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31. 发明授权

US5254470A Alkaline protease, alkaline protease gene, recombinant DNA, DNA fragment for the expression of gene, and process for the production of alkaline protease 失效
标题翻译：碱性蛋白酶，碱性蛋白酶基因，重组DNA，用于表达基因的DNA片段，以及生产碱性蛋白酶的方法
公开(公告)号：US5254470A
公开(公告)日：1993-10-19
申请号：US672304
申请日：1991-03-20
申请人： Seiji Murakami , Hiroki Tatsumi , Yoshihiro Ogawa , Eiichi Nakano , Hiroshi Motai , Shigetoshi Sugio , Atsushi Masaki , Yutaka Ishida , Kohji Murakami , Haruhide Kawabe , Hirofumi Arimura
发明人： Seiji Murakami , Hiroki Tatsumi , Yoshihiro Ogawa , Eiichi Nakano , Hiroshi Motai , Shigetoshi Sugio , Atsushi Masaki , Yutaka Ishida , Kohji Murakami , Haruhide Kawabe , Hirofumi Arimura
IPC分类号： C12N15/09 , C12N9/62 , C12N15/51 , C12R1/69
CPC分类号： C12N9/62
摘要： Alkaline protease which has leucine or isoleucine in the place of valine at the amino acid number 40 of wild type alkaline protease, a gene encoding the amino acid sequence of alkaline protease which has the substitution as described above, a recombinant DNA comprising said gene, a method of producing the above described alkaline protease, and a DNA fragment used for the expression of a gene.
摘要翻译：在野生型碱性蛋白酶的氨基酸数40处含有亮氨酸或异亮氨酸代替缬氨酸的碱性蛋白酶，编码如上所述取代的碱性蛋白酶的氨基酸序列的基因，包含所述基因的重组DNA，制备上述碱性蛋白酶的方法和用于表达基因的DNA片段。

32. 发明授权

US5219737A Mutant luciferase of a firefly, mutant luciferase genes, recombinant DNAS containing the genes and a method of producing mutant luciferase 失效
标题翻译：一种脆弱的突变型基因，含有该基因的重组DNA和一种产生突变型突变体的方法
公开(公告)号：US5219737A
公开(公告)日：1993-06-15
申请号：US675211
申请日：1991-03-26
申请人： Naoki Kajiyama , Eiichi Nakano
发明人： Naoki Kajiyama , Eiichi Nakano
IPC分类号： C12N9/02 , C12N15/53 , C12Q1/68
CPC分类号： C12N9/0069 , C12Q1/6897 , C12Y113/12007
摘要： The present invention provides industrially useful luciferase. Mutant luciferase of the invention is produced by culturing a microorganism belonging to the genus Escherichia which harbors a recombinant DNA containing the mutant luciferase gene of a firefly. Mutant luciferase can produce red, orange or green color of light which can not be produced by wild type luciferase. Mutant luciferase can be used to measure ATP accurately in a colored solution such as red (e.g., blood), orange, or green in which wild-type luciferase has not provided reliable results.

33. 发明授权

US4968613A Luciferase gene and novel recombinant DNA as well as a method of producing luciferase 失效
标题翻译：荧光素酶基因和新型重组DNA以及荧光素酶的生产方法
公开(公告)号：US4968613A
公开(公告)日：1990-11-06
申请号：US224445
申请日：1988-07-26
申请人： Tsutomu Masuda , Hiroki Tatsumi , Eiichi Nakano
发明人： Tsutomu Masuda , Hiroki Tatsumi , Eiichi Nakano
IPC分类号： C12N9/02
CPC分类号： C12N9/0069 , C12Y113/12007
摘要： A luciferase gene isolated from Luciola cruciata (Japanese firefly) coding for an amino acid sequence shown in FIG. 4 and a novel recombinant DNA characterized by incorporating a gene coding for luciferase into a vector DNA are disclosed. There is also disclosed a method of producing luciferase which comprises culturing in a medium a microorganism containing a recombinant DNA having inserted a gene coding for luciferase in a vector DNA and belonging to the genus Escherichia capable of producing luciferase and collecting luciferase from the culture.

34. 发明授权

US4834766A Method for preventing scale adhesion 失效
标题翻译：防止垢附着的方法
公开(公告)号：US4834766A
公开(公告)日：1989-05-30
申请号：US225525
申请日：1988-07-28
申请人： Yoshiteru Sando , Hiroshi Ishidoshiro , Eiichi Nakano
发明人： Yoshiteru Sando , Hiroshi Ishidoshiro , Eiichi Nakano
IPC分类号： D06B3/10 , D06B3/12 , D06B21/00 , D06B23/16
CPC分类号： D06B23/16
摘要： A method for preventing the adhesion of scale on the sealing mechanism of a high pressure steamer in a process of continuous scouring of a long cloth therein, which comprises washing the cloth to be subjected to scouring with the use of weak acidic water with a pH of 5-6 outside of the steamer body, passing the thus washed cloth through the sealing mechanism of the steamer body, and then applying a scouring agent solution to the cloth in the interior of the steamer body. A durable sealing mechanism can be offered by preventing the damage of the sealing mechanism.
摘要翻译：一种在连续冲刷其中的长布的过程中防止水垢附着在高压蒸笼的密封机构上的方法，其中包括使用弱酸性水洗涤待冲刷的布，pH为 5-6在蒸笼体外部，将如此洗涤的布穿过蒸笼体的密封机构，然后将擦洗剂溶液施加到蒸笼体内部的布上。可以通过防止密封机构的损坏提供耐用的密封机构。

35. 发明授权

US4348477A Method for preparing a recombinant DNA phage 失效
标题翻译：制备重组DNA噬菌体的方法
公开(公告)号：US4348477A
公开(公告)日：1982-09-07
申请号：US87166
申请日：1979-10-22
申请人： Eiichi Nakano , Tsutomu Masuda , Narimasa Saito , Danji Fukushima
发明人： Eiichi Nakano , Tsutomu Masuda , Narimasa Saito , Danji Fukushima
IPC分类号： C12N15/09 , C12N15/00 , C12P19/34 , C12R1/91
CPC分类号： C12N15/00
摘要： A method for preparing a novel recombinant DNA, which comprises (1) cleaving with an endonuclease a phage DNA having an endonuclease-sensitive region not in the DNA segment participating in temperate phage DNA replication and integration of DNA into a host chromosome but in other DNA segments, a temperate phage DNA having an endonuclease-sensitive region in the DNA segment carrying genetic information for the production of coat protein, and a DNA carrying the intended genetic information, (2) mixing together all fragments produced by said cleaving, (3) adding DNA ligase to the mixture, and (4) recovering from the resulting mixture a phage DNA having its coat protein producing ability deleted by the replacement of the DNA segment carrying genetic information for coat protein production with a DNA fragment carrying the intended genetic information.
摘要翻译：一种制备新型重组DNA的方法，其包括（1）用核酸内切酶切割具有不在DNA片段中的内切核酸酶敏感区域的噬菌体DNA，其参与温和噬菌体DNA复制并将DNA整合到宿主染色体中，但在其他DNA （2）将由所述切割产生的所有片段混合在一起，（3）将所述切割产生的所有片段混合在一起，其中所述DNA片段具有用于产生外壳蛋白的遗传信息的DNA段中具有内切核酸酶敏感区的温带噬菌体DNA，向混合物中加入DNA连接酶，（4）从所得混合物中回收具有外壳蛋白产生能力的噬菌体DNA，通过用携带预期遗传信息的DNA片段替代携带用于外壳蛋白生产的遗传信息的DNA片段而缺失。

36. 发明授权

US4066541A Process for treating waste water containing polyvinyl alcohol 失效
标题翻译：用于处理含有聚乙烯醇的废水的方法
公开(公告)号：US4066541A
公开(公告)日：1978-01-03
申请号：US688069
申请日：1976-05-19
申请人： Yoshikazu Sando , Hiroshi Ishidoshiro , Eiichi Nakano
发明人： Yoshikazu Sando , Hiroshi Ishidoshiro , Eiichi Nakano
IPC分类号： C08L29/00 , C02F1/52 , C02F1/58 , C02F9/00 , C08K3/38 , C08L29/02 , D06L1/14 , D06M15/333 , B01D5/00 , B01D11/00 , B01D17/00
CPC分类号： D06M15/333 , C08K3/38 , C02F2101/34 , C02F2103/38
摘要： A process for treating waste water containing polyvinyl alcohol (designated hereinafter as P.V.A.) comprising separation of the P.V.A. in the form of a compound with boric acid which is produced at pH 8 to 10 by a reaction with boric acid or borax added in the presence of an inorganic salt such as sodium sulfate, and using thus regenerated liquid repeatedly, and a process of utilizing waste containing P.V.A. for desizing textile.

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