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    • 36. 发明专利
    • Device for distributing illumination light and detection light in microscope and its method
    • 用于分布照明灯的设备和微光检测灯及其方法
    • JP2013057931A
    • 2013-03-28
    • JP2012178023
    • 2012-08-10
    • Leica Microsystems Cms Gmbhライカ マイクロシステムス ツェーエムエス ゲーエムベーハー
    • SCHREIBER FRANK
    • G02B21/00G01N21/64
    • G02B21/0032G02B21/0068G02B21/0076G02B21/16G02B27/283
    • PROBLEM TO BE SOLVED: To provide a device for distributing illumination light and detection light in a microscope, and its method.SOLUTION: The device has a light distribution optical system 12 that guides illumination light 14 to a sample 18 and guides detection light 20 from the sample 18 to at least one of detectors. The light distribution optical system 12 comprises: polarization units 28, 30, and 32 arranged in a first optical path and configured to convert the illumination light 14 directed to the sample 18 into a first polarization state; a beam splitter 34 having such polarization dependability that while the illumination light 14 converted into the first polarization state is guided to the sample 18, a first portion 20a of detection light from the sample 18, which exhibits a first polarization state, is guided so as to return to the first optical path, a second portion 20b of the detection light, which has a second polarization state different from the first polarization state, is guided to a second optical path; and a beam combiner 38 configured to combine the first portion 20a and the second portion 20b of the detection light together and to guide the combined portions to the detector.
    • 要解决的问题:提供一种用于在显微镜中分配照明光和检测光的装置及其方法。 解决方案:该装置具有将照明光14引导到样品18并将检测光20从样品18引导到至少一个检测器的配光系统12。 光分配光学系统12包括:布置在第一光路中并被配置为将指向样本18的照明光14转换成第一偏振状态的偏振单元28,30和32。 具有这种极化依赖性的分束器34,当将转换成第一偏振态的照明光14引导到样品18时,来自样品18的具有第一偏振态的检测光的第一部分20a被引导为 为了返回到第一光路,具有与第一偏振态不同的第二偏振状态的检测光的第二部分20b被引导到第二光路; 以及组合器38,被配置为将检测光的第一部分20a和第二部分20b组合在一起,并将组合部分引导到检测器。 版权所有(C)2013,JPO&INPIT
    • 37. 发明专利
    • Scanning microscope and method for imaging object with optical microscope
    • 扫描显微镜和用光学显微镜成像对象的方法
    • JP2013003585A
    • 2013-01-07
    • JP2012133714
    • 2012-06-13
    • Leica Microsystems Cms Gmbhライカ マイクロシステムス ツェーエムエス ゲーエムベーハー
    • KNEBEL WERNERULRICH HEINRICH
    • G02B21/06
    • G02B21/367G02B21/0032G02B21/0076G02B21/10G02B21/16
    • PROBLEM TO BE SOLVED: To improve a scanning microscope so that an optical microscope is able to perform imaging free from collision even in a difficult geometric condition.SOLUTION: In order to incline each illuminating focus (16, 84) with respect to the optical axis (O) of an illuminating optical system (10), a scanner (33) directs an illuminating light beam (12) to a partial area away from the pupil center of the incident pupil (14) of the illuminating optical system (10). Also, in order to move the illuminating focus (16, 84) over an illuminating target area, the scanner changes the direction of incidence of the illuminating light beam (12) within the partial area. An observation objective lens (38) spatially separated from the illuminating optical system (10) is provided. The observation objective lens (38) is arranged so that its optical axis (O) is located substantially perpendicular to the illuminating target area and so that this axis forms an acute angle (α) with respect to the optical axis (O) of the illuminating optical system (10).
    • 要解决的问题:为了改善扫描显微镜,使得光学显微镜即使在困难的几何条件下也能够进行没有碰撞的成像。 解决方案:为了相对于照明光学系统(10)的光轴(O 1 )倾斜每个照明聚焦(16,84),扫描仪 (33)将照明光束(12)引导到远离照明光学系统(10)的入射光瞳(14)的光瞳中心的部分区域。 此外,为了将照明聚焦(16,84)移动到照明目标区域上,扫描器改变部分区域内的照明光束(12)的入射方向。 提供了与照明光学系统(10)在空间上分离的观察物镜(38)。 观察物镜38设置为使其光轴(O 3 )基本上垂直于照明目标区域,并且使得该轴形成锐角(α )相对于照明光学系统(10)的光轴(O 1 )。 版权所有(C)2013,JPO&INPIT
    • 38. 发明专利
    • Method and apparatus for imaging sample with scanning microscopy
    • 用扫描显微镜成像样品的方法和装置
    • JP2012145939A
    • 2012-08-02
    • JP2012001076
    • 2012-01-06
    • Leica Microsystems Cms Gmbhライカ マイクロシステムス ツェーエムエス ゲーエムベーハー
    • BIRK HOLGERWIDZGOWSKI BERNDHOLGER NISSLE
    • G02B21/00G01N21/64G01N21/88
    • G02B21/008G01N21/6458G02B21/0076
    • PROBLEM TO BE SOLVED: To provide a method and an apparatus which image a sample with a scanning microscopy.SOLUTION: Disclosed are a method and an apparatus for imaging a sample (28) with a scanning microscopy. Multiple sample points are scanned with a scanning beam (14) in consecutive scanning time sections. An intensity of light emitted from each of the scanned sample points is sensed repeatedly within each of the related scanning time sections. Based on intensities sensed at each of the scanned sample points, an intensity average value is found as an average value image point signal. Then, by synthesizing such average value image point signals, an average value raster image signal is generated. Further, in addition, based on intensities sensed at each of the scanned sample points, an intensity variance value is found as a variance image point signal. Then, by synthesizing such variance image point signals, a variance raster image signal is generated.
    • 要解决的问题:提供一种用扫描显微镜对样品进行成像的方法和装置。 解决方案:公开了一种用扫描显微镜对样品(28)进行成像的方法和装置。 在连续扫描时间段内用扫描光束(14)扫描多个采样点。 在每个相关的扫描时间段内重复地感测从每个扫描的采样点发射的光的强度。 基于在每个扫描采样点处感测到的强度,发现强度平均值作为平均值图像点信号。 然后,通过合成这样的平均值图像点信号,生成平均值光栅图像信号。 此外,另外,基于在每个扫描采样点处感测到的强度,发现强度方差值作为方差图像点信号。 然后,通过合成这样的方差图像点信号,生成方差光栅图像信号。 版权所有(C)2012,JPO&INPIT