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31. 发明公开

EP3433361A1 IMMOBILIZED INORGANIC PYROPHOSPHATASE (PPASE) 审中-公开
公开(公告)号：EP3433361A1
公开(公告)日：2019-01-30
申请号：EP16715810.4
申请日：2016-03-24
申请人： CureVac AG
发明人： KUNZE, Martin , HALDER, Felix Niklas , YAZDAN PANAH, Benyamin , ROOS, Tilmann
IPC分类号： C12N15/09

32. 发明公开

EP3394280A1 METHOD OF RNA IN VITRO TRANSCRIPTION USING A BUFFER CONTAINING A DICARBOXYLIC ACID OR TRICARBOXYLIC ACID OR A SALT THEREOF 审中-公开
公开(公告)号：EP3394280A1
公开(公告)日：2018-10-31
申请号：EP16831568.7
申请日：2016-12-23
申请人： CureVac AG
发明人： YAZDAN PANAH, Benyamin , WOCHNER, Aniela , ROOS, Tilmann , FUNKNER, Andreas , KUNZE, Martin
IPC分类号： C12P19/34
CPC分类号： C12Q1/6844 , C12Q2521/119 , C12Q2527/125
摘要： The present invention relates to a buffer system comprising a dicarboxylic acid or tricarboxylic acid or a salt thereof for synthesizing RNA molecules as well as a method of RNA in vitro transcription using this buffer system. The present invention also provides the use of this buffer system in RNA in vitro transcription and in the reduction or prevention of precipitates during RNA in vitro transcription.

33. 发明公开

EP3155129A1 METHODS AND MEANS FOR ENHANCING RNA PRODUCTION 有权转让
标题翻译： VERFAHREN UND MITTEL ZUR VERBESSERUNG DER RNA-HERSTELLUNG
公开(公告)号：EP3155129A1
公开(公告)日：2017-04-19
申请号：EP15738263.1
申请日：2015-06-10
申请人： CureVac AG
发明人： WOCHNER, Aniela , ROOS, Tilmann , KETTERER, Thomas
IPC分类号： C12Q1/68 , B01L3/00
CPC分类号： C12P19/34 , C12M21/18 , C12M23/44 , C12M29/04 , C12M29/14 , C12M29/18 , C12M41/26 , C12M41/32 , C12M41/48 , C12Q1/6844 , C12Q2521/119 , C12Q2525/117 , C12Q2527/113 , C12Q2527/137
摘要： The present invention relates to a method for synthesizing an RNA molecule of a given sequence, comprising the step of determining the fraction (1) for each of the four nucleotides G, A, C and U in said RNA molecule, and the step of synthesizing said RNA molecule by in vitro transcription in a sequence-optimized reaction mix, wherein said sequence-optimized reaction mix comprises the four ribonucleoside triphosphates GTP, ATP, CTP and UTP, wherein the fraction (2) of each of the four ribonucleoside triphosphates in the sequence-optimized reaction mix corresponds to the fraction (1) of the respective nucleotide in said RNA molecule, a buffer, a DNA template, and an RNA polymerase. Further, the present invention relates to a bioreactor (1) for synthesizing RNA molecules of a given sequence, the bioreactor (1) having a reaction module (2) for carrying out in vitro RNA transcription reactions in a sequence-optimized reaction mix, a capture module (3) for temporarily capturing the transcribed RNA molecules, and a control module (4) for controlling the infeed of components of the sequence-optimized reaction mix into the reaction module (2), wherein the reaction module (2) comprises a filtration membrane (21) for separating nucleotides from the reaction mix, and the control of the infeed of components of the sequence-optimized reaction mix by the control module (4) is based on a measured concentration of separated nucleotides.
摘要翻译：本发明涉及一种合成给定序列的RNA分子的方法，包括测定所述RNA分子中四个核苷酸G，A，C和U中的每一个的级分（1）的步骤，以及合成步骤所述RNA分子通过在序列优化的反应混合物中的体外转录，其中所述序列优化的反应混合物包含四种核糖核苷三磷酸GTP，ATP，CTP和UTP，其中四种核糖核苷三磷酸中的每一种的级分（2）序列优化的反应混合物对应于所述RNA分子中的相应核苷酸的级分（1），缓冲液，DNA模板和RNA聚合酶。此外，本发明涉及用于合成给定序列的RNA分子的生物反应器（1），所述生物反应器（1）具有用于在序列优化的反应混合物中进行体外RNA转录反应的反应模块（2），捕获模块（3），用于临时捕获转录的RNA分子;以及控制模块（4），用于控制将序列优化的反应混合物的组分进料到反应模块（2）中，其中反应模块（2）包含用于从反应混合物分离核苷酸的过滤膜（21），以及通过对照组（4）控制序列优化的反应混合物的组分的进料是基于分离的核苷酸的测量浓度。

34. 发明授权

EP3319622B1 METHOD FOR PRODUCING RNA MOLECULE COMPOSITIONS 有权
公开(公告)号：EP3319622B1
公开(公告)日：2020-02-12
申请号：EP16826337.4
申请日：2016-12-22
申请人： CureVac AG
发明人： ROOS, Tilmann , KUNZE, Martin , YAZDAN PANAH, Benyamin , YILMAZ, Salih , CONZELMANN, Markus
IPC分类号： A61K38/00 , A61K39/00 , A61K48/00

35. 发明公开

EP3297682A2 DRY POWDER COMPOSITION COMPRISING LONG-CHAIN RNA 有权转让
标题翻译：包含长链RNA的干粉组合物
公开(公告)号：EP3297682A2
公开(公告)日：2018-03-28
申请号：EP16725036.4
申请日：2016-05-20
申请人： CureVac AG
发明人： EBER, Fabian, Johannes , YAZDAN PANAH, Benyamin , SEWIG, Stefanie , KETTERER, Thomas , MUTZKE, Thorsten , ROOS, Tilmann , SONNTAG, Michael , WIGGENHORN, Michael , KOLLAND, Katharina
IPC分类号： A61K48/00 , C12N15/10
CPC分类号： A61K48/005 , A61K48/0091
摘要： The present invention is directed to a storage-stable formulation of long-chain RNA. In particular, the invention concerns a dry powder composition comprising a long-chain RNA molecule. The present invention is furthermore directed to methods for preparing a dry powder composition comprising a long-chain RNA molecule by spray-freeze drying. The invention further concerns the use of such a dry powder composition comprising a long- chain RNA molecule in the preparation of pharmaceutical compositions and vaccines, to a method of treating or preventing a disorder or a disease, to first and second medical uses of such a dry powder composition comprising a long-chain RNA molecule and to kits, particularly to kits of parts, comprising such a dry powder composition comprising a long-chain RNA molecule.

36. 发明公开

EP3289101A1 IMMOBILIZED POLY(N)POLYMERASE 有权转让
标题翻译：固定的聚（N）聚合酶
公开(公告)号：EP3289101A1
公开(公告)日：2018-03-07
申请号：EP16722826.1
申请日：2016-05-02
申请人： CureVac AG
发明人： ROOS, Tilmann , YAZDAN PANAH, Benyamin , CONZELMANN, Markus , WAGNER, Veronika
IPC分类号： C12Q1/68 , C12N15/10 , C12N9/12
CPC分类号： C12P19/34 , C12N9/1241 , C12N11/02 , C12N11/06 , C12N11/14 , C12N15/1006 , C12N15/113 , C12Q1/6806 , C12Y207/07019 , Y02P20/588
摘要： The present invention relates to an immobilized poly(N)polymerase (PNP), methods of producing said PNP and uses thereof. Further disclosed is an enzyme reactor and kit comprising the PNP for producing polynucleotidylated ribonucleic acid poly(N)RNA)molecules which are useful in gene therapy, immunotherapy, protein replacement therapy and/or vaccination.

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