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31. 发明申请

US20050026249A1 Enzymatically active recombinant glucocerebrosidase 审中-公开
公开(公告)号：US20050026249A1
公开(公告)日：2005-02-03
申请号：US10861041
申请日：2004-06-04
申请人： James Rasmussen , Gary Barsomian , Michel Bergh
发明人： James Rasmussen , Gary Barsomian , Michel Bergh
IPC分类号： C12N9/24 , C12N15/85 , C12N15/866 , C07H21/04 , C08B37/00 , C12N5/06 , C12N9/14 , C12P19/04
CPC分类号： C12N15/86 , A01K2217/05 , C12N9/2402 , C12N15/85 , C12N2710/14143 , C12N2830/00 , C12N2830/60 , C12N2840/20 , C12N2840/44 , C12Y302/01045
摘要： Recombinant enzymatically active glucocerebrosidase is produced by a eukaryotic cell. Also, a cell includes nucleic acid encoding enzymatically active glucocerebrosidase; also a eukaryotic organism contains such a cell. Also, a method for producing enzymatically active glucocerebrosidase includes steps of introducing glucocerebrosidase-encoding nucleic acid into a eukaryotic cell, causing the cell to express glucocerebrosidase, and purifying the glucocerebrosidase from the cell.

32. 发明授权

US06609742B1 Lifting apparatus for manipulating manhole castings and the like 失效
标题翻译：用于操纵人孔铸件等的提升装置
公开(公告)号：US06609742B1
公开(公告)日：2003-08-26
申请号：US09848488
申请日：2001-05-03
申请人： William C. Macom , Joseph H. Landry , William R. Gregor , James Rasmussen
发明人： William C. Macom , Joseph H. Landry , William R. Gregor , James Rasmussen
IPC分类号： B66C128
CPC分类号： B66C1/62 , B66C1/422 , B66C1/48
摘要： A lifting apparatus for manipulating manhole castings and the like having at least one pinch clamp with an inner leg and an outer leg opposing one another wherein the inner leg and the outer leg are engageable with the manhole casting. A foot of the pinch clamp is connected to at least one of the inner leg and the outer leg wherein the foot is wider than the width of the inner leg and the outer leg and is engageable with the manhole casting. A flexible support is pivotally connected to the pinch clamp and is connectable to a lifting device wherein the foot and the legs of the pinch clamp are engageable with the manhole casting when the flexible support is pulled taut. Preferably, the inner and outer legs are separate structures pivotally connected thereto. Alternatively, the inner and outer leg may be integrally fabricated from a one-piece structure. The flexible supports are connected to a connector which is connectable to a lifting device.
摘要翻译：一种用于操纵人孔铸件等的提升装置，其具有至少一个夹紧夹具，其具有彼此相对的内腿和外腿，其中内腿和外腿可与人孔铸件接合。夹紧夹的脚连接到内腿和外腿中的至少一个，其中脚比内腿和外腿的宽度宽，并且可与人孔铸件接合。柔性支撑件枢转地连接到夹紧夹具并且可连接到提升装置，其中当柔性支撑件被拉紧时，夹紧夹的脚部和腿部可与井眼铸造接合。优选地，内腿和外腿是与其枢转连接的分离结构。或者，内腿和外腿可以由一体式结构整体制造。柔性支撑件连接到可连接到提升装置的连接器。

33. 发明授权

US5236838A Enzymatically active recombinant glucocerebrosidase 失效
标题翻译：酶活性重组葡糖脑苷脂酶
公开(公告)号：US5236838A
公开(公告)日：1993-08-17
申请号：US748283
申请日：1991-08-21
申请人： James Rasmussen , Gary Barsomian , Michel Bergh
发明人： James Rasmussen , Gary Barsomian , Michel Bergh
IPC分类号： C12N9/24 , C12N15/56 , C12N15/85 , C12N15/866
CPC分类号： C12N15/86 , C12N15/85 , C12N9/2402 , C12Y302/01045 , C12N2710/14143 , C12N2840/20 , C12N2840/44
摘要： Recombinant enzymatically active glucocerebrosidase is produced by a eukaryotic cell. Also, a cell includes nucleic acid encoding enzymatically active glucocerebrosidase; also a eukaryotic organism contains such a cell. Also, a method for producing enzymatically active glucocerebrosidase includes steps of introducing glucocerebrosidase-encoding nucleic acid into a eukaryotic cell, causing the cell to express glucocerebrosidase, and purifying the glucocerebrosidase from the cell.
摘要翻译：重组酶活性葡糖脑苷脂酶由真核细胞产生。此外，细胞包括编码酶活性葡糖脑苷脂酶的核酸; 真核生物也含有这样的细胞。此外，生产酶活性葡糖脑苷脂酶的方法包括将葡糖脑苷脂酶编码核酸引入真核细胞，使细胞表达葡糖脑苷脂酶，并从细胞纯化葡糖脑苷脂酶的步骤。

34. 发明授权

US4844872A Fluid handling 失效
标题翻译：流体处理
公开(公告)号：US4844872A
公开(公告)日：1989-07-04
申请号：US74921
申请日：1987-07-17
申请人： Theodore S. Geiselman , James Rasmussen
发明人： Theodore S. Geiselman , James Rasmussen
IPC分类号： G01N35/10 , G01N35/08
CPC分类号： G01N35/085
摘要： A liquid handling system includes flow network module structure that defines a contained array of flow channels and a plurality of valves for controlling liquid flow through the flow channel array. The flow network module structure is adapted to be connected to an external source for applying a pressure differential to the flow network array to produce liquid flow within passages of the array. Also incorporated in the flow network module is chamber structure that is connected to the flow channel array and that has port structure in an outer surface of the module structure. Valve structure on the module structure is movable between a first position in which the port structure is closed and a second position in which the port structure is opened, the valve structure including actuator structure for moving a valve member between the first and second positions. Liquid transfer structure, including transport structure and probe structure carried on the transport structure, is adapted to cause movement of the valve structure from its first position to its second position concurrently with the movement of the probe structure into alignment with the chamber port structure for delivery of a quantity of sample material to the sample chamber and subsequent flow through the flow network array for interaction with an auxiliary fluid and transfer to an associated utilization device under the influence of an external pressure source.

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