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31. 发明授权

US07691621B2 Gene inactivated mutants with altered protein production 有权
标题翻译：具有改变的蛋白质生产的基因失活的突变体
公开(公告)号：US07691621B2
公开(公告)日：2010-04-06
申请号：US11401696
申请日：2006-04-11
申请人： Huaming Wang
发明人： Huaming Wang
IPC分类号： C12N1/00 , C12N1/15 , C12N15/63
CPC分类号： C12N1/14 , C12N9/0061 , C12N9/48 , C12N9/62 , C12N9/88 , C12N15/52 , C12P21/00 , C12P21/02 , C12Y110/03002
摘要： A recombinant filamentous fungal cell (e.g. Aspergillus) having one or more inactivated chromosomal genes is provided. The chromosomal genes in some embodiments correspond to derA, derB, htmA, mnn9, mnn10, ochA, dpp4, dpp5, pepAa, pepAb, pepAc, pepAd, pepF and combinations thereof. The recombinant fungal cells may include further inactivated chromosomal genes which correspond to pepA, pepB, pepC and pepD. The recombinant filamentous fungal cells may include a heterologous nucleic acid encoding a protein of interest. Also provided are methods of producing a protein of interest in said recombinant filamentous fungal cell.
摘要翻译：提供了具有一个或多个失活的染色体基因的重组丝状真菌细胞（例如曲霉属）。一些实施方案中的染色体基因对应于derA，derB，htmA，mnn9，mnn10，ochA，dpp4，dpp5，pepAa，pepAb，pepAc，pepAd，pepF及其组合。重组真菌细胞可以包括对应于pepA，pepB，pepC和pepD的进一步灭活的染色体基因。重组丝状真菌细胞可以包括编码目的蛋白质的异源核酸。还提供了在所述重组丝状真菌细胞中产生感兴趣的蛋白质的方法。

32. 发明授权

US07662584B2 Increased production of secreted proteins by recombinant eukaryotic cells 有权
标题翻译：通过重组真核细胞增加分泌蛋白质的产生
公开(公告)号：US07662584B2
公开(公告)日：2010-02-16
申请号：US10663450
申请日：2003-09-15
申请人： Merja E. Penttila , Michael Ward , Huaming Wang , Mari J. Valkonen , Markku L. A. Saloheimo
发明人： Merja E. Penttila , Michael Ward , Huaming Wang , Mari J. Valkonen , Markku L. A. Saloheimo
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19
CPC分类号： C12N9/16 , C07K14/37 , C12N9/12 , C12Y207/11001 , C12Y301/03016
摘要： Described herein are methods for increasing the amount of protein secreted by a cell. In one case, a cell is provided which contains a heterologous nucleic acid encoding a protein having unfolded protein response modulating activity and a heterologous nucleic acid encoding a protein of interest to be secreted. In one case, the protein having unfolded protein response modulating activity is selected from the proteins selected from the group consisting of HAC1, PTC2 and IRE1. The protein of interest can be any secreted protein such as a therapeutic or an industrial enzyme. For example the protein can be selected from the group consisting of lipase, cellulase, endo-glucosidase H, protease, carbohydrase, reductase, oxidase, isomerase, transferase, kinase, phosphatase, alpha-amylase, glucoamylase, lignocellulose hemicellulase, pectinase and ligninase.
摘要翻译：本文描述了增加细胞分泌的蛋白质的量的方法。在一种情况下，提供了包含编码具有未折叠蛋白质应答调节活性的蛋白质的异源核酸和编码目的蛋白质的异源核酸分泌的细胞。在一种情况下，具有解折叠的蛋白质反应调节活性的蛋白质选自HAC1，PTC2和IRE1中的蛋白质。感兴趣的蛋白质可以是任何分泌蛋白质，例如治疗剂或工业酶。例如，蛋白质可以选自脂肪酶，纤维素酶，内切葡糖苷酶H，蛋白酶，碳水解酶，还原酶，氧化酶，异构酶，转移酶，激酶，磷酸酶，α-淀粉酶，葡糖淀粉酶，木质纤维素半纤维素酶，果胶酶和木质素酶。

33. 发明授权

US07592157B2 Expression in filamentous fungi of protease inhibitors and variants thereof 有权
标题翻译：蛋白酶抑制剂及其变体的丝状真菌中的表达
公开(公告)号：US07592157B2
公开(公告)日：2009-09-22
申请号：US11848213
申请日：2007-08-30
申请人： Hans De Nobel , David A. Estell , Wei Liu , Scott D. Power , Brian Schmidt , Huaming Wang
发明人： Hans De Nobel , David A. Estell , Wei Liu , Scott D. Power , Brian Schmidt , Huaming Wang
IPC分类号： C07K14/42 , C07K14/81
CPC分类号： C12N15/80 , C07K14/811
摘要： Described herein are protease inhibitors, variants thereof and methods for their production.
摘要翻译：本文描述的是蛋白酶抑制剂，其变体及其制备方法。

34. 发明申请

US20080293611A1 POLYOL OXIDASES 有权
标题翻译：多环氧化物
公开(公告)号：US20080293611A1
公开(公告)日：2008-11-27
申请号：US11875788
申请日：2007-10-19
申请人： Manoj Kumar , Susan M. Madrid , Hugh C. McDonald , Ayrookaran J. Poulose , Thomas Rand , Huaming Wang
发明人： Manoj Kumar , Susan M. Madrid , Hugh C. McDonald , Ayrookaran J. Poulose , Thomas Rand , Huaming Wang
IPC分类号： C11D7/42 , C12N15/11 , C12N15/00 , C12N1/20 , C12P21/04
CPC分类号： C12N9/0006 , C07K2319/02 , C11D3/38654 , C12Y101/03
摘要： The present invention provides compositions and methods for producing a polyol oxidase in micoroorganisms, and the use of polyol oxidases in cleaning compositions. The invention includes cleaning compositions that contain combinations of two or more POx oxidases, and cleaning compositions that contain combinations of two or more POx oxidases and a perhydrolase. In particular, the invention provides methods for expressing polyol oxidases in bacterial hosts for use in detergent applications for cleaning, bleaching and disinfecting.
摘要翻译：本发明提供用于在微生物中生产多元醇氧化酶的组合物和方法，以及在清洁组合物中使用多元醇氧化酶。本发明包括含有两种或更多种POx氧化酶的组合的清洁组合物和含有两种或更多种POx氧化酶和过水解酶的组合的清洁组合物。特别地，本发明提供了用于在洗涤剂应用中用于清洁，漂白和消毒的细菌宿主中表达多元氧化酶的方法。

35. 发明申请

US20080196173A1 Novel Laccases, Compositions And Methods of Use 有权
标题翻译：新颖的漆酶，组合物和使用方法
公开(公告)号：US20080196173A1
公开(公告)日：2008-08-21
申请号：US11954804
申请日：2007-12-12
申请人： Huaming Wang , Joseph C. McAuliffe
发明人： Huaming Wang , Joseph C. McAuliffe
IPC分类号： C09B67/00 , C12N9/02 , C12N15/11 , C12S11/00 , C12N15/00 , C12N5/06
CPC分类号： D06P5/158 , C12N9/0061 , C12Y110/03002 , D06P5/13 , D06P5/132 , D06P5/137 , D06P5/15 , D06P5/153
摘要： Novel laccases, nucleic acid sequences encoding such laccases, and vectors and host cells for expressing the laccases are described. The novel laccase enzymes may be employed in conjunction with mediators to provide an improved method for bleaching denim fabrics.
摘要翻译：描述了新的漆酶，编码这种漆酶的核酸序列，以及用于表达漆酶的载体和宿主细胞。新型漆酶可以与介质结合使用，以提供改进的漂白牛仔布织物的方法。

36. 发明申请

US20080189871A1 LACCASE MEDIATORS AND METHODS OF USE 有权
标题翻译： LACCASE介质和使用方法
公开(公告)号：US20080189871A1
公开(公告)日：2008-08-14
申请号：US11954863
申请日：2007-12-12
申请人： Joseph C. McAuliffe , Huaming Wang
发明人： Joseph C. McAuliffe , Huaming Wang
IPC分类号： D06L3/11
CPC分类号： D06P5/158 , C12N9/0061 , C12Y110/03002 , D06P5/13 , D06P5/132 , D06P5/137 , D06P5/15 , D06P5/153
摘要： Novel laccase mediators, including carboxyamido and cyano derivatives of 2,6-dimethoxyphenol that exhibit improved hydrolytic stability and good bleaching performance. The novel laccase enzymes may be employed in conjunction with the 2,6-dimethoxyphenol derivatives of this invention to provide an improved method for bleaching denim fabrics.
摘要翻译：新型漆酶介质，包括显示出改进的水解稳定性和良好漂白性能的2,6-二甲氧基苯酚的羧酰氨基和氰基衍生物。新型漆酶可以与本发明的2,6-二甲氧基苯酚衍生物结合使用，以提供漂白牛仔布织物的改进方法。

37. 发明申请

US20080187964A1 ASPERGILLUS NIGER PROMOTER FOR EXPRESSING GENES IN HOST CELLS 有权
标题翻译：用于在宿主细胞中表达基因的ASPERGILLUS NIGER促进剂
公开(公告)号：US20080187964A1
公开(公告)日：2008-08-07
申请号：US12061539
申请日：2008-04-02
申请人： Steve Kim , Edwin Lee , Wei Liu , Huaming Wang
发明人： Steve Kim , Edwin Lee , Wei Liu , Huaming Wang
IPC分类号： C12P21/04 , C07H21/04 , C12N1/00 , C12N15/00
CPC分类号： C12N15/81 , C07K14/38
摘要： The present invention relates to isolated promoter sequences, particularly a promoter isolated from Aspergillus niger designated herein as A4-L or A4 and DNA constructs and vectors including the same.
摘要翻译：本发明涉及分离的启动子序列，特别是从本文称为A4-L或A4的黑曲霉分离的启动子以及DNA构建体和包含其的载体。

38. 发明申请

US20060024784A1 Phenol oxidizing enzymes 审中-公开
公开(公告)号：US20060024784A1
公开(公告)日：2006-02-02
申请号：US11130559
申请日：2005-05-17
申请人： Huaming Wang , Elizabeth Bodie
发明人： Huaming Wang , Elizabeth Bodie
IPC分类号： C12P21/06 , C07H21/04 , C12N9/02 , C12N15/74 , C12N1/16
CPC分类号： C12N9/0004 , C12N9/0071
摘要： Disclosed herein are novel phenol oxidizing enzymes encoded by nucleic acid capable of hybridizing to the nucleic acid having the sequence as shown in SEQ ID NO:1 and in particular those obtainable from fungus. The present invention provides nucleic acid sequences and amino acid sequences from Bipolaris spicifera, Curvularia pallescens and Amerosporium atrum. The present invention provides expression vectors and host cells comprising nucleic acid encoding phenol oxidizing enzymes, methods for producing the phenol oxidizing enzyme as well as methods for constructing expression hosts.

39. 发明申请

US20050153399A1 Expression in filamentous fungi of protease inhibitors and variants thereof 有权
标题翻译：蛋白酶抑制剂及其变体的丝状真菌中的表达
公开(公告)号：US20050153399A1
公开(公告)日：2005-07-14
申请号：US10971596
申请日：2004-10-22
申请人： Hans De Nobel , David Estell , Wei Liu , Scott Power , Brian Schmidt , Huaming Wang
发明人： Hans De Nobel , David Estell , Wei Liu , Scott Power , Brian Schmidt , Huaming Wang
IPC分类号： C07H21/04 , C07K14/81 , C12N1/16 , C12N9/99 , C12N15/74
CPC分类号： C12N15/80 , C07K14/811
摘要： Described herein are protease inhibitors, variants thereof and methods for their production.
摘要翻译：本文描述的是蛋白酶抑制剂，其变体及其制备方法。

40. 发明授权

US06399329B1 Phenol oxidizing enzymes 有权
标题翻译：苯酚氧化酶
公开(公告)号：US06399329B1
公开(公告)日：2002-06-04
申请号：US09468578
申请日：1999-12-21
申请人： Huaming Wang , Elizabeth A. Bodie
发明人： Huaming Wang , Elizabeth A. Bodie
IPC分类号： C12P2106
CPC分类号： C12N9/0004 , C11D3/38654 , Y10S435/911
摘要： The invention relates to phenol oxidizing enzymes encoded by nucleic acids capable of hybridizing to the nucleic acid having the sequence shown in SEQ ID NO: 1 and in particular phenol oxidizing enzymes obtainable from fungus. Particularly provided are nucleic acid sequences and amino acids from Bipolaris spicifera, Curvularia pallescens and Amerosporium atrum, and expression vectors and host cells comprising said nucleic acid sequences encoding phenol oxidizing enzymes. Additionally, methods for producing the phenol oxidizing enzymes as well as methods for constructing expression hosts are disclosed.
摘要翻译：本发明涉及能够与具有SEQ ID NO：1所示序列的核酸杂交的核酸编码的酚氧化酶，特别是可从真菌获得的酚氧化酶。特别提供的是来自Bipolaris spicifera，Curvularia pallescens和Amerosporium atrum的核酸序列和氨基酸，以及包含编码酚氧化酶的所述核酸序列的表达载体和宿主细胞。此外，公开了生产酚氧化酶的方法以及构建表达宿主的方法。

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