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31. 发明申请

US20060068394A1 Method of producing a DNA library using positional amplification 审中-公开
标题翻译：使用位置扩增产生DNA文库的方法
公开(公告)号：US20060068394A1
公开(公告)日：2006-03-30
申请号：US10798025
申请日：2004-03-11
申请人： John Langmore , Vladimir Makarov
发明人： John Langmore , Vladimir Makarov
IPC分类号： C40B40/08 , C12Q1/68 , C12P19/34
CPC分类号： C12N15/1093 , C12N15/10 , C12N15/1096 , C12P19/34 , C12Q1/6844 , C12Q1/6855 , C40B40/00 , C12Q2525/155 , C12Q2521/319 , C12Q2521/101 , C12Q2533/101 , C12Q2531/113 , C12Q2525/191
摘要： The disclosed invention relates to general and specific methods to use the Primer Extension/Nick Translation (PENT) reaction to create an amplifiable DNA strand, called a PENTAmer. A PENTAmers can be made for the purpose of amplifying a controlled length of DNA located at a controlled position within a DNA molecule, a process referred to as Positional Amplification by Nick Translation (PANT). In contrast to PCR, which amplifies DNA between two specific sequences, PANT can amplify DNA between two specific positions. PENTAmers can be created to amplify-very large regions of DNA (up to 500,000 bp) as random mixtures (unordered positional libraries), or as molecules sorted according to position (ordered positional libraries). PANT is fast and economical, because PENTAmer preparation can be multiplexed. A single PENTAmer preparation can include very complex mixtures of DNA such as hundreds of large-insert clones, complete genomes, or cDNA libraries. Subsequent PCR amplification of the preparation using a single specific primer can positionally amplify contiguous regions along a specific clone, along a specific genomic region, or along a specific expressed sequence.
摘要翻译：所公开的发明涉及使用引物延伸/翻译（PENT）反应产生称为PENTAmer的可扩增DNA链的一般和具体方法。可以制备PENTAmers用于扩增位于DNA分子内的受控位置的受控长度的DNA，通过Nick Translation（PANT）称为位置扩增的过程。与PCR扩增两个特定序列之间的DNA相比，PANT可以在两个特定位置之间扩增DNA。可以创建PENTAmers来扩增非常大的DNA区域（高达500,000bp）作为随机混合物（无序位置文库），或作为根据位置（有序位置文库）分类的分子。 PANT是快速和经济的，因为PENTAmer准备可以复用。单一的PENTAmer制剂可以包括非常复杂的DNA混合物，例如数百个大插入克隆，完整的基因组或cDNA文库。随后使用单一特异性引物进行PCR制备的PCR扩增可以沿着特定克隆，沿着特定基因组区域或沿特定表达序列位置扩增连续区域。

32. 发明申请

US20050128434A1 Computerized virtual reflex perimetry 审中-公开
标题翻译：电脑虚拟反射视野
公开(公告)号：US20050128434A1
公开(公告)日：2005-06-16
申请号：US10946949
申请日：2004-09-22
申请人： Tsontcho Ianchulev , Peter Pham , Vladimir Makarov
发明人： Tsontcho Ianchulev , Peter Pham , Vladimir Makarov
IPC分类号： A61B3/024 , A61B3/02 , A61B3/00 , A61B13/00
CPC分类号： A61B3/024
摘要： An automated unaided virtual perimetry system and underlying method for relative-threshold contrast sensitivity interrogation of the visual field via algorithmic presentation of visual stimuli on a screen and subsequent analysis of test response data for the detection of pathophysiologic scotomas as well response time registration and analysis for visual motor reflex testing. Launch of system via access from a remote server or direct installation of systems technology allows seamless capability at the local computer system to instruct, administer, and analyze output of stimuli and input of responses of test subjects or subjects independent of external input or control. Specific algorithmic test environment is designed to achieve comparable clinical efficacy and diagnostic utility to office-based perimetry system.
摘要翻译：一种自动化的无辅助虚拟视野系统和通过屏幕上的视觉刺激的算法演示的视野的相对阈值对比度灵敏度询问的基础方法，随后分析用于检测病理生理学阴影的测试响应数据以及响应时间注册和分析视觉运动反射测试。通过远程服务器的访问启动系统或直接安装系统技术，可以使本地计算机系统的无缝功能指导，管理和分析刺激的输出以及与外部输入或控制无关的测试对象或主体的响应输入。具体的算法测试环境旨在实现与基于办公室的视野测量系统相当的临床效果和诊断效用。

33. 发明申请

US20050053986A1 Compositions and methods for analysis of nucleic acids 有权
标题翻译：用于分析核酸的组合物和方法
公开(公告)号：US20050053986A1
公开(公告)日：2005-03-10
申请号：US10890483
申请日：2004-07-13
申请人： Vladimir Makarov , John Langmore
发明人： Vladimir Makarov , John Langmore
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6855 , C12Q1/6869 , C12Q2521/301 , C12Q2521/319 , C12Q2531/113
摘要： Disclosed are a number of methods that can be used in a variety of embodiments, including, creation of a nucleic acid terminated at one or more selected bases, sequence analysis of nucleic acids, mapping of sequence motifs within a nucleic acid, positional mapping of nucleic acid clones, and analysis of telomeric regions. The methods utilize double-stranded templates, and in most aspects involve a strand replacement reaction initiated at one or more random or specific locations created in a nucleic acid molecule, and in certain aspects utilizing an oligonucleotide primer.
摘要翻译：公开了可以用于各种实施方案中的多种方法，包括在一个或多个选择的碱基上终止的核酸的产生，核酸的序列分析，核酸内的序列基序的映射，核酸的位置定位酸克隆和端粒区域分析。该方法利用双链模板，并且在大多数方面涉及在核酸分子中产生的一个或多个随机或特定位置处引发的链置换反应，并且在某些方面使用寡核苷酸引物。

34. 发明申请

US20050032104A1 Genome walking by selective amplification of nick-translate DNA library and amplification from complex mixtures of templates 审中-公开
标题翻译：通过选择性扩增切口翻译DNA文库并从模板的复杂混合物中扩增的基因组
公开(公告)号：US20050032104A1
公开(公告)日：2005-02-10
申请号：US10909516
申请日：2004-08-02
申请人： Vladimir Makarov , Emmanuel Kamberov , Irina Sleptsova
发明人： Vladimir Makarov , Emmanuel Kamberov , Irina Sleptsova
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6869 , C12Q1/6876
摘要： Improved methods and reagents for chromosome walking of nucleic acid are discussed herein. A library of amplifiable nick translation molecules is generated, and a chromosome walk is initiated from a known sequence in the nucleic acid by producing at least one nick translate molecule, sequencing part of the nick translate molecule, and producing a second nick translate molecule by initiating the primer extension from the region of the obtained sequence of the prior nick translate molecule.
摘要翻译：本文讨论了用于核酸染色体行走的改进方法和试剂。产生可扩增切口平移分子的文库，通过产生至少一个切口平移分子，切割平移分子的测序部分，并通过引发产生第二切口平移分子，从核酸中的已知序列引发染色体步态来自所获得的先前切口平移分子序列的区域的引物延伸。

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