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31. 发明授权

US07897758B2 Activators for oligonucleotide and phosphoramidite synthesis 有权
标题翻译：用于寡核苷酸和亚磷酰胺合成的活化剂
公开(公告)号：US07897758B2
公开(公告)日：2011-03-01
申请号：US11380227
申请日：2006-04-26
申请人： Andreas Wolter , Michael Leuck
发明人： Andreas Wolter , Michael Leuck
IPC分类号： C07H21/02 , C07H21/04
CPC分类号： C07H21/02 , C07H21/04
摘要： The present invention discloses novel methods for the synthesis of oligonucleotides and nucleoside phosphoramidites. The methods are based on employing aryl-substituted 5-phenyl-1H-tetrazoles with perfluoroalkyl groups on the aromatic ring as activators. In one aspect the novel activators are used in the synthesis of oligonucleotides via the phosphoramidite approach. In this aspect the activators are highly efficient and can be applied with very short coupling times. In a further aspect, the activators of the invention are used in the synthesis of phosphoramidites through the reaction of nucleosides comprising a free hydroxyl group with phosphitylating agents. In this aspect the activators provide very pure phosphoramidites under mild conditions. The activators of the invention are further characterized by being highly soluble, non-hygroscopic and non-hazardous.
摘要翻译：本发明公开了合成寡核苷酸和核苷亚磷酰胺的新方法。该方法基于在芳环上使用具有全氟烷基的芳基取代的5-苯基-1H-四唑作为活化剂。在一个方面，新的活化剂用于通过亚磷酰胺方法合成寡核苷酸。在这方面，活化剂是高效的并且可以以非常短的偶联时间施用。在另一方面，本发明的活化剂用于通过包含游离羟基的核苷与磷酸化剂的反应来合成亚磷酰胺。在这方面，活化剂在温和条件下提供非常纯的亚磷酰胺。本发明的活化剂的特征还在于高溶解性，非吸湿性和非危险性。

32. 发明授权

US07098326B2 Methods for the integrated synthesis and purification of oligonucleotides 有权
公开(公告)号：US07098326B2
公开(公告)日：2006-08-29
申请号：US10349195
申请日：2003-01-22
申请人： Wolfgang Pieken , Andreas Wolter , Michael Leuck
发明人： Wolfgang Pieken , Andreas Wolter , Michael Leuck
IPC分类号： C07H21/00 , C07H21/02 , C07H21/04
CPC分类号： C07H21/00 , C07H21/04
摘要： The present invention discloses novel methods for the integrated synthesis and purification of oligonucleotides. The methods employ novel capping reagents carrying two functional groups. The first functional group provides for a smooth and efficient capping process and incorporates the second functional group into contaminant oligonucleotides during solid phase oligonucleotide synthesis. The second functional group functions as a chemical purification handle in the trapping of truncated oligonucleotides (failure sequences) on a solid support. The trapping process creates covalent bonds between the solid support and the truncated oligonucleotides and therefore allows the removal of the truncated sequences from the desired full length oligonucleotide product by filtration. The chemical trapping process employed in this invention is based on cycloaddition reactions, particularly Diels-Alder reactions between the truncated oligonucleotides and the trapping agent. The invention includes novel solid support compositions that carry covalently attached Diels-Alder reaction components.

33. 发明授权

US06902900B2 Nucleic acid probes and methods to detect and/or quantify nucleic acid analytes 失效
标题翻译：核酸探针和检测和/或定量核酸分析物的方法
公开(公告)号：US06902900B2
公开(公告)日：2005-06-07
申请号：US10278047
申请日：2002-10-21
申请人： Martin Davies , Ian Bruce , Andreas Wolter
发明人： Martin Davies , Ian Bruce , Andreas Wolter
IPC分类号： C07H21/00 , C12Q1/68 , C07H21/02 , C07H21/04 , C09B39/00
CPC分类号： C12Q1/6818 , C07H21/00 , C12Q1/686 , C12Q2565/107 , C12Q2565/1015 , C12Q2563/173 , C12Q2525/313 , C12Q2527/119 , C12Q2525/113 , C12Q2561/113
摘要： The invention comprises novel methods and strategies to detect and/or quantify nucleic acid analytes. The methods involve nucleic acid probes with covalently conjugated dyes, which are attached either at adjacent nucleotides or at the same nucleotide of the probe and novel linker molecules to attach the dyes to the probes. The nucleic acid probes generate a fluorescent signal upon hybridization to complementary nucleic acids based on the interaction of one of the attached dyes, which is either an intercalator or a DNA groove binder, with the formed double stranded DNA. The methods can be applied to a variety of applications including homogeneous assays, real-time PCR monitoring, transcription assays, expression analysis on nucleic acid microarrays and other microarray applications such as genotyping (SNP analysis). The methods further include pH-sensitive nucleic acid probes that provide switchable fluorescence signals that are triggered by a change in the pH of the medium.
摘要翻译：本发明包括检测和/或定量核酸分析物的新方法和策略。该方法包括具有共价偶联染料的核酸探针，其连接在探针的相邻核苷酸或相同核苷酸处，并且连接分子以将染料附着到探针上。基于与形成的双链DNA的嵌入剂或DNA沟槽结合剂之一连接的染料之间的相互作用，核酸探针在与互补核酸杂交时产生荧光信号。该方法可以应用于各种应用，包括均一测定，实时PCR监测，转录测定，核酸微阵列和其他微阵列应用如基因分型（SNP分析）的表达分析。所述方法还包括pH敏感的核酸探针，其提供由介质pH的变化触发的可切换荧光信号。

34. 发明授权

US4655952A Detergent and method for producing the same 失效
标题翻译：洗涤剂及其制造方法
公开(公告)号：US4655952A
公开(公告)日：1987-04-07
申请号：US705680
申请日：1985-02-26
申请人： Otto Mesmer , Wolfgang Polligkeit , Ernst-Uwe Schiffer , Wolfgang Troger , Andreas Wolter
发明人： Otto Mesmer , Wolfgang Polligkeit , Ernst-Uwe Schiffer , Wolfgang Troger , Andreas Wolter
IPC分类号： C11D3/00 , C11D3/37 , C11D17/04 , C08G12/12 , C08J9/40 , C09K3/22
CPC分类号： C11D3/3703 , C11D17/049 , C11D3/0031
摘要： The invention is directed to a detergent and a method of producing the same. The detergent is for textile surfaces and especially for textile floor coverings. The detergent includes a pulverized, porous carrier including a foamed, plastified urea-formaldehyde resin foam. The carrier material is enriched with detergent so that the finished product with respect to the carrier material has a weight per unit volume of 60 kg/m.sup.3 and an apparent density of 50 to 150 grams/liter; the granular size of the carrier material is between 0.01 and 12 mm; the surfactant containing water, which adheres to the carrier material, is stored in the carrier material in a completely homogeneous manner in a share of maximally 80% by weight referred to the weight of the carrier material; and, the detergent is produced from the mixture of the carrier material with a highly concentrated aqueous cleaning solution. The method includes the steps of foaming the plastified urea-formaldehyde resin foam; and, combining the free formaldehyde present during the manufacturing process prior to and/or during the foaming of the plastified urea-formaldehyde resin foam by adding formaldehyde binding substances and stabilizing substances to at least one of the solutions selected from the group consisting of the foaming agent solution and the resin solution.
摘要翻译：本发明涉及一种洗涤剂及其制造方法。洗涤剂用于织物表面，特别是用于纺织品地板覆盖物。洗涤剂包括粉碎的多孔载体，其包括发泡的，塑化的脲 - 甲醛树脂泡沫。载体材料富含洗涤剂，使成品相对于载体材料的每单位体积的重量为60kg / m 3，表观密度为50至150克/升; 载体材料的粒度为0.01〜12mm; 附着在载体材料上的含有表面活性剂的水以完全均匀的方式储存在载体材料中，最大量为载体材料重量的80％（重量）; 并且洗涤剂由载体材料与高度浓缩的水性清洗溶液的混合物制成。该方法包括使增塑的脲醛树脂发泡体发泡的步骤; 并且通过向至少一种选自以下的溶液中加入甲醛结合物质和稳定物质来将在塑化的脲 - 甲醛树脂泡沫的发泡之前和/或期间的制造过程中存在的游离甲醛相结合：试剂溶液和树脂溶液。

35. 发明授权

US07276598B2 Phosphorylation reagents for improved processes to convert terminal hydroxyl groups of oligonucleotides into phosphate monoesters 有权
标题翻译：用于将寡核苷酸的末端羟基转化成磷酸酯单酯的改进方法的磷酸化试剂
公开(公告)号：US07276598B2
公开(公告)日：2007-10-02
申请号：US10821631
申请日：2004-04-09
申请人： Kurt Vagle , Michael Leuck , Andreas Wolter
发明人： Kurt Vagle , Michael Leuck , Andreas Wolter
IPC分类号： C07H5/06 , C07H21/04
CPC分类号： C07H5/06 , C07H21/04 , Y02P20/55
摘要： The present invention discloses novel phosphoramidite reagents for use in oligonucleotide synthesis. The present invention further discloses novel methods for the conversion of terminal hydroxyl groups of oligonucleotides into phosphate monoesters. By employing novel reagents, as also disclosed herein, the methods are fully compatible with standard procedures for solid phase oligonucleotide synthesis and do not require additional processing steps. The inventive reagents to phosphorylate terminal hydroxyl groups of oligonucleotides are superior to the prior art in that they for the first time combine the desired attributes of being a solid compound for facile handling, comprising two β-eliminating protective groups removable as fast or faster than the standard cyanoethyl group, providing a DMT-group for easy monitoring of the coupling efficiency, and enabling a fast final deprotection of the phosphorylated oligonucleotide without any extra manipulation steps.
摘要翻译：本发明公开了用于寡核苷酸合成的新型亚磷酰胺试剂。本发明还公开了将寡核苷酸的末端羟基转化成磷酸酯单酯的新方法。通过使用本文也公开的新型试剂，所述方法与固相寡核苷酸合成的标准方法完全相容，并且不需要额外的加工步骤。使寡核苷酸的末端羟基磷酸化的本发明试剂优于现有技术，因为它们首次将作为固体化合物的所需属性与易于处理组合在一起，包括可以快速或快速移除的两个β-消除保护基团标准氰乙基，提供DMT基团，以便于监测偶联效率，并且能够快速地最终去除磷酸化寡核苷酸，而无需任何额外的操作步骤。

36. 发明申请

US20190103347A1 ELECTRONIC COMPONENT ALIGNMENT DEVICE AND METHOD 审中-公开
公开(公告)号：US20190103347A1
公开(公告)日：2019-04-04
申请号：US15720606
申请日：2017-09-29
申请人： Georg Seidemann , Andreas Wolter , Stephan Stoeckl , Thomas Wagner
发明人： Georg Seidemann , Andreas Wolter , Stephan Stoeckl , Thomas Wagner
IPC分类号： H01L23/498 , H01L23/538 , H01L23/13 , H01L25/065 , H05K3/32 , H05K3/00
摘要： A system and method for aligning components is disclosed. A system arranges a plurality of components in a first component alignment. The system places two L-shaped fine placement tools in a position surrounding the plurality of components, wherein the L-shaped fine placement tools include a plurality of pins. The system applies a force to the pins included in the two L-shaped fine placement tools to shift the plurality of components from the first component alignment to a second component alignment, wherein the second component alignment has less unused space than the first component alignment. The system removes the two L-shaped fine placement tools. The system attaches the plurality of components to a carrier arranged in the second component alignment.

37. 发明申请

US20190006281A1 CROSS-CONNECTED MULTI-CHIP MODULES COUPLED BY SILICON BENT-BRIDGE INTERCONNECTS AND METHODS OF ASSEMBLING SAME 审中-公开
公开(公告)号：US20190006281A1
公开(公告)日：2019-01-03
申请号：US15637641
申请日：2017-06-29
申请人： Georg Seidemann , Bernd Waidhas , Thomas Wagner , Andreas Wolter , Laurent Millou
发明人： Georg Seidemann , Bernd Waidhas , Thomas Wagner , Andreas Wolter , Laurent Millou
IPC分类号： H01L23/538 , H01L23/498 , H01L25/065 , G11C16/18
CPC分类号： H01L23/5382 , G11C16/18 , H01L23/49861 , H01L24/16 , H01L24/32 , H01L24/75 , H01L24/81 , H01L24/83 , H01L24/92 , H01L25/0657 , H01L2224/0401 , H01L2224/16227 , H01L2224/32245 , H01L2224/4826 , H01L2224/73253 , H01L2224/81005 , H01L2224/81191 , H01L2224/83191 , H01L2224/83192 , H01L2224/92225 , H01L2924/1434 , H01L2924/15311 , H01L2924/15313
摘要： A multi-chip module includes two silicon bridge interconnects and three components that are tied together by the bridges with one of the components in the center. At least one of the silicon bridge interconnects is bent to create a non-planar chip-module form factor. Cross-connected multi-chip silicon bent-bridge interconnect modules include the two silicon bridges contacting the center component at right angles to each other, plus a fourth component and a third silicon bridge interconnect contacting the fourth component and any one of the original three components.

38. 发明申请

US20180096970A1 INTERCONNECT STRUCTURE FOR A MICROELECTRONIC DEVICE 审中-公开
公开(公告)号：US20180096970A1
公开(公告)日：2018-04-05
申请号：US15282855
申请日：2016-09-30
申请人： Klaus Reingruber , Andreas Wolter , Georg Seidemann , Thomas Wagner , Bernd Waidhas
发明人： Klaus Reingruber , Andreas Wolter , Georg Seidemann , Thomas Wagner , Bernd Waidhas
IPC分类号： H01L25/065 , H01L23/31 , H01L23/00
CPC分类号： H01L24/06 , H01L23/3128 , H01L24/13 , H01L24/14 , H01L24/17 , H01L25/117 , H01L25/50 , H01L2224/02311 , H01L2224/0237 , H01L2224/02379 , H01L2224/04105 , H01L2224/06132 , H01L2224/06134 , H01L2224/09177 , H01L2224/11334 , H01L2224/12105 , H01L2224/13024 , H01L2224/1403 , H01L2224/14051 , H01L2224/16145 , H01L2224/1703 , H01L2224/17051 , H01L2224/73204 , H01L2225/1058 , H01L2225/1064
摘要： A microelectronic package with two semiconductor die coupled on opposite sides of a redistribution layer 108, and at least partially overlapping with one another. At least a first of the semiconductor die includes two sets of contacts, the first group of contacts arranged at a lesser pitch relative to one another than are a second group of contacts. The first group of contacts at the larger pitch are placed to engage contacts in a redistribution layer 108. The second group of contacts at the lesser pitch are placed to engage respective contacts at the same pitch on the second semiconductor die.

39. 发明授权

US07427678B2 Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method 有权
标题翻译：使用环加成生物共轭法固定寡核苷酸的方法
公开(公告)号：US07427678B2
公开(公告)日：2008-09-23
申请号：US09845742
申请日：2001-05-01
申请人： Wolfgang Pieken , Andreas Wolter , David P. Sebesta , Michael Leuck , Hallie A. Latham-Timmons , John Pilon , Gregory M. Husar
发明人： Wolfgang Pieken , Andreas Wolter , David P. Sebesta , Michael Leuck , Hallie A. Latham-Timmons , John Pilon , Gregory M. Husar
IPC分类号： C07H21/00 , A61K38/00 , C07K1/00
CPC分类号： C40B50/18 , C07B2200/11 , C07C215/10 , C07C271/16 , C07C2601/16 , C07D207/46 , C07F7/1804 , C07F9/2408 , C07H21/00 , C40B40/04 , C40B40/06
摘要： This invention discloses a novel method for immobilizing molecules to a support. Specifically, this invention discloses a method of immobilizing derivatized biomolecules, such as oligonucleotides, using cycloaddition reactions, such as the Diels-Alder reaction. Included in this invention are the novel immobilized biomolecules that can be prepared according to the method of this invention.
摘要翻译：本发明公开了一种将分子固定在载体上的新方法。具体地说，本发明公开了一种使用诸如Diels-Alder反应的环加成反应固定衍生的生物分子如寡核苷酸的方法。本发明包括可根据本发明方法制备的新型固定化生物分子。

40. 发明申请

US20060247431A1 ACTIVATORS FOR OLIGONUCLEOTIDE AND PHOSPHORAMIDITE SYNTHESIS 有权
标题翻译：用于寡核苷酸和磷酰胺合成的激活剂
公开(公告)号：US20060247431A1
公开(公告)日：2006-11-02
申请号：US11380227
申请日：2006-04-26
申请人： Andreas Wolter , Michael Leuck
发明人： Andreas Wolter , Michael Leuck
IPC分类号： C07H21/02
CPC分类号： C07H21/02 , C07H21/04
摘要： The present invention discloses novel methods for the synthesis of oligonucleotides and nucleoside phosphoramidites. The methods are based on employing aryl-substituted 5-phenyl-1H-tetrazoles with perfluoroalkyl groups on the aromatic ring as activators. In one aspect the novel activators are used in the synthesis of oligonucleotides via the phosphoramidite approach. In this aspect the activators are highly efficient and can be applied with very short coupling times. In a further aspect, the activators of the invention are used in the synthesis of phosphoramidites through the reaction of nucleosides comprising a free hydroxyl group with phosphitylating agents. In this aspect the activators provide very pure phosphoramidites under mild conditions. The activators of the invention are further characterized by being highly soluble, non-hygroscopic and non-hazardous.
摘要翻译：本发明公开了合成寡核苷酸和核苷亚磷酰胺的新方法。该方法基于在芳环上使用具有全氟烷基的芳基取代的5-苯基-1H-四唑作为活化剂。在一个方面，新的活化剂用于通过亚磷酰胺方法合成寡核苷酸。在这方面，活化剂是高效的并且可以以非常短的偶联时间施用。在另一方面，本发明的活化剂用于通过包含游离羟基的核苷与磷酸化剂的反应来合成亚磷酰胺。在这方面，活化剂在温和条件下提供非常纯的亚磷酰胺。本发明的活化剂的特征还在于高溶解性，非吸湿性和非危险性。

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