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    • 33. 发明公开
    • 형광염색과 이미지 분석을 통해 정량적 또는 3차원적으로표현할 수 있는 피부 각질 박리량의 평가법
    • 使用荧光染色和图像分析对人造棱镜进行定量分析或三维显示的量测量方法
    • KR1020080091901A
    • 2008-10-15
    • KR1020070034924
    • 2007-04-10
    • (주)아모레퍼시픽
    • 김은주남개원이해광김승훈안성연문성준
    • G01N33/48G01N33/533
    • A method for measuring the amount of human stratum corneum exfoliation is provided to digitize the stratum corneum exfoliation amount using an image analysis program by improving a conventional technique of evaluating the stratum corneum exfoliation amount visually through fluorescence staining, thereby verifying the stratum corneum amount objectively. A method for measuring the amount of human stratum corneum exfoliation comprises the steps of: (a) coating a fluorescent staining sample such as Dansyl chloride and dihydroxyacetone on a stratum corneum of skin surface for fluorescence-staining; (b) coating a sample for exfoliation such as glycolic acid, lactic acid, malic acid, citric acid, tartaric acid, salicylic acid and papain enzyme on the fluorescent-stained stratum corneum for 4-14 days to perform exfoliation; (c) fluorescence-photographing the exfoliated stratum corneum to obtain a fluorescent image; and (d) setting up an analysis area of the fluorescent image and digitizing fluorescence strength of the analysis by using an image analysis program. Further, the step (a) of coating a fluorescent staining sample is performed for 6 hrs.
    • 提供了用于测量人角质层脱落量的方法,通过改进通过荧光染色目视评估角质层剥离量的常规技术,通过图像分析程序对角质层去角质量进行数字化,从而客观地验证角质层数量。 用于测量人角质层剥离量的方法包括以下步骤:(a)将荧光染色样品如丹酰氯和二羟基丙酮涂覆在皮肤表面的角质层上用于荧光染色; (b)在荧光染色的角质层上涂覆乙醇酸,乳酸,苹果酸,柠檬酸,酒石酸,水杨酸和木瓜蛋白酶等剥离性样品4-14天,进行剥离; (c)荧光拍摄剥离的角质层以获得荧光图像; 和(d)通过使用图像分析程序设置荧光图像的分析区域和数字化分析的荧光强度。 此外,涂布荧光染色样品的步骤(a)进行6小时。
    • 34. 发明公开
    • 자외선 차단제의 in vitro 워터 레지스턴스 SPF측정 시스템 및 이를 이용한 SPF 측정 방법
    • 用于测量SUNSCREEN药剂的耐水性SPF的体系及其使用方法测定SUNSCREEN AGENT的体外耐水性SPF
    • KR1020070106080A
    • 2007-11-01
    • KR1020060038518
    • 2006-04-28
    • (주)아모레퍼시픽
    • 안성연양혜진박재희이해광문성준
    • G01M99/00
    • A system for measuring the in-vitro water resistance SPF of a sunscreen agent and a method for measuring the SPF by using the same are provided to predict the water resistance degree of a product without performing an actual clinic test. A system for measuring the in-vitro water resistance SPF of a sunscreen agent includes a drive section(10), and a rotary blade(20). The drive section includes a rotational shaft(11). The rotational shaft is submerged in the water in a washing tub. The rotary blade is installed at one end of the rotational shaft. An ultraviolet sunscreen agent is coated on a surface of the rotary blade. The ultraviolet sunscreen agent is washed out in the water by rotating the rotary blade driven by the drive section. The rotary blade has a plurality of blade ribs(22) extended vertically at one end of the rotational shaft, and substrates(24) coated with the ultraviolet sunscreen agent.
    • 提供了一种用于测量防晒剂的体外抗水性SPF的系统和使用该防晒剂的SPF测量方法,以预测产品的耐水性,而不进行实际临床试验。 用于测量防晒剂的体外防水性SPF的系统包括驱动部分(10)和旋转叶片(20)。 驱动部分包括旋转轴(11)。 旋转轴浸入洗涤桶中的水中。 旋转叶片安装在旋转轴的一端。 紫外线防晒剂涂覆在旋转刀片的表面上。 通过旋转由驱动部驱动的旋转刀片,紫外线防晒剂在水中洗出。 旋转刀片具有在旋转轴的一端处垂直延伸的多个叶片肋(22)和涂覆有紫外线防晒剂的基底(24)。
    • 35. 发明公开
    • 항노화 효과를 나타내는 히드록삼산 유도체 및 이의제조방법
    • 羟脯氨酸衍生物显示抗体效应,其制备方法和用于外用的皮肤制剂和包含衍生物的胶原蛋白和激酶表达抑制剂
    • KR1020050022251A
    • 2005-03-07
    • KR1020040020401
    • 2004-03-25
    • (주)아모레퍼시픽
    • 노호식백흥수김수정채병근이병석김배환최규호손의동이해광김덕희장이섭이옥섭
    • C07C239/14
    • PURPOSE: Provided are a hydroxamic acid derivative which shows an antiaging effect and is improved in safety and stability to the skin stimulus and discoloration, its preparation method, and an antiaging skin preparation for external use and a collagenase and elastase expression inhibitor containing the derivative. CONSTITUTION: The hydroxamic acid derivative is represented by the formula 1, wherein R1 and R2 are H, a C1-C10 alkyl group or a C3-C6 cycloalkyl group; R3 is CONH, NHCO, CONR5 or NR5CO (wherein R5 is H or a C1-C10 alkyl group); and R4 is -(CH)n- (wherein n is 0 or 1). The method comprises the steps of reacting benzoic acid and methyl 4-aminobenzoate or 4-aminophenylacetic acid methyl ester or reacting aniline and monomethyl terephthalate, to prepare a benzamide compound; substituting the amino bond of the benzamide compound into an alkyl bond; hydrolyzing the methyl ester group to prepare an acid; and reacting the obtained acid with hydroxyl amine·HCl salt to prepare the hydroxamic acid derivative of the formula 1.
    • 目的:提供一种异羟肟酸衍生物,其表现出抗衰老作用,并且改善了皮肤刺激和变色的安全性和稳定性,其制备方法和外用抗衰老皮肤制剂以及含有该衍生物的胶原酶和弹性蛋白酶表达抑制剂。 构成:异羟肟酸衍生物由式1表示,其中R 1和R 2是H,C 1 -C 10烷基或C 3 -C 6环烷基; R3是CONH,NHCO,CONR5或NR5CO(其中R5是H或C1-C10烷基); 且R 4为 - (CH)n - (其中n为0或1)。 该方法包括使苯甲酸和4-氨基苯甲酸甲酯或4-氨基苯乙酸甲酯或苯胺与对苯二甲酸单甲酯反应制备苯甲酰胺化合物的步骤; 将苯甲酰胺化合物的氨基取代成烷基键; 水解甲基酯以制备酸; 并使所得酸与羟胺·HCl盐反应制备式1的异羟肟酸衍生物。