专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

31. 发明授权

EP2683736B1 METHODS AND REAGENTS FOR CREATING MONOCLONAL ANTIBODIES 有权
标题翻译：用于创建单克隆抗体的方法和试剂
公开(公告)号：EP2683736B1
公开(公告)日：2018-01-17
申请号：EP12711501.2
申请日：2012-03-09
申请人： Cell Signaling Technology, Inc.
发明人： POLAKIEWICZ, Roberto , CHEUNG, Wan Cheung , RUSH, John Edward , BEAUSOLEIL, Sean Andre
IPC分类号： C07K16/00 , C07K16/08 , C07K16/18 , C07K16/44
CPC分类号： C07K16/00 , C07K16/082 , C07K16/18 , C07K16/44 , C07K2317/21 , C07K2317/56 , C07K2317/565
摘要： In some embodiments, the invention relates to methods for creating a monoclonal antibody that specifically binds to antigen. The method may start from a polyclonal population of antibodies such as a non-specific polyclonal population or a polyclonal population of antibodies that specifically bind to the antigen. The method includes obtaining nucleic acid molecules encoding heavy and light immunoglobulin chains (or variable regions thereof) of multiple immunoglobulins from an animal; obtaining mass spectra information of peptide fragments of a population of polyclonal immunoglobulins that specifically bind to an antigen of choice; comparing and/or correlating the mass spectra information of the peptide fragments of the polyclonal immunoglobulins with predicted mass spectra information of predicted amino acid sequences encoded by the nucleic acid molecules, and then assembling the heavy and light chains to create an antibody (or variable region thereof) that specifically binds to the antigen.

32. 发明公开

EP1904106A2 REAGENTS FOR THE DETECTION OF PHOSPHORYLATED ATR KINASE (SER 428/SER 2317) AND USES THEREOF 审中-公开
标题翻译：用于识别磷酸化ATR激酶（SER 428 / SER 2317）试剂及其用途
公开(公告)号：EP1904106A2
公开(公告)日：2008-04-02
申请号：EP06787664.9
申请日：2006-07-18
申请人： Cell Signaling Technology, Inc.
发明人： LIVINGSTONE, Mark , RUAN, Hong , POLAKIEWICZ, Roberto
IPC分类号： A61K39/395
CPC分类号： G01N33/573 , C07K16/40 , C07K16/44 , C12N9/1205 , C12Q1/485 , G01N2500/00 , Y10S435/975
摘要： The invention discloses two novel phosphorylation sites in human ATR kinase, serine 428 (Ser428) and serine 2317 (Ser2317) respectively, and provides reagents, including antibodies and AQUA peptides, that selectively bind to and/or detect ATR only when phosphorylated at one or more of these respective sites, but do not bind to ATR when not phosphorylated at these respective sites. Also provided are methods for determining the phosphorylation of ATR kinase in a biological sample, by using a detectable reagent that binds to ATR only when phosphorylated at Ser428 and/or Ser2317. Kits comprising the ATR (Ser428, Ser2317)- specific reagents of the invention are also provided.

33. 发明公开

EP1841882A1 PROTEIN PHOSPHORYLATION IN EGFR-SIGNALING PATHWAYS 审中-公开
标题翻译：蛋白激酶在EGFR信号通路中的表达
公开(公告)号：EP1841882A1
公开(公告)日：2007-10-10
申请号：EP04815061.9
申请日：2004-12-21
申请人： Cell Signaling Technology, Inc.
发明人： MORITZ, Albrecht , LI, Yu , FARNSWORTH, Charles , RIKOVA, Klarisa , LEE, Kimberly , GUO, Ailan , POLAKIEWICZ, Roberto
IPC分类号： C12Q1/48 , G01N33/68 , C07K16/00 , C07K7/00
CPC分类号： C07K16/32 , C07K16/2866 , C07K16/2869 , C07K16/40 , C07K16/44 , C07K2317/34 , G01N33/6872 , G01N33/74 , G01N2333/4706 , G01N2500/04
摘要： The invention discloses 168 novel phosphorylation sites identified in signal transduction proteins and pathways downstream of, and including, EGFR kinase, and provides phosphorylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites/proteins, as well as methods of using the reagents for such purpose. Among the phosphorylation sites identified are sites occurring in the following protein types: Actin Binding proteins, Adaptor/Scaffold proteins, Calcium-Binding Proteins, Cell Cycle Regulation proteins, Cytoskeletal proteins, DNA Binding and Replication Proteins, GTPase Activating proteins, Guanine Nucleotide Exchange Factor proteins, Lipid Kinases, Receptor Tyrosine Kinases, Receptor Tyrosine Kinase ligands, Protein Kinases, Receptor and Protein Phosphatases, Transcription Factor proteins, Tumor Suppressor proteins, and Vesicle proteins.

34. 发明公开

EP1718760A1 PROTEIN PHOSPHORYLATION IN C-SRC SIGNALING PATHWAYS 有权
标题翻译：蛋白激酶在C-SRC-SIGNALWEGEN中的应用
公开(公告)号：EP1718760A1
公开(公告)日：2006-11-08
申请号：EP04780956.1
申请日：2004-08-12
申请人： Cell Signaling Technology, Inc.
发明人： MORITZ, Albrecht , LEE, Kimberly , RUSH, John , POLAKIEWICZ, Roberto
IPC分类号： C12Q1/48 , G01N33/68 , C07K16/00 , C07K7/00
CPC分类号： G01N33/6872 , C07K14/47 , C07K16/44 , C12Q1/25 , C12Q1/485 , G01N33/6842
摘要： The invention discloses 102 novel phosphorylation sites identified in signal transduction proteins and pathways downstream of c-Src kinase, and provides phosphorylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites/proteins, as well as methods of using the reagents for such purpose. Among the phosphorylation sites identified are sites occurring in the following protein types: Adaptor/ Scaffold proteins, Actin Binding proteins, Cytoskeletal proteins, G protein/ GTPase Activating protein/Guanine Nucleotide Exchange Factor proteins, Helicases, RNA Binding proteins, Transcription/Translation Factor or Initiation Complex proteins, Cellular Metabolism Enzymes, and Vesicle proteins.

35. 发明公开

EP3345922A1 METHODS AND REAGENTS FOR CREATING MONOCLONAL ANTIBODIES 审中-公开
公开(公告)号：EP3345922A1
公开(公告)日：2018-07-11
申请号：EP17196465.3
申请日：2012-03-09
申请人： Cell Signaling Technology, Inc.
发明人： POLAKIEWICZ, Roberto , CHEUNG, Wan Cheung , RUSH, John Edward , BEAUSOLEIL, Sean Andre
IPC分类号： C07K16/00 , C07K16/08 , C07K16/18 , C07K16/44
CPC分类号： C07K16/00 , C07K16/082 , C07K16/18 , C07K16/44 , C07K2317/21 , C07K2317/56 , C07K2317/565
摘要： In some embodiments, the invention relates to methods for creating a monoclonal antibody that specifically binds to antigen. The method may start from a polyclonal population of antibodies such as a non-specific polyclonal population or a polyclonal population of antibodies that specifically bind to the antigen. The method includes obtaining nucleic acid molecules encoding heavy and light immunoglobulin chains (or variable regions thereof) of multiple immunoglobulins from an animal; obtaining mass spectra information of peptide fragments of a population of polyclonal immunoglobulins that specifically bind to an antigen of choice; comparing and/or correlating the mass spectra information of the peptide fragments of the polyclonal immunoglobulins with predicted mass spectra information of predicted amino acid sequences encoded by the nucleic acid molecules, and then assembling the heavy and light chains to create an antibody (or variable region thereof) that specifically binds to the antigen.

36. 发明授权

EP1718976B1 PROTEIN PHOSPHORYLATION IN ANAPLASTIC LARGE CELL LYMPHOMA 有权
标题翻译：蛋白质磷酸化AT大细胞间变性淋巴瘤
公开(公告)号：EP1718976B1
公开(公告)日：2008-12-10
申请号：EP04777654.7
申请日：2004-07-07
申请人： Cell Signaling Technology, Inc.
发明人： MORITZ, Albrecht , LEE, Kimberly , RUSH, John , POLAKIEWICZ, Roberto
IPC分类号： G01N33/574 , C07K16/00
CPC分类号： G01N33/6872 , C07K14/47 , C07K16/44 , C12Q1/25 , C12Q1/485 , G01N33/6842
摘要： The invention discloses 211 novel phosphorylation sites identified in signal transduction proteins and pathways underlying Anaplastic Large Cell Lymphoma (ALCL) involving the ALK-NPM translocation/fusion, and provides phosphorylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites/proteins, as well as methods of using the reagents for such purpose. Among the phosphorylation sites identified are sites occurring in the following protein types: Protein Kinases (including Receptor Tyrosine Kinases), Adaptor/Scaffold Proteins, Cellular Metabolism or Miscellaneous Enzymes, Oxidoreductases, Transcription Factors, Cytoskeletal Proteins, Translation Initiation Complexes, RNA Binding Proteins, Proteases, Acetyltransferases, G protein regulators/GTPases, Helicases, Apoptosis/Cell Cycle Regulation proteins, and Hydrolases.

37. 发明公开

EP1929296A2 REAGENTS FOR THE DETECTION OF PROTEIN PHOSPHORYLATION IN ANAPLASTIC LARGE CELL LYMPHOMA SIGNALING PATHWAYS 审中-公开
标题翻译： FOR PROTEINPHOSPHORILIERUNG IN信号的检测试剂DUE TO间变性大细胞淋巴瘤
公开(公告)号：EP1929296A2
公开(公告)日：2008-06-11
申请号：EP06803284.6
申请日：2006-09-08
申请人： Cell Signaling Technology, Inc.
发明人： POLAKIEWICZ, Roberto , LEE, Kimberly , FARNSWORTH, Charles , GUO, Ailan , MORITZ, Albrecht , SPEK, Eric , BOCCALATTE, Francesco
IPC分类号： G01N33/53
CPC分类号： G01N33/57407 , C07B59/008 , C07K16/18 , C07K16/40 , C12Q1/485
摘要： The invention discloses nearly 219 novel phosphorylation sites identified in signal transduction proteins and pathways underlying Anaplastic Large Cell Lymphoma (ALCL) involving the NPM-ALK translocation/fusion, and provides phosphorylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites/proteins, as well as methods of using the reagents for such purpose.

38. 发明公开

EP1718977A1 PROTEIN PHOSPHORYLATION IN T-CELL RECEPTOR SIGNALING PATHWAYS 有权
标题翻译：蛋白质磷酸化T细胞受体信号
公开(公告)号：EP1718977A1
公开(公告)日：2006-11-08
申请号：EP04794025.9
申请日：2004-10-04
申请人： Cell Signaling Technology, Inc.
发明人： MORITZ, Albrecht , LEE, Kimberly , RUSH, John , POLAKIEWICZ, Roberto
IPC分类号： G01N33/68 , C12Q1/48 , C07K16/00 , C07K7/00
CPC分类号： G01N33/6872 , C07K14/47 , C07K16/44 , C12Q1/25 , C12Q1/485 , G01N33/6842
摘要： The invention discloses 95 novel phosphorylation sites identified in signal transduction proteins and pathways downstream of the T-cell receptor, and provides phosphorylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites/proteins, as well as methods of using the reagents for such purpose. Among the phosphorylation sites identified are sites occurring in the following protein types: Actin Binding proteins, Adaptor/Scaffold proteins, Adhesion proteins, Calcium-binding proteins, Cell Cycle Regulation or Channel proteins, Chaperones, Cofactor proteins, Cytoskeletal proteins, DNA Binding proteins, G protein or GTPase Activating proteins, Ligases, Lipid Kinases and Binding proteins, Oxidoreductases, Protein Kinases, Protein Phosphatases, Receptor proteins, RNA Binding proteins, Transcription Factor/Initiation Complex proteins, Transcription Coactivator/Corepressor proteins, Translation Initiation Complex proteins, Ubitquitin Conjugating System proteins, and Vesicle proteins.

39. 发明公开

EP2683736A1 METHODS AND REAGENTS FOR CREATING MONOCLONAL ANTIBODIES 有权
标题翻译： VERFAHREN UND REENENZIEN ZUR ERZEUGUNG MONOKLONALERANTIKÖRPER
公开(公告)号：EP2683736A1
公开(公告)日：2014-01-15
申请号：EP12711501.2
申请日：2012-03-09
申请人： Cell Signaling Technology, Inc.
发明人： POLAKIEWICZ, Roberto , CHEUNG, Wan Cheung , RUSH, John Edward , BEAUSOLEIL, Sean Andre
IPC分类号： C07K16/00 , C07K16/08 , C07K16/18 , C07K16/44
CPC分类号： C07K16/00 , C07K16/082 , C07K16/18 , C07K16/44 , C07K2317/21 , C07K2317/56 , C07K2317/565
摘要： In some embodiments, the invention relates to methods for creating a monoclonal antibody that specifically binds to antigen. The method may start from a polyclonal population of antibodies such as a non-specific polyclonal population or a polyclonal population of antibodies that specifically bind to the antigen. The method includes obtaining nucleic acid molecules encoding heavy and light immunoglobulin chains (or variable regions thereof) of multiple immunoglobulins from an animal; obtaining mass spectra information of peptide fragments of a population of polyclonal immunoglobulins that specifically bind to an antigen of choice; comparing and/or correlating the mass spectra information of the peptide fragments of the polyclonal immunoglobulins with predicted mass spectra information of predicted amino acid sequences encoded by the nucleic acid molecules, and then assembling the heavy and light chains to create an antibody (or variable region thereof) that specifically binds to the antigen.
摘要翻译：在一些实施方案中，本发明涉及用于产生特异性结合抗原的单克隆抗体的方法。该方法可以从抗体的多克隆群体开始，例如非特异性多克隆群体或特异性结合抗原的抗体多克隆群体。该方法包括获得编码来自动物的多种免疫球蛋白的重和轻免疫球蛋白链（或其可变区）的核酸分子; 获得特异性结合所选抗原的多克隆免疫球蛋白群体的肽片段的质谱信息; 比较和/或将多克隆免疫球蛋白的肽片段的质谱信息与由核酸分子编码的预测的氨基酸序列的预测质谱信息相关联，然后组合重链和轻链以产生抗体（或可变区），其特异性结合抗原。

40. 发明公开

EP1934614A2 REAGENTS FOR THE DETECTION OF PROTEIN PHOSPHORYLATION IN CARCINOMA SIGNALING PATHWAYS 审中-公开
标题翻译：化试剂癌的蛋白质磷酸化信号检测器与
公开(公告)号：EP1934614A2
公开(公告)日：2008-06-25
申请号：EP06802734.1
申请日：2006-08-31
申请人： Cell Signaling Technology, Inc.
发明人： POLAKIEWICZ, Roberto , LEE, Kimberly , FARNSWORTH, Charles , GUO, Ailan , RIKOVA, Klarisa , LI, Yu , MORITZ, Albrecht , SPEK, Erik
IPC分类号： G01N33/574
CPC分类号： G01N33/6812 , C07K14/47 , C07K14/4748 , C07K16/44 , G01N33/57484 , G01N33/60 , G01N33/6842
摘要： The invention discloses nearly (443) novel phosphorylation sites identified in signal transduction proteins and pathways underlying human carcinoma, and provides phosphorylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites/proteins, as well as methods of using the reagents for such purpose. Among the phosphorylation sites identified are sites occurring in the following protein types: Protein kinases (including Serine/Threonine dual specificity, and Tyrosine kinases), Adaptor/Scaffold proteins, Transcription factors, Phospoatases, Tumor supressors, Ubiquitin conjugating system proteins, Translation initiation complex proteins, RNA binding proteins, Apoptosis proteins, Adhesion proteins, G protein regulators/ GTPase activating protein/ Guanine nucleotide exchange factor proteins, and DNA binding/replication/repair proteins, as well as other protein types.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式