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31. 发明申请

US20110217735A1 COMPOSITIONS AND METHODS FOR REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION (RT-PCR) 有权
标题翻译：反向转录酶 - 聚合酶链反应的组合物和方法（RT-PCR）
公开(公告)号：US20110217735A1
公开(公告)日：2011-09-08
申请号：US12981240
申请日：2010-12-29
申请人： Jun E. Lee , Ayoub Rashtchian
发明人： Jun E. Lee , Ayoub Rashtchian
IPC分类号： C12P19/34 , C12N9/12
CPC分类号： C12P19/34 , C12N9/1276 , C12N15/1096 , C12Q1/6846 , C12Q2527/149 , C12Q2527/125 , C12Q2521/107
摘要： The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.
摘要翻译：本发明涉及通过逆转录酶 - 聚合酶链反应（RT-PCR）扩增核酸分子的组合物和方法。具体地，本发明提供了使用逆转录酶和热稳定DNA聚合酶与含硫分子或含乙酸酯分子结合的简化的一步或两步RT-PCR方法扩增核酸分子的组合物和方法（或这种含硫分子和含乙酸酯的分子的组合）和任选的牛血清白蛋白。因此，本发明促进了核酸分子的快速和有效的扩增以及RNA分子的检测和定量。本发明也可用于快速生产和扩增可用于各种工业，医学和法医目的的cDNA。

32. 发明授权

US07638612B2 Compositions and methods for cDNA synthesis 有权
标题翻译： cDNA合成的组合物和方法
公开(公告)号：US07638612B2
公开(公告)日：2009-12-29
申请号：US10653416
申请日：2003-09-03
申请人： Ayoub Rashtchian , David M. Schuster
发明人： Ayoub Rashtchian , David M. Schuster
IPC分类号： C07H21/00 , C12Q1/68 , C12P19/34 , C07K14/00
CPC分类号： C12P19/34 , C12N15/1096 , C12Q1/6846 , C12Q2527/143 , C12Q2525/179 , C12Q2521/107
摘要： Methods for making cDNA molecules, for amplification of RNA by PCR and for preparation of cDNA libraries are provided. Kits for making cDNA molecules also are provided. Compositions are also provided comprising mixtures of reagents, including reverse transcriptases, buffers, cofactors and other components, suitable for immediate use in conversion of RNA into cDNA and RT PCR without dilution or addition of further components. These compositions are useful, alone or in the form of kits, for cDNA synthesis or nucleic acid amplification (e.g., by the Polymerase Chain Reaction) or for any procedure utilizing reverse transcriptases in a variety of research, medical, diagnostic, forensic and agricultural applications.
摘要翻译：提供了制备cDNA分子，通过PCR扩增RNA和制备cDNA文库的方法。还提供了制备cDNA分子的试剂盒。还提供组合物，其包括试剂的混合物，包括逆转录酶，缓冲液，辅因子和其它组分，适于立即用于将RNA转化成cDNA和RT PCR，而不进行稀释或添加其它组分。这些组合物可单独或以试剂盒的形式用于cDNA合成或核酸扩增（例如，通过聚合酶链式反应）或在各种研究，医学，诊断，法医和农业应用中使用逆转录酶的任何方法。

33. 发明授权

US07501252B2 Use of predetermined nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules 失效
公开(公告)号：US07501252B2
公开(公告)日：2009-03-10
申请号：US11768865
申请日：2007-06-26
申请人： David M. Schuster , Ayoub Rashtchian
发明人： David M. Schuster , Ayoub Rashtchian
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/6844 , C12Q1/6832 , C12Q1/6853 , C12Q1/686 , C12Q1/6862 , C12Q2600/156 , C12Q2527/107 , C12Q2525/101 , C12Q2521/107
摘要： The present invention provides improved methods for amplifying a nucleic acid molecule. More specifically, the invention provides methods for nucleic acid amplification which use primers having equivalent priming efficiency.

34. 发明授权

US07238500B2 Use of predetermined nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules 失效
标题翻译：在核酸分子扩增中使用具有改变的碱基配对特征的预定核苷酸
公开(公告)号：US07238500B2
公开(公告)日：2007-07-03
申请号：US11410976
申请日：2006-04-26
申请人： David M. Schuster , Ayoub Rashtchian
发明人： David M. Schuster , Ayoub Rashtchian
IPC分类号： C12P19/34 , C07H21/04
CPC分类号： C12Q1/6844 , C12Q1/6832 , C12Q1/6853 , C12Q1/686 , C12Q1/6862 , C12Q2600/156 , C12Q2527/107 , C12Q2525/101 , C12Q2521/107
摘要： The present invention provides improved methods for amplifying a nucleic acid molecule. More specifically, the invention provides methods for nucleic acid amplification which use primers having equivalent priming efficiency.
摘要翻译：本发明提供了用于扩增核酸分子的改进方法。更具体地，本发明提供了使用具有等效启动效率的引物的核酸扩增方法。

35. 发明申请

US20060068436A1 Compositions and methods for cDNA synthesis 有权
公开(公告)号：US20060068436A1
公开(公告)日：2006-03-30
申请号：US11237922
申请日：2005-09-29
申请人： Ayoub Rashtchian , David Schuster
发明人： Ayoub Rashtchian , David Schuster
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12P19/34 , C12N15/1096 , C12Q1/6846 , C12Q2527/143 , C12Q2525/179 , C12Q2521/107
摘要： Methods for making cDNA molecules, for amplification of RNA by PCR and for preparation of cDNA libraries are provided. Kits for making cDNA molecules also are provided. Compositions are also provided comprising mixtures of reagents, including reverse transcriptases, buffers, cofactors and other components, suitable for immediate use in conversion of RNA into cDNA and RT PCR without dilution or addition of further components. These compositions are useful, alone or in the form of kits, for cDNA synthesis or nucleic acid amplification (e.g., by the Polymerase Chain Reaction) or for any procedure utilizing reverse transcriptases in a variety of research, medical, diagnostic, forensic and agricultural applications.

36. 发明授权

US06933121B2 Use of predetermined nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules 失效
标题翻译：在核酸分子扩增中使用具有改变的碱基配对特征的预定核苷酸
公开(公告)号：US06933121B2
公开(公告)日：2005-08-23
申请号：US10396315
申请日：2003-03-26
申请人： David M. Schuster , Ayoub Rashtchian
发明人： David M. Schuster , Ayoub Rashtchian
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6844 , C12Q1/6832 , C12Q1/6853 , C12Q1/686 , C12Q1/6862 , C12Q2600/156 , C12Q2527/107 , C12Q2525/101 , C12Q2521/107
摘要： The present invention provides improved methods for amplifying a nucleic acid molecule. More specifically, the invention provides methods for nucleic acid amplification which use primers having equivalent priming efficiency.
摘要翻译：本发明提供了用于扩增核酸分子的改进方法。更具体地，本发明提供了使用具有等效启动效率的引物的核酸扩增方法。

37. 发明授权

US06610490B2 Compositions, kits and uses of nucleotides having altered base pairing characteristics 失效
标题翻译：具有改变的碱基配对特征的核苷酸的组合物，试剂盒和用途
公开(公告)号：US06610490B2
公开(公告)日：2003-08-26
申请号：US09893345
申请日：2001-06-28
申请人： David M. Schuster , Ayoub Rashtchian
发明人： David M. Schuster , Ayoub Rashtchian
IPC分类号： C12Q168
CPC分类号： C12Q1/6844 , C12Q1/6832 , C12Q1/6853 , C12Q1/686 , C12Q1/6862 , C12Q2600/156 , C12Q2527/107 , C12Q2525/101 , C12Q2521/107
摘要： The present invention provides improved methods, compositions and kits for amplifying a nucleic acid molecule. Specifically, the invention involves replacing at least one nucleotide of an oligonucleotide with nucleotide residue having altered base-pairing characteristics, such as inosine, hypoxanthine, xanthine, and methylated nucleotide derivatives, so as to more equalize the efficiency with which that oligonucleotide and a second oligonucleotide hybridize to a target molecule, and then amplifying the target molecule using, for example, the polymerase chain reaction. Improved amplification results from the improvement in the relative hybridization efficiencies.
摘要翻译：本发明提供用于扩增核酸分子的改进的方法，组合物和试剂盒。具体地说，本发明涉及用寡核苷酸替换具有改变的碱基配对特性的核苷酸残基的寡核苷酸的至少一个核苷酸，例如肌苷，次黄嘌呤，黄嘌呤和甲基化核苷酸衍生物，以便更均衡该寡核苷酸和第二寡核苷酸与靶分子杂交，然后使用例如聚合酶链反应扩增靶分子。改进的扩增来自相对杂交效率的提高。

38. 发明授权

US5792854A Detection of salmonella 失效
标题翻译：检测沙门氏菌
公开(公告)号：US5792854A
公开(公告)日：1998-08-11
申请号：US465987
申请日：1995-06-06
申请人： David J. Lane , Ayoub Rashtchian , Kyriaki Parodos
发明人： David J. Lane , Ayoub Rashtchian , Kyriaki Parodos
IPC分类号： C12N15/09 , C12Q1/10 , C12Q1/68 , C12R1/42 , C07H21/04 , C12N15/10 , C12N15/31
CPC分类号： C12Q1/689 , C12Q1/6813
摘要： Nucleic acid fragments capable of hybridizing to rRNA of a Salmonella species and not capable of hybridizing to rRNA of Escherichia coli.
摘要翻译：能够与沙门氏菌属物种的rRNA杂交并且不能与大肠杆菌的rRNA杂交的核酸片段。

39. 发明授权

US5334515A Method for altering a nucleotide sequence 失效
标题翻译：改变核苷酸序列的方法
公开(公告)号：US5334515A
公开(公告)日：1994-08-02
申请号：US38071
申请日：1993-03-29
申请人： Ayoub Rashtchian , David M. Schuster , George W. Buchman, III
发明人： Ayoub Rashtchian , David M. Schuster , George W. Buchman, III
IPC分类号： C12N15/10 , C12Q1/68 , C07H21/04 , C12N15/11 , C12P19/34
CPC分类号： C12Q1/6853 , C12N15/1096 , C12Q1/6865
摘要： A method and kit, employing exo-sample nucleotides such as deoxyuridine, capable of altering the nucleic acid sequence present at the 3' or 5' end of a DNA or RNA molecule is provided. The method and kit can be used to achieve the selective amplification of nucleic acid molecules.
摘要翻译：提供了一种使用能够改变存在于DNA或RNA分子的3'或5'末端的核酸序列的外切核苷酸例如脱氧尿苷的方法和试剂盒。该方法和试剂盒可用于实现核酸分子的选择性扩增。

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