørn Dalgaard Mikkelsen , Mira Povelainen , Virve Pitkanen
IPC分类号： C12Q1/34 , C12N9/16
CPC分类号： C12N9/1029 , A21D8/042 , A23J7/00 , C11B3/003 , C12N9/20 , C12P7/6418 , C12P7/6445 , C12P7/6481 , C12Y301/01003
摘要： The invention encompasses the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein the lipolytic enzyme hydrolyzes a glycolipid or a phospholipid or transferring an acyl group from a glycolipid or phospholipids to an acyl acceptor. The present invention also relates to a lipolytic enzyme that hydrolyzes at least a galactolipid or transfers an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Streptomyces species.
摘要翻译：本发明包括在各种方法和用途中使用可从以下属中的一种获得的脂肪分解酶：链霉菌，棒状杆菌和Thermobifida，其中所述脂肪分解酶水解糖脂或磷脂或将糖基从糖脂或磷脂转移至酰基受体。本发明还涉及一种脂解酶，其至少水解半乳糖脂或将酰基从半乳糖脂转移到一个或多个酰基受体底物，其中该酶可从链霉菌属物种获得。

32. 发明授权

US06929936B1 Composition comprising an enzyme having galactose oxidase activity and use thereof 失效
标题翻译：包含具有半乳糖氧化酶活性的酶的组合物及其用途
公开(公告)号：US06929936B1
公开(公告)日：2005-08-16
申请号：US09462911
申请日：1998-07-16
申请人： Xavier Rouau , Mette Schrøder , Jørn Borch Søe
发明人： Xavier Rouau , Mette Schrøder , Jørn Borch Søe
IPC分类号： A21D8/04 , A23L7/104 , A23L7/109 , C12N9/04 , A23L11/05
CPC分类号： C12Y101/03009 , A21D8/042 , A23L7/107 , A23L7/109 , C12Y302/01089
摘要： A dough and bread improving composition comprising an enzyme having galactose oxidase activity and an oxidizable substrate for this enzyme and/or an enzyme which is capable of converting a compound, e.g. a galactose-containing compound into a substrate for the enzyme having galactose oxidase activity.
摘要翻译：一种面团和面包改进组合物，其包含具有半乳糖氧化酶活性的酶和该酶的可氧化底物和/或能够转化化合物的酶，例如，含半乳糖的化合物转化成具有半乳糖氧化酶活性的酶的底物。

33. 发明授权

US08440435B2 Method for reducing 1,2-diglyceride content of an edible oil 失效
标题翻译：降低食用油1,2-二甘油酯含量的方法
公开(公告)号：US08440435B2
公开(公告)日：2013-05-14
申请号：US12492042
申请日：2009-06-25
申请人： Paul Wassell , Jørn Borch Søe , Jørn Dalgaard Mikkelsen , Anna Cecilie Jentoft Kristensen
发明人： Paul Wassell , Jørn Borch Søe , Jørn Dalgaard Mikkelsen , Anna Cecilie Jentoft Kristensen
IPC分类号： C12P7/64 , C12P21/06 , C12Q1/48 , C11N9/10 , C07H21/04
CPC分类号： C11C1/08 , C11C3/06 , C12N9/1029 , C12P7/6436 , C12P7/6445 , C12Y203/01
摘要： The present application provides a method of reducing and/or removing diglyceride from an edible oil, comprising admixing an edible oil with an acyl acceptor substrate and a diglyceride:glycerol acyltransferase, wherein the diglyceride:glycerol acyltransferase is characterized as an enzyme which in an edible oil is capable of transferring an acyl group from a diglyceride to glycerol. The diglyceride:glycerol acyltransferase can comprise the amino acid sequence motif GDSX. The present invention also relates to the use of a diglyceride:glycerol acyltransferase in the manufacture of an edible oil, for reducing and/or removing diglyceride from said edible oil and to the use of said enzyme in the manufacture of a foodstuff comprising an edible oil for improving the crystallization properties of said foodstuff.
摘要翻译：本申请提供了从食用油中还原和/或除去甘油二酯的方法，包括将食用油与酰基受体底物和甘油二酯：甘油酰基转移酶混合，其中所述甘油二酯：甘油酰基转移酶被表征为可食用的酶油能够将酰基从甘油二酯转移到甘油。甘油二酯：甘油酰基转移酶可以包含氨基酸序列基序GDSX。本发明还涉及甘油二酯：甘油酰基转移酶在制造食用油中的用途，用于从所述食用油中还原和/或除去甘油二酯，以及所述酶在制造包含食用油的食品中的用途用于改善所述食品的结晶性能。

34. 发明授权

US08030044B2 Lipid acyltransferases 有权
标题翻译：脂质酰基转移酶
公开(公告)号：US08030044B2
公开(公告)日：2011-10-04
申请号：US12040721
申请日：2008-02-29
申请人： Jørn Borch Søe , Jørn Dalgaard Mikkelson , Arno de Kreij
发明人： Jørn Borch Søe , Jørn Dalgaard Mikkelson , Arno de Kreij
IPC分类号： C12N9/10 , C07H21/04
CPC分类号： C12N9/1029 , A21D8/042 , A23L15/25 , A23L33/10 , A23L33/195 , C11B3/003 , C12P7/62 , C12P7/6436 , C12P7/6454 , C12P7/6481
摘要： The present invention relates to a method of producing a variant lipid acyltransferase enzyme by selecting a parent enzyme which is a lipid acyltransferase enzyme having the amino acid sequence motif GDSX, modifying one or more amino acids to produce a variant lipid acyltransferase, testing the variant lipid acyltransferase for activity on a galactolipid substrate, a phospholipid substrate and/or a triglyceride substrate, selecting a variant enzyme with an enhanced activity towards galactolipids compared with the parent enzyme, and/or preparing a quantity of the variant enzyme. In some embodiments, the variant lipid acyltransferase enzyme may include the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, X, Q, T N, M or S, and wherein the variant enzyme has one or more amino acid modifications compared with a parent sequence.
摘要翻译：本发明涉及通过选择具有氨基酸序列基序GDSX的脂质酰基转移酶的母体酶，修饰一个或多个氨基酸以产生变体脂质酰基转移酶来生产变体脂质酰基转移酶的方法，测试变体脂质用于在半乳糖脂底物，磷脂底物和/或甘油三酯底物上的活性的酰基转移酶，与母体酶相比，选择具有增强的对乳糖脂的活性的变体酶和/或制备一定量的变体酶。在一些实施方案中，变体脂质酰基转移酶可以包括氨基酸序列基序GDSX，其中X是以下氨基酸残基L，A，V，I，F，Y，X，Q，TN，M中的一个或多个或 S，并且其中所述变体酶与亲本序列相比具有一个或多个氨基酸修饰。

35. 发明申请

US20100215803A1 PROCESS 有权
标题翻译：处理
公开(公告)号：US20100215803A1
公开(公告)日：2010-08-26
申请号：US12706210
申请日：2010-02-16
申请人： Niels Erik Larsen , Jørn Borch Søe
发明人： Niels Erik Larsen , Jørn Borch Søe
IPC分类号： A23C9/12 , A23C9/123 , A23C3/02
CPC分类号： A21D8/042 , A23C19/0328 , A23C19/0684 , A23L15/25 , A23L29/06 , C12N9/1029 , C12N9/18 , C12N11/00 , C12Y301/01
摘要： The use of a lipid acyltransferase in the manufacture of UHT milk for improving the stability, particularly the long term stability, and/or improving the perceptible sensory difference and/or improving smell and/or taste and/or for reducing the cholesterol content and/or for eliminating or reducing creaming of the UHT milk for reducing the cholesterol content in the UHT milk. A method of producing UHT milk, wherein method comprises admixing a lipid acyltransferase and milk (including a step of processing the milk to make it a UHT milk). Preferably said lipid acyltranferase is a polypeptide having lipid acyltransferase activity which polypeptide is obtained by expression of the nucleotide sequence shown as SEQ ID No. 49 or a nucleotide sequence which as has 70% or more identity therewith; and/or is obtained by expression of a nucleic acid which hybridises under medium stringency conditions to a nucleic probe comprising the nucleotide sequence shown as SEQ ID No. 49; and/or is a polypeptide having lipid acyltranferase activity which polypeptide comprises the amino acid sequence shown as SEQ ID No. 68 or an amino acid sequence which as has 70% or more identity therewith.
摘要翻译：脂质酰基转移酶在制造UHT乳中用于改善稳定性，特别是长期稳定性和/或改善可察觉的感觉差异和/或改善气味和/或味道和/或降低胆固醇含量和/ 或用于消除或减少UHT牛奶的奶油以降低UHT牛奶中的胆固醇含量。一种生产UHT奶的方法，其中方法包括混合脂质酰基转移酶和乳（包括加工牛奶以使其成为UHT牛奶的步骤）。优选地，所述脂质酰基转移酶是具有脂质酰基转移酶活性的多肽，其多肽通过表达如SEQ ID No.49所示的核苷酸序列或与其同一性具有70％或更多同一性的核苷酸序列获得; 和/或通过在中等严格条件下与包含SEQ ID No.49所示的核苷酸序列的核酸探针杂交的核酸的表达获得; 和/或是具有脂质酰基转移酶活性的多肽，该多肽包含SEQ ID No.68所示的氨基酸序列或与其同一性具有70％以上的氨基酸序列。

36. 发明授权

US07781001B2 Foodstuff 有权
标题翻译：食品
公开(公告)号：US07781001B2
公开(公告)日：2010-08-24
申请号：US10409391
申请日：2003-04-08
申请人： Jørn Borch Søe
发明人： Jørn Borch Søe
IPC分类号： A23L1/23
CPC分类号： A23D7/011 , A21D2/16 , A21D13/38 , A23D7/013 , A23D7/015 , A23G1/423 , A23G3/366 , A23G3/40 , A23G9/327 , A23G9/363 , A23L9/22 , A23L29/06 , C11C3/06 , C12P7/6454 , C12Y301/01003
摘要： There is provided use of a conversion agent to prepare from a food material a foodstuff comprising at least one functional ingredient, wherein the at least one functional ingredient has been generated from at least one constituent of the food material by the conversion agent.
摘要翻译：提供了使用转化剂从食品制备包含至少一种功能成分的食品，其中至少一种功能成分是由转化剂从食品材料的至少一种成分产生的。

37. 发明申请

US20120231116A1 METHOD 审中-公开
标题翻译：方法
公开(公告)号：US20120231116A1
公开(公告)日：2012-09-13
申请号：US13510499
申请日：2010-11-08
申请人： Niels Erik Larsen , Jørn Borch Søe
发明人： Niels Erik Larsen , Jørn Borch Søe
IPC分类号： A23C9/18
CPC分类号： A23C9/1216 , C07K14/71 , C12Y203/01043
摘要： A method of producing powder milk, said method comprising: (a) contacting milk or a fraction thereof with a lipid acyltransferase enzyme; and (b) drying the enzyme treated milk to produce powder milk; is disclosed. Powder milk products produced by the method and use of a lipid acyltransferase in the manufacture of powder milk for improving the rehydration properties, the perceptible sensory difference (smell and/or taste) and the flowability of the powder milk, and for reducing the cholesterol content and/or the free fatty acid content of the powder milk, and for reducing fouling of the equipment used in the manufacture of the powder milk, are also disclosed.
摘要翻译：一种生产粉末乳的方法，所述方法包括：（a）使乳或其一部分与脂质酰基转移酶接触; 和（b）干燥酶处理的牛奶以产生粉末奶; 被披露。通过脂质酰基转移酶的制造方法生产的粉末乳制品，用于制造粉末乳，用于改善补液性能，可感知的感觉差异（气味和/或味道）和粉末奶的流动性以及降低胆固醇含量和/或粉末奶的游离脂肪酸含量，以及用于减少用于制造粉末乳的设备的结垢。

38. 发明授权

US08163315B2 Foodstuff 失效
标题翻译：食品
公开(公告)号：US08163315B2
公开(公告)日：2012-04-24
申请号：US13085567
申请日：2011-04-13
申请人： Jørn Borch Søe
发明人： Jørn Borch Søe
IPC分类号： A23L1/34
CPC分类号： A23D7/011 , A21D2/16 , A21D13/38 , A23D7/013 , A23D7/015 , A23G1/423 , A23G3/366 , A23G3/40 , A23G9/327 , A23G9/363 , A23L9/22 , A23L29/06 , C11C3/06 , C12P7/6454 , C12Y301/01003
摘要： There is provided use of a conversion agent to prepare from a food material a foodstuff comprising at least one functional ingredient, wherein the at least one functional ingredient has been generated from at least one constituent of the food material by the conversion agent.
摘要翻译：提供了使用转化剂从食品制备包含至少一种功能成分的食品，其中至少一种功能成分是由转化剂从食品材料的至少一种成分产生的。

39. 发明授权

US08012732B2 Fungal lypolytic and amylase enzyme composition and methods using the same 有权
标题翻译：真菌溶血酶和淀粉酶组成及使用方法
公开(公告)号：US08012732B2
公开(公告)日：2011-09-06
申请号：US12552572
申请日：2009-09-02
申请人： Janne Brunstedt , Jørn Dalgaard Mikkelsen , Henrik Pedersen , Jørn Borch Søe
发明人： Janne Brunstedt , Jørn Dalgaard Mikkelsen , Henrik Pedersen , Jørn Borch Søe
IPC分类号： C12P1/00 , C12N9/00 , C12N9/20 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： A21D8/042 , A23C19/0328 , A23D7/02 , A23K20/189 , A23K50/75 , A23L7/107 , A23L7/109 , A23L7/111 , A23L27/60 , A23L29/06 , C11B3/003 , C12N9/18 , C12P7/62 , C12P19/44 , C12Y301/01
摘要： A fungal wild-type lipolytic enzyme having a higher ratio of activity on polar lipids compared with triglycerides, wherein the enzyme preferably has a phospholipid:triglyceride activity ratio of at least 4. Preferably, the lipolytic enzyme according to the present invention has a glycolipid:triglyceride hydrolyzing activity ratio of at least 1.5. In one embodiment, the fungal lipolytic enzyme according to the present invention comprises an amino acid sequence as shown in SEQ ID NO: 1 or SEQ ID No. 2 or SEQ ID No. 4 or SEQ ID No. 6 or an amino acid sequence which has at least 90% identity thereto. The present invention further encompasses a nucleic acid encoding a fungal lipolytic enzyme, which nucleic acid is selected from the group consisting of: (a) a nucleic acid comprising a nucleotide shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7; (b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7 by the degeneration of the genetic code; and (c) nucleic acid comprising a nucleotide sequence which has at least 90% identity with the nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7.
摘要翻译：与甘油三酯相比，极性脂质活性比例更高的真菌野生型脂肪分解酶，其中所述酶优选具有至少为4的磷脂：甘油三酯活性比。优选地，根据本发明的脂肪分解酶具有糖脂：甘油三酯水解活性比至少为1.5。在一个实施方案中，根据本发明的真菌脂肪分解酶包含如SEQ ID NO：1或SEQ ID No.2或SEQ ID No.4或SEQ ID No.6所示的氨基酸序列或氨基酸序列，与其具有至少90％的同一性。本发明还包括编码真菌脂肪分解酶的核酸，该核酸选自：（a）包含SEQ ID No.3，SEQ ID No.5或SEQ ID No.5所示核苷酸的核酸第7号（b）通过遗传密码的退化与SEQ ID No.3，SEQ ID No.5或SEQ ID No.7的核苷酸序列有关的核酸; 和（c）核酸，其包含与SEQ ID No.3，SEQ ID No.5或SEQ ID No.7所示的核苷酸序列具有至少90％同一性的核苷酸序列。

40. 发明授权

US09228211B2 Process of water degumming an edible oil 有权
标题翻译：水脱胶食用油的工艺
公开(公告)号：US09228211B2
公开(公告)日：2016-01-05
申请号：US12809901
申请日：2008-12-11
申请人： Jørn Borch Søe , Anne Victoria Brown
发明人： Jørn Borch Søe , Anne Victoria Brown
IPC分类号： C12P7/64 , C12N9/10 , C12N9/20 , A23D7/00 , C07H21/04 , C11B3/00
CPC分类号： C12P7/6445 , C11B3/003 , C12Y203/01043 , C12Y301/04003
摘要： A process of water degumming an edible oil (preferably a crude edible oil) comprising the steps of: a) admixing approximately 0.1-5% w/w water with an edible oil (preferably a crude edible oil) and a lipid acyltransferase, b) agitating the admixture for between about 10 minutes and 180 minutes at about 45 to about 900 C, and c) separating the oil phase and the gum phase. Preferably said lipid acyltransferase is a polypeptide having lipid acyltransferase activity which polypeptide is obtained by expression of the nucleotide sequence shown as SEQ ID No. 49 or a nucleotide sequence which as has 70% or more identity therewith; and/or is obtained by expression of a nucleic acid which hybridizes under medium stringency conditions to a nucleic probe comprising the nucleotide sequence shown as SEQ ID No. 49; and/or is a polypeptide having lipid acyltransferase activity which polypeptide comprises the amino acid sequence shown as SEQ ID No. 68 or an amino acid sequence which as has 70% or more identity therewith. In one embodiment the lipid acyltransferase is preferably used in combination with a phospholipase C enzyme. A process for modifying the gum phase of a degummed oil using a lipid acyltransferase is also taught herein.
摘要翻译：一种将食用油（优选粗制食用油）脱水的方法，包括以下步骤：a）将约0.1-5％w / w的水与食用油（优选粗制食用油）和脂质酰基转移酶混合，b）在约45至约900℃搅拌混合物约10分钟至180分钟，和c）分离油相和胶相。优选地，所述脂质酰基转移酶是具有脂质酰基转移酶活性的多肽，其多肽通过表达SEQ ID No.49所示的核苷酸序列或与其同一性具有70％以上的核苷酸序列而获得; 和/或通过在中等严格条件下与包含如SEQ ID No.49所示的核苷酸序列的核酸探针杂交的核酸的表达获得; 和/或具有脂质酰基转移酶活性的多肽，该多肽包含SEQ ID No.68所示的氨基酸序列或与其同一性具有70％以上的氨基酸序列。在一个实施方案中，脂质酰基转移酶优选与磷脂酶C酶组合使用。本文还教导了使用脂质酰基转移酶改性脱胶油的胶相的方法。

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